173results about How to "Improve proliferation efficiency" patented technology

The present invention relates to a fast reactor type coupling nuclear reaction implementation method and a nuclear reactor for same. The main contents comprise: a fast reactor type coupling nuclear reaction implementation method, a reactor modular design approach, a fast reactor type coupling nuclear reactor, a reactor core, a fuel element, a nuclear control system, and a proliferation fuel system. The fast reactor type coupling nuclear reactor mainly combusts thorium and nuclear waste, and has inherent security. The reactor main container is composed of a fission pool and a moderating pool that are completely isolated from each other but coupling to each other. A primary coolant is separated from a moderator. A thermal insulation layer is disposed between the fission pool and the moderating pool so that both can perform neutron exchange but heat exchange is blocked. Fast neutrons produced by the fission pool and moderated neutrons reflected by the moderating pool may enable the reactor core to simultaneously perform coupling nuclear reaction of the two types of neutrons. The moderating pool may be provided with the nuclear control system, and ex-core coupling core control may be implemented. The moderating pool is provided with a thorium purification fuel system, and on-line extraction of the purification fuel can be performed, and separation of nuclide is safe and simple, thereby providing a solution to the technical bottleneck of "thorium reactor".

The invention belongs to the technical field of the nuclear reactor engineering, particularly to the water-cooling two-region multiplication nuclear reactor core and the nuclear reactor adopting the same, wherein the water-cooling two-region multiplication nuclear reactor core includes a fast neutron energy spectrum area adopting the coating grain sheath fuel assembly and a thermal neutron energy spectrum area adopting the dense rod bundle fuel assembly or the coating grain sheath fuel assembly. The water-cooling two-region multiplication nuclear reactor core of the invention adopts the coating grain sheath fuel assembly, which breaks through the restrain of the stainless steel shell to the outlet scream temperature, realizes the higher coolant outlet temperature, meanwhile the fast neutron core is leaked to the thermal neutron energy spectrum core constituted by the rod bundle fuel assembly dense boom at the subcritical to be multiplied.

The invention discloses an efficient multiplication CTL preparation method killing tumors in a targeted mode. The CTL preparation method comprises the following steps: (a) removing CD4+CD25+Treg cells through immunomagnetic bead negative sorting; (b) arranging mixed cells in a serum-free medium for cultivation, and obtaining suspension cells and adherent cells; (c) adding GM-SCF and IL-4 in the adherent cells, culturing the cells for five days; in the sixth day, adding a tumour cell holoantigen, and in the seventh day, adding TNF-alpha and IL-27; (d) transferring the suspension cells to a culture flask wrapped by a CD3 monoclonal antibody and recombinant human fibronectin, adding IFN-gamma, in the second day, adding IL-2, IL-12 and the IL-27, and culturing the mixture till the eighth day to obtain CIK cells; (e) mixing the CIK cells and mature DC cells, and adding the IL-12, IL-7 and an anti-CD 28 monoclonal antibody for cultivation; in the third day, adding an anti-CTLA-4 monoclonal antibody, and then culturing the mixture for four days. According to the efficient multiplication CTL preparation method killing tumors in the targeted mode, efficiency of in-vitro CTL cell proliferation is improved, activity of killing the tumor cells in the targeted mode is improved, transformation of peripheral blood mononuclear cells to the CD4+CD25+Treg cells is inhibited.

The invention discloses a method and application for inducing human umbilical cord mesenchyme stem cells to be differentiated into testicular interstitial cells. The method comprises the following step of culturing human umbilical cord mesenchyme stem cells of patients suffering from adenovirus and carrying mice steroidogenic factor-1 genes in a DMEM-F12 culture solution containing 0.3-3ng / ml of luteinizing hormone, 200-800mu M of dibutyryl cyclic adenosine monophosphate, 5*10<-6>-5*10<-4>M of all-trans retinoic acid (ATRA), 10mU / ml of human chorionic gonadotropin and 2.4uM of adrenocorticotrophic hormone for a week. Induced by the method in the invention, the human umbilical cord mesenchyme stem cells can be differentiated into testicular interstitial cells in vitro and provides important sources of cells for treating testosterone shortage by the cell replacing method or the genetic method.

