The invention discloses a method for preparing an AIDS immunoadsorption therapeutic cell line in the biomedical field. The method is characterized in that CD4+T cells and macrophages are sorted by animmunomagnetic bead method and are placed in a pre-fusion agent containing PHA, concanavalin A, IFN-gamma, SP2 / 0-IgG and CD4-IgG with myeloma cells (SP2 / 0), under combined action of PHA-activated T cells, concanavalin A for change of cell membrane solubility and an IFN-gamma activated macrophage IgGFc receptor, the IgGFab segment of CD4-IgG is bonded to CD4+T cells having CD4 epitope, the IgGFc segment is bonded to macrophages with IgGFc receptors, the IgGFab segment of SP2 / 0-IgG is bonded to the SP2 / 0 and the IgGFc segment is bonded to macrophages with IgGFc receptors so that a CD4+T cell-CD4-IgG-macrophage-IgG-SP2 / 0 conjugate is formed, three types of cells are fused under the action of a fusion agent, and the fused cells are cultured by a cell fusion selection medium (HAT) so that the AIDS immunoadsorption therapeutic cell line with effects of long-term passage, production of cytokines and HIV endocytosis is acquired.