189 results about "Tissue engineered bone" patented technology

The invention relates to the use of Fused Deposition Modeling to construct three-dimensional (3D) bioresorbable scaffolds from bioresorbable polymers such as polycaprolactone (PCL), or from composites of bioresorbable polymers and ceramics, such as polycaprolactone / hydroxyapatite (PCL / HA). Incorporation of a bioresorbable ceramic to produce a hybrid / composite material support provides the desired degradation and resorption kinetics. Such a composite material improves the biocompatibility and hard tissue integration and allows for increased initial flash spread of serum proteins. The basic resorption products of the composite also avoids the formation of an unfavorable environment for hard tissue cells due to a decreased pH. The scaffolds have applications in tissue engineering, e.g., in tissue engineering bone and cartilage.

The present invention discloses process of preparing integral bionic tissue engineering bone-cartilage rack with functional interface. The process includes the first establishment of CAD model of the integral bone-cartilage rack by means of bionic principle; preparing collagen / chitosan and collagen / hydroxyapatite micro polymer powder; converting the CAD model into fast forming file input into a 3D printing fast forming machine, spreading the powder with one powder spreader, spraying pentanedial adhesive selectively on the spread powder for adhering and eliminating excessive powder to obtain the integral bionic tissue engineering bone-cartilage rack with functional interface. The present invention has excellent biocompatibility, controllable cartilage rack degrading speed and mechanical strength and other advantages.

The invention discloses a 3D-printed gradient-diameter medical porous metal bone tissue scaffold and aims to solve the problems that a single repeated microporous structure in the prior art is adverse to bone tissue ingrowth and a bone tissue implant has difficulty in bony healing with self-bones. The scaffold is in a hexahedron structure as a whole, and comprises components A, components B and components C in tight arrangement, wherein the components A are arrayed on an outermost layer of the hexahedron structure; the components B are arrayed on a secondary outer layer; and the components C are arrayed on the innermost layer; all the components A, B and C are in a hexahedron frame structure; the pore diameter of the components A is greater than that of the components B; and the pore diameter of the components B is greater than that of the components C. Through the reinforcing structural design, the gradient-pore tissue engineering bone scaffold is produced; through regulating the ingrowth of bone tissues, fibroblasts and the like by gradient-change pores, the optimal bone healing can be achieved finally.

The invention relates to the use of Fused Deposition Modeling to construct three-dimensional (3D) bioresorbable scaffolds from bioresorbable polymers such as polycaprolactone (PCL), or from composites of bioresorbable polymers and ceramics, such as polycaprolactone / hydroxyapatite (PCL / HA). Incorporation of a bioresorbable ceramic to produce a hybrid / composite material support provides the desired degradation and resorption kinetics. Such a composite material improves the biocompatibility and hard tissue integration and allows for increased initial flash spread of serum proteins. The basic resorption products of the composite also avoids the formation of an unfavorable environment for hard tissue cells due to a decreased pH. The scaffolds have applications in tissue engineering, e.g., in tissue engineering bone and cartilage.

The invention discloses a tissue engineering bone / cartilage double-layer scaffold and a construction method and application in medical science. The method adopts natural cartilage and bone as materials, respectively removes the antigenicity of the cartilage and the bone through de-cell, and adopts a freezing and drying method to prepare the double-layer scaffold simultaneously suitable for growthof the two issues of the bone and the cartilage. A preparation method comprises that: acellular bone is soaked in suspension of cartilage acellular matrix microfilaments, and the double-layer scaffoldof which the upper layer is a cartilage acellular matrix three-dimensional porous spongy scaffold and the lower layer is an acellular bone scaffold is prepared by the freezing and drying method and acrosslinking method. The cartilage acellular matrix spongy scaffold part of the double-layer scaffold can be introduced with factors promoting the formation of the cartilage, and the acellular bone part can be introduced with factors promoting the formation of the bone, and the factors can be respectively inoculated with cells for forming the cartilage or the bone to construct a living tissue engineering bone / cartilage complex, which is used for repairing bone and cartilage complex defect or total joint defect clinically.

