Method for induced differentiation of adipose tissue-derived stromal cells into liver-like cells
A kind of mesenchymal stem cells and cell technology, applied in the field of induction and differentiation of adipose-derived mesenchymal stem cells into liver-like cells, can solve the problems of different ratio combinations of cytokines, influence on induction efficiency, cross-contamination, etc., and achieve high cell proliferation activity and high induction efficiency , The effect of easy material collection
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[0028] The technical solutions of the present invention will be described in further detail below in conjunction with specific examples, but the present invention is not limited to the following examples.
[0029] To induce the differentiation of adipose-derived mesenchymal stem cells into liver-like cells, the steps are as follows:
[0030] 1. 30ml of adipose tissue comes from the abdomen of a healthy adult donor who underwent liposuction to lose weight, and signed an informed consent;
[0031] 2. After being collected in the surgical operating room of the plastic surgery hospital, it is sealed in a transport bottle for refrigerated transportation (4-8°C). 50ml of tissue protection solution needs to be added to the transport bottle. Prepared with 5μg / ml amphotericin B to ensure no bacterial and fungal contamination during transportation;
[0032] 3. After the adipose tissue arrives in the laboratory, clean the tissue cleaning solution (the tissue cleaning solution is prepare...
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