Poly(methacrylic acid-itaconic acid) modified membrane, as well as preparation method and application thereof

A technology of methacrylic acid and itaconic acid, which is applied in the direction of immobilization on/in organic carriers, fermentation, etc., can solve the problem of low efficiency of immobilized enzymes, achieve improved utilization of enzymes, simple process, and high selectivity sexual effect

Active Publication Date: 2019-06-28
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is less efficie

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of poly(methacrylic acid-itaconic acid) film

[0026] Poly(methacrylic acid-itaconic acid) membrane was prepared by phase inversion method: 15 g poly(methacrylic acid-itaconic acid) powder [n(IA):n(MAA)=1:10] was mixed with 5 mL porogen poly Add vinylpyrrolidone (PVP-K30) to 60mL N,N-dimethylformamide, mix well, seal and react at 60°C for 48h, form a white viscous liquid, take it out, cool at room temperature for 12h to remove air bubbles; Spread it in a glass plate tank with a depth of 1 mm, evaporate at room temperature for 2 minutes, and then transfer to a coagulation bath (DMF / water solution = 1:10, 25°C) to cast a film for 5 minutes. Cut the film to 15cm 2 stand-by. Samples were dried and weighed. The porosity of the poly(methacrylic acid-itaconic acid) film prepared according to the method is 0.80, and the maximum mechanical tensile force it can bear is 6.13N.

[0027] (2) Immerse the poly(methacrylic acid-itaconic acid) film obtained in step (...

Embodiment 2

[0031] (1) Preparation of poly(methacrylic acid-itaconic acid) film

[0032] Poly(methacrylic acid-itaconic acid) membrane was prepared by phase inversion method: 15 g poly(methacrylic acid-itaconic acid) powder [n(IA):n(MAA)=1:7] was mixed with 10 mL porogen poly Add vinylpyrrolidone (PVP-K25) to 65mL N,N-dimethylformamide, mix well, seal and react at 65°C for 45h, take it out after forming a white viscous liquid, cool at room temperature for 12h to remove bubbles; Spread in a glass plate tank with a depth of 1 mm, evaporate at room temperature for 3 minutes, and then transfer to a coagulation bath (DMF / water solution = 1:8, 25°C) for 5 minutes to cast a film. Cut the film to 15cm 2 stand-by. Samples were dried and weighed. The porosity of the poly(methacrylic acid-itaconic acid) film prepared according to the method is 0.88, and the maximum mechanical tensile force it can withstand is 5.83N.

[0033] (2) Immerse the poly(methacrylic acid-itaconic acid) film obtained in s...

Embodiment 3

[0037] (1) Preparation of poly(methacrylic acid-itaconic acid) film

[0038] Poly(methacrylic acid-itaconic acid) membrane was prepared by phase inversion method: 15 g poly(methacrylic acid-itaconic acid) powder [n(IA):n(MAA)=1:5] was mixed with 7 mL porogen poly Add vinylpyrrolidone (PVP-K30) to 70mL N,N-dimethylformamide, mix well, seal the reaction at 60°C for 50h, take it out after forming a white viscous liquid, and cool it at room temperature for 12h to remove air bubbles; Spread it in a glass plate tank with a depth of 0.8 mm, evaporate at room temperature for 3 minutes, and then transfer to a coagulation bath (DMF / water solution = 1:7, 25°C) to cast a film for 5 minutes. Cut the film to 15cm 2 stand-by. Samples were dried and weighed. The porosity of the poly(methacrylic acid-itaconic acid) film prepared according to the method is 0.93, and the maximum mechanical tensile force it can bear is 5.54N.

[0039] (2) Immerse the poly(methacrylic acid-itaconic acid) film ...

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Abstract

The invention provides a poly(methacrylic acid-itaconic acid) modified membrane, as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing poly(methacrylic acid-itaconic acid)(IA-MAA), polyvinylpyrrolidone (PVP) and N,N-dimethyl formamide to obtain a casting solution; reacting the casting solution in a sealed condition at 55-65 DEG C for40-50 hours, defoaming, and preparing a flat membrane by adopting a tape casting method; soaking the flat membrane with the casting solution in a gel bath, and curing to form a membrane. The poly(methacrylic acid-itaconic acid) modified membrane prepared by a conversion method by using immobilized carrier as a phase, lipase is efficiently immobilized by modifying chitosan and activating glutaraldehyde. The method is simple in operation, the material has stable performance, the selectivity of transesterification can be greatly improved. The poly(methacrylic acid-itaconic acid) modified membranehas excellent industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, and in particular relates to a poly(methacrylic acid-itaconic acid) modified membrane, a preparation method thereof and an application in transesterification. Background technique [0002] Lipase is a general term for acyl hydrolases that can catalyze the hydrolysis of fat. It can catalyze organic synthesis reactions such as ester hydrolysis, alcoholysis, ester synthesis, transesterification and stereoisomer resolution on the water interface. It is used in the food industry, It has important applications in many fields such as detergent, oil industry, pharmaceutical industry, agriculture and environmental governance. As a green biocatalyst, lipase has a series of advantages such as high catalytic activity, less side reactions, low pollution, and high operational stability. However, in practical industrial applications, lipase also has some limitations, such as: in high temperature, ...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08J5/18C08J9/42C08J9/40C12N11/08C12P7/62C08L33/02
Inventor 孙伯旺陈方慧王明亮周钰明李瑶嘉朱岭军
Owner SOUTHEAST UNIV
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