The invention discloses a high-efficiency method for the induction and the successive transfer proliferation regeneration of banana embryogenic calluses. The technological process comprises a culture process of inducing dedifferentiated calluses, a culture process of inducing fragile embryogenic calluses, a successive transfer proliferation process of the fragile embryogenic calluses, an inducing and embryo-forming process of the embryogenic calluses and an inducing and seedling process of mature embryos. The invention has the advantages of simple technological process, low production cost, high embryogenic tissue induction and proliferation rate and long successive transfer time, solves the problems of low embryonic reaction of an exophyte, hard induction of the embryogenic calluses, low induction embryo-forming and seedling rates, long induction period, and the like in the culture process of banana tissues, provides a new technological route for a banana transform technology and provides a high-efficiency regeneration system for producing juvenal tissue and culturing banana seedlings, thereby having a great application value.