209results about How to "Increase proliferative activity" patented technology

The invention relates to a dietary fiber food with various functional actions. The compound dietary fiber food is formed by preparing and processing the adopted main materials comprising 2% to 10% of oligopolymerization xylose, 15% to 50% of poly-glucose, 3.3% to 10% of rivier giantarum refined powder, 10% to 50% of soybean dietary fiber and 10% to 50% of microcrystalline cellulose, and auxiliary materials comprising 0.5% to 2% of citric acid, 0.2% to 0.6% of aspartame and 0.5% to 1.8% of powder essence. Various types of plant fiber give full play to synergistic actions and advantage complementation; by using the compound cellulose, the system improves the function and the metabolism of large intestines, promotes the creepage and the water absorption expansion of intestinal tracts, quickens defecation, maintains the probiotic environment of the intestinal tracts, comprehensively regulates the cholesterol level, prohibits the absorption of a human body to cholesterol, bile acid, heavy metal and edible pigment and reinforces the immunity of the human body.

The present disclosure provides methods for genetically modifying lymphocytes and methods for performing adoptive cellular therapy that include transducing T cells and/or NK cells without prior ex vivo stimulation. The methods typically include engineered signaling polypeptides that can include a lymphoproliferative element, and/or a chimeric antigen receptor (CAR), for example a microenvironment restricted CAR. Additional elements of such engineered signaling polypeptides are provided herein, as well as vectors, such as retroviral vectors, packaging cell lines and methods of making the same. Furthermore, recombinant retroviruses and methods of making the same are provided. Numerous controls are provided, including riboswitches that are controlled, for example in vivo, by nucleoside analogues.

The invention provides a preparation method of universal heterologous CAR-T cells and an application. Constructed CD28-CD137-CD19-CD3 full-length gene is introduced into allogeneic T cells of healthy persons with CRISPR/Cas9 technology, in order to produce CAR-T cells with targeting cytotoxicity; after amplification in vitro, the cells are fed back to patients for carrying out antineoplastic treatment, and in the treatment process, a PNAs method is used for removing lymphocyte in order to avoid antigraft reaction of hosts. The chimeric antigen receptors can be prepared in large scale with T lymphocyte of healthy human, and antineoplastic treatment of heterologous cancer patients.

A tissue-engineered artificial skin able to regulate the pigment secretion is configured through externally culturing the ceratin cells, fibroblasts and melanocyte of skin, proportionally mixing by tissue engineering method, and forming artificial skin.

The invention discloses a human umbilical cord mesenchymal stem cell serum-free culture medium and a preparation method thereof, and an acquisition method of a human umbilical cord mesenchymal stem cell serum-free culture solution. The culture medium comprises a basic culture medium and a serum-free culture supernatant concentrated solution, and also comprises the following components and concentrations: 5-100 ng/ml of a vitamin composition, 50-200 [mu]g/ml of grape polyphenol, 1-50 ng/ml of a recombinant human epidermal growth factor, 1-50 ng/ml of a recombinant human basic fibroblast growthfactor, 1-10 ng/ml of a recombinant platelet-derived growth factor-C, 1-10 ng/ml of a recombinant human insulin-like growth factor-1, 50-200 [mu]M of beta-mercaptoethanol, 1-100 [mu]g/ml of human serum albumin, 1-100 [mu]g/ml of glutathione, and 1.2-3.7 g/L of sodium bicarbonate. The human umbilical cord mesenchymal stem cell serum-free culture medium is used for replacing a traditional culture medium containing animal serum to culture human umbilical cord mesenchymal stem cells, and unsafe factors such as human immune response caused by stem cell pollution by pathogens or animal-derived components and heterologous protein are avoided.

The invention discloses a total nutrient meal suitable for being eaten by patients suffering from tumor, which comprises the following components by weight percent: 10-20% of puffing powders, 10-20% of bean flour, 15-25% of maltodextrin, 3-6% of lactalbumin, 3-6% of hydrolyzed lactalbumin, 8-15% of whole milk powders, 3-5% of a medium-chain fatty acid, 3-6% of black sesame oil, 0.8-1.3% of deep sea fish oil, 0.5-1% of reishi shell-broken spore powder, 0.2-0.8% of longan polysaccharides, 0.05-0.1% of arginine, 5% of inulin and 3-6% of powdered sugar. The nutrient meal disclosed by the invention contains the longan polysaccharides, the ganoderma lucidum spores powder, the arginine and the like, so that the nutrient meal has a certain effect of inhibiting the proliferation of tumor cells and enhancing the body immunity function; the lactalbumin, the hydrolyzed lactalbumin and milk powder provide high-quality proteins, and EPA and DHA contained in the deep sea fish oil also have an effect of inhibiting the proliferation of tumor cells, therefore, the nutrient meal disclosed by the invention is a total nutrient meal which is comprehensive and balanced in nutrition, and has a certain effect of enhancing the body immunity function of patients suffering from tumor and inhibiting the proliferation of tumor cells.