This invention relates to an instrument for regenerating a living organism tissue or organ, characterized in that a support (A) formed from a biodegradable material or a bioabsorbable material includes a sponge-like fine matrix (B) formed from a biodegradable material or a bioabsorbable material and a linear guide channel (C) for a living organism tissue or organ.

The invention discloses a method for inducing human umbilical cord mesenchymal stem cells in vitro into liver cells and application thereof. The method comprises the following steps of: at the culture logarithmic growth phase of human umbilical cord mesenchymal stem cells, adding a liver cell induced differentiation culture medium and performing differentiated induction to obtain the liver cells formed by the differentiation of the human umbilical cord mesenchymal stem cells. The liver cell induced differentiation culture medium consists of the basic culture medium is an IMDM culture medium containing 10 to 70 ng / ml of liver cell growth factor, 3 to 30 ng / ml of fibroblast growth factor, 4.5 to 50 ng / ml of oncostatin, 2 to 40 ng / ml of alkali fibroblast growth factor, 20 ng / ml of epidermal growth factor, 1 mu M of dexamethasone and 50 mg / ml of ITS+premix. The method has the characteristics of one step method, simplicity, high repeatability, short duration time of differentiation process and good induced differentiation effect. The method can be applied to the research on turning mesenchymal stem cells into liver cells by induced differentiation.

Cell aggregate formation control and cell aggregate disintegration control are carried out with respect to cells in a culture container to increase cell proliferation efficiency. The number of the cells in the culture container is counted without disassembly of a culture system and irrespective of the density of the cells.An external force is applied to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells in the culture container, thus culturing the cells. The external force is applied to the culture container by pressing an agitating member onto a top surface of the culture container placed in flat orientation to a predetermined pressing degree, and moving the agitating member in a horizontal direction at a predetermined speed, thus controlling at least one of cell aggregate formation and cell aggregate disintegration with respect to the cells in the culture container. A method for counting counted targets disposed in a liquid enclosed in a container includes adjusting a thickness of at least a part of the container. At least a part of the adjusted part is set as a measurement target region. A number of counted targets in the measurement target region is counted.

A method of tissue culture and rapid propagation of healthy sugarcane seedlings comprises: detoxifying a small amount of cleaned sugarcane mature seed rhizomes with warm of 52 DEG C water for 30 minutes, accelerating germination at 38 DEG C, using auxiliary buds as explants for induction, differentiation and breeding to obtain first generation healthy seedlings, detecting ratoon stunting diseasesby means of PCR (polymerase chain reaction) to detect plantlets with negative results, obtaining a great amount of healthy sugarcane seedlings by means of multiplication culture, performing rooting cultivation, seedling adaptation and transplanting to a sand bed for temporary planting, sampling the seedlings for detecting the ratoon stunting diseases by means of PCR, proving qualification of thehealthy sugarcane seedlings, and transplanting the qualified seedlings to a large land or popularizing planting of the seedlings. The method can improve production efficiency of healthy sugarcane seedlings, twice PCR detection guarantees detoxification effect of the healthy sugarcane seedlings, the whole production process is short, propagation by multiple nursery is not needed, seedlings from tissue cultivation are directly used for production or application, the method has the advantages of high efficiency, superior quality, fastness and the like and meets large-scale factory production requirements of the healthy sugarcane seedlings.

The invention discloses a micropropagation method of fraxinus rhynchophylla and relates to the micropropagation method. The problems of long reproductive cycle, low reproduction efficiency and poor offspring genetic stability in nursery stock propagation of fraxinus rhynchophylla are solved. The method comprises the following steps of: 1, sterilizing fraxinus rhynchophylla seeds, and culturing single cotyledons of zygotic embryos to obtain cotyledonal cell embryos; 2, performing maturation cultivation on the cotyledonal cell embryos; 3, performing sprouting cultivation on the cotyledonal cellembryos subjected to maturation cultivation to obtain regenerated plants; and 4, transplanting the regenerated plants into a culture medium to culture until new leaves are completely unfolded, and removing a covered plastic thin film. The micropropagation method has the advantages of short nursery stock reproductive cycle, high reproductive rate (culturing for 60 to 70 days) and high reproductionefficiency (each explant can generate 15 to 20 somatic embryos); the germination rate of the somatic embryos is up to 87 to 89.55 percent; the transplanting survival rate of the regenerated plants isup to 75 to 80 percent; and the regeneration plants with the same good characteristics as female parents can be generated in mass.

