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Dual-layer composite scaffold for repairing cartilage of tissue engineered bone and preparation method thereof

A tissue engineering bone and cartilage repair technology, applied in medical science, prosthesis, etc., can solve the problems of difficult degradation and poor biocompatibility, and achieve the effect of simple method, good biocompatibility and good osteoconductivity

Inactive Publication Date: 2014-06-25
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the defects of poor biocompatibility and difficult degradation of cartilage repair materials in the prior art, the purpose of the present invention is to provide a double-layer composite scaffold for tissue engineering osteochondral repair; it has good biocompatibility, and the modified Hyaluronic acid is photopolymerizable, and can be degraded under the action of hyaluronan decomposing enzymes in vivo, and the degradation products are not biologically toxic

Method used

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  • Dual-layer composite scaffold for repairing cartilage of tissue engineered bone and preparation method thereof
  • Dual-layer composite scaffold for repairing cartilage of tissue engineered bone and preparation method thereof
  • Dual-layer composite scaffold for repairing cartilage of tissue engineered bone and preparation method thereof

Examples

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preparation example Construction

[0036] In the following examples, the preparation method of photopolymerized modified hyaluronic acid gel comprises the following steps:

[0037] An aqueous solution of sodium hyaluronate (molecular weight: 66-90 kilodaltons) with a mass concentration of 1-5% is stirred and dissolved at the freezing point, and then mixed with methacrylic anhydride. The concentration of methacrylic anhydride is hyaluronic acid 20 times the molar concentration of sodium bicarbonate, adjust the pH so that the pH value is always greater than 8, stir and react at freezing point for 24 hours, put it into the dialysis membrane and dialyze with 50 times the volume of deionized water for 3 days, freeze-dry for 48 hours, and then mix with normal saline and The cross-linking agent I2959 was mixed to obtain a photopolymerized modified hyaluronic acid gel; the concentration of the cross-linking agent I2959 was 0.05wt%.

Embodiment 1

[0039] The preparation method of the double-layer composite scaffold for tissue engineering osteochondral repair comprises the following steps: filter and sterilize the modified hyaluronic acid gel with photopolymerization through a 0.02 μm filter membrane, and induce and transform chondrocytes from bone marrow mesenchymal stem cells in vitro After mixing evenly, make the cell concentration 40×10 6 / ml, the hydrogel complex with a mass concentration of modified sodium hyaluronate of 1% and a mass concentration of I2959 of 0.05% is put into a mold with a diameter of 5mm and a height of 5mm, and is passed through a distance of an ultraviolet lamp with a wavelength of 320-400nm After irradiating at 15cm for 10 minutes, cross-linking forms a hybrid gel; place the porous silicate bioglass scaffold on the hybrid gel, add 50 μL of modified hyaluronic acid gel with photopolymerization, and UV light with a wavelength of 320-400nm A double-layer composite scaffold for tissue engineering...

Embodiment 2

[0042] The preparation method of the double-layer composite scaffold for tissue engineering osteochondral repair includes the following steps: sterilize the modified hyaluronic acid gel with photopolymerization in an ultra-clean bench for half an hour by ultraviolet light, and then dissolve it in sterile PBS containing I2959 solution, and mixed with adipose-derived stem cells in vitro induced transformation of chondrocytes to make a cell concentration of 5 × 10 6 / ml, the hydrogel complex with a mass concentration of sodium hyaluronate of 2% and a mass concentration of I2959 of 0.05% is placed in a mold with a diameter of 5mm and a height of 5mm, and is placed at a distance of 25cm by an ultraviolet lamp with a wavelength of 320-400nm. After irradiating for 15 minutes, cross-link to form a mixed gel; place the porous borosilicate bioglass scaffold on the mixed gel, add 30 μL of modified hyaluronic acid gel with photopolymerization, and use a UV lamp with a wavelength of 320-400...

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Abstract

The invention discloses a double-layer composite scaffold for repairing cartilage in a tissue-engineered bone. The scaffold comprises a cartilage layer and a subchondral layer, wherein the cartilage layer and the subchondral layer are combined into a whole through photopolymerisable modified hyaluronic acid gel, wherein the cartilage layer is made from transformed chondrocyte-loaded photopolymerisable modified hyaluronic acid gel, and the subchondral layer is made of a porous bio-glass scaffold. The invention also discloses a preparation method of the double-layer composite scaffold for repairing cartilage in a tissue-engineered bone, which comprises the following steps: uniformly mixing the transformed chondrocyte-loaded photopolymerisable modified hyaluronic acid gel and in-vitro induced transformed chondrocyte cells, and forming mixed gel through ultraviolet polymerization; putting the porous bio-glass scaffold on the mixed gel, adding the photopolymerisable modified hyaluronic acid gel, and performing light-crosslinking to obtain the double-layer composite scaffold for repairing cartilage in the tissue-engineered bone. The double-layer composite scaffold has excellent biocompatibility and can be degraded, and the preparation method is simple.

Description

technical field [0001] The invention belongs to the technical field of osteochondral tissue engineering scaffolds, in particular to a scaffold structure for osteochondral repair and a preparation method thereof. Background technique [0002] Articular cartilage defects are present in 61% of adult knees; of these, 19% are osteochondral defects. Articular cartilage has a limited ability to repair itself, and it is difficult to recover once damaged. Current conventional treatment methods include autologous cartilage transplantation, autologous or allogeneic osteochondral transplantation. These treatments have certain limitations, such as limited sources of cartilage grafts, impaired donor site function, immune rejection, and implant loosening. With the advent of tissue engineering technology, the research on osteochondral repair has been greatly developed. The construction of osteochondral tissue engineering scaffolds requires cells, cartilage extracellular matrix and subcho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/38A61L27/52A61L27/56A61L27/10
Inventor 黄文旵顾刈非周萘王德平
Owner TONGJI UNIV
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