A method for preparing primary cell dental pulp stem construction and method of dental pulp stem cell library, the use of thermostatic shaker at specific temperature and speed conditions, can be in a few minutes after digestion, to extract enough has good biological activity of dental pulp stem cells, which can shorten the primary dental pulp stem cells generation of extraction time, save time and cost, large scale production can be obtained at the same time, plenty of pulp stem cells, can meet the requirements of dental pulp stem cells in stem cell library and later stem cells for clinical treatment and regenerative medicine research needs, and the pulp tissue under conventional semi digested 30min or full digestion 1H control group compared to the amount of DPSCs had no significant difference, but the cell proliferation activity was significantly higher than the control group. Therefore, this method can be carried out quickly in batches in the construction of DPSCs library, which shortens the time of DPSCs primary extraction, saves time and cost, and is suitable for large-scale production.

The invention relates to a celery fermented solution, a celery fermented beverage and preparation methods of products and belongs to the technical field of foods and biology. The celery fermented solution is obtained by carrying out enzymolysis on celery and carrying out combined fermentation by adopting various beneficial bacteria including lactobacillus acidophilus, lactobacillus plantarum, bacillus subtilis and the like. The celery fermented solution can be used as a raw material to prepare an enzyme beverage, such as the celery fermented beverage. The celery enzymatic hydrolysatefermented solution can be prepared by a method comprising the following steps: mixing 7 to 8 parts of the celery and 2 to 3 parts of water in parts by mass; crushing a mixture until the average grain diameter is 1mm to 3mm to obtain celery pulp; adding 0.05 percent to 0.2 percent of 10000U/g cellulase and 0.05 percent to 0.2 percent of 20000U/g to 50000U/g pectinase according to the mass of the celery pulp; carrying out the enzymolysis for 1h to 5h under the condition that the temperature is 40 DEG C to 50 DEG C, so as to obtain the celery enzymatic hydrolysatefermented solution. The product of the invention contains a large quantity of water-soluble dietary fiber components and functional oligosaccharide and has a bidirectional regulation effect of improving constipation and reducing diarrhea.

The invention discloses an anti-aging cosmetic composition. The composition comprises the following components in parts by weight: 1-15 parts of plant extract, 2.55-7 parts of emulsifier, 4-26 parts of emollient, 2.5-20 parts of humectant, 0.1-0.3 part of dipotassium glycyrrhizinate, 0.1-2 parts of silica, 0.03-0.07 part of potassium hydroxide, 0.1-0.2 part of thickening agent, 0.05-2 parts of antioxidant, 0.201-1.01 parts of preservative and 40-75 parts of water, wherein the plant extract is mixed liquid of a liquorice extract and an American ginseng extract. According to the anti-aging cosmetic composition, the mixed liquid of liquorice extract and American ginseng extract is applied to the anti-aging cosmetic, the proliferation activity of skin cells can be effectively improved, and the symptoms of skin aging can be reduced.

The present invention relates to follicular stem cell originated from human hair and its amplifying preparation process, and belongs to the field of medical preparation and human cell culture technology. The present invention adopts improved two-step protease digesting method to amplify follicular stem cell originated from human hair and obtains follicular stem cell with active expression of beta-integrin and keratin 19 in an optimized follicular stem cell preparing environment. The optimized micro environment endows follicular stem cell with fast amplification, antisenile characteristic, the epidermic cell-like, fibroblst-like and hair bulb tissue-like structure or functional expression. The present invention makes it possible to provide sufficient seed cell sources for the fast repair and functional reconstruction of skin defect.

The invention provides a fermentation product compounded plant extract anti-aging repair composition, application thereof and an essence emulsion containing the composition. The anti-aging repair composition comprises a fermentation product extract and a plant extract, wherein the fermentation product extract comprises 20-120 parts of a yeast/grape fermentation product extract, 45-180 parts of a split yeast fermentation product filtrate/lysate/extract and 30-160 parts of an alteromonas fermentation product extract; the plant extract comprises 2-20 parts of a perilla leaf extract, 3-16 parts ofa lantana camara leaf extract, 2-14 parts of bidens pilosa extract, 5-40 parts of an aesculus hippocastanum extract, 5-20 parts of a thyme extract, 3-36 parts of an aloe vera extract, 3-34 parts of acentella asiatica extract and 2-12 parts of a silybum marianum extract. The yeast extract composition disclosed by the invention is compounded with the plant extract composition with high oxidation resistance, so that rich active nutrition is provided for the skin, the injury is repaired, the premature senility is twisted, and the composition has an extremely high wound healing effect.

