The invention relates to a production and freeze-drying method and application for supernate of a culture solution of a mesenchymal stem cell. a culture solution supernate freeze drying agent can be used with skin care products simultaneously after being dissolved again, the function of improving a skin is achieved, the production method adopts umbilical cord tissues of a healthy person as raw materials, the umbilical cord tissues are digested through collagenase and cultured outside the body, amplification and trypsin digestive passage are conducted, an NuncTM cell factory system with ten layers or four layers is utilized to replace a traditional cell culture bottle finally, the cost is greatly reduced, needed space is reduced, a large number of mesenchymal stem cells are obtained in shorter culture time, low concentration fetal bovine serum (3%FBS) is reserved in cell culture media to make the condition of the cells healthier compared with that of the cells without serum, the quality of culture supernatant fluid is guaranteed, the culture supernatant fluid can be stored under room temperature after vacuum freeze drying is conducted on the culture supernatant fluid directly, and activity of various growth factors of the culture supernatant fluid is maintained.