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Artificial bone and its making method

A manufacturing method and artificial bone technology, applied in the field of bone substitute materials, can solve problems such as long-term retention of foreign bodies, rejection reactions, chronic inflammatory reactions of degradation products, etc., and achieve the effect of reducing healing and using safely

Inactive Publication Date: 2008-04-30
XIAN REJE BIOLOGICAL TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide an artificial bone and its manufacturing method, which solves the problem of chronic inflammatory response or rejection caused by the presence of scaffolds in bone substitute materials in the background technology, or the long-term retention of foreign bodies, or degradation products. question

Method used

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Examples

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Effect test

experiment example 1

[0044] (1) Extract 3ml of bone marrow from the iliac crest under sterile conditions and anticoagulate with heparin.

[0045] (2) Transfer bone marrow to 75cm 2In the culture flask, the bone marrow stromal stem cells are cultured and expanded with a culture medium having an inducing effect. The culture medium with inductive effect is DMEM, which contains 10% serum, 50 μg / ml vitamin C, 10 -8 M dexamethasone, 10 mM sodium β-glycerophosphate.

[0046] (3) Continuous culture and expansion, and the culture medium with induction effect is replaced every 3-4 days. Observed with an inverted microscope, until the induced bone marrow stromal stem cells contact each other, connect into a sheet, the outline disappears, and mineralized nodules appear. Observation picture under X100 inverted microscope, see figure 1 . Use a spatula to lift it from the bottom of the flask to obtain a cell sheet, see figure 2 .

[0047] (4) The cell membrane is wrapped on the forming mold surface of a ...

experiment example 2

[0052] When the bone tissue to be implanted is not tubular, the cell membrane sheet formed in Experimental Example 1 is directly folded into a square shape of about 10×10×2 mm to form the embryonic form of the artificial bone. Continue to culture in vitro for 4 weeks to form a square-shaped artificial bone.

[0053] Experimental results: Implanted into the subcutaneous tissue of nude mice under aseptic conditions, mature bone tissue was formed after 2 months, as observed under the X100 microscope, see Figure 7 .

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Abstract

Disclosed are an artificial bone and a manufacturing method of the artificial bone. Cell membranes form a prototype of the artificial bone, the cell membranes are cultivated in culture fluid of inducing action, and then the cell membranes are mutually engaged, finally the artificial bone is achieved. The composition of the artificial bone comprises bone marrow stromal cells after being induced and stromas which are secreted by the cells, histology represents a chondroid tissue, the coloration of safranine -O shows a positive reaction, and when the artificial bone is implanted into a hypoderm of a naked mouse, a mature bone tissue can be formed. The invention solves technical problems that alternate materials of bones of the background technique produce chronic inflammation reaction or rejection owning to the existing of a support, the long term remaining of foreign matters, or the degrading of products. According to the sizes and shapes of damaged bones, a reproduced bone tissue without supports are a complete biological tissue of the functions of safe and reliable usage and being capable of completely replacing damaged bones.

Description

technical field [0001] The invention relates to a bone substitute material, in particular to an artificial bone completely composed of cells and matrix secreted by cells and a manufacturing method thereof. Background technique [0002] Contemporary medicine has no effective treatment methods for bone defects and nonunions caused by various reasons such as trauma and bone tumor lesions. It mainly relies on surgical fixation and connection, or the use of bone graft materials, autologous bone, and xenograft bone transplantation. Due to the long healing period, it is easy to lead to various complications, many of which are disabled due to the lack of effective treatment. [0003] Tissue engineering is a new discipline developed in recent years. The basic idea is: separate and cultivate cells in vitro, plant cells on a three-dimensional scaffold with a certain spatial structure, and form cells with a certain structure and function through mutual adhesion, growth and reproduction...

Claims

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Application Information

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IPC IPC(8): A61L27/38
Inventor 陈富林
Owner XIAN REJE BIOLOGICAL TECH
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