The invention discloses a tissue engineering bone cartilage composite bracket and an integrated photocuringable forming method thereof. The tissue engineering bone cartilage composite bracket serves as a ceramic bracket, wherein the ceramic bracket is served as a bone-repair part, and a porous structure is arranged on the surface of the ceramic bracketbone-repair part of the porous structure; and the ceramic bracket is fixed on a supporting plate of a photocuringable quick forming machine. A laser scanning path is driven through a computer aided design (CAD) model; and laser directly exposes and cures a hydrogel bracket and a pure hydrogel bracket which contain a ceramic component onto the ceramic bracket sequentially to form a three-layer composite bracket. Therefore, quick and fine preparation of a three-dimensional composite bracket of bone cartilage-like tissue histomorphometry is realized; uncontrollable structures and low efficiency because of handwork in the preparation process of a traditional composite bracket are avoided; the preparation efficiency is improved; and a calcified cartilage-like layer bracket and a hydrogel cartilage bracket with subtle structures can be bonded and compounded on the surface of the ceramic bracket by means of a rivet and a two-phase material.

The invention provides fibers for tissue engineering bone repairing, a tissue engineering bone repairing scaffold and a preparation method thereof. The fibers for the tissue engineering bone repairing, provided by the invention, have a double-layered structure comprising an inner layer and an outer layer; the inner layer provides suitable mechanical strength for the whole fibers, and the outer layer can effectively maintain the activity and functionality of cells; the fibers for the tissue engineering bone repairing are applied to preparation of the tissue engineering bone repairing scaffold;the prepared tissue engineering bone repairing scaffold can be used for keeping the activity of the cells very well, has excellent rapid bone formation and blood vessel formation functions and has good mechanical properties.

Compositions, methods of production and use, and kits for an osteochondral graft involving both articular cartilage and underlying bone are provided.

The invention discloses a tissue-engineered bone based on an entochondrostosis system and a construction method thereof. The construction method is characterized in that mesenchymal stem cells are cultivated on a porous bone scaffold material to construct a tissue-engineering complex, subjected to in vitro cartilage differentiation induction cultivation for two weeks and continuously conducted with fat cartilage differentiation induction cultivation for two weeks to obtain the tissue-engineered bone. According to the invention, an in vivo research result shows that the in vivo ectopic bone formation can be realized, bone defects can be successfully repaired, the difficulties that the uniform vascularization of large piece of tissue-engineered bone cannot be realized and enough nutrition can't be supplied are solved, and the construction method has a very good application prospect in the construction of the tissue-engineered bone and bone defect repairing.

The invention discloses a method for preparing a tissue-engineered bone / cartilage integrated scaffold. An integrated scaffold structure of which internal pores are aligned and pore diameters are changed in a gradient way along a thickness direction and which has good mechanical properties is obtained by simulating the composition and spatial structure characteristics of natural articular cartilage and using extracellular matrixes and bone matrix gelatin of the cartilage as main raw materials by methods such as a different concentration superposition method, a directional crystallization method, a freeze-drying method, a cross-linking reaction method and the like. The high biocompatibility of the scaffold is favorable for the adhesion and proliferation of cells; a special pore structure of the scaffold ensures that the content of matrixes secreted by the cells is also changed in a gradient way; and meanwhile, a bone matrix gelatin substrate provides good mechanical support for the instant fixation of the scaffold and an injured part.

The invention relates to a culture medium for culturing cells, in particular human cells in a process for tissue engineering bone. The medium comprises glucose, a mineral, a vitamin, a growth factor and L-glutamine, wherein the L-glutamine is present in a concentration of at least 300 mg / L.

The present invention relates to histoengineering bone comprising porous histoengineering bone rack material, composite heterogene seed cell and / or bioactive factor. The histoengineering bone is constituted through treating heterogene cancellous bone via hypotonic solution and ultrasonic cleaning, complete or partial decalcification, defatting, antigen eliminating, etc to obtain high porosity rack material; compounding mesenchyme stem cell, osteoblast and other seed cell and / or bone morphogonetic protein of the heterogene, vascular endothelial growth factor, antibacterial medicine and other bioactive factors; and applying human serum or no serum culture medium following culturing in ox serum culture medium to reduce heterologous serum residue so as to constitute histoengineering bone product ultimately. The histoengineering bone has high performance and is used as bone repairing material clinically.

