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Umbilical cord tissue cryopreservation and resuscitation method

A kind of umbilical cord tissue and tissue technology, which is applied in the direction of bone/connective tissue cells, human or animal body preservation, embryonic cells, etc. It can solve the problems of long operation time and high necrosis rate of umbilical cord tissue, so as to improve production rate and cryopreservation and recovery effect, the effect of shortening the cooling time

Active Publication Date: 2018-06-08
SHANGHAI CRYOWISE MEDICAL TECH CO LTD +1
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Problems solved by technology

[0006] The present invention provides a simple and effective cryopreservation and resuscitation method for umbilical cord tissue to obtain mesenchymal stem cells, avoiding the problems of long operation time and high necrosis rate of umbilical cord tissue existing in the prior art

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  • Umbilical cord tissue cryopreservation and resuscitation method
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  • Umbilical cord tissue cryopreservation and resuscitation method

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Embodiment Construction

[0038] Unless otherwise defined, the technical terms or scientific terms used in the claims and description of the present invention shall have the ordinary meanings understood by those skilled in the technical field to which the present invention belongs.

[0039] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the present invention will be further described below in conjunction with specific embodiments. It should be noted that the following embodiments are intended to facilitate understanding of the present invention, and the present invention is claimed The scope is not limited in any way. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0040] In the following examples, unless otherwise specified, the methods used are conventional methods, and the...

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Abstract

The invention provides an umbilical cord tissue cryopreservation and resuscitation method. The method comprises the following steps: S1, cutting umbilical cord tissues to obtain first tissue strips; S2, immersing the first tissue strips in a vitrification solution, and then directly cryopreserving the first tissue strips to obtain second tissue strips; and S3, resuscitating the second tissue strips to obtain third tissue strips, wherein the vitrification solution includes a first vitrification solution, a second vitrification solution and a third vitrification solution, and the step S2 includes the following processes: sequentially immersing the first tissue strips in the first vitrification solution, the second vitrification solution and the third vitrification solution, transferring thefirst tissue strips into liquid nitrogen, and cryopreserving the first tissue strips to obtain the second tissue strips. The umbilical cord strips are cryopreserved and resuscitated through a vitrification technology, so the operation time is shortened, the cryopreservation efficiency is improved, and the necrosis rate of the umbilical cord tissues in the cryopreservation and resuscitation processis reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cryopreservation and resuscitation method for umbilical cord tissue and a method for separating mesenchymal stem cells. Background technique [0002] The umbilical cord is an important substance connecting the mother and the fetus during the fetal period. It is a cord-like structure, surrounded by a layer of amniotic membrane, containing two umbilical arteries and one umbilical vein. There is a special embryonic mucus-like umbilical cord tissue between the blood vessels and the amniotic membrane. Mesenchymal stem cells isolated from umbilical cord tissue can be used to treat various diseases. [0003] The commonly used method to obtain mesenchymal stem cells is to separate the mesenchymal stem cells from the umbilical cord tissue for cryopreservation, and then thaw them before use. Resuscitation and separation of umbilical cord tissue can save time and reduce the diffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073C12N5/0775A01N1/02
CPCA01N1/0221A01N1/0226C12N5/0605C12N5/0668
Inventor 杨秋蕊朱雪晶翟志敏冯炜炜许啸声吴红平
Owner SHANGHAI CRYOWISE MEDICAL TECH CO LTD
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