158 results about "Cartilage injury" patented technology

Methods and devices for the regulation of type II collagen gene expression in cartilage cells via the application of specific and selective fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articular cartilage. By gene expression is meant the up regulation or down regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased cartilage tissue that include generating specific and selective electric and electromagnetic signals that generate specific and selective fields optimized for type II collagen gene expression and exposing cartilage tissue to the specific and selective fields generated by specific and selective signals so as to regulate type II collagen gene expression in such cartilage tissue. The resulting methods and devices are useful for the targeted treatment of osteoarthritis, rheumatoid arthritis, cartilage injury, and cartilage defects.

Various embodiments provide systems and methods of treating damaged cartilage injuries to joints and connective tissue. Methods and systems useful for permanent relief of pain in joints are also provided herein. Various embodiments provide for combining therapeutic ultrasound energy directed to a joint with a medicant injected into the joint.

An improved cold / compress therapy for the treatment of muscle, tissue, tendon, or cartilage injury or damage. The ICE Power Pack consists of a plurality of hollow vessels filled with water or aqueous solutions or colloids that can be solidified in a conventional domestic or commercial freezer. The encapsulated water or aqueous solutions or colloids rely on the high latent heat of fusion as they undergo a phase change providing an extended therapeutic period in a medically safe temperature zone. The vessels are sandwiched between flexible insulating and non-insulating layers that serve as compresses.

The invention discloses a polysaccharide-dopamine composite biogel and application of the biogel. A preparation method of the biogel comprises the following steps: dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide sodium in an MES (Methyl Ester Sulfonate) buffer solution with a pH value being 5-6 in an inert gas atmosphere so as to prepare an EDC (ethylcarbodiimide) mixed solution, subsequently adding the EDC mixed solution into a polysaccharide solution for reacting at 20-30 DEG C for 0.5-1 hour, then adding a dopamine solution for further reacting at 20-30 DEG C for 1-4 hours, dialyzing a reactant by taking distilled water as a dialyzate, taking trapped fluid for freezing and drying to obtain the polysaccharide-dopamine composite biogel. The polysaccharide-dopamine composite biogel is simple and effective in modification method and capable of increasing the surface viscidity of the cartilage tissue, reducing loss of therapeutic drugs or implanted cells and facilitating the repairing of the cartilage injury.

The invention relates to a cross-linked hyaluronic acid cell-scaffold material and its preparation method and application. The cross-linked hyaluronic acid cell-scaffold material is obtained by crosslinking of high-molecular hyaluronate and low-molecular hyaluronate. The proportion of hyaluronate disaccharide molecules which take part in the crosslinking is 0.5%-20%, and expansion rate of the hyaluronate disaccharide molecules in an isotonic solution is 80%-110%. The preparation method of the cell-scaffold material comprises two freeze drying steps. Hyaluronate mixed with a cross-linking agent is formed firstly; after a heating reaction, water is added for swelling to form gel; and freeze drying is then carried out to obtain the porous scaffold material. The scaffold has abundant pores, has a certain mechanical strength and pore size and has good hydroscopicity and biocompatibility. The scaffold can be used as a tissue engineering cell-scaffold in promoting cartilage injury repairing and also can be used for preparing a haemostasis anti-adhesion material.

The invention relates to a preparation method of a tissue repair material. The preparation method comprises the following steps: carrying out pre-treatment; vibrating in an organic solvent to degrease; deactivating a virus with an ethanol solution; removing cells by high and low osmotic solutions; swelling in an acidic or alkaline liquid; and quickly lyophilizing and sterilizing. The thickness of the tissue repair material is increased by 2-4 times, and the material is loose and porous, small in change of mechanical property and degradation property and good in cell removal effect and has relatively high biosafety and biocompatibility. Due to the structural characteristics of proper thickness and looseness and porosity, the material is suitable for damage repair for filling damaged parts required by repair such as cartilage injury, skin and soft tissue defect, gingival recession and the like. Animal experiments verify that the repaired region after eight weeks has no depressed phenomena and is fully replaced by regenerated tissues. Moreover, the regenerated tissue and autologous tissue are healed well and are free of separating phenomenon, which verifies that the material can provide sufficient three-dimensional space for cell proliferation and crawl, so that the repaired region has good integration degree and filling degree, thereby realizing tissue repair and regeneration.

