Preparation method of genipin

A kind of genipin and nanotechnology, applied in the field of bioengineering, can solve the problems of poor dispersion of immobilized enzymes and low enzyme loading, and achieve the effects of reducing production costs, large enzyme loading, and improving use efficiency

Active Publication Date: 2017-05-24
NANJING UNIV OF SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Common immobilization carriers include chitosan, calcium alginate, etc. Compared with nanomaterials, there are problems such as poor dispersion of immobilized enzymes and low enzyme loading (Yang YS, et al. Transformation of geniposide into genipin by immobilized β- glucosidase in a two-phase aqueous-organic system[J].Molecules,2011,16(5):4295-4304.)

Method used

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  • Preparation method of genipin
  • Preparation method of genipin

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Experimental program
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Embodiment 1

[0016] Nano SiO 2 The amination modification of particles can refer to the literature (Huang, J, et al. A new immobilized glucose oxidase using SiO 2 nanoparticles as carrier[J]. Materials Science and Engineering C,2011,31(7):1374-1378.).

[0017] 40mg nano SiO 2 Disperse the particles in 4mL of absolute ethanol by ultrasonic wave, add 80μL of 3-aminopropyltriethoxysilane (APTES), mix thoroughly, and react for 24h in a shaker at 30℃ and 120r / min to complete nano-SiO 2 The amination process of the particles to obtain Nano-SiO 2 @NH 2 . After the reaction, the suspension was centrifuged at 8000×g for 3 min, the supernatant was discarded to collect the precipitate, and the precipitate was washed 5 times with distilled water. After the precipitate was fully resuspended in 4mL phosphate buffer (10mM, pH 7.3), 1mL of 25% glutaraldehyde solution was added and reacted for 2h in a shaker at 30℃ and 120r / min to complete the aminated carrier and glutaraldehyde. Cross-linking reaction to get...

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Abstract

The invention discloses a preparation method of genipin. The preparation method comprises the following steps: carrying out glutaraldehyde crosslinking on amination-modified nano SiO2 particles and beta-glucosaccharase to obtain a nano immobilized enzyme, and carrying out high-efficiency hydrolysis on the substrate jasminoidin by using the nano immobilized enzyme to obtain the genipin. The nano immobilized enzyme prepared in the method has favorable dispersity and is recyclable in the reaction system; and the method has the advantages of high genipin yield and purity, and thus, can effectively prepare genipin.

Description

Technical field [0001] The invention belongs to the technical field of biological engineering, and specifically relates to a preparation method of genipin. Background technique [0002] The free enzyme is catalyzed under the most suitable conditions for the enzyme reaction, and it has defects such as easy inactivation and failure to recover after the reaction, and the free enzyme is not easily separated from the product, which affects the purification and quality of the product. The immobilization of enzymes can effectively solve the above problems. The immobilized enzyme not only has the catalytic properties of the enzyme, but also can be separated from the reaction liquid by filtration or centrifugation after the reaction, and can be used repeatedly. Enzymes can be immobilized by different carriers and methods. After the enzyme is immobilized, the enzymatic properties including temperature, pH, and stability are quite different from the free enzyme. Nano SiO 2 The particle si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C12N11/14
CPCC12N9/2445C12N11/14C12P17/06C12Y302/01021
Inventor 李大力张璇杨成丽陈杏鲍炳鑫
Owner NANJING UNIV OF SCI & TECH
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