61 results about "Bone marrow stromal stem cell" patented technology

The present invention is directed to a new bone marrow stromal stem cell (BMSSC) marker, CD18, for use in selecting a population of cells enriched in BMSSCs, from bone marrow cells, adipose cells, or peripheral blood. The invention is further directed to methods for selecting a population of cells enriched in BMSSCs based on the selective expression of CD18 on their surface, using techniques known in the art such as fluorescent assisted cell sorting, an immunomagnetic method, flow microfluorimetry, immunofluorescence, immunoperoxidase staining, radioimmunoassay and immunoaffinity chromatography. The invention is further directed to the BMSSCs isolated based on CD18 expression, and their use to treat various diseases. In one aspect, the HMSSCs are transformed with a vector having a normal gene for CD18, and the transformed BMSSCs are administered to treat bone degenerative diseases and diseases of bone involving abnormal expression of CD18 expression of CD18.

The invention relates to a medical permanent implantation object, in particular to a medical implantation porous tantalum rod used for treating early-mid femoral head necrosis. The porous tantalum rod comprises a body, wherein one end of the body is provided with a groove, the other end of the body is provided with a threaded structure, and a notch is formed in the end surface of the tail end of the threaded structure. One end of the body of the porous tantalum rod is provided with the notch, bone marrow stromal cells or bone marrow stromal cell united biological membrane complexes and the like can be loaded, the generation of a new bone is favorably realized, a better clinical effect is particularly realized in the early femoral head necrosis treatment, and the defects of poor clinical treatment effect and the like of the existing entity type porous tantalum rod are overcome. The porous tantalum rod has the advantages that the preparation method is simple, the cost is low, the implementation and the clinical application are favorably realized, and the porous tantalum rod is suitable for the need of wide patients.

A surface treatment method of a tissue-engineered bioactive implant comprises the following steps of: (1) obtaining marrow stromal cells from autologous ilium or obtaining periodontal ligament stem cells from a fresh healthy tooth which is pulled off; (2) putting the marrow stromal cells or the periodontal ligament stem cells into a culture dish in which Alpha-MEM culture medium is added, and adding factors promoting the secretion of extracellular matrix into the marrow stromal cells or the periodontal ligament stem cells so as to form marrow stromal cell aggregate or periodontal ligament stem cell aggregate; (3) wrapping an implant with the marrow stromal cell aggregate or the periodontal ligament stem cell aggregate; (4) placing the wrapped implant in the culture medium; and (5) adding vitamin C and hexadecadrol into the culture medium to promote the secretion of the extracellular matrix, thereby causing the marrow stromal cell aggregate or the periodontal ligament stem cell aggregate together with the implant to form tissue-engineered implant with bioactivity. The surface treatment method solves the problem that patients suffering from osteoporosis and diabetes and recovering from radiation treatment have low implant success rate.

The present invention discloses one kind of injected tissue engineering bone and its construction and application. The injected tissue engineering bone consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg / ml concentration fibrinogen solution in the ratio of 1 to 3-6, and contains marrow matrix stem cell in the concentration of 5*10<>6< / >-10*10<>7< / > / ml; and the solution II is solution mixture of calcium chloride in 40 mmol / ml concentration and thrombin in 400-1000 U / ml concentration. When the injected tissue engineering bone is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected tissue engineering bone is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

The present invention relates to a method capable of utilizing internal perfusion to induce bone marrow matrix stem cell to construct tissue engineering bone. Said method includes the following steps: (1), extraction, separation and culture of bone marrow matrix stem cell; (2), preparation of coral composite artificial bone; and (3), creation of perfusion system.

The invention discloses a bioactivity bracket and a preparation method thereof. The bioactivity bracket is obtained through the following steps of firstly, loading bone shape generation proteins on ahollow mesoporous hydroxylapatite microsphere through a grafting manner or an adsorbing manner, so as to obtain a drug bearing microsphere, then mixing the drug bearing microsphere into a bracket matrix solution, then performing printing through a 3D printing technique to prepare a compound bracket, and finally, adsorbing chemotactic growth factors on the surface to obtain the bioactivity bracket;or firstly, blending the drug bearing microsphere and the chemotactic growth factors in the bracket matrix solution, and then performing printing through the 3D printing technique to prepare the bioactivity bracket. Through the adoption of the method disclosed by the invention, the obtained bioactivity bracket can realize time sequence releasing of various growth factors: the chemotactic growth factors are released in an early stage, and bone repair cells are recruited to a damaging region; and bone shape generation proteins are mainly released in the later stage, and bone marrow substrate stem cells which are recruited to the damaging region are induced to be subjected to osteogenic differentiation, so that regeneration and repairing potential of an autologous tissue is sufficiently mobilized, and regeneration and repairing of bone defect are promoted.

