134results about How to "Promotes damage repair" patented technology

The invention discloses a polysaccharide-dopamine composite biogel and application of the biogel. A preparation method of the biogel comprises the following steps: dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide sodium in an MES (Methyl Ester Sulfonate) buffer solution with a pH value being 5-6 in an inert gas atmosphere so as to prepare an EDC (ethylcarbodiimide) mixed solution, subsequently adding the EDC mixed solution into a polysaccharide solution for reacting at 20-30 DEG C for 0.5-1 hour, then adding a dopamine solution for further reacting at 20-30 DEG C for 1-4 hours, dialyzing a reactant by taking distilled water as a dialyzate, taking trapped fluid for freezing and drying to obtain the polysaccharide-dopamine composite biogel. The polysaccharide-dopamine composite biogel is simple and effective in modification method and capable of increasing the surface viscidity of the cartilage tissue, reducing loss of therapeutic drugs or implanted cells and facilitating the repairing of the cartilage injury.

The invention provides a a design strategy of a novel adhesive hydrogel material and obtains a high-extension, self-healing, and high-adhesion biocompatible hydrogel material. Based on a double network structure design principle, polymerizable polyethylene glycol-based high polymer constitutes a first covalent crosslinked network, and hydrophilic high polymer with ultra-high molecular weight forms a second noncovalent crosslinked network through entanglement. A mesh size of a first network is larger than a molecular effective size of second network high polymer, and molecular weigh of high polymer forming a second network should be larger than a critical entanglement molecular weight thereof, so that effective molecular entanglement is formed, high-extension, self-healing, and high-adhesion characteristics are given to double network hydrogel. The novel hydrogel can rapidly and repeatedly form stable adhesion with surfaces of various materials. Damaged by an external force, the hydrogel can rapidly and repeatedly perform self healing. The material can be used as a cell cultivating carrier, a sewing-free novel dressing, and can be applied to wearble sensors.

The invention discloses a method for preparing ultra-low molecular weight hyaluronic acid oligosaccharides and a salt thereof through combination of solid-liquid biphasic enzymolysis and ultrafiltration. The method comprises the following steps: degrading hyaluronic acid and a salt thereof by utilizing hyaluronidase produced by bacilli; preparing high-concentration hyaluronic acid enzymatic hydrolysate by adopting the method of combining a solid-liquid biphasic enzymolysis system and an ultrafiltration system, and then performing inactivating, activated carbon adsorption and impurity removal,filtering and spray-drying to obtain the ultra-low molecular weight hyaluronic acid oligosaccharides and the salt thereof with the molecular weight of less than or equal to 3 kDa. The product preparedby the method is high in stability, ultra-low in molecular weight, good in inflammation resistance and percutaneous absorptivity, high in purity, strong in oxidation resistance, does not have cytotoxicity, has the advantages of repairing skin cell damage and the like, and can be widely applied to the fields of cosmetics, food and medicines. According to the method, the process flow is easy to operate; conditions are mild; various alcohols are not used; the energy consumption is relatively low; the method does not have damage to a product structure, does not have environmental pollution, and is suitable for large-scale industrial production.

The invention discloses self-healing hydrogel capable of promoting wound healing and cancer therapy and preparation method thereof. The self-healing hydrogel is FCB hydrogel which is prepared by Schiff base reaction among a polyether-cationic polymer water solution with a mass concentration as 8% to 50%, a polyether-p-hydroxy benzaldehyde water solution with a mass concentration as 10% to 40% anda dopamine modified biological active inorganic material water solution with a mass concentration as 2% to 10%, the water solutions are mixed according to a volume ratio of (22 to 28) to (7 to 10) to(4 to 10), the F is polyether-cationic polymer, the C is polyether-p-hydroxy benzaldehyde, and the B is a dopamine modified biological active inorganic material. The self-healing hydrogel has good biological compatibility, better photo-thermal character and stronger antibacterial property; under laser radiation, the self-healing hydrogel can effectively inhibit animal cancer growth; furthermore, the self-healing hydrogel can promote mouse skin damage repair. The preparation method has the advantages of simpleness, no organic solvent residue, environment-friendly synthesizing method, convenience in operation and low raw material cost.

