Tissue engineering bone/cartilage double-layer scaffold and construction method and application thereof

Abstract

The invention discloses a tissue engineering bone / cartilage double-layer scaffold and a construction method and application in medical science. The method adopts natural cartilage and bone as materials, respectively removes the antigenicity of the cartilage and the bone through de-cell, and adopts a freezing and drying method to prepare the double-layer scaffold simultaneously suitable for growthof the two issues of the bone and the cartilage. A preparation method comprises that: acellular bone is soaked in suspension of cartilage acellular matrix microfilaments, and the double-layer scaffoldof which the upper layer is a cartilage acellular matrix three-dimensional porous spongy scaffold and the lower layer is an acellular bone scaffold is prepared by the freezing and drying method and acrosslinking method. The cartilage acellular matrix spongy scaffold part of the double-layer scaffold can be introduced with factors promoting the formation of the cartilage, and the acellular bone part can be introduced with factors promoting the formation of the bone, and the factors can be respectively inoculated with cells for forming the cartilage or the bone to construct a living tissue engineering bone / cartilage complex, which is used for repairing bone and cartilage complex defect or total joint defect clinically.

Description

technical field [0001] The present invention relates to a double-layer scaffold suitable for the growth of bone and cartilage, in particular to a histocompatible double-layer scaffold for guiding bone and cartilage composite tissue regeneration and its construction method. The present invention also relates to the double-layer scaffold The use of the bracket in medicine belongs to the field of tissue engineering. Background technique [0002] Tissue engineering is an interdisciplinary subject involving cell biology, material science, engineering and bioreactors. It uses the basic principles and methods of life science and engineering to construct the tissues needed by the human body for repairing and replacing trauma, Diseased non-functioning tissue (organ). The main content of tissue engineering research includes: seed cells, scaffold materials, growth factors. Seed cells include chondrocytes, bone marrow stromal stem cells, adipose-derived stem cells, and genetically eng...

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Problems solved by technology

Assembly methods include fibrin gel bonding, atraumatic suture suture method, or polylactic acid stick bonding fixation, etc., but the bone-cartilage complex constructed has the problem of poor bone-cartilage interface integration (Schaefer D, Martin I, Shastri P, et al. In vitro generation of osteochondralcomposites. Biomaterials JT. Biomaterials, 2000, 21: 2599-606)

[0003] Recently foreign countries have used cancellous bone and agarose gel wrapped in chondrocytes to culture bone and cartilage complexes in vitro. Using the characteristics of agarose gel that can penetrate into cancellous bone, the tissue engineered cartilage part and bone scaffold can achieve a good combination, but The mechanical properties of the gel are poor and cannot meet the requirements of in vivo load-bearing (Hung CT, Lima EG, Mauck RL, et al. Anatomically shaped osteochondral constructs for articular cartilage repair. J Biomech JT -Journal of biomechanics, 2003, 36: 1853- 64)

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