Novel immobilized enzyme preparation, and preparation method and application thereof
An immobilized enzyme, a new type of technology, applied in the direction of being immobilized on/in an organic carrier, can solve the problems of complex enzyme immobilization method, difficult to recycle, small load capacity, etc., and achieve high immobilization efficiency and high enzyme performance. Good stability and mild reaction effect
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Embodiment 1
[0043] (1) Chitosan is dissolved in acetic acid solution to obtain chitosan acetic acid solution, wherein the massfraction of chitosan is 3%, and the massfraction of acetic acid is 5%; then add tetraoxide in chitosan acetic acid solution Three ferromagnetic fluid, 45 ℃ of ultrasonic reactions 50min, obtain product 1, wherein, the mass ratio of ferroferrofluid and chitosan acetic acid solution is 1:3; Product 1 is added liquid paraffin / Span-80 mixed liquor ( The volume ratio of liquid paraffin and Span-80 is 25:1), and the volume ratio of product 1 and liquid paraffin / Span-80 mixture is 1:4; Add the crosslinking agent glutaraldehyde under the condition (the consumption of glutaraldehyde is 8% of total reaction system volume), and adjust the pH of the reaction system to be 10, continue the reaction in a constant temperature water bath for 1.5h; then filter under reduced pressure, and use petroleum ether, Extracted with absolute ethanol 4 times, dried in vacuum at 50°C to obtain ...
Embodiment 2
[0049] (1) chitosan is dissolved in acetic acid solution to obtain chitosan acetic acid solution, wherein the massfraction of chitosan is 1%, and the massfraction of acetic acid is 1%; then add tetraoxide in chitosan acetic acid solution Three ferromagnetic fluid, 40 ℃ of ultrasonic reactions 60min, obtain product 1, wherein, the mass ratio of ferroferrofluid and chitosan acetic acid solution is 1:2; Product 1 is added liquid paraffin / Span-80 mixed liquor ( The volume ratio of liquid paraffin and Span-80 is 30:1), and the volume ratio of product 1 and liquid paraffin / Span-80 mixture is 1:5; Add cross-linking agent glutaraldehyde under the condition (the consumption of glutaraldehyde is 5% of total reaction system volume), and adjust reaction system pH to be 11, constant temperature water bath continues reaction 2h; Extracted with water and ethanol for 3 times, dried in vacuum at 30°C to obtain nano-magnetic chitosan;
[0050] (2) Dissolve γ-cyclodextrin and potassium benzoate...
Embodiment 3
[0055] (1) chitosan is dissolved in acetic acid solution to obtain chitosan acetic acid solution, wherein the massfraction of chitosan is 5%, and the massfraction of acetic acid is 10%; then add tetraoxide in chitosan acetic acid solution Three ferrofluid, 50 ℃ of ultrasonic reactions 40min, obtain product 1, wherein, the mass ratio of ferroferrofluid and chitosan acetic acid solution is 1:5; Product 1 is added liquid paraffin / Span-80 mixed liquor ( The volume ratio of liquid paraffin and Span-80 is 20:1), and the volume ratio of product 1 and liquid paraffin / Span-80 mixture is 1:3; Add cross-linking agent glutaraldehyde (the consumption of glutaraldehyde is 10% of total reaction system volume) under condition, and adjust reaction system pH to be 9, constant temperature water bath continues reaction 1h; Extracted with water and ethanol for 5 times, dried in vacuum at 60°C to obtain nano-magnetic chitosan;
[0056] (2) Dissolve γ-cyclodextrin and potassium benzoate in water at...
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