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Bone cartilage repair material and preparation method of scaffold for tissue engineering

A tissue engineering scaffold and repair material technology, applied in the field of regenerative medicine and biomaterials, can solve the problems of unestablished osteochondral regeneration strategy and the complexity of osteochondral tissue, and achieve the effect of promoting osteogenic differentiation and simple preparation

Inactive Publication Date: 2019-02-22
王翀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, due to the considerable complexity of osteochondral tissue, effective strategies for osteochondral regeneration have not yet been established

Method used

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  • Bone cartilage repair material and preparation method of scaffold for tissue engineering
  • Bone cartilage repair material and preparation method of scaffold for tissue engineering
  • Bone cartilage repair material and preparation method of scaffold for tissue engineering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] An osteochondral repair material, the material includes a subchondral bone layer, an interface layer and a cartilage layer arranged in sequence, the interface layer is located between the subchondral bone layer and the cartilage layer, and the material includes the following components in parts by weight : 10 parts of oil-soluble polymer material, 10 parts of bioceramic powder, 50 parts of oil solvent, 0.1 part of water-soluble bioactive material, 2 parts of water, 0.2 part of emulsifier, 10 parts of hydrogel material, 1 part of seed cell and 50 copies of culture medium;

[0043] The oil-soluble polymer material used in the subchondral bone layer is racemic polylactic acid PDLLA; the oil-soluble polymer material used in the cartilage layer is a heat-responsive shape-memory polymer, and the heat-responsive shape-memory polymer is specifically polylactic acid-Sanya Methyl carbonate PLA-PTMC, where LA:TMC is 60:40-80:20. The oily solvent is specifically dichloromethane; t...

Embodiment 2

[0054] An osteochondral repair material, the material includes a subchondral bone layer, an interface layer and a cartilage layer arranged in sequence, the interface layer is located between the subchondral bone layer and the cartilage layer, and the material includes the following components in parts by weight : 25 parts of oil-soluble polymer material, 30 parts of bioceramic powder, 80 parts of oil solvent, 0.6 part of water-soluble bioactive material, 20 parts of water, 0.6 part of emulsifier, 20 parts of hydrogel material, 6 parts of seed cells and 70 copies of culture medium;

[0055] The oil-soluble polymer material used in the subchondral bone layer is lactic acid-glycolic acid copolymer PLGA; the oil-soluble polymer material used in the cartilage layer is a heat-responsive shape-memory polymer, and the heat-responsive shape-memory polymer is specifically polyethylene glycol Lactone PCL. The oily solvent is dichloromethane; the bioceramic is beta-tricalcium phosphate; ...

Embodiment 3

[0066] An osteochondral repair material, the material includes a subchondral bone layer, an interface layer and a cartilage layer arranged in sequence, the interface layer is located between the subchondral bone layer and the cartilage layer, and the material includes the following components in parts by weight : 40 parts of oil-soluble polymer material, 50 parts of bioceramic powder, 100 parts of oily solvent, 1 part of water-soluble bioactive material, 30 parts of water, 1 part of emulsifier, 30 parts of hydrogel material, 10 parts of seed cells and 100 copies of culture medium.

[0067] The oil-soluble polymer material used in the subchondral bone layer is poly-β-hydroxybutyric acid (PHB); the oil-soluble polymer material used in the cartilage layer is a heat-responsive shape-memory polymer, and the heat-responsive shape-memory polymer specifically It is polylactic acid-trimethylene carbonate PLA-PTMC, wherein the ratio of LA:TMC is 80:20~95:5. The oily solvent is specific...

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Abstract

The invention discloses a bone cartilage repair material and a preparation method of a scaffold for tissue engineering. The material comprises a subchondral bone layer, an interface layer and a cartilage layer arranged in sequence, wherein the interface layer is positioned between the subchondral bone layer and the cartilage layer; the material contains the following components in parts by weight:10-40 parts of oil-soluble high polymer materials, 10-50 parts of biological ceramic powder, 50-100 parts of oily solvents, 0.1-1 part of a water-soluble bio-active material, 2-30 parts of water, more than 0 and less than 1 part of an emulsifier, 10-30 parts of a hydrogel material, 0-10 parts of seed cells and 50-100 parts of a culture medium. The preparation method comprises the following steps:preparing printing ink according to the ratio of the materials, and transferring into 3D printing equipment for printing molding. The bone cartilage scaffold having a personalized appearance can be prepared, load and controlled release of different growth factors or peptides in different zones of the scaffold can be realized, and directional differentiation of bone marrow stem cells at differentparts to osteoblast and chondrocyte can be promoted.

Description

technical field [0001] The invention belongs to the technical fields of biomaterials and regenerative medicine, and in particular relates to a printing material used for printing a tissue engineering scaffold for osteochondral repair, and an osteochondral tissue engineering scaffold prepared by using the material. Background technique [0002] The aggravation of population aging and frequent occurrence of sports injuries have caused more and more people to face degenerative and traumatic articular cartilage injuries. According to statistics, there are more than 10 million new cases of cartilage injury in my country every year. Articular cartilage includes subchondral bone, bone-cartilage interface, and cartilage. Severe joint damage can easily develop into osteoarthritis. Studies have found that in the early stage of osteoarthritis, the structure and morphology of subchondral bone have changed. As an important tissue supporting cartilage metabolism and mechanical shock ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/12A61L27/18A61L27/20A61L27/22A61L27/38A61L27/54A61L27/56B33Y80/00
CPCA61L27/12A61L27/18A61L27/20A61L27/222A61L27/227A61L27/3817A61L27/3821A61L27/3834A61L27/3852A61L27/54A61L27/56A61L2300/252A61L2300/412A61L2300/414A61L2430/06B33Y80/00C08L67/04C08L5/04C08L5/16C08L89/00
Inventor 王翀危岩万健徐若齐童庆谢登辉朱煌荣钟凯宏王敏
Owner 王翀
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