The invention provides a preparation method of ascites tumor cell sensitized DC-CIK and application of the DC-CIK. The preparation method of ascites tumor cell sensitized DC-CIK comprises steps of: preparation of ascites tumour antigen; preparation of cord blood mononuclear cells; separation of cord blood lymphocytes and adherent cells; induction and amplification of the CIK cells; induction and amplification of the DC cells and loading of the ascites tumour antigen by the DC cells; and preparation of DC-CIK through co-culture of DC cells and CIK cells. The DC-CIK prepared by the preparation method of ascites tumor cell sensitized DC-CIK of the invention has high proliferative activity and tumor killing activity. The method has simple operations, easily controlled conditions and low equipment requirements.

The invention discloses a high-efficiency method for the induction and the successive transfer proliferation regeneration of banana embryogenic calluses. The technological process comprises a culture process of inducing dedifferentiated calluses, a culture process of inducing fragile embryogenic calluses, a successive transfer proliferation process of the fragile embryogenic calluses, an inducing and embryo-forming process of the embryogenic calluses and an inducing and seedling process of mature embryos. The invention has the advantages of simple technological process, low production cost, high embryogenic tissue induction and proliferation rate and long successive transfer time, solves the problems of low embryonic reaction of an exophyte, hard induction of the embryogenic calluses, low induction embryo-forming and seedling rates, long induction period, and the like in the culture process of banana tissues, provides a new technological route for a banana transform technology and provides a high-efficiency regeneration system for producing juvenal tissue and culturing banana seedlings, thereby having a great application value.

The invention relates to asparagus parent breeding method, mainly solving the problems that rooting rate is low, root quality is poor and the survive rate of the regeneration plant is low in asparagus organ tissue culture process. The method includes the process that high quality asparagus stem section is selected, culture is carried out in culture medium solution, and asparagus seedling meeting transplant standard is bred. The culture medium solution is confected by the way that hormones corresponding to various culture stages are added on the basis of the basic culture medium solution, the culture includes induction culture, propagation culture, rooting pre-differentiation culture and rooting culture sequentially. Compared with the existing organ culture, the invention has high propagation rate, rooting rate reaches 100%, root system quality is good, and differentiation rate of strong fleshy tap root is improved to 96%, thus ensuring survival rate after transplantation.

The invention discloses a method for efficiently propagating dendrobium by using roots and a culture medium of the dendrobium. The method in the invention comprises the following steps of: selecting a root section of the dendrobium, and incubating and inducing the root section to obtain an embryonic callus, wherein the embryonic callus can be greatly multiplied through subculture; transferring the embryonic callus into a protocorm induction culture medium for induction and multiplication to obtain protocorm and bud seedlings; and transferring the protocorm and the bud seedlings into an adult seedling culture medium and culturing a dendrobium rooting plant. According to the method, MS is used as a basic culture medium; 6-BA and PIC are required to be added into the culture medium for the embryonic callus induction or subculture; CPPU and NAA are required to be added into the culture medium for the protocorm induction. The method for culturing regenerated plants by using the dendrobium roots provided by the invention is high in propagation coefficient, high in planting percentage and low in cost; a seedling growing process, a seedling robusting process and a rooting progress of the protocorm can be further finished; the plants are robust, and the roots are robust.