The invention belongs to the field of biomedical materials and particularly relates to a preparation method of a co-crosslinked double-network hydrogel support for promoting cartilage repair and growth. The method comprises the following steps of (1) preparation of GelMA; (2) preparation of a photo-crosslinked polypeptide; and (3) preparation of a hydrogel support, namely preparing the hydrogel support from the GelMA and the photo-crosslinked HSNGLPL under the action of a photoinitiator. The prepared hydrogel support has an obvious effect of promoting new cartilage generation and cartilage defect healing, and is capable of regulating cartilage matrix secretion of normal chondrocytes around the injured tissue, mesenchymal stem cell migration to the injured part and cartilage differentiation; by virtue of a porous structure of the hydrogel support, the HSNGLPL can be crosslinked to the surface of a porous support, endogenous TFG-beta is effectively adsorbed, the concentration of local TFG-beta beta1 is improved and the effects of the biological support in the orthopedics fields, such as a cartilage defect and a cartilage injury are achieved.

The present invention relates to compounds of Formula (I),methods for preparing these compounds, compositions, intermediates and derivatives thereof and for treating a condition including but not limited to ankylosing spondylitis, artherosclerosis, arthritis (such as rheumatoid arthritis, infectious arthritis, childhood arthritis, psoriatic arthritis, reactive arthritis), bone-related diseases (including those related to bone formation), breast cancer (including those unresponsive to anti-estrogen therapy), cardiovascular disorders, cartilage-related disease (such as cartilage injury / loss, cartilage degeneration, and those related to cartilage formation), chondrodysplasia, chondrosarcoma, chronic back injury, chronic bronchitis, chronic inflammatory airway disease, chronic obstructive pulmonary disease, diabetes, disorders of energy homeostasis, gout, pseudogout, lipid disorders, metabolic syndrome, multiple myeloma, obesity, osteoarthritis, osteogenesis imperfecta, osteolytic bone metastasis, osteomalacia, osteoporosis, Paget's disease, periodontal disease, polymyalgia rheumatica, Reiter's syndrome, repetitive stress injury, hyperglycemia, elevated blood glucose level, and insulin resistance.

The invention relates to an exosome derived from human pluripotent stem cells, a preparation based on the exosome and use of the exosome and preparation, and belongs to the technical fields of cell biology, molecular biology and drug research and development. The human pluripotent stem cell exosome or the preparation based on the human pluripotent stem cell exosome has the uses of A, being an additive cultured in vitro for maintaining the stemness of adult stem cells and inhibiting the senility of the adult stem cells; B, being an additive cultured in vitro for maintaining the proliferative activity of various tissue primary culture cells; C, being applied in preparing medicine for treating bone and cartilage degenerative changes; D, being applied in preparing medicine for preventing the bone and cartilage degenerative changes; E, being applied in preparing medicine for treating orthopedic diseases such as osteonecrosis, bone nonunion, fractures, cartilage injuries, tendon injuries, frozen shoulders or tendon adhesion. Compared with the prior art, the exosome secreted by the pluripotent stem cells has powerful functions of promoting tissue cell regeneration, preventing senility andtreating bone diseases.

Methods and devices for the regulation of matrix metalloproteinase gene expression in cartilage cells via the application of fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articular cartilage. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased cartilage tissue that include generating specific and selective electric and electromagnetic signals that generate fields optimized for reduction of matrix metalloproteinase gene expression and exposing cartilage tissue to the fields generated by specific and selective signals so as to regulate matrix metalloproteinase gene expression in such cartilage tissue. The resulting methods and devices are useful for the targeted treatment of osteoarthritis, rheumatoid arthritis, cartilage injury, cartilage defects, and tumor metastasis.

