353results about How to "Improve induction efficiency" patented technology

The invention discloses an induction method of a three-dimensional (3D) online induction system for mechanical equipment dismounting. The induction system comprise an eyeglass-type augmented reality helmet, a hand position tracking device and a computer, wherein the augmented reality helmet is provided with two display screens and two cameras; and wireless communication can be realized among the augmented reality helmet, the hand position tracking device and the computer. An induction method for realizing the three-dimensional online induction system for mechanical equipment dismounting comprises the following steps of: 1, transforming a virtual assembly model; 2, initializing the induction system; 3, recognizing the dismounting purpose; 4, generating induction information; 5, overlapping the induction information; and 6, repeating the steps 3-5 until the online product induction is finished. The method disclosed by the invention has the characteristics of accurate induction information, strong universal capability and convenience in carrying, and can be used for realizing the 3D online induction driven by requirements of operators, current situations of tasks and dismounting history and realizing the integration of product 3D design and dismounting induction.

The invention discloses a rotary ultrasonic head based on machine tool attachment. The rotary ultrasonic head comprises an ultrasonic vibration operating device, a non-contact collector ring, a main shaft, a knife handle and a supporting shell, wherein, the knife handle with a standard model is adopted; the ultrasonic vibration operating device is connected with one end of the main shaft; the knife handle is connected with the other end of the main shaft; rotation axes of the ultrasonic vibration operating device, the knife handle and the main shaft are positioned on the same straight line; and the non-contact collector ring is arranged between the main shaft and the supporting shell, and an ultrasonic vibration signal is transmitted to the ultrasonic vibration operating device in a non-contact manner through the non-contact collector ring. The rotary ultrasonic head has the following outstanding advantages: (1) complete machine tool attachment as well as generality and interchangeability of an ultrasonic machining device are realized; and (2) the induction efficiency of a brushless non-contact power supply system of the ultrasonic machining device is improved, the efficient energy transfer is realized, and the energy loss is reduced.

The invention provides a cell preparation for treating osteoarthritis and a preparation method thereof. The cell preparation mainly comprises mesenchymal stem cells, differentiation-induced stem cells, platelet-rich plasma and dispersion media thereof. Compared with the prior art, the cell preparation for treating osteoarthritis has the advantages that the mesenchymal stem cells are mixed with the differentiation-induced stem cells and compounded with the platelet-rich plasma and can be dispersed in the joint cavity, so that the immune regulation function of the mesenchymal stem cells can be exerted, the microenvironment of the whole joint cavity can be improved, and inflammation remission and injury repair are facilitated; tissues can be repaired after the differentiated mesenchymal stem cells are implanted into the body, so that the repair process is shortened.

The invention discloses a method for producing stem cell factors by inducing human mesenchymal stem cells. The invention also discloses a method for preparing freeze-drying powder of a human mesenchymal stem cells culture supernatant and prepared freeze-drying powder. The method has the advantages of high induction efficiency and large output of stem cell factor, and can obtain a lot of stem cell factors in short time. The method of for preparing freeze-drying powder of the human mesenchymal stem cells culture supernatant is simple and convenient, compared with a low temperature induction method, the output is increased by 15%-20%; the method for preparing freeze-drying powder of the human mesenchymal stem cells culture supernatant preserves various cytokine mixtures having biological activity in the human mesenchymal stem cells culture supernatant, and establishes a base for application of the human mesenchymal stem cells culture supernatant on beauty treatment aspect.

The invention relates to a parking space state sensing and indicating device and a parking space state sensing and indicating method. The parking space sensing and indicating device comprises an infrared distance detecting module, a high-light LED indicating module, a communicating module and a control module, wherein the infrared distance detecting module is used for continuously detecting the distance between a mounting position of the parking space state sensing and indicating device and a parking space ground surface or the top portion of an object placed on a parking space according to a specific time interval; the high-light LED indicating module is used for indicating parking space state information, when the parking space is vacant, an LED flashes according to a certain time interval, and when the parking space is occupied, the LED extinguishes; the communicating module is used for sending parking space state information outside; the control module is used for judging whether a vehicle is parked on the parking space according to distance changes detected by the infrared distance detecting module and used for driving the high-light LED indicating module to indicate the parking space state information and driving the communicating module to send out the parking space state information. The parking space state detecting module and the parking space state indicating module are integrated in the parking space state sensing and indicating device, and therefore mounting efficiency is improved and system cost is lowered.