The present invention belongs to the field of artificial organ constructing technology in medicine engineering, and is especially one kind of tissue engineering bone and its construction and application. The tissue engineering bone of the present invention includes carrier rack and seed cell attached to the carrier rack to form complex with the 3D structure and bioactivity of bone tissue. The carrier rack is modified PLGA through deacidification treatment to have great pores and high porosity, and has cell factor bone morphogenesis protein (PMP) carried; and the seed cell is marrow matrix stem cell. The tissue engineering bone of the present invention may be used in constructing functional tissue engineering bone graft for repairing large bone defect.

The invention provides a sulfonic quantum dot / silk fibroin composite nanofiber membrane and a preparation method and application thereof. The preparation method comprises the following steps that degumming treatment is performed on natural silk, and silk fibroin is obtained; then, fibroin is dissolved in a lithium bromide water solution, and a silk fibroin sponge is obtained through dialysis, centrifugal and freeze drying; the silk fibroin sponge is dissolved into formic acid to be mixed to be uniform, then, a sulfonate graphene quantum dot water solution is ultrasonically and uniformly dispersed into a silk fibroin-formic acid solution, and a composite nanofiber membrane is prepared through electrostatic spinning equipment; mouse fibroblasts are used for performing cell viability evaluation on the composite nanometer member, and it shows that the composite nanometer membrane has the excellent biocompatibility. The prepared composite fiber membrane has the good biocompatibility and degradability, has the potential for promoting proliferation and differentiation of human bone marrow storm stem cells, and has the good biologic medical material application prospect.

The invention discloses a bioactive ceramic material with degradability. The chemical formula of the bioactive ceramic material is Ca7Si2P2O16. A preparation method for the bioactive ceramic material comprises the following procedures: A) synthesizing pure Ca7Si2P2O16 powder by using a sol-gel method; and B) adding polyvinyl alcohol into the Ca7Si2P2O16 powder for granulation, dry pressing to obtain a blank, and calcining the blank at high temperature to obtain the Ca7Si2P2O16 bioactive ceramic material. The nagelschmidtite ceramic can support adherence and proliferation of bone marrow stromal cells. Compared with the traditional beta-tricalcium phosphate ceramics, the bioactive ceramic material can better promote osteogenic differentiation of the bone marrow stromal cells and has higher degradability.

The invention relates to a filtering method, in particular to a method for quickly filtering and screening bone marrow stromal cells. The method adopts the technical proposal that the method for quickly filtering and screening the bone marrow stromal cells comprises the following steps: firstly, performing anticoagulant treatment on bone marrow blood to be used; secondly, filtering the bone marrow blood after anticoagulant treatment through a filtration recombiner; and thirdly, collecting stem cell-frame composite materials. The method has the advantages that the method quickly recombines the cells and a vector (namely a filter screen material) when filtering and screening the bone marrow stromal cells simultaneously, so as to integrate the two procedures of cell screening and cell-vector combination into a whole. The filtering method has short time consumption, does not need in vitro culture, can be simultaneously performed in an operation, and is more favorable for clinic use because the volume of the screened stromal cells is smaller than that of cell suspension finally obtained by density gradient centrifugation technology.

The invention relates to an injection organism repair material and relative production. Wherein, it comprises carrier support and seed cell adhered on the carrier support to form skeleton three-dimension structure and biological active composite; and it uses the injection chitose-beta-tricalcium phosphate composite as carrier support to couple seed cell; the seed cell is bone marrow substrate stem cell. The test has proved that the chitose-beta-tricalcium phosphate composite has better compatibility with BMSCs.