The invention discloses 3D bioprinting ink, a preparation method of the ink, a tissue engineering scaffold and a preparation method of the scaffold. The ink contains the following five components: gelatin, sodium alginate, nano-scale magnesium lithium silicate, deionized water and human mesenchymal stem cells. The preparation method of the ink comprises the following steps: firstly dissolving thesterile gelatin and the sodium alginate into the sterile deionized water in order to prepare a mixed prepolymer solution of 140-200 mg / ml gelatin and 20-60 mg / ml sodium alginate; dissolving the sterile magnesium lithium silicate into the sterile deionized water to prepare 20-60 mg / mL magnesium lithium silicate colloid; mixing the two gel in equal volume to prepare a nanocomposite hydrogel which can be used for 3D bioprinting; and finally, uniformly mixing the pre-cultured human mesenchymal stem cells and the nano-composite hydrogel to obtain the nano composite bio-ink with a final cell concentration of 3 x 10<6> / mL. The functionalized, biomimetic tissue engineering bone scaffold with osteogenesis inducing ability is prepared by using the bio-ink as a raw material and adopting a squeeze type 3D bioprinter through printing, and has potential clinical application value

The invention belongs to the field of cartilage tissue repair and tissue engineering and provides a cartilage complex of a tissue engineering bone and a method for preparing the same. The preparation method comprises the following steps of: performing marrow removal, degreasing and layered calcium-removal of cancellous allograft bones or xenogeneic cancellous bones to obtain the cartilage framework of tissue engineering bones, wherein the upper layer of the cartilage framework is completely decalcified and only the surface of the lower layer is decalcified; and using the framework as a primary framework, adding gel-type matters, such as fibrinogens or alginic acid, serving as a secondary framework and a distribution carrier to obtain the cartilage complex of the tissue engineering bone. Compared with the conventional tissue engineering bone cartilage, the cartilage complex of the invention has the advantages of complete natural biomaterials, complete integration, no application of the layered stacking and interface combination technology, high pressure resistant, tensile resistant and shearing resistant properties, uniform and accurate loading of cells, effective enforcement of the differentiation of seeded cells into cartilage cells, easy operation, convenient minimally invasive operation under an arthroscope, and the like.

The invention discloses a double-layer composite scaffold for repairing cartilage in a tissue-engineered bone. The scaffold comprises a cartilage layer and a subchondral layer, wherein the cartilage layer and the subchondral layer are combined into a whole through photopolymerisable modified hyaluronic acid gel, wherein the cartilage layer is made from transformed chondrocyte-loaded photopolymerisable modified hyaluronic acid gel, and the subchondral layer is made of a porous bio-glass scaffold. The invention also discloses a preparation method of the double-layer composite scaffold for repairing cartilage in a tissue-engineered bone, which comprises the following steps: uniformly mixing the transformed chondrocyte-loaded photopolymerisable modified hyaluronic acid gel and in-vitro induced transformed chondrocyte cells, and forming mixed gel through ultraviolet polymerization; putting the porous bio-glass scaffold on the mixed gel, adding the photopolymerisable modified hyaluronic acid gel, and performing light-crosslinking to obtain the double-layer composite scaffold for repairing cartilage in the tissue-engineered bone. The double-layer composite scaffold has excellent biocompatibility and can be degraded, and the preparation method is simple.

The invention discloses a tissue-engineered bone. The tissue-engineered bone is multilayer cell sheet lamination compound with a three-dimensional capillary network, the multilayer cell sheet lamination compound consists of n laminated bone marrow mesenchymal stem cell sheets and vascular endothelial cells among all bone marrow mesenchymal stem cell sheet layers, and n is in a range of 3-8. A preparation method of the tissue-engineered bone comprises steps as follows: firstly, a photosensitive semiconductor structural layer is prepared on the surface of a cell culture dish, so that a photosensitive cell culture dish is obtained; then bone marrow mesenchymal stem cells are cultured with the photosensitive cell culture dish, and the bone marrow mesenchymal stem cell sheets are obtained; finally, the multilayer cell sheet lamination compound consisting of multiple layers of the bone marrow mesenchymal stem cell sheets and the vascular endothelial cells is constructed and cultured to obtain the tissue-engineered bone. The tissue-engineered bone purely consists of homologous cells, so that pollution caused by immunological rejection and stent degradation is reduced, and the tissue-engineered bone is significant in bone defect repair and early vascularization; the method is simple, easy to implement and convenient to popularize.

