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Non-viruse gene transferring vector and its preparation and application thereof

A gene transfer, non-viral technology, applied in the field of life sciences, can solve the problems of not considering the target specificity, not considering the prospect, etc., to achieve the effect of mass production, high transfection efficiency and small molecular weight

Inactive Publication Date: 2006-06-28
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
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Problems solved by technology

In recent years, people have developed a variety of ligand-polymer gene transfer systems, including a series of RGD peptides designed by Hart SL [Hart SL, Harbottle RP, Cooper R, et al, Gene delivery and expression mediated by an integrin-binding peptide. Gene Ther 1995; 2: 552; HarbottleRP, Cooper RG, Hart SL, et al.An RGD oligolysine peptide: a prototype construct for integrin-mediated gene delivery. Hum Gene Ther1998; 9: 1037], but these vectors have not considered the The target specificity of bone tissue engineering seed cells-bone marrow stromal stem cells when exogenous gene transfection is not taken into account the prospect of application in bone tissue engineering research

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  • Non-viruse gene transferring vector and its preparation and application thereof
  • Non-viruse gene transferring vector and its preparation and application thereof
  • Non-viruse gene transferring vector and its preparation and application thereof

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Embodiment Construction

[0021] The implementation of the present invention is described in detail below in conjunction with accompanying drawing:

[0022] The method for preparing a non-viral gene transfer vector of the present invention comprises the following steps; using FMOC / tBu solid-phase polypeptide synthesis method to connect the first amino acid-cysteine ​​to the resin, and remove the first amino acid-cysteine The Fmoc protecting group of the acid, and then proline, repeat the above steps until all the SDGRG and 16 lysines are connected to generate the amino terminal. After the short peptide is synthesized, the peptide chain is cut from the resin with a trifluoroacetic acid solution Next, the peptides were dissolved in TFA and filtered through a gel filtration column, followed by reverse phase C 18 The analytical column was filtered and then lyophilized into a powder.

[0023] The structural sequence of a non-viral gene transfer vector of the present invention is: NH2-lys-lys-lys-lys-lys-ly...

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Abstract

The invention relates to non-virus gene transfer vector. Its structure is as follows: NH2-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-lys-Gly-Arg-Gly-Asp-Ser-Pro-Cys-COOH. The invention has good target direction property for non virus gene transfer vector, high transfection efficiency, and can linkage with bone matrix material to increase cell specificity adherence and form gene carrying material to realize cell directional transfection. The invention also discloses the non-virus gene transfer vector manufacturing method and its application in medicine experimental study and clinic gene therapy.

Description

technical field [0001] The invention belongs to the field of life sciences, and more specifically relates to a non-viral gene transfection carrier and its preparation and application in medical experiments and clinical gene therapy. Background technique [0002] Gene transfer vectors include viral vectors and non-viral vectors. Compared with viral vectors, non-viral vectors have the advantages of easy preparation, large capacity for carrying foreign genes, non-immunogenicity, safety, and economy, and thus become an important research field of gene transfer technology in molecular biology and tissue engineering. research direction. [0003] There are currently two research hotspots on improving non-viral gene transfer vectors. One of the research hotspots is the Gene Activation Matrix (GAM). The method is to coat the target gene on the tissue engineering matrix material with a three-dimensional porous structure. This gene-carrying matrix material can provide sufficient nutr...

Claims

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Application Information

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IPC IPC(8): C12N15/63A61K48/00C12N15/87
Inventor 郑启新郭晓东潘海涛吴永超刘勇宋玉林陈顺广
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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