The present invention is directed to a new bone marrow stromal stem cell (BMSSC) marker, CD18, for use in selecting a population of cells enriched in BMSSCs, from bone marrow cells, adipose cells, or peripheral blood. The invention is further directed to methods for selecting a population of cells enriched in BMSSCs based on the selective expression of CD18 on their surface, using techniques known in the art such as fluorescent assisted cell sorting, an immunomagnetic method, flow microfluorimetry, immunofluorescence, immunoperoxidase staining, radioimmunoassay and immunoaffinity chromatography. The invention is further directed to the BMSSCs isolated based on CD18 expression, and their use to treat various diseases. In one aspect, the HMSSCs are transformed with a vector having a normal gene for CD18, and the transformed BMSSCs are administered to treat bone degenerative diseases and diseases of bone involving abnormal expression of CD18 expression of CD18.

A dog stem cell excreted factor repair liquid capable of quickly healing dog wound tissue relates to the technical field of biology and especially to the technical field of dog stem cells. The repair liquid comprises a dog stem cell active component extract which is extracted from dog bone marrow substrate stem cells and includes various active growth factors, collagen, active polypeptide, micro elements, hyaluronic acid and the like. The repair liquid can quickly promote growth, activation and regeneration of cells and tissue and can achieve a net-typed adjustment effect on treatment of dog refractory wounds. The dog stem cell excreted factor repair liquid can quickly and effectively promote a wound on skin and a surgical wound to heal, can repair ulceration on skin and eyes and has a good scientific and clinical value.

The present invention provides a tissue engineering tendon graft. It includes: (a). pharmaceutically-acceptable bio-degradable material.; (b) seed cell, said seed cell can be inoculated in the described bio-degradable material and is selected from (i) fibroblast; and (ii), mixture of fibroblast and tendon cell and / or bone marrow matrix stem cell according to the ratio of 1:10-10000:1; and (c) bio-membrane, the exterior of said bio-degradable material is covered with said bio-membrane. Said invented graft can beused for effectively curing musculotendinous defect. Said invention also provides its preparation and application.

The invention discloses an anti-infection calcium phosphate composite bone cement material and a preparation method thereof. The preparation method comprises the following steps: enabling gamma-polyglutamic acid modified by phosphorylcholine to adsorb nano-silver, and carrying out graft copolymerization reaction between water-soluble polysaccharide and gamma-polyglutamic acid to prepare a nano-silver anti-infection agent with an anti-infection effect; and on the basis that the basic characteristics of calcium phosphate bone cement are not affected, compounding and blending the anti-infection agent and other assistants with medical calcium phosphate bone cement, and then preparing the calcium phosphate composite bone cement material with an anti-infection function by adopting a molding method. The method disclosed by the invention is simple in process, and the compressive strength of the prepared calcium phosphate composite bone cement material reaches 70MPa; the calcium phosphate composite bone cement material does not have obvious cytotoxicity and blood coagulation to osteoblasts and bone marrow stromal cells and does not have obvious sensitization, stimulation and genetic toxicity; and the calcium phosphate composite bone cement material has a wide antibacterial spectrum and has certain antibacterial effects on Gram-positive cocci and Gram-negative bacilli, and the antibacterial rate reaches 98%.

The present invention discloses a tissue engineering soft bone graft. It includes: (a). pharmaceutically-acceptable bio-degradable material; and (b) mixture of soft bone cell and bone marrow stemcells, BMSCs, and the mixing ratio of the described soft bone cell and bone marrow stemcells is 1:9-5:5. Said invention can effectively cure defect of soft bone. Said invention also provides its preparation method and application.

The application discloses an injectable tissue-engineered bone repair material and a construction method thereof, wherein the injectable tissue-engineered bone repair material comprises a gel carrierand seed cells; the seed cells are attached to the gel carrier to form a complex with bone tissue three-dimensional structure and physiological activity; the gel carrier is made from injectable sodiumalginate and fibrinogen mixture and used for combining with the seed cells; the seed cells are bone marrow stroma stem cell microspheres. Single cells are replaced with the cell microspheres to act as the seed cells, so that the problem that single cells have low inoculation density and poor anoxia resistance is solved; therefore, osteogenic capability of the injectable tissue-engineered bone repair material is improved.