A system and method are provided to increase the horsepower of an induction motor. One technique for increasing the horsepower of a motor is to connect the motor to a variable speed drive that is providing an output voltage and frequency greater than the standard line voltage and frequency. The connection of the variable speed drive to the induction motor enables the motor to be operated in constant flux or constant volts / Hz mode and provide a greater output horsepower. Another technique for increasing the horsepower of a motor is to use a dual voltage motor configured for lower voltage operation and then provide the motor with the corresponding voltage and frequency for higher voltage operation. The higher voltage and higher frequency can be provided by a variable speed drive with or without voltage boost. A variable speed drive without voltage boost, but with frequency boost, can be used in situations where the standard main voltage is greater than the lower voltage rating.

The efficiency of an induction motor is improved while suppressing the generation of magnetic flux saturation of a rotor core. In an induction motor, “a magnetic path width of a rotor” which is the product of a circumferential width of a rotor tooth formed in the rotor and the number of rotor teeth is equal to or larger than “a magnetic path width of a stator” which is the product of a circumferential width of a stator tooth formed in the stator and the number of stator teeth.

The invention provides an inducing culture method for regulatory T cells and relates to an inducing culture method for regulatory gamma delta T cells. In the method, cell factors are applied and deoxyribonucleic acid (DNA) demethylated medicament decitabine is combined so as to cooperatively induce the generation of the regulatory gamma delta T cells, and an enrichment process of magnetic cell sorter (MACS) positive sorting is adopted; after enrichment, detections of cell growth state and activity are carried out so as to ensure the next experience. The method has the advantages of simple and practicable inducing and enrichment processes, short period, low cost, high inducing efficiency and good repeatability; and after enrichment, cell activity is good, the quantity and activity of cells can ensure the next in vivo and in vitro studies, thereby providing a good study platform for defining the biological characteristics of the gamma delta T cells. Thus, the method has a good popularization value.

The invention discloses a method for inducing a polyploid of momordica grosvenori. The method comprises the steps of dipping a pollinated momordica grosvenori female flower by using Oryzalin; and then washing and relieving the dipped flower; and inducing doubling of zygotes by using a novel chemical inducer Oryzalin so as to obtain the polyploid. According to the invention, the chimera problem of the polyploid of the momordica grosvenori is solved, the inducing operation procedures are simplified, the inducing efficiency of the polyploid of the momordica grosvenori is increased, and convenience is obtained in actual applications.

The invention discloses a cultivating method of the isolated microspores of brassica napus, which aims to solve the drawback of embryo index unavailable in prior art that, the production of microspore embryos can not be judged in the early stage. The invention is characterized in that, a whole filtered and sterilized NLN-13 hormone-free liquid culture medium comprising 45 to 55 mg / L colchicine is added to the prepared isolated microspores of brassica napus; the microspores are cultured in dark under a high temperature raging from 30 deg C to 35 deg C for two days; the materials having the microspore swell ratio large than 50% are chosen for subsequent induction culture. The cultivating method has the advantages that, the microspores having the microspore swell ratio large than 50% are chosen for subsequent induction culture, so the early rejection of the microspores that cannot produce embryos is facilitated; the workload is greatly reduced; the induction efficiency is improved, and the waste of time and resources is avoided.

The invention provides a culture method of rapamycin induced regulatory gamma delta T cells. The culture method is characterized in that mononuclear cells derived from human peripheral blood are amplified by uniting multiple cell factors with macrolides immunosuppressor-rapamycin through induction in vitro, so that the lots of regulatory gamma delta T cells are cultured. The culture method is simple in the whole process of induction, amplification and enrichment, short in cell culture period and low in cost, has strong feasibility, high induction efficiency and good repeatability and can be applied to the culture of the regulatory gamma delta T cells. The regulatory gamma delta T cells induced by the method have good activity, a relatively strong immunosuppression function and very high popularization value and can be used for ensuring the further research.

