Method for rapidly filtering and screening medulla ossium substrate stem cell

Abstract

The invention relates to a filtering method, in particular to a method for quickly filtering and screening bone marrow stromal cells. The method adopts the technical proposal that the method for quickly filtering and screening the bone marrow stromal cells comprises the following steps: firstly, performing anticoagulant treatment on bone marrow blood to be used; secondly, filtering the bone marrow blood after anticoagulant treatment through a filtration recombiner; and thirdly, collecting stem cell-frame composite materials. The method has the advantages that the method quickly recombines the cells and a vector (namely a filter screen material) when filtering and screening the bone marrow stromal cells simultaneously, so as to integrate the two procedures of cell screening and cell-vector combination into a whole. The filtering method has short time consumption, does not need in vitro culture, can be simultaneously performed in an operation, and is more favorable for clinic use because the volume of the screened stromal cells is smaller than that of cell suspension finally obtained by density gradient centrifugation technology.

Description

technical field [0001] The invention relates to a filtering method, in particular to a method for rapidly filtering and screening bone marrow stromal stem cells. Background technique [0002] The repair of bone defects often requires bone grafting or bone substitute materials to fill, and the current research hotspot in tissue engineering in this field is the scaffold carrier compounded with stem cells. Bone marrow stromal stem cells can be isolated by adherent culture or density gradient centrifugation, and then expanded in vitro. Although bone marrow stromal stem cells can be isolated in vitro and scaffolds containing cells can be prepared, they are still in the laboratory research stage and need to be implanted. For the human body, there are still many technical and ethical problems, and the cultivation cycle is long, easy to be polluted, and the cost is high, which affects the clinical application and promotion. [0003] At present, the cell screening techniques mainly ...

AI Technical Summary

Problems solved by technology

[0004] 1) Although the separation method of flow cytometry separates cells quickly and has high purity, it is expensive, and it is difficult to separate trace cells with too low content, and it cannot handle a large number of cell samples, and it is also difficult to obtain a sterile cell suspension

2) The immunomagnetic bead separation method has a great impact on the activity of cells, and the recovery rate is only 60%-75%, which may lead to complete inactivation of cells. The same operation is more complicated and expensive

However, it takes a long time, involves in vitro culture of cells, and is difficult for clinical use.

4) Density gradient centrifugation is a commonly used method for concentrating and separating cells, but it often requires the use of a separation medium (such as Ficoll or Hypaque solution, etc.). Washing away the separation medium, maintaining the sterility of the entire operation requires special equipment, which is expensive

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