The invention relates to a filtration recombiner, in particular to a parallel circulation cell selective filtration recombiner. The parallel circulation cell selective filtration recombiner adopts the technical proposal that the parallel circulation cell selective filtration recombiner comprises an injection pump, a first pipe, a second pipe, a first right connecting pipe, a first left connecting pipe, a second right connecting pipe, a second left connecting pipe, a first bidirectional crack valve, a second bidirectional crack valve, a switch valve, an exhaust hole and a peristaltic pump, and also comprises a filtration box which is provided with a filter screen and a movable cover. The parallel circulation cell selective filtration recombiner has the advantages of needlessness of antibody and antigen reaction, needlessness of using beads and other media, needlessness of in vitro culture, needlessness of special centrifugal equipment, quick speed, and utilization of the adhesivity of bone marrow stromal cells to perform bone marrow stromal cell filter-type quick screening on marrow blood.

The invention provides a biological packed agent to treat early avascular necrosis of femur head, which is mainly composed of fibrinogen, prothrombin and the bone marrow stromal stem cells of the transferred HGF gene. The reagents are injected into the rabbit femoral necrosis through the decompression tunnel with the duplex injector by the core decompression technology. The fibrinogen forms a three-dimensional network structure gel and then becomes the stent for transfer HFG gene bone marrow stromal cells and controls the cell secretion HFC slow release to prevent the loss of the transplanted transgenic bone marrow stromal cell under the effect of the prothrombin. In this way, the agent can keep HGF partial effect density and improve the blood vessel regeneration and bone reconstruction in the necrosis area.

The present invention discloses a tissue engineering soft bone graft. It includes: (a). pharmaceutically-acceptable bio-degradable material; and (b) mixture of soft bone cell and bone marrow stemcells, BMSCs, and the mixing ratio of the described soft bone cell and bone marrow stemcells is 1:9-5:5. Said invention can effectively cure defect of soft bone. Said invention also provides its preparation method and application.

A tissue-engineered bone containing innervation used for repairing dysostosis with high speed and effect has a carrier scaffold and the seed cells attached to said carrier scaffold to form a bioactive 3D bone tissue structure. Said carrier scaffold is a biodegradable material containing innervation. Said innervation is to implant the sensory nerve tracts into said carrier scaffold. Said seed cell is the stem cell of bone marrow matrix. Its configuring process is also disclosed.

The invention discloses an injectable bone repair material and a preparation method therefor, and belongs to the technical field of constructing artificial internal organs through a tissue engineering method in biomedical engineering. The injectable bone repair material is a composite body composed of a support material and seed cells, with a stereo structure and physiological activity. Injectable fibrin glue is employed as the support materials, and human autologous marrow matrix stem cells are cultured to obtain seed cells. The seed cells are human marrow matrix stem cells. The histocompatibility of the human marrow matrix stem cells and fibrin glue is good, and the composite constructed bone repair material can cure clinic bone ununion successfully. The repair material integrates advantages of a cell therapy and the support material, a new means is employed, a bone ununion therapeutic method with minimal injury and complications is provided, and the repair material has wide clinic application prospects.

The present invention relates to medical tissue engineering, and is process of preparing tissue engineering cartilage with inner support for use as permanent prosthesis clinically. The process includes the following steps: making inner support with nondegradable stable medical porous high-density polyethylene MEDPOR, coating the inner support with nonwoven net of degradable polyglycolic acid (PGA) with high biocompatibility to form compound MEDPOR-PGA rack, dropping prepared autologous marrow matrix stem cell suspension to the nonwoven PGA net, in vitro culturing to the inducing solution with DMEM cartilage connecting TGF-beta1,IGF-I and insulin for certain time, and implanting to under skin for 4-6 weeks to constitute the tissue engineering cartilage. The present invention makes it possible to prepare clinically essential permanent implant.