The present invention provides a composition for regenerating cartilage or treating a cartilage disease containing a monovalent metal salt of alginic acid for which the endotoxin level thereof has been lowered to an extent that does not substantially induce inflammation or fever. As a result, it is possible to provide a composition for regenerating cartilage that improves cartilage regenerative action and ease of application to a cartilage injury lesion, and a composition for treating a cartilage disease, which has the effects of protecting cartilage from mechanical irritation, inhibiting degenerative changes in cartilage caused by wear and inflammation, repairing a cartilage injury lesion, and inhibiting inflammation and pain of joint tissue.

A method for treating mammalian articular cartilage disorders, more particularly osteoarthritis, and trauma-related cartilage injuries using insulin-like growth factor I (IGF-I) is provided. The method comprises increasing the amount of IGF-I at the diseased or injured articular site to a therapeutically effective level that is capable of maintenance and / or regeneration of cartilage, which is beneficial to the long-term treatment of osteoarthritis and trauma-related injuries to cartilage tissues. In one embodiment of the invention, single doses of at least 0.01 mg of pharmaceutically effective IGF-I are administered intermittently such that the duration of time off of therapy is greater than the time on therapy, more preferably with a frequency of administration of about once per week or less.

The present invention provides an intra-articular injection administration preparation containing trehalose, which is characterized in that the preparation contains the trehalose. The present invention can improve the capabilities of the cartilage cells to adapt to and resistant to the environmental changes through the intra-articular injection administration, at the same time, the trehalose has the significant effects of antioxidant and anti-free radicals, which can alleviate the symptoms of arthritis; in addition, the trehalose also provides the necessary sugar for cartilage repair, so as to repair and reconstruct the cartilage. The present invention is used for improving and treating the rheumatism, rheumatoid arthritis, osteoarthritis, synovitis and / or cartilage injury.

The invention discloses a method for preparing a double-layer bionic cartilage tissue engineering scaffold, which comprises the following steps of: firstly, preparing a substrate; secondly, preparing the double-layer bionic cartilage tissue engineering scaffold on the surface of the substrate; and finally, soaking the double-layer bionic cartilage scaffold on the surface of the substrate by usingsolution of NaOH, and peeling the double-layer bionic cartilage scaffold from the substrate. The method has simple operation and high stability; the prepared double-layer bionic cartilage scaffold has a thin thickness and good mechanical properties and is favorable for clinical application; the scaffold is molded integrally; a vertical hole is tightly connected with a round hole to avoid the problem of breaking away from each other; the structure of the scaffold is similar to a natural cartilage structure and is hopeful to better maintain the phenotype of cartilage cells and improve the repairing effect on cartilage injuries; and the cartilage scaffold can be directly combined with a substrate comprising polylactic acid (PLA) / bone powder to form a bone cartilage scaffold used for repairing bone cartilage simultaneous colobomata, and a cartilage layer part still has a double-layer structure.

Methods and devices for the regulation of aggrecan gene expression in cartilage cells via the application of fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articular cartilage. By gene expression is meant the up regulation or down regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased cartilage tissue that include generating specific and selective electric and electromagnetic signals that generate fields optimized for aggrecan gene expression and exposing cartilage tissue to the fields generated by specific and selective signals so as to regulate aggrecan gene expression in such cartilage tissue. The resulting methods and devices are useful for the targeted treatment of osteoarthritis, rheumatoid arthritis, cartilage injury, and cartilage defects.

The invention belongs to the field of biomedicine, in particular to a bionic II type collagen hyaloplasm acid compound sponge bracket for repairing cartilage in tissue engineering. The preparation method of the II type collagen hyaloplasm acid compound sponge bracket comprises the following steps of: extracting high-purity II type collagen solution; concentrating the II type collagen solution by means of a polyethylene glycol concentration method; gradually mixing hyaloplasm acid solution with the high-purity II type collagen solution by means of a partial homogenate method; and crosslinking the concentrated II type collagen hyaloplasm acid compound sponge with EDC and NHS dual cross-linking agent to obtain the II type collagen hyaloplasm acid compound sponge bracket. The II type collagenhyaloplasm acid compound sponge bracket can be taken as a tissue engineering implant, a cell culturing bracket material or medicine controlled release and the like for the prevention and the cure of the repair of the cartilage injury.