The invention discloses a tissue engineered bone compound which comprises porous perforated between aperture artificial bone bracket and mesenchymal stem cells implanted in hole and transfected with vascular endothelial growth factor gene. The invention also discloses the application of the tissue engineered bone compound as material for bone defect repair. The inventive tissue engineered bone compound can make seed cell exert osteogenesis, express and secrete VEGF, thereby promoting vasal formation and growth, providing material basis for new bone formation, in favor of neogenesis of vascularized tissue engineered bone tissue, improving effect for repairing bone defect.

A tissue engineered osteochondral cartilage composite stent and a preparation method thereof belong to the technical field of tissue engineering and bio-manufacturing. The preparation method comprises the following steps of: simulating natural materials and structures of osteochondral cartilage; preparing a cartilage stent with an oriented structure by applying the principle of oriented crystallization; preparing a core-spun structural stent by applying a low-temperature deposition manufacturing process based on the rapid forming technique and designing a special extrusion and injection device with a core-spun sprayer; and connecting the oriented structural stent and the core-spun structural stent in a dissolution-adhesion method so as to construct the tissue engineered osteochondral cartilage composite stent with the structure of oriented structural cartilage and core-spun structural bone. According to the tissue engineered osteochondral cartilage composite stent and the preparation method thereof, with respect to the defect treatment of articular cartilages, a material with biological activity is adopted and the osteochondral cartilage composite stent with a special bionic structure is manufactured; the cell implanting density and depth of the stent can be improved, and the simultaneous regeneration of bones and cartilage tissues can be promoted with respect to the different functional regional characteristics of the osteochondral cartilage, so that the repairing effect of osteochondral cartilage defects is improved.

The invention discloses a hydroxyapatite-based biological composite scaffold composed of hydroxyapatite, silk fibroin and chitosan, wherein a mass ratio of the components is represented by that: HA:SF:CS=(60-80):(10-20):(10-20). The composite scaffold is in a porous shape, wherein the porosity is 70 to 90%, and the pore size is 150 to 200mun. The pores are basically round, and are communicated with each other. The invention also discloses a tissue engineered bone constructed by using the hydroxyapatite-based biological composite scaffold. According to the invention, BMSCs transfected with VEGF gene is implanted into the hydroxyapatite-based biological composite scaffold, such that the tissue engineered bone is formed. According to the invention, hydroxyapatite, silk fibroin and chitosanare are adopted as raw materials, and a porogen-leaching technology is combined with a vacuum drying technology, such that the hydroxyapatite-based biological composite scaffold material is prepared. A degradation rate of the scaffold is regulated through the regulation of the proportions of the components. The pore size distribution of the composite material is controlled through the regulations of the radiuses and the addition amount of sodium chloride particles.

The invention provides a tissue engineered bone-cartilage composite tissue transplant and a preparation method thereof, and belongs to the tissue engineering field. The method comprises the following steps: seed cells are implanted on a scaffold material and cultured in vitro to form the tissue engineered bone-cartilage composite tissue transplant; the seed cells are osteogenic stem cells; the scaffold material comprises an osteogenic part and a chondroblast part, and the osteogenic part is added with an osteoblast induction factor and modified by a type-I collagen in a preparation process; and the chondroblast part is added with a chondroblast induction factor and modified by a type-II collagen. Bone marrow mesenchymal stem cells are successfully induced into osteocytes and chondrocytes, and bone tissues and cartilage tissues are formed after the obtained transplant is transplanted in vivo. Satisfactory effect is obtained from the tissue engineered bone-cartilage composite tissue which is constructed by the transplant.

The invention relates to a bone matrix material containing various proteins secreted by umbilical cord mesenchymal stem cells and a preparation method thereof. The material is an animal bone matrix material with the umbilical cord mesenchymal stem cells inoculated on the surface of a decalcified bone matrix and used for secreting osteogenic induction function proteins to promote osteogenesis. The bone matrix material has natural structures and characteristics of bone tissues and a good osteogenic induction effect, has a better osteogenesis promoting effect in a bone defect repair process, and is helpful for large-scale preparation and clinical application of tissue-engineered bones.