The invention discloses a medium for inducing human umbilical cord mesenchymal stem cells to differentiate into islet-like cells and an inducing method thereof. The medium contains activin A, epidermal growth factor, β-nerve growth factor, nicotinamide, 1% insulin-transferrin-selenium, and 10 μg / L basic fibroblast growth factor UltraCULTURE medium. The induction method is as follows: the mesenchymal stem cells are induced, differentiated and cultured using the above-mentioned induction medium. The induction medium of the invention contains simple induction factors, does not contain toxic and harmful substances, and is safer. The method of the present invention for inducing human umbilical cord mesenchymal stem cells to differentiate into islet-like cells can obtain aggregated islet-like cells in 21 days, and partially mature and differentiated islet-like cells can be obtained in 28 days. A standardized and novel induction method has been established. Broad clinical application prospects.

The invention provides a medium for inducing pluripotent stem cells. The medium is an LBX liquid medium, and the stem cells are porcine stem cells, mouse stem cells, monkey stem cells or human stem cells. The invention also provides a method for inducing the pluripotent stem cells by using the medium. The medium for well improving the induction efficiency of induction pluripotency is invented for the first time. The cost of the medium is low. Induced pluripotent stem cells induced in the invention are more likely to be in a mouse-like state, and single cell passage can be realized, so large-scale amplification and clinic application are benefited.

The invention discloses an efficient genetic-modification-free iPSC induced and industrialized monoclonal picking platform and application. The platform can efficiently perform reprogramming and only needs to use a minimum number of reprogramming factors (OCT4, SOX2, E6 and E7). In a monoclonal separation stage, SSEA4 / TRA-1-60 is taken as a selection marker, a large number of single cell clones are obtained through a flow cytometry sorting technology. The platform has the advantages of being high in reprogramming efficiency, high in safety, easy to operate, capable of achieving large-scale production and the like.

The invention relates to a method for improving tissue culture reproductive speed of Alpinia zerumbet, which relates to the tissue culture technology of Alpinia zerumbet, and comprises the following steps that: A. seed collection is proceeded after 7 to 8 continuous sunny days, clumped Alpinia zerumbet plants which are used as seed are dug out from the soil 2 to 3 days before the seed production, soil is removed, and the Alpinia zerumbet plants are hung upside down to be dried for 2 to 3 days at a cool well-ventilated indoor place; B. lateral buds on tubers or spouts are selected as explant materials; C. adventitious buds are inducted to produce; D. adventitious buds are bred and cultured; E. adventitious buds are rooted to culture; and F. seedlings are transplanted. The rapid formation of adventitious buds is promoted through high temperature and high-concentration hormone, so the induction efficiency is improved, and the culture time is shortened; the formation of variegated leaf solid buds can be realized by changing the culture medium formula, reducing the culture temperature and increasing the light density, whitening variation can be reduced, the rapid breeding rate and seedling formation rate can be effectively improved, the factory-oriented production progress of the tissue cultured seedling can be accelerated, and the production cost can be reduced.

The invention discloses a mutual shielding type electric field sensor based on torsional vibration. The mutual shielding type electric field sensor comprises a substrate, an electric field sensing structure, torsional elastic beams connected with movable torsional electrodes and a driving structure, wherein the electric field sensing structure comprises at least one group of fixed electrodes and movable torsional electrodes, the fixed electrodes and the movable torsional electrodes are sensing electrodes and are mutual shielding electrodes, namely the fixed electrodes and the movable torsionalelectrodes produce relative movements and are mutually shielded when the movable torsional electrodes perform torsional vibration, and meanwhile the fixed electrodes and the movable torsional electrodes produce sensing charge change. The driving structure drives the movable torsional electrodes to perform torsional vibration around the torsional elastic beams, so that relative position change isproduced between the fixed electrodes and the movable torsional electrodes. The electric field sensor is simple in structure, small in size, high in sensing efficiency, wide in use range, suitable forcommon machining and micromachining technology preparation, easy to assemble and integrate and suitable for batched-type and large-scale production.

