Preparation method of composition containing probiotics and unsaturated fatty acids and application of composition
A technology of unsaturated fatty acids and probiotics, applied in the field of probiotics product preparation, can solve the problems of reducing the viable rate of probiotics, increasing the oxidation of unsaturated fatty acids, denaturation degree, low embedding rate, etc., to maintain low water activity , the nutrition remains intact, the method is simple and effective
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Embodiment 1
[0026] Soybean protein solution with a concentration of 15 w% was prepared as the wall material solution, and the freeze-dried powder of Lactobacillus delbrueckii subsp. 13 CFU / mL of core liquid. The wall material solution and the core material liquid were simultaneously extruded from the coaxial nozzle through a syringe pump, electrostatically sprayed in a high-voltage electric field with a voltage of 60 KV, the receiving distance was 15 cm, and the relative humidity was 45%. The microcapsule is microcapsule with lactobacillus delbrueckii subsp. bulgaricus and arachidonic acid composition as the core and soybean protein as the shell, and the particle diameter of the microcapsule is 2-10 microns. The composition has no bad taste of unsaturated fatty acids, 90% of the Lactobacillus delbrueckii remains active after being stored at normal temperature for 12 months, and the arachidonic acid is basically not oxidized.
Embodiment 2
[0028]Prepare the chitosan / inulin solution with a concentration of 2 w% as the wall material solution, add the freeze-dried powder of Bifidobacterium infantis into DHA, and use the homogeneous dispersion method to prepare a probiotic concentration of 10 10 CFU / mL of core liquid. The wall material solution and the core material liquid were extruded from the coaxial nozzle through the syringe pump at the same time, and sprayed electrostatically in a high-voltage electric field with a voltage of 25 KV, the receiving distance was 25 cm, and the relative humidity was 40%, and a complete core / shell structure was prepared. The microcapsule takes the composition of bifidobacterium infantis and DHA as the core and the chitosan / inulin as the shell, and the particle size of the microcapsule is 1-5 micrometers. The composition has no bad taste of unsaturated fatty acids, and 88% of bifidobacteria retain activity after being stored at normal temperature for 12 months, and the DHA is basic...
Embodiment 3
[0030] Prepare the collagen / alginic acid solution with a concentration of 3 w% as the wall material solution, add the freeze-dried powder of Lactobacillus plantarum, Bifidobacterium bifidum and Streptococcus thermophilus into linolenic acid, and use the homogeneous dispersion method to prepare probiotics Bacterial concentration is 10 11 CFU / mL core material liquid, the ratio of the three probiotics is 1:1:1. The wall material solution and the core material liquid were extruded from the coaxial nozzle through the syringe pump at the same time, and sprayed electrostatically in a high-voltage electric field with a voltage of 25 KV, the receiving distance was 25 cm, and the relative humidity was 40%, and a complete core / shell structure was prepared. The microcapsule is composed of Lactobacillus plantarum, Bifidobacterium bifidum, Streptococcus thermophilus and linolenic acid as the core, and collagen / alginic acid as the shell, and the particle size of the microcapsule is 2-15 mic...
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