The invention relates to a pure titanium dental implant, which is characterized in that a plurality of micron-sized grooves are formed in the surface of the pure titanium dental implant, and nanotubesperpendicular to the central axis of the dental implant are arranged in the grooves. A width of each groove is 50 [mu] m, a depth of each groove is 10 [mu] m, and the distance between adjacent grooves is 50-53 [mu] m. The method comprises the following steps: firstly, a middle groove in the surface of the dental implant is manufactured; then manufacturing the nanotube is manufactured on the groove so that the surface of the top of the nanotube is an inclined plane, and a contact surface between the inclined plane and a bone tissue interface and between the inclined plane and a soft tissue interface is large; the contact surface between the nanotube and a bone tissue interface and between the nanotube and a soft tissue interface is large, the micron-sized groove can induce bone tissue cells and soft tissue cells to better grow along the grooves, and the nanotube is also beneficial to adsorption of protein, oxygen and nutrients. The size of the groove is just slightly smaller than thatof the bone marrow stromal stem cells and gingival fibroblasts which are not stretched, so that the cells cannot fall into the groove, and the cells can grow along the groove. The proliferation of gingival fibroblasts and the expression of soft tissue closure related genes can be promoted, so that the effects of osteogenesis and soft tissue closure are achieved.

Disclosed are an artificial bone and a manufacturing method of the artificial bone. Cell membranes form a prototype of the artificial bone, the cell membranes are cultivated in culture fluid of inducing action, and then the cell membranes are mutually engaged, finally the artificial bone is achieved. The composition of the artificial bone comprises bone marrow stromal cells after being induced and stromas which are secreted by the cells, histology represents a chondroid tissue, the coloration of safranine -O shows a positive reaction, and when the artificial bone is implanted into a hypoderm of a naked mouse, a mature bone tissue can be formed. The invention solves technical problems that alternate materials of bones of the background technique produce chronic inflammation reaction or rejection owning to the existing of a support, the long term remaining of foreign matters, or the degrading of products. According to the sizes and shapes of damaged bones, a reproduced bone tissue without supports are a complete biological tissue of the functions of safe and reliable usage and being capable of completely replacing damaged bones.

The present invention is immortal human bone marrow matrix stem cell line and its establishing process. The primary human bone marrow matrix stem cell from normally aborted fetus bone marrow tissue is single cell cloned and cultured in a finite dilution process to purify primary cell and transfected with pCIneo-hTERT gene for immortalizing. The human bone marrow matrix stem cell line is extracorporeal cultured, may be infinitely cloned and proliferated and is normal cytologic cell without conversion cell or cancer cell characteristic. The cell may be directionally induced and differentiated into bone cell, cartilage cell or cardiac muscle-like cell and has multiple direction differentiating latent and adult stem cell characteristic. It may be applied in biological engineering field. The present invention provides standard cell line for the biological research of biological behavior of human bone marrow matrix stem cell, platform for basic tissue engineering research, enough seed cells for clinical application and effective method for relevant research.

The invention discloses an application of B-ecdysterone in making medicine for curing and / or preventing osteoporosis. The invention respectively studies the protective action of B-ecdysterone on bone metabolism at both general level and cellular level. When cultured together with osteoblast strain MC3T3-E1 and primary mice marrow stromal stem cell, B-ecdysterone can obviously promote the osteogenic differentiation of both cells. A mice osteoporosis model is established through tretinoin lavage, and the in vivo activity experiment of B-ecdysterone is carried out through adopting an intraperitoneal injection method and a lavage method; the results show that B-ecdysterone has the functions of increasing bone density, the content of mineral matters in bone tissues and thighbone diameter and improving hydroxyproline enzymatic activity. Therefore, B-ecdysterone can be used to make medicine for curing and / or preventing osteoporosis as well as medicine for promoting osteogenic differentiation and bone healing.

The invention discloses a construction method of a tissue engineering bone repair material. The method includes: 1) culture of seed cells; 2) preparation of an injectable scaffold material; 3) treatment of the seed cells; and 4) compounding of the seed cells and chitosan-beta-tricalcium phosphate, and other steps. The bone repair material provided in the invention is composed by compounding an injectable material with bone marrow stroma stem cells, can be arbitrarily shaped and cell compounding is simple. Being able to be completely degraded and absorbed within certain period of time, the material has an obvious inductive osteogenesis effect, and the cost is low.

The invention relates to non-virus gene transfer vector. Its structure is as follows: NH2-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-Gly-Arg-Gly-Asp-Ser-Pro-Cys-COOH. The invention has good target direction property for non virus gene transfer vector, high transfection efficiency, and can linkage with bone matrix material to increase cell specificity adherence and form gene carrying material to realize cell directional transfection. The invention also discloses the non-virus gene transfer vector manufacturing method and its application in medicine experimental study and clinic gene therapy.