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Method for preparing glucose-sensitive insulin controlled-release biological material

A glucose-sensitive, biological material technology is applied in the field of preparation of dextran-concanavalin A-based insulin self-controlled biological carrier material, can solve problems such as difficulty in realizing large-scale production, and achieves easy and The effect of mild conditions and excellent self-controlled release performance

Active Publication Date: 2010-06-23
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this polymerization method is not yet easy to achieve large-scale production, and the sensitivity and self-controlled release performance of the obtained carrier system still need to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dissolve 5.4g dextran (molecular weight 10kDa) and 0.8g methacrylic anhydride in 80mL dimethyl sulfoxide, add 0.08g 4-dimethylaminopyridine, react at 48°C for 36h, use deionized water and The dialysis membrane was dialyzed for 7 days and freeze-dried to obtain a methallyl dextran monomer with a substitution degree of 16%.

[0021] Dissolve 1 g of concanavalin A and 0.1 g of methacrylic anhydride in 24 mL of phosphate buffer (20 mM, pH 7.4) and react at 51 °C for 2 h, then dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 8 kDa for 7 days, freeze-dried to obtain methallyl concanavalin A monomer.

[0022] Take 50 mg of the above-mentioned methallyl dextran monomer, 25 mg of the above-mentioned methallyl concanavalin A monomer, dissolve in 1.5 mL of phosphate-citric acid buffer (50 mM, pH 5), and obtain a single body solution; add 7.5 mg of ammonium persulfate and 9.4 mg of tetramethylethylenediamine to the monomer solution, react at ...

Embodiment 2

[0025] Dissolve 5.5g dextran (molecular weight 100kDa) and 1.3g methacrylic anhydride in 100mL dimethyl sulfoxide, add 0.26g 4-dimethylaminopyridine, react at 55°C for 12h, use deionized water and The dialysis membrane was dialyzed for 7 days and freeze-dried to obtain a methallyl dextran monomer with a substitution degree of 12%; the preparation of the methallyl concanavalin A monomer was the same as in Example 1.

[0026] Take 50 mg of the above-mentioned allyl dextran monomer and 50 mg of the above-mentioned methallyl concanavalin A monomer, and dissolve them in 1 mL of Tris-hydrochloric acid buffer (10 mM, pH 9) to obtain a monomer solution; Add 2mg of ammonium persulfate and 10mg of tetramethylethylenediamine to the monomer solution, react at 60°C for 5min, dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 10kDa for 3 days, remove unreacted substances, and obtain sensitive self-controlled release of glucose Biomaterials for insulin. ...

Embodiment 3

[0029] Dissolve 6 g of dextran (molecular weight 70 kDa) and 1.2 g of acrylic anhydride in 100 mL of dimethyl sulfoxide, add 0.18 g of 4-dimethylaminopyridine, react at 50 °C for 24 h, and dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 10 kDa After 7 days, it was freeze-dried to obtain an allyl dextran monomer with a substitution degree of 18%.

[0030] Dissolve 1 g of concanavalin A and 0.1 g of acrylic anhydride in 24 mL of phosphate buffer (20 mM, pH 7.4) and react at 51 °C for 2 h, then dialyze with deionized water and a dialysis membrane with a molecular weight cut-off of 8 kDa for 7 days. Freeze-dry to obtain allyl concanavalin A monomer.

[0031]Take 75mg of the above-mentioned allyl dextran monomer and 75mg of the above-mentioned allyl concanavalin A monomer, dissolve in 1mL Tris-hydrochloric acid buffer (10mM, pH 8) to obtain a monomer solution; Add 7.5 mg of ammonium persulfate and 3 mg of tetramethylethylenediamine to the so...

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Abstract

The invention discloses a new method for preparing an intelligent glucose-sensitive insulin controlled-release biological material. The method comprises the following steps of: firstly, dissolving glucose derivative monomers with C=C derivatives and concanavalin A derivative monomers with C=C double bonds in buffer solution to produce monomer solution; secondly, adding a catalyst in the monomer solution to perform free radical polymerization at a certain temperature; and finally, removing unreacted reactant through dialysis to obtain the glucose-sensitive insulin controlled-release biologicalmaterial. The preparation method has the advantages of convenient implementation, fast reaction process, mild reaction condition and easy production preparation on a large scale. The obtained glucan-concanavalin A insulin controlled-release biological carrier material has the advantages of good glucose-sensitive property and excellent insulin controlled-release performance.

Description

(1) Technical field [0001] The invention relates to a method for preparing a glucose-sensitive biomaterial capable of self-controlling release of insulin, in particular to a new method for preparing a glucose-sensitive dextran-concanavalin A-based insulin self-controllable release biocarrier material, The invention belongs to the technical field of drug controlled release and biomaterials. (2) Background technology [0002] Insulin-dependent diabetes mellitus (type I) is a systemic chronic metabolic system disease, which is very harmful to human health. Controlled release of insulin is an effective method for the treatment of type 1 diabetes. In recent years, the self-regulating plant lectin-saccharide derivative intelligent insulin delivery carrier system developed for the research and development of type I diabetes provides a new way for the treatment of diabetes. The carrier system mainly uses the specific binding properties of plant lectins (such as concanavalin A) and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/36A61K38/28A61P5/48C08B37/02
Inventor 张玮沈绍传
Owner ZHEJIANG UNIV OF TECH
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