A set of Sinkiang drug mulberry tissue culture and rapid propagation culture medium comprises the following components: a germination culture medium, a multiplication culture medium, an extension culture medium and a root culture medium, wherein the germination culture medium is prepared from the following raw materials: MS 4.33 g / L, 6-BA 2.0 mg / L, NAA 0.2 mg / L, sugar 30 g / L and agar 7 g / L, and the pH value of the germination culture medium is 5.8; the multiplication culture medium is prepared from the following raw materials: MS 4.33 g / L, 6-BA 1.0 mg / L, sugar 30 g / L and agar 7 g / L, and the pH value of the multiplication culture medium is 5.8; the extension culture medium is prepared from the following raw materials: MS 4.33 g / L, ZT 4.5 micro-mol / L, sugar 30 g / L and agar 7 g / L, and the pH value of the extension culture medium is 5.8; and the root culture medium is prepared from the following raw materials: WPS 2.30 g / L, L+NAA 0.2 mg / L, IBA 0.2 mg / L, activated carbon 0.4 g / L, sugar 20 g / L and agar 6 g / L, and the pH value of the root culture medium is 6.0. The culture medium is simple and convenient to prepare, and is high in multiplication efficiency, each seedling can differentiate 2.3 lateral buds averagely, the transplanted seedlings are got by winter buds after four months, and the survival rate after transplantation is high to more than 75%.

Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same.A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g / cm3 to 0.90 g / cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g / cm3 to 0.93 g / cm3 and forming a cell culture surface. A cell culture container is formed by using this multilayer film.

The invention discloses a method for high-efficiency somatic embryogenesis and plant regeneration of camellia plants, comprising the following steps: excising 3 / 4 leaves of tissue culture seedlings ofcamellia plant tea tree, camellia oleifera or Camellia azalea and using stem segments with 1 / 4 leaf as explants, inoculating onto a somatic embryo induction medium, culturing at 24-26 DEG C under illumination to form somatic embryos at stem nodes and petioles; cutting the somatic embryos into slices of 1-5mm thick, tiling the slices onto a somatic embryo multiplication medium, and culturing at 24-26 DEG C under illumination to form new somatic embryos; transferring the somatic embryos onto a plant regeneration medium, and culturing at 24-26 DEG C under illumination to generate plantlets fromthe somatic embryos. By using the tender stem segments of camellia plants as the explants, a high-efficiency somatic embryogenesis and plant regeneration system is established, thereby effectively improving the tissue culture propagation efficiency of camellia plants. The method provides technical support for seedling production and genetic engineering improvement of crops such as tea trees, camellia oleifera, camellia, etc.

The invention discloses a method for establishing a new lycoris variety 'Taohong' tissue culture rapid-propagation technical system. According to the method, bulb blocks are taken as explants, the explants are disinfected, and the 'Taohong' tissue culture rapid-propagation technical system is established after adventitious bud induction, cluster bud induction, bud hardening, seedling hardening androoting culture, seedling hardening and transplanting steps. Compared with other original species of lycoris, when the rapid-propagation technology system is adopted to cultivate a new lycoris variety 'Taohong', the characteristic is utilized that the new lycoris variety is more resistant to high-concentration plant growth regulators, and the high-concentration plant growth regulators are used atbud induction and bud proliferation stages so that a bud germination period can be shortened, and the bud proliferation efficiency can be improved. By adopting the tissue culture rapid-propagation technology, the propagation coefficient and propagation speed of the new lycoris variety can be greatly improved, and a technical foundation is laid for application and popularization of the new lycorisvariety.

The invention discloses a novel paecilomyces lilacinus strain, as well as a preparation method and an application of same. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) at December, 28th, 2016, and is assigned the accession number CGMCC No.13379. The preparation method includes the steps of: preparing a paecilomyces lilacinus seed liquid; inoculating the seed liquid onto a solid proliferation culture medium and performing proliferation culture under a sterile aerobic condition, culture temperature being 25-45 DEG C and culture time being 5-10 days; drying the culture medium after the proliferation culture to prepare a raw bacterial agent of paecilomyces lilacinus. The novel paecilomyces lilacinus strain is used for preventing and treating nematodes on crops and has excellent capability of killing ova of the nematodes; a test proves that activity of chitinase in a fermentation product of the strain is high, so that the novel strain can be used for prevention and treatment on nematodes on roots of crops.