The invention discloses a flounder tetraplont fry batch induction method comprising an induction method and an identification method of tetraplont fries; wherein, the induction method comprises collection and insemination of unfertilized ovum, and determination of oosperm chromosome doubling starting time, hydrostatic pressure shock pressure and processing time, and the identification method comprises ploidy determinator identification and chromosome analysis identification. The method firstly builds a method of inducing the tetraplont fries of flounder, such as paralichthysolivaceus and the like by hydrostatic pressure, screens hydrostatic pressure processing starting time and hydrostatic pressure shock conditions for inhibiting first merogenesis and carrying out chromosome doubling, and builds a tetraplont fry batch induction method. Tetraplont fries obtained with the method have high induction rate of 90%; the method of the invention has the characteristics of advancing, high efficiency, accuracy and reliability, and has important application value in fish polyploidy induction and sterile offspring seed production, and has wide promotion and application prospect in fish cultivation and breeding.

The invention discloses a culture medium and a propagation method for tissue culture rapid propagation of leaves of heuchera micrantha. Through selection on an explants, selection on a basic culture medium and hormone combination in proper dosage, high-effective regeneration of a plant of the heuchera micrantha during the tissue culture is achieved. The propagation method is simple operation and low pollution rate, is high in success rate of regeneration of the plant, and is short in production period of grown-up seedlings.

The invention discloses an in vitro induction amplification, freeze preservation and anabiosis method of immune cells. The method comprises the following steps: using a CD16 antibody enveloped culture container to obtain an enveloped culture container; using an immune cell activated culture medium to put single karyocytes into the enveloped culture container to carry out first induction and amplification culture so as to obtain primarily induced and amplified immune cells; using an immune cell amplification culture medium to carry out second induction and amplification culture on the primarily induced and amplified immune cells so as to obtain differentiated immune cells; using an immune cell large-scale amplification culture medium to carry out third induction and amplification culture on the differentiated immune cells so as to obtain large-scale function activated immune cells; using an immune cell freeze preservation liquid to freeze and preserve the immune cells so as to obtain frozen and preserved immune cells; water-bathing the frozen and preserved immune cells for melting, mixing with a freezing anabiosis liquid of the immune cells so as to obtain an anabiosis cell mixed liquid; carrying out centrifugal treatment on the anabiosis cell mixed liquid so as to obtain anabiosis immune cells.

The invention provides a culture medium for culturing induced pluripotent stem cells. The culture medium contains a ginsenoside monomer Rb1, a ginsenoside monomer Rb3 and / or a ginsenoside monomer Rd. The invention also provides a method for inducing pluripotent stem cells. The method comprises the following steps: (1) introducing pluripotent-related genes into donor cells; (2) preparing the donor cells in which the pluripotent-related genes are introduced into single-cell suspension, and culturing the donor cells (in which the pluripotent-related genes are introduced in the step (1)) by using the culture medium provided by the invention; and (3) identifying cloning of the pluripotent stem cells. In consideration of the application bottleneck of ips to clinical test at present, the ginsenoside monomer Rb1, the ginsenoside monomer Rb3 and the ginsenoside monomer Rd are discovered, the ips induction efficiency can be obviously improved, cell senescence can be obviously delayed, cell proliferation can be promoted, and far-reaching influence is generated in the entire field of stem cell research and new drug research and development.

