Preparation method of genipin
A kind of genipin and nanotechnology, applied in the field of bioengineering, can solve the problems of poor dispersion of immobilized enzymes and low enzyme loading, and achieve the effects of reducing production costs, large enzyme loading, and improving use efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0016] Nano SiO 2 The amination modification of particles can refer to the literature (Huang, J, et al. A new immobilized glucose oxidase using SiO 2 nanoparticles as carrier[J]. Materials Science and Engineering C,2011,31(7):1374-1378.).
[0017] 40mg nano SiO 2 Disperse the particles in 4mL of absolute ethanol by ultrasonic wave, add 80μL of 3-aminopropyltriethoxysilane (APTES), mix thoroughly, and react for 24h in a shaker at 30℃ and 120r / min to complete nano-SiO 2 The amination process of the particles to obtain Nano-SiO 2 @NH 2 . After the reaction, the suspension was centrifuged at 8000×g for 3 min, the supernatant was discarded to collect the precipitate, and the precipitate was washed 5 times with distilled water. After the precipitate was fully resuspended in 4mL phosphate buffer (10mM, pH 7.3), 1mL of 25% glutaraldehyde solution was added and reacted for 2h in a shaker at 30℃ and 120r / min to complete the aminated carrier and glutaraldehyde. Cross-linking reaction to get...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com