The invention discloses hydrogel for repairing injured tissue and a preparation method thereof. The preparation method comprises the following steps: dissolving a hydrogel material through sterile distilled water, and swelling to obtain hydrogel; dissolving PGE2 powder into a phosphate buffering solution to prepare a PGE2 solution; dropwise adding the PGE2 solution into the hydrogel; stirring for 3-5 hours under the temperature of 2-8 DEG C; storing or storing by freezing, wherein the PGE2 is the abbreviation of prostaglandin E2. With the adoption of the hydrogel, the release time of PGE2 can be certainly prolonged; the hydrogel is directly applicable to the treatment of tissue injury, and is capable of promoting the recovery of injured skin tissue and function; the hydrogel is capable of inducing macrophage at the injured site to polarize to reach M2 type so as to inhibit inflammatory, thereby accelerating the healing of the injured tissue; in addition, the blood vessel at the injured part is promoted to regrow in early stage. Additionally, the hydrogel is free from cytotoxicity, can directly coat the surface of the injured tissue, is convenient to use, and low in price, and has an effective treatment effect.

The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells / 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells / one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells / one time / one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.

The invention provides a skin moisturizer for treating a skin disease and a preparation method of the skin moisturizer. The skin moisturizer is mainly prepared from the following raw materials in parts by weight: 10-20 parts of alginate oligosaccharide, 0.5-5 parts of a solubilizer, 0.5-1 part of sodium chloride, 5-10 parts of a dispersing agent, 5-20 parts of a tackifier, 2-3 parts of a preservative and 2-3 parts of an antioxidant, wherein the solvent is injection water; and the preparation method comprises the following steps: dissolving 0.2-0.3 part of sodium chloride into residual injection water; adding the solubilizer, the tackifier, the preservative, the dispersing agent and the antioxidant, stirring evenly, and adjusting the pH value to be 3.5-6.5; dissolving the alginate oligosaccharide into the residual injection water, adding residual sodium chloride, stirring and mixing with the substances obtained from the steps; and packing and sterilizing to obtain the skin moisturizer. The skin moisturizer provided by the embodiment has the effects of good treatment effect, mild medicine property and no side effect.

The invention relates to the field of biomedicines and discloses an electrical stimulation system for promoting in vivo nerve repair. The electrical stimulation system comprises a nanogenerator, a wire and an electrode, wherein the nanogenerator is connected with the electrode through the wire to transmit current or voltage of the nanogenerator to the electrode; and the electrode can apply the current or voltage to an organism. The system for promoting in vivo nerve repair disclosed by the invention has the advantages that an electrical stimulation source is simple in structure, environment-friendly in materials, wide in material source, easy to be flexible and easy to miniaturize, and can be self-driven by motion and force of a body to generate electricity.

The invention discloses an application of L.p R3-10 in preparation of medicines for preventing or treating oral inflammatory diseases, and belongs to the technical field of microorganisms. Inactivatedand non-inactivated fermentation supernatant and bacterial suspension of the L.p R3-10 disclosed by the invention have the effect of inhibiting LPS from stimulating human gingival fibroblasts to secrete cell inflammatory factors IL6, TNF-alpha and PGE2 in vitro; in an in-vivo inflammation model, neutrophils and macrophages can be remarkably inhibited from being gathered to the inflammation position of the tail fin of a zebra fish, and clearing of the neutrophils and the macrophages at the inflammation position of the tail fin of the zebra fish is remarkably promoted; and meanwhile, damage repair of the tail fin of the zebra fish can be remarkably promoted. The L.p R3-10 disclosed by the invention has a huge potential application prospect in the aspect of treating and / or preventing the oral inflammatory diseases.

The invention relates to an exosome preparation for promoting skin wound healing and a preparation method thereof, and belongs to the technical field of stem cells. The exosome preparation for promoting skin wound healing is prepared from the following components in parts by mass: 30 to 50 parts of stem cell exosome extract, 10 to 20 parts of plumeria rubra extracting solution, 10 to 20 parts of witch hazel extract and 10 to 20 parts of chamomile extract. The preparation method is simple, and all the raw materials are uniformly mixed in parts by mass. By adding stem cell exosome and the components such as plumeria rubra, chamomile, witch hazel and the alginate, wound hemostasis is accelerated, and injured tissue repair and skin regeneration are promoted; and meanwhile, the effects of resisting inflammation, inhibiting bacteria, relieving itching and moisturizing are achieved. The product has the characteristics of good effect, quick effect, convenient use, no irritation to skin mucosa,safety, reliability and no scar.