The invention discloses a non-contact power supply device for a rotating mechanism, which comprises a primary side and a secondary side. The primary side consists of a primary side coil, and the secondary side consists of a secondary side core and a secondary side coil wound on the secondary side core. The primary side coil is arranged statically relative to a base of the rotating mechanism. The secondary side core is fixedly arranged on a rotating body of the rotating mechanism, and rotates along with the rotating body. The primary side coil is connected with a power supply arranged outside the rotating mechanism, and electrical energy is transferred to the primary side coil of the non-contact power supply device. The primary side coil transfers the electrical energy to the secondary side coil of the non-contact power supply device in a non-contact way by electromagnetic induction. The secondary side coil of the non-contact power supply device is connected with a part required to be supplied with power on the rotating body of the rotating mechanism, and finally the electrical energy is transferred to the part required to be supplied with the power on the rotating body. The non-contact power supply device provided by the invention realizes the highly-efficient energy transfer of the non-contact power supply device of the rotating mechanism, reduces energy loss and avoids limitations to rotating speed.

The invention discloses a high-efficiency method for the induction and the successive transfer proliferation regeneration of banana embryogenic calluses. The technological process comprises a culture process of inducing dedifferentiated calluses, a culture process of inducing fragile embryogenic calluses, a successive transfer proliferation process of the fragile embryogenic calluses, an inducing and embryo-forming process of the embryogenic calluses and an inducing and seedling process of mature embryos. The invention has the advantages of simple technological process, low production cost, high embryogenic tissue induction and proliferation rate and long successive transfer time, solves the problems of low embryonic reaction of an exophyte, hard induction of the embryogenic calluses, low induction embryo-forming and seedling rates, long induction period, and the like in the culture process of banana tissues, provides a new technological route for a banana transform technology and provides a high-efficiency regeneration system for producing juvenal tissue and culturing banana seedlings, thereby having a great application value.

The invention discloses a method of extracorporeal induction, proliferation and cryopreservation of immune cells. The method comprises the steps of utilizing a CD16 antibody to cover a culture vessel to obtain the culture vessel after covering; utilizing the immune cells to activate a culture medium, and placing single cell nucleus in the culture vessel after covering to activate the culture medium through the immune cells to obtain preliminarily induced and proliferated immune cells; utilizing a immune cell proliferation culture medium to conduct second induction and proliferation culture on the preliminarily induced and proliferated immune cells to obtain differentiated immune cells; utilizing immune cell scale proliferation culture to conduct third induction proliferation culture on the differentiated immune cells to obtain large-scale immune cells with activated functions; utilizing immune cell cryopreservation liquid to cryopreserve the large-scale immune cells with the activated functions. The method of extracorporeal induction, proliferation and cryopreservation of the immune cells has the advantages of being high in induction efficiency, high in proliferation speed, high in safety, low in cost and the like. Moreover, the cryopreserved large-scale immune cells have long effective storing time and high cell recovery rate.

The invention discloses an induction method of poplar tetraploid plants, belonging to the field of plant inheritance breeding. The method of the invention comprises the following steps: pollination is carried out on female inflorescence of poplar female parent by powder of poplar male parent to form infructescence, when flocculent fiber is formed and wraps ovule in the infructescence ovary, physicochemical treatment is carried out on the infructescence, seed selection is carried out on tetraploid plants; the inventor of the invention discovers by tests that the effective period for carrying out physicochemical treatment to induce the zygote chromosome to be reduplicated is when most zygotes of the poplar is at the first mitosis process and the flocculent fiber starts to wrap but does not completely covet the ovule, thus the induction efficiency of tetraploid plants is improved. The invention avoids verbose links such as fixing, dehydrating, waxing, embedding, slicing, pigmentation, microexamination of the ovule and the like, thus enhancing the timeliness of physicochemical treatment, saving induction reagent and experimental material and improving the precision and stability of the reduplication of zygote chromosome.

The invention relates to an in vitro culture kit for T-lymphocyte cells, which fundamentally solves the problems of existing T-lymphocyte cell in vitro culture methods that the used materials and culture time are inconsistent, and the culture periods, quality and quantity of effective cells are different. The technical key points are as follows: the content comprises a coating bottle for inducting the differentiation of the T-lymphocyte cells; a CD3 monoclonal antibody, gamma- interferon and interleukin-1 alpha are pre-coated in the coating bottle; the component of a cell factor A, a cell factor B and a cell factor A for stimulating the division and proliferation of the T-lymphocyte cells is 200000 units of interleukin-2, and the component of the cell factor B is 2 million units of interleukin-2. According to the in vitro culture kit for the T-lymphocyte cells, the composite reagent and materials required for the in vitro culture of the T-lymphocyte cells are combined together in a standard way, all the operation is standardized and modularized, so that not only is the working efficiency improved, but also the cell quality and yield difference caused by the operation of different people is reduced.