A cell-removed heterogeneous bone matrix is prepared from the rib and extremity bone of pig through physical and chemical processing, and removing cells and the heterogeneous protein from tissue by use of proteolytic enzyme and Triton-X100. Its advantages are natural components and biologic chemical strength, low antigenicity, high compatibility and bone conductivity, and low cost.

The invention discloses a bone-defect regeneration and repair tissue-engineered bone, as well as a construction method and an application thereof. The tissue-engineered bone is a composite laminated structural body formed by three layers of mesenchymal stem cell polymers and two layers of bone matrix particles, wherein the composite laminated structural body is a complex formed by mesenchymal stem cell polymers and bone matrix particles which are alternately arranged; the mesenchymal stem cell polymer comprises mesenchymal stem cells and extracellular matrix which is secreted by the mesenchymal stem cells and is distributed around the mesenchymal stem cells. The tissue-engineered bone formed by adopting the construction method can be used for simultaneously compounding living cells and active factors, the repairing and reconstructing process is quick, the joint of new bone and surrounding autogenous bone is complete and uniform in thickness. The tissue-engineered bone is suitable for bone-defect regeneration and repair, physiological regeneration of bones is realized, and the repairing treatment effect of bone defect is improved.

The present invention discloses one kind of injected tissue engineering bone and its construction and application. The injected tissue engineering bone consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg / ml concentration fibrinogen solution in the ratio of 1 to 3-6, and contains marrow matrix stem cell in the concentration of 5*10<>6< / >-10*10<>7< / > / ml; and the solution II is solution mixture of calcium chloride in 40 mmol / ml concentration and thrombin in 400-1000 U / ml concentration. When the injected tissue engineering bone is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected tissue engineering bone is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

A tissue-engineered bone-cartilage assembly for repairing the damaged bone and cartilage by inserting it in the damaged position is prepared through preparing bone and cartilage by tissue engineering, adhering them together, and making a wedge on bone part.

The present invention relates to a method capable of utilizing internal perfusion to induce bone marrow matrix stem cell to construct tissue engineering bone. Said method includes the following steps: (1), extraction, separation and culture of bone marrow matrix stem cell; (2), preparation of coral composite artificial bone; and (3), creation of perfusion system.

Method and apparatus for preparing bone and cartilage transplants for the reconstruction of the acetabulum, femoral head, or both with tissue engineered osteochondral constructs or and osteochondral allograft transplant.

The invention discloses a polydopamine-modified chitosan-based material and a preparation method thereof. The preparation method comprises the following concrete steps: (1) preparing a chitosan solution with a certain concentration from acetic acid, carrying out crosslinking by using glutaraldehyde as a cross-linking agent and casting the cross-linked chitosan solution into a die; (2) subjecting the chitosan solution obtained in the step (1) to gelating at first and natural air drying next so as to obtain a chitosan film; (3) dissolving dopamine in a buffer solution of tris(hydroxymethyl)aminomethane hydrochloride and adjusting the pH value of the obtained solution to 8.5 so as to obtain a polydopamine solution with a certain concentration; and (4) soaking the chitosan film prepared in the step (1) in the polydopamine solution obtained in the step (3), then taking the material out for cleaning and carrying out air drying again so as to obtain the surface polydopamine-modified chitosan film material. The method provided by the invention can improve surface hydrophilicity of the chitosan material, promotes deposition of a phosphate component on the surface of the material, provides a better coating material for a tissue-engineered bone repair scaffold, is simple in process and has good application prospects and scientific significance.

The invention discloses an injectable strengthened phosphate lime / hydrogel microcapsule tissue engineering bone which is prepared from the following components in percentage by weight: 20-75% of calcium phosphate cement, 20-75% of chitosan, 1-10% of PLGA electric spin and 3-155 of hUCMSCs hydrogel microcapsule. The invention also discloses a preparation method of the tissue engineering bone and application of the tissue engineering bone as a bone defect repairing material. The tissue engineering bone not only maintains original good biocompatibility and osteogenesis inducing property of phosphate lime, but also obviously improved mechanical properties and capability for carrying seed cells. The tissue engineering bone can be used for treating bone defect or bone deficiency caused by various diseases and has the characteristics of easy operation, convenient molding, maximum wound reduction, operation difficulty reduction, patient pain alleviation, less infection danger, less scar formation and the like.