The invention belongs to the field of ornamental plant transgenic technology, particularly relates to a method for culturing transgenic dianthus and more particularly discloses a method for culturing transgenic dianthus by taking agrobacterium-mediated embryogenic callus of the dianthus as a material. The method comprises the following steps of embryogenic callus induction, genetic transformation, screening and regeneration, GUS (glucuronidase) coloration detection and PCR (polymerase chain reaction) detection. The method is characterized in that the GUS report gene and the ACO target gene are introduced into the receptor which is the embryogenic callus of the ianthus through agrobacterium mediation, different factors affecting the embryogenic callus transformation are screened, kanamycin or hygromycin is used as a screening agent to screen the transformed embryogenic callus, and the screening is carried out through GUS coloration, PCR detection and other auxiliary methods to obtain the transgenic dianthus. The invention provides a novel technology platform for the innovation of the germplasm resources for the genetic transformation of the dianthus.

A bryophyte tissue culture and seedling culture method based on direct spore induction comprises the steps of sporophyte selection and pretreatment, spore collection, spore direct disinfection, sporegermination induction, subculture mulitiplication of spores and protonemata, oscillatory differentiation culture, spray inoculation and seedling culture. According to the method, establishment efficiency and proliferation efficiency of a sterile system are improved, death rate and pollution rate are low, and effective seed feeding rate, subculture proliferation rate and seedling coverage rate after transplantation are high. Compared with the prior art, the tissue culture seeding death rate is reduced by 6.5%, the pollution rate is reduced by 70.4%, and the effective seeding rate is increased by 45.4%. The subculture proliferation rate is increased by 2.63 times, and the coverage rate of bryophyte plants cultured from seedlings reaches 85.6% on average.

The invention discloses a method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate a plant. By changing medium components, light condition and subculture interval of suspension culture in the prior art, an embryogenic cell cluster can proliferate 2.82-3.02 times per week and proliferate 83 times per month, the proliferation efficiency is improved by more than 15 times compared with that in the prior art, the plant regeneration operating process is simplified, and the plant regeneration of the embryogenic cell cluster can be completed by only one step; meanwhile, the plant regeneration time is shortened, and is 6-10 weeks, which is over 20 days shorter than that in the prior art.

The invention discloses a method for inducing human umbilical cord mesenchymal stem cells into testicular interstitial cells and application thereof. The method comprises the following steps: cloning mouse steroidogenic factor-1 (SF-1) gene into an adenovirus shuttle plasmid pAdtrack-CMV-EGFP to construct a recombinant vector pAdtrack-CMV-SF-1; recombining the recombinant vector pAdtrack-CMV-SF-1 and an adenovirus carrier plasmid pAdEasy-1 to obtain an adenovirus plasmid pAd-SF-1 carrying SF-1 gene; digesting and linearizing the adenovirus plasmid pAd-SF-1, and packaging with human embryonic kidney cells AD-293 to obtain adenovirus; adding the adenovirus into an induction culture medium to infect human umbilical cord mesenchymal stem cells, and carrying induced differentiation of the human umbilical cord mesenchymal stem cells to testicular interstitial cells. Through the induction using the method of the invention, human umbilical cord mesenchymal stem cells can in vitro differentiate into testicular interstitial cells only in one week, so as to provide an important cell source for treatment of testosterone deficiency using cell replacement or genetic method.

The invention provides a canine parvovirus proliferation method. According to the method, F81 cells are creatively taken as host cells of canine parvovirus, so that virus proliferation is facilitated especially; a riptide-type bioreactor is adopted, so that compared with conventional spinner bottle modes, processes are easier to control, and products are more stable in quality; a microcarrier culture mode is adopted, so that a culture solution can be utilized fully, growing characteristics of adherent cells are maintained, and high-density culture effect is realized. In addition, aiming at own characteristics of the F81 cells and the canine parvovirus and culture environment of the bioreactor, specific culture conditions are matched, and components of the culture solution, inoculation amount and condition parameters are designed in an optimized manner, so that canine parvovirus proliferation efficiency is improved remarkably. Protruding technological effect is realized through an ingenious technological concept, and the method is low in cost and easy in realization and has outstanding popularization prospect.

