Mesenchymal stem cell preparation for repairing articular cartilage injury or coloboma, preparation method and application thereof
A technology for stem cell and cartilage damage, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve the problems of weakened cell differentiation function state, cell concentration and quality affecting repair effect, etc., to achieve the effect of promoting repair
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Embodiment 1
[0030] Preliminary Example 1 Isolation and Culture of Synoviocytes Used in Co-Culture
[0031] 1) Screen patients without immune diseases such as rheumatism, rheumatoid and osteoarthritis, and collect healthy synovial tissue from the knee joint of patients with meniscus or cruciate ligament injury or synovial donors through arthroscopic surgery, and transfer the tissue to Stored in PBS buffer containing 1% double antibody.
[0032] 2) Under aseptic conditions, the synovium tissue was placed in a petri dish and shredded, washed 3 times with PBS buffer solution, and collected into a centrifuge tube.
[0033] 3) Add 4% type I collagenase 5 times the volume of the tissue, place it in a constant temperature shaker, and digest at 150 rpm at 37°C for 2-3 hours.
[0034] 4) After the tissue block was completely digested, the cell suspension was filtered through a 70 μm cell strainer, and the collected filtrate was centrifuged at 1500 rpm for 5 min.
[0035] 5) Discard the supernatan...
Embodiment 2
[0057] The preparation of embodiment 2 preparations
[0058] The 11 groups of co-cultured umbilical cord mesenchymal stem cells cryopreserved in Example 1 were treated as follows: resuscitated in a 37°C water bath, washed once with normal saline, centrifuged at 2000rpm for 5min, and the supernatant was discarded.
[0059] 1) Resuspend the cells with normal saline as an adjuvant, and adjust the cell concentration to 1×10 6 / mL, 1mL each, inhaled into a syringe for use.
[0060] 2) Resuspend the cells with physiological saline as an adjuvant, and adjust the cell concentration to 10×10 6 / mL, 1mL each, inhaled into a syringe for use.
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