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Novel use of ecdysterone

A technology of ecdysterone and medicine, applied in the field of ecdysterone, can solve the problems that there are no basic or clinical research reports on the impact of β-ecdysterone on bone metabolism

Active Publication Date: 2008-11-12
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0009] Western scholars' research on phytoestrogens is mainly limited to soybean isoflavones. At present, there are no basic or clinical research reports on the effects of β-ecdysterone on bone metabolism, and there are no reports on the application of β-ecdysterone based on its estrogen-like effects.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0032] In vivo activity test of embodiment 1, β-ecdysterone

[0033] 1. Construction of mouse osteoporosis model

[0034] Select 50 Kunming mice, male, 2 months old, SPF grade, weighing about 40 ± 5g (Experimental Animal Center of Guangdong Medical College) (the center has been identified by the Guangdong Provincial Laboratory Animal Monitoring Institute, and various SPF grade mice can be sold to the outside world. Mice) were randomly divided into 5 groups: normal control group, retinoic acid model group, low-dose β-ecdysterone intragastric administration group, high-dose β-ecdysterone intragastric administration group, and intraperitoneal injection β-ecdysterone group, with 10 mice in each group. Except for the normal control group, the rest of the groups were intragastrically administered retinoic acid (70 mg / kg body weight) once a day in the morning to establish models. One week after modeling, the three groups were given β-ecdysterone by intragastric administration of low d...

Embodiment 3

[0044] Example 3, using osteoblast cell line MC3T3-E1 and mouse primary bone marrow stromal stem cells (MSC) as models, detecting the activity of β-ecdysterone in promoting bone differentiation

[0045] 1. β-ecdysterone enhances the activity of alkaline phosphatase (ALP)

[0046] The β-ecdysterone obtained in Example 1 was dissolved in ethanol, and diluted to 10 with DMEM medium when in use. -5 -10 -8 M (ie 4.67*10 -3 g / L-4.67*10 -6 g / L), added to cultured osteoblast cell line MC3T3-E1 (purchased from Cell Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences) or mouse primary MSC (Xiang-huiLI, JIN-chao ZHANG, Sen-fang SUI , et al. Effect of daidzin, genistin, and glycitin onosteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts. Acta Pharmacologca Sinica, 2005, 26(9): 1081-1086) culture plate. The medium was changed every three days, and the activity of ALP in the culture medium ...

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Abstract

The invention discloses an application of B-ecdysterone in making medicine for curing and / or preventing osteoporosis. The invention respectively studies the protective action of B-ecdysterone on bone metabolism at both general level and cellular level. When cultured together with osteoblast strain MC3T3-E1 and primary mice marrow stromal stem cell, B-ecdysterone can obviously promote the osteogenic differentiation of both cells. A mice osteoporosis model is established through tretinoin lavage, and the in vivo activity experiment of B-ecdysterone is carried out through adopting an intraperitoneal injection method and a lavage method; the results show that B-ecdysterone has the functions of increasing bone density, the content of mineral matters in bone tissues and thighbone diameter and improving hydroxyproline enzymatic activity. Therefore, B-ecdysterone can be used to make medicine for curing and / or preventing osteoporosis as well as medicine for promoting osteogenic differentiation and bone healing.

Description

technical field [0001] The invention relates to a new application of ecdysterone (β-ecdysterone). Background technique [0002] Osteoporosis (OP for short) is a systemic and systemic bone disease characterized by decreased bone mass and microstructural destruction of bone tissue leading to bone fragility and increased risk of fracture. Symptoms of systemic diseases of bone metabolism disorders. Osteoporosis is a common and frequently-occurring disease among middle-aged and elderly people. As the global aging is getting worse, the international medical community has listed osteoporosis, diabetes and cardiovascular disease as common diseases that seriously endanger the health of the elderly. Primary osteoporosis is a systemic skeletal disease characterized by decreased bone mass and degeneration of bone microstructure, resulting in increased bone fragility and fracture risk. According to a survey by the World Health Organization, in EU countries, one person suffers from ost...

Claims

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Application Information

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IPC IPC(8): C07J9/00A61K31/575A61P19/10
Inventor 史小军高磊聂勇
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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