The invention provides a microorganism expanding culture device for water environment treatment. The microorganism expanding culture device comprises an equipment shell, an automatic quantitative microbial agent adding system, a raw water quality filtering and disinfecting system, a clear water supply system, a microbial agent constant-temperature culture system and an automatic control system, the microbial agent automatic quantitative adding system, the raw water quality filtering and disinfecting system and the clear water supply system are all connected with the microbial agent constant-temperature culture system through pipelines; the automatic control system is provided with an operation control program and operation requirements in advance. According to the invention, the effective flora to be supplemented and added into the water body is subjected to enlarged culture by controlling key environmental factors such as temperature, dissolved oxygen and the like which influence microbial proliferation, so that under the condition of the same dosage of the microbial agent, the number and the biological activity of the effective flora added into the water body are increased, and the treatment effect is improved.

The invention provides a method for quickly propagating seedlings of Dendrobium spp. by tissue culture. The method uses new buds of Dendrobium spp. as explants to arrive at quick propagation by means of induction of cluster buds and growth of buds from buds. The method comprises the steps of selecting explants, sterilizing the explants, subjecting cluster buds to induction culture, subjecting the cluster buds to enrichment culture, performing rooting culture, and transplanting test tube seedlings. The seedlings of Dendrobium spp. can be acquired by using the method just in 5-6 months; induction rate in the propagation process is high, subsequent rooting culture after the propagation can be performed without strong seedling culture, the culture links are simplified, culture cost is reduced, multiplication efficiency is high, and the efficiency of the whole seedling culture is improved; in addition, the test tube seedlings cultured herein grow robust with developed roots, and may gain high environmental adaptability through acclimatization, so that seedling transplanting survival rate is increased.

The invention discloses a culture medium for culturing urine-derived cells, and belongs to the field of cell culture. The culture medium contains human blood platelet lysates. The culture medium has the advantages that the fact that the human blood platelet lysates can be used as core additives for the heterology-free urine-derived cell culture medium is ultimately confirmed via extensive screening experiments by applicants over the years, and accordingly the problems of extremely low urine-derived cell proliferation speeds, gradual cell death and the like during heterology-free culture in thepast can be solved by the aid of the culture medium; the human blood platelet lysates are added in the heterology-free culture medium, the urine-derived cells can be effectively cultured by the aid of the heterology-free culture medium, and the requirements of research or clinical application on the cell quantities can be met; the blood platelet lysates are innovatively applied to urine-derived cell heterology-free culture and the culture medium, and the culture medium has important significance in regenerative medicine.

The method of differentiating human bone marrow matrix stem cell to form insulin cell includes culturing and screening fresh human bone marrow to obtain human bone marrow matrix stem cell; amplifying human bone marrow matrix stem cell; inducing the amplified human bone marrow matrix stem cell with inducing agent beta-mercaptoethanol and / or niacinamide to obtain insulin cell formed by differentiated human bone marrow matrix stem cell. The bone marrow matrix stem cell may taken from the patient himself, and this can avoid graft rejection and corresponding cell function failure. The present invention has high repeatability and rich material source.

The invention discloses a culture medium for efficiently culturing umbilical cord blood CIK cells in vitro and a culture method thereof, which belong to the technical field of biology. The culture medium is prepared from the following components: an AlyS505N-0 serum-free basal culture medium, a cytokine composition A and a natural traditional Chinese medicine extract. The culture method comprisesthe following steps: collecting and separating umbilical cord blood mononuclear cells; resuspending cells, adding IFN-gamma for activation, incubating in a 6% CO2 incubator at 37 DEG C, adding the cytokine composition A for stimulation on the next day, supplementing the culture medium containing a traditional Chinese medicine extract B after 3-5 days, supplementing the culture medium containing the traditional Chinese medicine extract composition B every 2-3 days during culture, and co-culturing for 15-17 days to obtain a large number of CIK cells. According to the culture medium and the culture method thereof, a large number of CIK cells can be proliferated, and CD3+CD56+ double positive cells in the cells can reach 70% or above.