Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue engineering bone and its construction and application

A technology of tissue engineering bone and construction method, which is applied in bone diseases, drug combinations, medical preparations containing active ingredients, etc. It can solve the problems of imperfect products, no obvious effect of bone defect repair, and difficulty in inducing activity to be fully exerted, etc. problems, to achieve the effect of easy processing and good biocompatibility

Inactive Publication Date: 2006-10-18
NAN FANG HOSPITAL
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because BMP has very little content in bone tissue (about 1 mg / kg wet bone), and diffuses quickly in the body, it is easily decomposed by proteases, so it cannot continuously stimulate and induce osteogenesis in the local area. hard to get the most out of
[0006] Although great progress has been made in the development of tissue engineered bone, and some products have entered the stage of clinical trials, these products are still not perfect, and have defects in many aspects such as mature methods, development cycle and product cost, especially for large bone defects in clinical practice. Repair still has no obvious effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue engineering bone and its construction and application
  • Tissue engineering bone and its construction and application
  • Tissue engineering bone and its construction and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: In vitro culture, expansion and directional induction of bone marrow stromal stem cells

[0042]According to the whole bone marrow cell culture method, the bone marrow puncture needle was inserted into the bone marrow cavity of the ilium, a total of 3-5ml of bilateral bone marrow was aspirated, mixed into DMEM medium, anticoagulated with 500U / ml heparin solution, mixed and then centrifuged at 800r / min at low speed for 5min , remove the supernatant, resuspend the culture medium and inoculate through a 90-mesh filter. At 37°C, 5% CO 2 Cultivate in an incubator, change the medium in half (DMEM complete medium, 15% serum) after 4 days, change the medium once in the next 2 to 3 days, observe daily with an inverted microscope, digest with 0.25% trypsin after the cells confluent into a monolayer, Counting, subculturing, and routinely changing the medium once every 3 days. The 3rd generation cells were transferred to DMEM conditioned medium (containing 15% serum, 5...

Embodiment 2

[0043] Example 2: Loading of PLGA scaffolds and cytokines

[0044] (1) Using modified PLGA with large pore size and high porosity as raw material, adding powdered cytokine bone morphogenetic protein, namely BMP, and trimming into shape;

[0045] (2) Ultrasonic vibration cleaning: use triple distilled water to clean with ultrasonic vibration, and dry naturally

[0046] (3) Surface deacidification treatment: immerse in 1% NaOH sequentially 2 2 hours, PBS buffer washing, 50% ethanol 2 hours, PBS buffer washing;

[0047] (4) Sterilize by ethylene oxide fumigation at 37°C;

[0048] (5) Add DMEM complete medium containing 15% serum and soak for 2-3 days;

[0049] (6) Add 10% polylysine solution and soak for 1-2 days;

[0050] (7) Dry under ultraviolet light at 15-25°C and store for future use.

[0051] In this embodiment, the PLGA scaffold is in the shape of a 4×4×2 mm block, with a pore diameter of 150-200 um and a pore diameter ratio of 85-95%.

Embodiment 3

[0052] Example 3: Construction of Tissue Engineered Bone Loaded with Cytokines

[0053] According to the method described in Example 1, the BMSCs that had passed the third generation and had grown well after being induced by the conditioned medium were digested with trypsin, centrifuged, and washed with 1×10 6 -1×10 7 / ml concentration Use a micropipette to inoculate the cell suspension onto the material constructed in Example 2, and place it at 37°C, 5% CO 2 Cultivate in the incubator for 4 hours, add an appropriate amount of conditioned medium, put it back into the incubator and continue to culture for 3-5 days, and then obtain the tissue-engineered bone and save it for future use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to View More

Abstract

The present invention belongs to the field of artificial organ constructing technology in medicine engineering, and is especially one kind of tissue engineering bone and its construction and application. The tissue engineering bone of the present invention includes carrier rack and seed cell attached to the carrier rack to form complex with the 3D structure and bioactivity of bone tissue. The carrier rack is modified PLGA through deacidification treatment to have great pores and high porosity, and has cell factor bone morphogenesis protein (PMP) carried; and the seed cell is marrow matrix stem cell. The tissue engineering bone of the present invention may be used in constructing functional tissue engineering bone graft for repairing large bone defect.

Description

technical field [0001] The invention belongs to the technical field of constructing artificial organs by tissue engineering method in biomedical engineering, and in particular relates to a tissue engineering bone and its construction and application. Background technique [0002] In recent years, with the development of tissue engineering technology, reports on the research and development of biological substitutes that can repair, maintain or improve the function of damaged tissues have gradually increased, among which bone tissue engineering research is a hot spot, and a relatively complete system has gradually formed. Theories and technical routes on seed cells, cell carrier scaffolds and tissue construction. But it is undeniable that these achievements are still far from the final clinical application of bone tissue engineering. Therefore, it is necessary to develop a new type of tissue-engineered bone product that can be rapidly applied in clinic. [0003] The basic m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/00A61K38/19A61L27/56A61P19/08
Inventor 裴国献胡稷杰金丹魏宽海黄爱文张元平
Owner NAN FANG HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com