The invention provides a camellia nitidissima ginseng tablet candy, which comprises the following raw materials by weight: 18-24 parts of camellia nitidissima, 4-8 parts of ginseng, 16-22 parts of kudzuvine root, 11-15 parts of moringa seed, 8-12 parts of pine pollen, 8-12 parts of Chinese wolfberry, 8-12 parts of Maca, 6-10 parts of cordyceps militaris, 6-10 parts of red dates, and 6-10 parts of honey. The invention also provides a preparation method of the camellia nitidissima ginseng tablet candy. All the raw materials of the camellia nitidissima ginseng tablet candy cooperate with each other to synergistically act on the endocrine system, thus gradually activate the female ovarian functions, regulating ovary mitochondria hormone secretion, improving the sexual life quality of husband and wife, expelling toxin and helping sleep, increasing skin elasticity, preventing and treating gynecological diseases, significantly reducing chloasma, and fully restoring the maiden youthful vitality of the body.

The invention provides application of leaf mustard polypeptide to preparation of skin whitening and repair cosmetics. The leaf mustard polypeptide has small molecular weight, can be easily absorbed by the skin and can aim at the problem of pigmentation in the skin damage repair process when being used for preparing the cosmetics; the acceleration of the skin damage repair process is promoted; the skin cell growth differentiation is accelerated; the repair time is shortened; the skin is protected, so that the hyperpigmentation of the skin caused by protection reactivity is relieved.

The invention discloses an amplification culture method for cloning adipose mesenchymal stem cells. The amplification culture method includes four steps of (1), extracting platelet-rich fibrin glue; (2), preparing special complete culture media; (3), carrying out isolated culture and subculture on primary adipose mesenchymal stem cells; (4), acquiring biological characteristics of different types of amplified adipose mesenchymal stem cells. The amplification culture method has the advantages that high-purity CD54 (+) adipose mesenchymal stem cell population can be obtained by the aid of the amplification culture method and is high in clone formation ability, self-renewal ability and adipogenic differentiation potential, novel methods can be provided for acquiring large quantities of stem seed cells in an in-vitro manner and applying the stem seed cells to regenerating adipose tissues, a novel treatment strategy can be provided for improving clinical repair for injury of soft tissue or congenital defects and abnormality of soft tissues of organs on body surfaces, and sufficient theoretical bases can be provided for guaranteeing the safety of clinical application of the adipose mesenchymal stem cells in the future.

The invention discloses a product for regulating blood pressure. The product for regulating blood pressure is prepared from the following raw materials: 5-6% of honeysuckle flower extract, 3-4% of a ginseng extract, 3-4% of oyster peptides, 7-8% of a kudzuvine root extract, 7-8% of a Chinese wolfberry fruit extract, 1-2% of a cordyceps militaris extract, 14-21% of a hawthorn fruit extract, 3-5% ofa stigmata maydis extract, 1-3% of a winter gourd extract, 1-4% of a fructus gardeniae extract, 4-5% of a mint extract, 2-5% of rhizoma polygonati, 3-5% of seabuckthorn fruits, 17-18% of maltodextrin, 1% of corn starch, and 1% of magnesium stearate. As for the product for regulating blood pressure prepared according to the invention, the various components are proportioned and prepared by adopting an appropriate ratio so that the product has good effects on lowering blood pressure and blood lipid; and moreover, the product is capable of rapidly passing through the stomach to the small intestine so that the product can be quickly and completely absorbed so as to quickly supplement nutrients needed by patients with hypertension. Being frequently taken, the product for regulating blood pressure is capable of conveniently and quickly enhancing body immunity.

The invention discloses a bionic nano fiber material as well as a preparation method thereof and application thereof, and relates to the technical field of medical biological materials. The bionic nano fiber material comprises a silk fibroin-chitosan nano fiber film and liquid crystal state collagen, wherein one part of liquid crystal state collagen is filled into holes of the nano fiber film, andthe other part of the liquid crystal state collagen is coated to the surface of the nano fiber film. The preparation method comprises the following steps: compounding the silk fibroin-chitosan nano fiber film with a high-concentration collagen solution to obtain a mixture; balancing the mixture in acetic acid solution steam, and self-assembling the collagen into the liquid crystal state collagen.The liquid crystal state collagen can be used as cell activating mesenchyme of the silk fibroin-chitosan nano fiber film, can adhere a great number of cells, can trend a cell growth direction, and also can form long-bundle fibers, so that mechanical properties of the nano fiber film are improved. The bionic nano fiber material has good mechanical property, relatively strong cell adhesion and no immunogenicity, is beneficial for cell repair, and can be applied to dressing, scaffolds for tissue engineering and drug delivery bodies.

The invention provides a composite material capable of regulating and controlling Reactive Oxygen Species (ROS) and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The composite material capable of regulating and controlling the reactive oxygen species comprises an ROS scavenging matrix and a matrix carrier, wherein the ROS scavenging matrix is at least one of hMnO2 and mPDA; and the matrix carrier is a biocompatible material. The composite material prepared by the method can regulate and control the ROS, and can further promote tissue repair by releasing a loaded medicament and / or a growth factor after the medicament and / or the growth factor are / is loaded.

The invention discloses an application of an NK cell stock solution in preparation of a medicine for treating skin diseases. An NK cell is an important immune cell for an organism, and not only is related to tumor resistance, viral infection resistance and immune regulation, but also takes part in happening of hypersensitive responses and autoimmune diseases in some cases. The NK cell stock solution contains main components of NK cell excreted proteins and cell factors, including MIP-1alpha, MIP-1beta, RANTES, monocyte chemoattractant proteins, interleukin, colony stimulating factors, tumor necrosis factors, interferon and perforin, and these proteins and cell factors can be absorbed by the skin and directly act on target cells to kill or inhibit pathogenic microorganisms including bacteria, fungi, viruses and the like, improve an immune system of the skin, repair the damaged skin, and achieve an aim of comprehensively treating various skin diseases; and after the use of the NK cell stock solution, a protective film is formed on the surface of the skin, which is beneficial for rapid repair of the damaged skin and prevention of repeated aggravation of an illness.

The invention discloses a traditional Chinese medicinal composition for treating chronic atrophic gastritis and a preparation method thereof. The active ingredients of the traditional Chinese medicinal composition for treating the chronic atrophic gastritis are prepared from the following raw materials in parts by weight: 10-30 parts of astragalus, 5-20 parts of radix pseudostellariae, 5-20 parts of white atractylodes rhizome, 5-20 parts of poria cocos, 5-20 parts of curcuma zedoary, 2-10 parts of gecko, 10-25 parts of hedyotis diffusa, 10-25 parts of hericium erinaceus and 3-10 parts of panax notoginseng powder. Commonly used auxiliary materials can be added into the traditional Chinese medicinal composition, and a common traditional Chinese medicinal preparation is prepared. The preparation method of the traditional Chinese medicinal composition comprises the following steps: smashing gecko and panax notoginseng powder into fine powder; extracting volatile oil from curcuma zedoary, extracting herb residue and residual raw materials with water, filtering, mixing extracting solution, and concentrating to obtain extract; drying the extract into dry extract, smashing, mixing with the fine powder, pelletizing, and adding the volatile oil, so that the traditional Chinese medicinal composition is obtained. Experiments prove that the traditional Chinese medicinal composition has the effect of preventing and treating the chronic atrophic gastritis and gastric precancerous lesions.

The invention relates to aromatic therapy wax for massage with a skin protection function, which is prepared from the following materials in parts by weight: 75-92 parts of partially hydrogenated triglyceride, 5-10 parts of sea buckthorn fruit oil, 1-3 parts of geranium essential oil, 0.5-10 parts of azone, 0.1-0.5 parts of ethyl hydroxybenzoate, and 0.5-2 parts of tourmaline powder with granularity between 1250 and 3000. The aromatic therapy wax for the massage is illuminated by infrared rays and molten continuously, and emits aroma of the geranium essential oil, so as to recover mental balance, relieve pressure, and revivify spirit. A tepid wax solution of the aromatic therapy wax is applied on the skin and the massage is conducted continuously, with the combined action of the sea buckthorn fruit oil and the geranium essential oil, the nutrition absorption velocity of the skin is increased, an injured skin tissue is repaired, the immunological competence of the skin tissue is enhanced, the free radical is eliminated, the oxidation is resisted, the elasticity is improved and the senescence is postponed.

