Efficient immobilized enzyme column and preparation method and application thereof

An immobilized enzyme, high-efficiency technology, applied in the direction of immobilization on or in the inorganic carrier, immobilized on/in the organic carrier, etc., can solve the incompatibility of rapid material transport and high surface activity, single pore size distribution, and porous Inconvenient use of materials, etc., to achieve the effect of reducing the reaction time of enzymolysis, low gelling temperature, and easy absorption and infiltration

Inactive Publication Date: 2019-09-20
南京萌萌菌业有限公司
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention: Aiming at various defects existing in the current immobilized enzyme and the preparation process, the present invention provides a high-efficiency immobilized enzyme column, which has excellent pore characteristics, large specific surface area, and cross-linked immobilized enzyme on the surface of the pore. Molecules, increase the amount of enzyme loaded, improve the rate of enzymatic hydrolysis, and solve the problem of continuous reaction of immobilized enzymes
Overcome many problems such as single pore size distribution of particle and film-like immobilized enzymes, incompatibility between rapid material transport and high surface activity, inconvenient use of porous materials, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Efficient immobilized enzyme column and preparation method and application thereof
  • Efficient immobilized enzyme column and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: Prepare the immobilized enzyme column according to the following method:

[0041] 1. Raw material preparation and prefabrication

[0042] The raw material of the liquid substrate is fresh milk, the viscosity at 37°C is 2.5 centipoise, and the active biological enzyme adopts lactase to hydrolyze the lactose in the fresh milk.

[0043] Weigh the agarose dry powder, the balance is pure water, add the agarose dry powder into pure water and mix well, heat to 90°C and mix well, keep warm at 60°C for later use, and prepare agarose solution with a final concentration of 0.05wt% according to the above method And final concentration 1.0wt% agarose solution;

[0044] 2. Preparation of high-efficiency immobilized enzyme column:

[0045] (1) Take 3 to 5-year-old moso bamboo trunks as raw materials, raise the temperature at 6°C / min to a carbonization temperature of 700°C under a nitrogen atmosphere, and heat-preserve and carbonize for 2 hours to obtain a bamboo charco...

Embodiment 2

[0055] Embodiment 2: Prepare the immobilized enzyme column according to the following method:

[0056] 1. Raw material preparation and prefabrication

[0057] The raw material of the liquid substrate is chemically pure olive oil with a viscosity of 80 centipoise at 30°C. The active biological enzyme adopts fat triglyceride lipase to hydrolyze the ester bonds in the olive oil.

[0058] Weigh agarose dry powder, 1wt% PEG6000, and the balance is pure water. Add the agarose dry powder into pure water and mix well, heat to 90°C and mix well, keep warm at 70°C for later use, and prepare the final concentration of 0.3 wt% agarose solution and final concentration 1.5wt% agarose solution;

[0059] When preparing the agarose solution, add PEG4000 or PEG6000 as a porogen;

[0060] 2. Preparation of high-efficiency immobilized enzyme column:

[0061] (1) Take aged grass stalks of Imperata cottage as raw material, raise the temperature to carbonization temperature of 900°C at 9°C / min un...

Embodiment 3

[0072] Embodiment 3: Prepare the immobilized enzyme column according to the following method:

[0073] 1. Raw material preparation and prefabrication

[0074] The raw material of the liquid substrate is a soluble starch solution, and the viscosity is adjusted to 60 centipoise with pure water at 30°C. The active biological enzyme uses α-amylase to hydrolyze the starch to generate glucose and maltose.

[0075] Weigh agarose dry powder, 2wt% PEG4000, and the balance is pure water, add the agarose dry powder into pure water and mix well, heat to 90°C and mix well, keep warm at 65°C for later use, and prepare 0.17wt% by the above method Agarose solution and 1.25wt% agarose solution;

[0076] 2. Preparation of high-efficiency immobilized enzyme column:

[0077] (1) Take Chinese fir peeled wood as a raw material, heat up to a carbonization temperature of 800° C. at 7.5° C. / min under a nitrogen atmosphere, and heat-preserve and carbonize for 2 hours to obtain a charcoal porous carbo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses an efficient immobilized enzyme column and a preparation method and application thereof. The efficient immobilized enzyme column comprises a porous base material and active biological enzymes, a porous structure of the porous base material allows simultaneous distribution of large holes, medium holes and small holes, the large holes, the medium holes and the small holes are communicated to form three-dimensional through structures, and the active biological enzymes are fixed to surfaces of the three-dimensional through structures according to a glutaraldehyde crosslinking method. The porous base material is prepared by loading of agarose gel on porous carbon material powder and is filled in situ and condensed in a columnar mould and capable of immobilizing and loading various active biological enzymes such as protease, lactase, lipase and amylase, and the enzyme loading capacity of the porous base material is 133-216IU / g. The porous carbon material provides a large specific area, the high enzyme loading capacity can be achieved after crosslinking with the biological enzymes, high bioactivity of the biological enzymes after long-time enzymolysis treatment can be guaranteed, requirements on stability and continuity of enzymolysis treatment can be met, and production efficiency in biological enzyme treatment is improved substantially.

Description

technical field [0001] The invention relates to the field of immobilized enzymes, in particular to a high-efficiency immobilized enzyme column and its preparation method and application. Background technique [0002] Enzyme immobilization refers to the use of some carrier materials to bind or limit the enzyme to a certain area, and the enzyme can still perform its unique catalytic reaction, and can be recycled and reused. The immobilized enzyme refers to an enzyme that is locked in a certain space and still retains its catalytic activity, and can be used continuously or repeatedly. [0003] In enzyme immobilization reactions, the most commonly used cross-linking agent is glutaraldehyde because it has two functionally active aldehyde groups. During the reaction, an aldehyde group of glutaraldehyde will interact with -NH of chitosan on the surface of the carrier 2 Cross-linking with -OH, and its other functionally active aldehyde group will interact with -NH on the surface o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10
CPCC12N11/10C12N11/14
Inventor 孙有俊孙冬梅范彩云鲍芳丽
Owner 南京萌萌菌业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap