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60results about How to "No effect on activity" patented technology

Flue gas denitration catalyst and preparation method and application thereof

The invention discloses a flue gas denitration catalyst. The catalyst comprises a carrier and a catalytic activity component, wherein the catalytic activity component is manganese, cobalt and cerium composite oxide, wherein the molar ratio of the manganese element to the cobalt element to the cerium element is 1:0.1-1:0.1-1. A preparation method of the flue gas denitration catalyst comprises the following steps of: (1) taking a soluble manganese salt, a soluble cobalt salt and a soluble cerium salt, wherein the molar ratio of the manganese element to the cobalt element to the cerium element is 1:0.1-1:0.1-1, dissolving the salts in deionized water, and adding ammonia water to adjust the pH value to between 5 and 7; (2) soaking a carrier in aqueous solution of citric acid, diluted hydrochloric acid or diluted nitric acid for 24 to 48 hours, drying the carrier, soaking the carrier in the solution of the step (1), standing the carrier for 24 hours and drying the carrier; and (3) roastingthe prepared sample in step (2) in air, and cooling the sample. The flue gas denitration catalyst has the advantages of simple preparation method, high poisoning resistance, no secondary pollution toenvironment, wide activity temperature window, low activity starting temperature and the like.
Owner:SHANDONG GEMSKY ENVIRONMENTAL PROTECTION TECH CO LTD

Method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot

The invention relates to a method for extracting procyanidine from fruit or pomace of aronia melanocarpa elliot. The method comprises the steps of dipping the fruit or pomace of the aronia melanocarpa elliot into an organic solvent, conducting Flash extraction for 0.5-30 minutes by using a Flash extractor, carrying out suction filtration and centrifugation to obtain a procyanidine extract solution of the aronia melanocarpa elliot; concentrating the procyanidine extract solution of the aronia melanocarpa elliot by using a negative pressure rotary evaporation device to obtain procyanidine extract concrete of the aronia melanocarpa elliot; and after pre-freezing the procyanidine extract concrete of the aronia melanocarpa elliot by using an ultra-low temperature refrigerator, carrying out freeze drying on the procyanidine extract concrete by using a freeze drying device to obtain the freeze-dried powder of a procyanidine extract of the aronia melanocarpa elliot. According to the method disclosed by the invention, low temperature in the whole course can be realized, procyanidine disintegration caused by high temperature can be avoided, the loss of anthocyanin in the extracting process effectively can be effectively avoided, the operation is simple and the cost is low, and promotion in enterprises can be achieved by forming an automatic production line, so that the method has a relatively high commercial value.
Owner:LIAONING UNIVERSITY

Homogeneous immunodetection kit for detecting anti-cyclic citrullinated peptide antibody and application thereof

The invention provides a homogeneous immunodetection kit for detecting an anti-cyclic citrullinated peptide antibody. The kit is based on a double antigen sandwich reaction mode and prepared by adopting high sensitivity and specificity of cyclic citrullinated peptide-coupled carrier protein as an antigen raw material; and the kit can detect all types of anti-citrulline antibodies and has the advantages of being strong in specificity, good in signal amplification effect, high in sensitivity, wide in linearity range, simple in operation, good in stability and high in precision.
Owner:CHEMCLIN DIAGNOSTICS CO LTD

Lead ion visual detection method

The invention discloses a lead ion fast visual detection method. The method comprises: performing hybrid hybridization of fundamental chains and enzyme chains to form a GR-5 DNA enzyme; adding oxidized graphene for treatment to remove unreacted free single-stranded nucleic acid to eliminate the false positive effect; spraying two kinds of gold label probes on a gold label mat; and adding two chains which are obtained from the GR-5 DNA enzyme which is a pretreated to-be-detected sample through cutting at the rA position, wherein the two chains together with the gold label probes and nucleotide sequences located on a detection line form a sandwich structure through chromatography, so that the chains are fixed at the detection line for color development, and excessive free nucleic acid is fixed at a quality control line for color development. According to the intensity of color development on the detection line, the lead ion concentration is obtained. Lead ions can be detected in 15 min at the room temperature, and the minimum limit of detection of the lead ions can reach 0.05 nM. The method is high in sensitivity and good in specificity, is not affected by other divalent metal ions, is simple and easy to operate, and can be directly applied to detection of lead ions in the environment.
Owner:HUAZHONG UNIV OF SCI & TECH

Attenuated salmonella typhimurium mediated eukaryocyte plasmid transfection method

The invention provides an attenuated salmonella typhimurium mediated eukaryocyte plasmid transfection method. According to the transfection method, based on an intracellular invasion characteristic of the attenuated salmonella typhimurium, recombinant plasmids are carried to enter eukaryocytes to realize specific protein expression. Specifically, recombinant eukaryotic expression plasmids are introduced into an attenuated salmonella typhimurium VNP20009 strain in an electric shock transformation way; then recombinant bacteria and the eukaryocytes are cultured in a co-incubation manner; and finally, the attenuated salmonella typhimurium inside and outside cells is killed by a serum culture medium containing penicillin and streptomycin, and the recombinant plasmids are released to the eukaryocytes to realize the specific protein expression. Compared with conventional calcium sulfate transfection, liposome transfection and electro-transformation, the transfection method disclosed by the invention takes viable bacteria as a bearing host of specific plasmids, so that a larger number of target vectors can be obtained by only conventional culture; and in addition, the plasmid transfection operating steps are simplified, the cost is lowered, and the transfection efficiency and the cytotoxicity are comprehensively better than those in the conventional transfection method.
Owner:NANCHANG UNIV

Separation and purification method of umbilical cord mesenchymal stem cells

The invention relates to a separation and purification method of umbilical cord mesenchymal stem cells and belongs to the technical field of stem cells and regenerative medicine. The method comprisesthe steps as follows: separation of umbilical cord tissue: taking the umbilical cord tissue, cleaning the umbilical cord tissue and removing blood, and shearing the tissue into tissue fragments for later use; tissue digestion: taking the tissue fragments, and adding tissue digestive juice for oscillation digestion, wherein the tissue digestive juice contains neutral protease, hyaluronidase, collagenase II and DNA enzyme; after digestion, adding a stop buffer for stop, performing filtration to obtain undigested tissue blocks and filtrate, and centrifuging the filtrate to remove supernatant to obtain cells; and cell culture: inoculating a culture flask with the tissue blocks and cells, using serum-free culture medium for culture to obtain the umbilical cord mesenchymal stem cells. The separation and purification method of umbilical cord mesenchymal stem cells overcomes the defects of an enzyme digestion method and a tissue block culture method in the prior art and can obtain enough primary cells within 3-5 days.
Owner:GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD

Immunity enhancing health product and preparation method thereof

The present invention relates to an immunity enhancing health product and a preparation method thereof, wherein the raw materials comprise, by weight, 230-250 parts of cervus elaphus linnaeus, 6-18 parts of a ginseng ethanol extract, 5-15 parts of a ginseng residue water extract, and 28-48 parts of an ophiopogon japonicus water extract. The preparation method comprises: (1) crushing ginseng into coarse grains, carrying out reflux extraction with ethanol, filtering, concentrating the filtrate, carrying out vacuum drying, and crushing so as to be spare; (2) adding water to the residue obtained in the step (1), cooking, filtering, concentrating the filtrate, carrying out vacuum drying, and crushing so as to be spare; (3) crushing ophiopogon japonicus into coarse grains, adding water, cooking, filtering, concentrating the filtrate, carrying out vacuum drying, and crushing so as to be spare; (4) removing villus from cervus elaphus linnaeus in a burning manner, completely scraping, splitting into broken blocks, drying, placing to achieve a cold state, crushing into fine powder, and sterilizing so as to be spare; and (5) weighing the raw materials according to a certain weight part ratio, uniformly mixing, and tableting or loading into capsules. The immunity enhancing health product of the present invention has advantages of simple formula and human body immunity improving.
Owner:吉林鹿司令健康产业有限公司

Application of liriope spicata saponin B in preparation of medicine for treating skin inflammation

The invention relates to the technical field of biological medicines, in particular to application of liriope spicata saponin B in preparation of a medicine for treating skin inflammation. The research finds that various inflammatory cytokines can induce skin cell inflammatory reaction, so that the levels of CCL5, CCL17 and CCL22 are significantly increased. HaCaT cells are treated by using the liriope spicata saponin B, so that the levels of CCL5, CCL17 and CCL22 can be reduced, the inflammatory reaction induced by combination of the inflammatory cytokines can be inhibited, and a new way is provided for research and development of a new medicine for resisting the skin inflammation induced by various inflammatory cytokines.
Owner:WUYI UNIV +1

Self-stabilizing cervical interbody fusion cage and manufacturing die and method thereof

The invention relates to a self-stabilizing cervical interbody fusion cage and a manufacturing die and method thereof. The self-stabilizing cervical interbody fusion cage comprises a fusion cage body made of mineralized glue and an anterior cervical approach fixing plate made of medical degradable polymer materials, the fusion cage body is in a wedge shape, the front portion of the fusion cage body is high, the rear portion of the fusion cage body is low, the anterior cervical approach fixing plate is located at the front end of the fusion cage body and is integrally formed with the fusion cage body, and the upper end and the lower end of the anterior cervical approach fixing plate are respectively provided with a fixing hole used for fixation of the cervical vertebra. The anterior cervical approach fixing plate and the fusion cage body are integrally formed so that the whole self-stabilizing cervical interbody fusion cage can be fixed to the upper cervical vertebra body and the lower cervical vertebra body and can be prevented from slipping off forwards or sliding backwards, and accordingly the fusion cage can be prevented from pressing nervous centralis; the fusion cage body made of the mineralized glue can be degraded and absorbed and can guide generation of new bones, the anterior cervical approach fixing plate made of the medical degradable polymer materials can be automatically degraded after an operation, and the trouble that another operation needs to be carry out to take out the fusion cage body is avoided.
Owner:杨述华 +1

Application of complanatoside in preparation of medicine for treating skin inflammation

The invention relates to the technical field of biological medicines, in particular to application of complanatoside in preparation of a medicine for treating skin inflammation. The research finds that various inflammatory cytokines can induce skin cell inflammatory response, so that the levels of IL-1beta, ROS and CCL22 are obviously increased; and the levels of IL-1beta, ROS and CCL22 can be reduced by treating HaCaT cells with the complanatoside, the inflammatory reaction induced by the combination of inflammatory cytokines can be inhibited, and a new way is provided for research and development of new drugs for resisting skin inflammation induced by various inflammatory cytokines.
Owner:WUYI UNIV
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