The invention relates to the biotechnical field, and concretely relates to a medicinal composition for inducing direct conversion of fibroblasts into nerve cells, and a use of the medicinal composition. Transdifferentiation between nerve cell non-genealogies is realized through micro-molecular compound combination without exogenous gene. VCRFSGY treated fibroblasts rapidly leave the cell cycle at the third day is found for the first time, which hints that neuron cells formed by chemical induction of human fibroblasts may bypass the proliferation intermediate state, so the medicinal composition has extremely high clinic and market values. Additionally, a situation that Retinoic acid or Parnate can be respectively added on the basis of a VCRFSGY compound combination in order to well promote the induction efficiency is further found.

The invention provides a method for in-vitro induction of a lobed kudzuvine root microtuber, and relates to the technical field of lobed kudzuvine root cultivation. The method comprises the followingsteps that 1) a rooting culture medium is inoculated with lobed kudzuvine root tissue culture seedlings for rooting culture so as to obtain rooted seedlings; and 2) a microtuber induction culture medium is inoculated with the rooted seedlings for induction culture so as to obtain the lobed kudzuvine root microtuber. The method has the advantages that the induction rate of the microtuber can be obviously increased, the induction time of the microtuber can be shortened, and the induction efficiency of the microtuber can reach 95% or above within the induction time of 20-25 days.

The invention relates to the technical field of regenerative medicine and discloses a method for adjusting and controlling in vitro three-dimensional directed differentiation of stem cells. The method includes following steps: embedding Stem cells into calcium alginate micro gel beads containing chondroitin sulfate, adding an inductive differentiation culture liquid, adjusting and controlling the in vitro directed differentiation of the stem cells by changing a mass ratio between the sodium alginate and the chondroitin sulfate during the preparation of the micro gel beads, dissolving the calcium alginate micro gel beads in a sodium citrate solution after the differentiation process being finished, and then obtaining pure differentiation cells. The method is simple in operation and low in cost, the calcium alginate micro gel beads containing chondroitin sulfate can simulate an in vivo three-dimensional extracellular matrix so that a three-dimensional directed differentiating microenvironment which approximates an in vivo microenvironment is supplied for the stem cells, thereby improving directed differentiation efficiency and a biological function of the stem cells. The method plays an important role in applications of regenerative medicine.

The invention introduces an effective inductive cultivation method for an autotetraploid of paulownia catalpifolia by using leaves of a diplontic paulownia catalpifolia tissue culture seedling. The method comprises: pre-cultivating the leaves of paulownia catalpifolia for 6 days, wherein the concentration of added colchicine is 30 mg / L, the concentration of dimethyl sulfoxide is 2%, and the soaking time is 48 hours; performing shake cultivation, wherein the induction rate of the tetraploid paulownia catalpifolia reaches 14.5%; detecting that the chromosome number of root tip cells of variant plants of paulownia catalpifolia is 80 by virtue of a chromosome counting method, wherein the chromosome number is two times of that of the corresponding diplontic paulownia catalpifolia; and detecting that the unicellular DNA content of the tetraploid leaves of the paulownia catalpifolia are two times of those of the corresponding diplontic paulownia catalpifolia by using a flow cytometer, showing that a tetraploid paulownia catalpifolia plant is obtained. The cultivation method for inducing autotetraploid of paulownia catalpifolia by colchicine disclosed by the invention has the advantages of being wide in material drawing, simple and convenient to operate, low in cost, high in induction rate and the like, and is of certain guiding significance for woody plants, the tetraploid of which is not easily obtained.