The invention relates to a repair skin care product, in particular to a raw material of the skin care product which contains the compound growth factor for repair and care of the skin and anti-aging and the repair skin care product which uses the raw material. The invention provides the compound growth factor skin care product raw material and the repair skin care product which uses the raw material, so as to overcome the problems that the prior art is difficult to maintain the biological activity and bioavailability and does not have the function of comprehensive conditioning and repair simultaneously, and the effect is not ideal. The invention adopts the following technical proposals: the compound growth factor skin care product raw material is characterized in that the raw material is prepared by 1 to 1.2 parts by weight of HGH and 0.5 to 1 part by weight of IGF-1 through the nanoliposome encapsulation technology. The repair skin care product which uses the raw material includes freeze-dried powder, skin cream, skin lotion, body wash, eye cream, skincare water, facial cleanser, nutrition mask, massage cream, anti-wrinkle cream, repair essence, hand cream, and other various forms.

The invention provides a dietary fiber product which comprises water-soluble dietary fiber, water-insoluble dietary fiber, yeast glucan, oat glucan and soybean oligosaccharide, wherein the water-soluble dietary fiber, water-insoluble dietary fiber, yeast glucan, oat glucan and soybean oligosaccharide are proportioned according to the principle of monarch, minister, assistant and guide; and the soybean oligosaccharide is a monarch drug. The invention also provides a preparation method of the dietary fiber product. The dietary fiber product provided by the invention can efficiently and effectively promote the growth of intestinal probiotics (bifidobacteria) so as to realize the effects of promoting damage repair of intestinal mucosa, promoting elimination of heavy metals, improving constipation and reducing cholesterol. After one month of eating, the constipation is improved by 98%, the defecate forming rate reaches 95%, the halitosis is improved by 96%, the postprandial blood sugar is lowered by 100%, and the plasma cholesterol is reduced by 100%.

The invention discloses a functionalized nerve regeneration collagen scaffold, a preparation method and an application thereof. The functionalized nerve regeneration collagen scaffold comprises: a linear, ordered collagen fiber scaffold for guiding nerve regeneration, and a CBD-SDF having collagen binding ability and combined with the collagen fiber scaffold. The functionalized nerve regenerationcollagen scaffold provided by the invention can slowly release CBD-SDF to improve the efficiency of recruiting endogenous neural stem cells, also provides a good microenvironment for cell adhesion, axon growth and elongation, and also inhibits glial scar formation. In addition, the functionalized nerve regeneration collagen scaffold with VPA are combined to promote the homing of endogenous neuralstem cells to the injury site after spinal cord injury and regulate the differentiation into neurons, promote nerve regeneration and synapse formation, inhibits of glial scar formation, and ultimatelypromotes spinal cord injury repair and motor function improvement.

The invention provides an assembly type building beam column joint structure and a construction method thereof, and relates to a beam column joint and a construction method. Two to four steel beams are connected between an upper steel pipe column and a lower steel pipe column; flat plates and flange plates are arranged at the two ends of joint connecting seats respectively; the two joint connecting seats are fixed to the lower end of the upper steel pipe column and the upper end of the lower steel pipe column respectively; two spherical positioning grooves are concavely formed in each side of the periphery of the surface of each flat plate; two ball head lap joint rods are arranged at the connecting ends of the steel beams and clamped between the spherical positioning grooves corresponding to the two flat plates; the edges of the two flat plates are connected and fixed through fastening bolts and nuts; the upper portions of the steel beams are connected with the upper steel pipe column in a suspended mode through steel wire ropes; and energy dissipation steel plates are obliquely supported between the lower portions of the steel beams and the lower steel pipe column. A connecting structure similar to a spherical hinge is adopted between the beam columns, the upper portion is suspended through the steel wire ropes, the energy dissipation steel plates are obliquely supported and installed on the lower portion, the efficient energy dissipation effect is achieved through mutual cooperation, the structure is stable, and assembly and damage repair are facilitated.

The invention belongs to the field of biomedicine and particularly relates to an application of a recombinant AAV (adeno-associated virus) carrying a Lin28a gene in preparation of a wound healing promoting drug. The Lin28a gene is inserted to an AAV vector, and the recombinant AAV carrying the Lin28a gene is acquired. A preparation method of the recombinant AAV is simple, skin wound healing can be effectively promoted, regeneration of part of deeply burnt dermis can be remarkably promoted, bone injury and soft tissue injury healing can be promoted, and further, scar formation can be inhibited.

The invention discloses a device and method for measuring a laser damage three-dimensional structure, and belongs to the field of phase shift digital holography. According to the invention, a corresponding phase shift amount is introduced according to a phase shift algorithm, and a corresponding hologram is recorded by CCD imaging equipment. Compared with other methods, the method is simple in structure and easy to operate, the cost of laser damage detection is reduced, rapid and nondestructive measurement of three-dimensional damage is achieved, and convenience is brought to damage repair.