The invention provides a xanthan-gum-containing pharmaceutical preparation for joint intracavity injection, which is characterized by containing xanthan gum. In the preparation, xanthan gum used as a pharmaceutical component for joint cavity injection has the advantages of favorable viscoelasticity and favorable lubricating property, has particular thixotropy, can be used as an excellent pharmaceutical release-control carrier, and can act in the joint cavity for a long time. The pharmaceutical preparation can improve and treat rheumatism, rheumatoid arthritis, osteoarthritis, synovitis and / or cartilage injury, and effectively relieve pain of patients.

The present invention relates to methods of using zFGF5 compositions to proliferate chondrocytes and their progenitors, and to induce deposition of cartilage. zFGF5 compositions are disclosed for treating disorders associated with chondrocytes, such as cartilage injuries and defects. In addition, methods for treating neurological disorders, such as stroke, are disclosed, and methods for using zFGF5 compositions to stimulate growth of cells associated with neurological injury and disease are disclosed.

A method for treating mammalian articular cartilage disorders, more particularly osteoarthritis, and trauma-related cartilage injuries using insulin-like growth factor I (IGF-I) is provided. The method comprises increasing the amount of IGF-I at the diseased or injured articular site to a therapeutically effective level that is capable of maintenance and / or regeneration of cartilage, which is beneficial to the long-term treatment of osteoarthritis and trauma-related injuries to cartilage tissues. In one embodiment of the invention, single doses of at least 0.01 mg of pharmaceutically effective IGF-I are administered intermittently such that the duration of time off of therapy is greater than the time on therapy, more preferably with a frequency of administration of about once per week or less.

A method for preparing PRP (platelet-rich plasma) of umbilical cord blood includes steps: subjecting fresh umbilical cord blood to low-speed centrifuging, removing a lower cell layer, and subjecting upper platelet containing plasma to ultrahigh-speed centrifuging until the liquid is separated into two layers including an upper plasma layer and a lower layer of precipitates which are platelets; removing the upper plasma layer, leaving a small quantity of plasma in an original centrifugal tube, well mixing, vibrating, counting the platelets, and well mixing according to a proportion that 1ml of plasma contains 1.2+ / -0.125 billion of platelets approximately. The platelet-rich plasma is effective in treatment of cartilage injuries, pain alleviation and function restoration and especially remarkable in treatment of senile osteoarthritis.

The invention is a method for the treatment of mammalian articular cartilage disorders, inflammatory joint disease, trauma-related cartilage injuries, and degenerative disc disease. The method involves treating the affected area with a composition containing a therapeutically effective dose of Centella extract. The composition is delivered locally by parenteral administration to the affected site.

The present invention provides a method for treating at least one of the following: osteoarthritis, rheumatoid arthritis, cartilage injury, and a cartilage defect containing the following steps. First, a defective cartilage tissue is provided. Second, a solution containing ions for treating defective cartilage tissue is provided. Third, the solution containing ions is introduced about the defective cartilage tissue. Fourth, an electrical source generates electricity. Fifth, the solution containing ions and an area about the defective cartilage tissue is contacted with the electricity from the electrical source. Sixth, the electrical source is powered on to provide electricity, whereby the ions move from the solution and into the defective cartilage tissue.

The invention discloses a cartilage graft for cartilage injury repair and a preparation method thereof. The cartilage graft is prepared based on an adipose-derived stem cell-cartilage cell co-culture technology. The preparation method is effective and safe, can prepare enough cartilage cells for transplant in a short time, can efficiently transform cartilage tissue and can improve cartilage tissue transplant treatment effects. The preparation method utilizes nutrition functions of adipose-derived stem cells and carries out co-culture of adipose-derived stem cells and cartilage cells in a three-dimensional culture system. Through interaction of the adipose-derived stem cells and the cartilage cells, proliferation and activity of the cartilage cells are promoted. The adipose-derived stem cells are influenced by the environment and are partly differentiated into cartilage cells and thus under the condition of undersampling or activity lack of the cartilage cells, a high-quality cartilage graft can be obtained and graft viability is promoted and shallow-layer cartilage coloboma can be repaired well.