The invention relates to a preparation method of FGF1 sericin protein gel with activity for promoting cell proliferation and a product thereof. The preparation method comprises the following steps that sericin protein containing human FGF1 protein is extracted by human FGF1 gene modifying silk, and a sericin protein solution containing human FGF1 protein is obtained; after renaturation, an FGF1 sericin protein aqueous solution is obtained after dialysis with water, and FGF1 sericin protein hydrogel is obtained by centrifugation; and the FGF1 sericin protein hydrogel is placed at 4 DEG C, an FGF1 sericin protein gel material is formed by cross-linking of a chemical cross-linking agent or inducement of an inducer. The prepared gel material has the activity of promoting cell proliferation andhas broad application potential in tissue engineering.

The invention discloses an oral donkey-hide gelatin preparation and a preparation method thereof, and belongs to the technical field of production of traditional Chinese medicines. The oral donkey-hide gelatin preparation comprises ultrafine donkey-hide gelatin powder, rock sugar, soybean oil, tea oil, yellow rice wine, Jerusalem artichoke, fish bone meal, Radix Astragali, wolfberry fruits, chitosan and a slow release assistant. The preparation method comprises the following steps: preparing a dissolved gelatin solution, blending the dissolved gelatin solution, extracting effective traditionalChinese medicinal health components from the Jerusalem artichoke, Radix Astragali and wolfberry fruits, adding the chitosan to ensure the clearness of the preparation, adding the fish bone meal to supplement calcium element, and adding the slow release assistant to improve the absorption rate of nutrients in the oral donkey-hide gelatin preparation by using the drug effect slow release effect ofthe slow release assistant. The oral donkey-hide gelatin preparation prepared in the invention has an excellent drug absorption efficiency.

The invention relates to the technical field of medicine chemistry, in particular to a heteroaromatic ring compound with a thiosemicarbazone structure unit and a preparation method and application ofthe compound. The heteroaromatic ring compound with the thiosemicarbazone structure unit is chelated with metal ions in gastric cancer cells to form a stable complex, and accordingly the proliferationactivity of the gastric cancer cells MGC803 is inhibited. It is proved through a result of an embodiment that compared with a compound 3-AP, the heteroaromatic ring compound with the thiosemicarbazone structure unit has good proliferation inhibition activity on the gastric cancer cells MGC803.

The invention provides a method for induced differentiation of adipose tissue-derived stromal cells into liver-like cells. The method comprises the following steps: washing and cleaning adipose tissue, and adding isovolumetric tissue digestive enzyme for digestion; after completion of digestion, adding a trypsin solution for digestion; after completion of digestion, carrying out centrifuging so asto remove upper-layer grease and a digestive fluid, wherein precipitates are cells of the stromal vascular fraction of the adipose tissue; resuspending the cells of the stromal vascular fraction withphysiological saline, carrying out sieving so as to remove large tissues, carrying out washing, carrying out centrifuging so as to remove a supernatant, and after washing is completed, subjecting stromal vascular cells to inoculated culture so as to obtain the adipose tissue-derived stromal cells; selecting P3-generation adipose tissue-derived stromal cells for digestion so as to obtain a single-cell suspension, and carrying out inoculated culture with a culture medium overnight; removing a serum-free proliferation culture medium, and adding a first induction culture solution for induced culture; and replacing the first induction culture solution with a second induction culture solution for induction so as to obtain differentiated liver-like cells. The method provided by the invention canobtain functional liver cells by induction.

The invention provides gel jelly for improving joint health, and relates to the technical field of food processing. The gel jelly is composed of the following raw materials in parts by weight: 60-80 parts of purified water, 0.1-20 parts of enzymatic bone powder, 1-8 parts of collagen powder, 0.1-6 parts of chitosan oligosaccharide, 0.1-3 parts of gel powder, 2-10 parts of inulin, 0-3 parts of fructo-oligosaccharide, 1-10 parts of xylooligosaccharide and 0-5 parts of fructose. The invention has the beneficial effects that the gel jelly is prepared from the enzymatic bone powder, the collagen powder, the chitosan oligosaccharide and prebiotics as main raw materials, and the prepared gel jelly product is smooth in mouth feel and high in elasticity, has the function of improving joint health,also has the effects of regulating intestines, removing heavy metals and enhancing immunity, is low in calories and easy to eat, and is novel health-care gel jelly.