The invention discloses an active extracellular matrix, a composition containing the active extracellular matrix, a 3D tissue repair scaffold, and a preparation method and application. The method comprises the following steps of introducing a gene segment for expressing an active factor into a cell in advance, and carrying out in-vitro cell culture to obtain the extracellular matrix containing theactive factor, so that the reliability of cell origin is ensured while imparting stability and modifiability or processability to the quality of extracellular matrix products. According to the method, stem cell culture and directional differentiation are carried out on a 3D printed porous scaffold to prepare a bionic scaffold capable of promoting damage repair, a material design principle and a stem cell differentiation technology based on stem cell combined directional differentiation are established, and a new field of stem cell and material combination in in-situ tissue engineering repairapplication is developed. According to the method, the bionic scaffold is further optimized, induced adsorption, growth and differentiation of cells after implantation are achieved, and tissue (bone,cartilage, skin, myocardium and blood vessels) regeneration is achieved.

The invention provides a novel application of betulin derivatives shown in structural formulas I, II, III, IV, V, VI, VII, VIII, IX and X or pharmaceutically acceptable salts and pharmaceutical compositions of the betulin derivatives in preparation of medicines for repairing nerve injury. The compound can be further used for preparing medicines for treating neurodegenerative diseases, traumatic brain injury and stroke. According to the invention, the medicinal field of the betulin derivative modified by the mother nucleus structure is expanded, and a new choice is provided for developing neuroprotective drugs.

The invention relates to a traditional Chinese medicine composition for repairing ephedrine tissue damage. The traditional Chinese medicine composition comprises the following components in percentage by weight: 40-50% of astragalus, 35-45% of Sichuan lovage rhizome and 10-20% of liquorice. The preparation method of aqueous solution of the traditional Chinese medicine composition comprises the following steps of: (1) respectively taking out equal astragalus, Sichuan lovage rhizome and liquorice, respectively adding 5 times of water, soaking for 1h, decocting for 30min, filtering medicine liquid for standby, respectively adding 5 times of water to the decoction dreg, decocting for 30 minutes, and filtering medicine liquid for standby; (2) taking medicine liquid of the step (1) twice, respectively mixing and concentrating constant volume of the medicine liquid to be 250mL to prepare concentrated liquids; and (3) taking out each concentrated liquid in the step (2), and mixing according to formula proportion. According to the traditional Chinese medicine composition for repairing the ephedrine tissue damage, disclosed by the invention, the anti-oxidation capability of tissue cells can be improved, and peroxidation of cell lipid is suppressed; in addition, by improving the activity of cells, the cell apoptosis is suppressed, the tissue cell damage is released, the tissue cell repair is facilitated, the constitution of a patient is improved, and the certain treatment or withdrawal function is provided for ephedrine-habituated animals.

The invention relates to a compound marssonia extract as well as a preparation method and application of the compound marssonia extract. The compound marssonia extract disclosed by the invention contains a plurality of growth factors and proteins with the functions of regulating and controlling cell proliferation and differentiation, repairing cells and nourishing cells, maintaining tissue and cell growth and development and the like, promotes cell repair and organ injury repair, delays skin aging, reduces or even eliminates melanin deposition, and is used for joint repair, hair follicle repair, organ injury repair, skin cell repair, oxidative injury cell repair and the like, and is safe and effective.

The invention discloses umbilical cord mesenchymal stem cell external gel for promoting trauma healing. The umbilical cord mesenchymal stem cell external gel is prepared by the following steps of carrying out subculture on resuscitated umbilical cord mesenchymal stem cells, and carrying out an enrichment and concentration method to obtain high-concentration umbilical cord mesenchymal stem cells with biological activity; and matching with sodium alginate gel joint preparation to obtain the umbilical cord mesenchymal stem cell gel with high stability and permeability. The gel provided by the invention promotes the growth of the cells at a wound by applying the gel to the local wound in vitro, meanwhile, avoids bacterial invasion, reduces the possibility of infection, and provides a microenvironment beneficial to tissue growth for the wound, thereby promoting trauma healing, shortening the treatment time and reducing the pain of the wounded. The umbilical cord mesenchymal stem cell external gel is simple in operation, the finished product is convenient to store, the treatment cost of the wounded is reduced, and the treatment effect is improved.