The adipose-derived stem cells are derived to express bone morphogenetic protein (BMP-4) or transforming growth factor (TCF-beta3) through a gene transduction technology. The performance-enhanced stem cell has high multiplication capacity and cartilage repair capacity and is a good tool for repairing cartilage injury. Considering the functions of the BMP-4 and TCF-beta3 on stem cell regulation and nutrition, the trophic factors are fully expressed by the stem cells through a virus vector transduction method, the capacity of culturing the improved adipose-derived stem cells and cartilage cells in a cartilage induction medium for differentiating into cartilage cells is higher than that of the common adipose-derived stem cells, more type II collagens are generated, and the formed cartilage sphere is large and dense; and meanwhile, the survival cycle of the transplanted stem cells in vivo is prolonged, the repair function of the growth factors and trophic factors on the damaged part is prolonged, and cartilage growth and wound healing are promoted.

Disclosed is a method for preparing cross-linked hyaluronic acid-based cell scaffold material. The hyaluronic acid-based cell scaffold is obtained by subjecting a hyaluronic acid and a disulfide cross-linking agent to an amidation reaction, followed by dialysis-freeze drying. The cell scaffold has abundant pores, good mechanical strength which ensures that the scaffold does not rupture in transplantation, and good biocompatibility. The method is advantageous in that the raw material is easy to obtain, the reaction condition is moderate, and the process is simple. Cross-linked networks of the prepared hydrogel contain disulfide bonds, which can quickly split into single chains at the presence of small molecular glutathione. The hyaluronic acid-based cell scaffold has flexibly controllable mechanical property, disaggregation ability, and swelling property, and therefore has wide applications in facilitating cartilage injury repair, skin repair, cell culture, etc.

The invention discloses a mesenchymal stem cell preparation for repairing articular cartilage injury or coloboma, a preparation method and application thereof. According to the preparation method, mesenchymal stem cells and synovial cells are indirectly cocultured, so as to obtain the mesenchymal stem cells after coculture, and then the mesenchymal stem cells are cryopreserved, umbilical cord mesenchymal stem cells, which are acquired after the synovial cells and the umbilical cord mesenchymal stem cells are inoculated at density ratio of 4:1 and cocultured, are easier to differentiate towardcartilage direction under a same induction condition. The preparation method utilizes a constructed animal knee joint cartilage injury or coloboma model to prove that the umbilical cord mesenchymal stem cells cocultured with the synovial cells have an obvious effect of facilitating the repairing of articular cartilage injury or coloboma. The invention further provides a preparation which is prepared from the cocultured mesenchymal stem cells. The preparation can be applied to clinic repairing of articular cartilage injury or coloboma; the preparation can effectively promote the repairing of articular cartilage injury or coloboma through anticular injection; the therapeutic effect of the preparation is obviously better than that of a pure mesenchymal stem cell preparation.

The invention discloses an inhibitor for lncRNA-MSR and application of the inhibitor, and belongs to the field of bioengineering. The inhibitor can inhibit expression of the lncRNA-MSR to decrease the level of an expression product of the lncRNA-MSR, and a nucleotide sequence of the lncRNA-MSR is shown as SEQ ID NO:1. By providing the inhibitor capable of inhibiting expression of the lncRNA-MSR, degradation, caused by expression of the lncRNA-MSR, of an extracellular matrix in cartilage tissue can be blocked, and an important significance on prevention and treatment of osteoarthritis cartilage injuries is achieved.

The present invention provides a composition for regenerating cartilage or treating a cartilage disease containing a monovalent metal salt of alginic acid for which the endotoxin level thereof has been lowered to an extent that does not substantially induce inflammation or fever. As a result, it is possible to provide a composition for regenerating cartilage that improves cartilage regenerative action and ease of application to a cartilage injury lesion, and a composition for treating a cartilage disease, which has the effects of protecting cartilage from mechanical irritation, inhibiting degenerative changes in cartilage caused by wear and inflammation, repairing a cartilage injury lesion, and inhibiting inflammation and pain of joint tissue.