69results about How to "Weak immunogenicity" patented technology

The invention provides an application of umbilical cord mesenchymal stem cells in preparation of formulation for treating lupus erythematosus, in particular to an application in preparation of formulation for treating recurrent systemic lupus erythematosus. The invention further provides a method of preparing umbilical cord mesenchymal stem cells of formulation capable of being used for treating the lupus erythematosus and a formulation composition having the stem cells. The umbilical cord mesenchymal stem cells prepared by the invention have the advantages of low immunoreaction risk, high activity, low residue, and the like, and the preparation cost is low.

The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells/ 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells/ one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells/ one time/ one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.

The invention belongs to the field of molecular biology, and particularly relates to an EpCAM single domain antibody G7 and a nucleotide sequence for encoding the EpCAM single domain antibody G7. By utilizing a phage display technology, a single domain heavy chain antibody library specifically bound with a target antigen CD326 is screened from a camel-source immunizing single domain heavy chain antibody library, and a coding sequence of a positive clone is further guided into a prokaryotic expression vector, so that the high-efficiently expressed EpCAM single domain antibody G7 is obtained. Therefore, a basis is provided for clinic targeted immunological therapy of CD326.

The invention aims to provide polypeptide with immunomodulatory effects, namely undecapeptide which has immunomodulatory effects and is separated from bursa of Fabricius. The amino acid sequence of the polypeptide is SEQ ID NO:1. The bursa of Fabricius undecapeptide separated from a bursa of Fabricius extract product has a simple structure, has no toxic or side effect, and has poor immunogenicity. The polypeptide can be obtained by separation and extraction from bursa of Fabricius and also can be obtained by chemical synthesis. The polypeptide has low cost and can be put into mass production. The polypeptide has an induction effect on antibody production, and also can regulate production of cytokines and proliferation of lymphocyte and promote cellular immune response. In addition, the polypeptide also regulates differentiation of B cell in a dose-dependent mode. The polypeptide has a wide application prospect, taking an application of the polypeptide in the aspects of immunomodulation, treatment and the like for example.

The invention discloses a composition based on stem cells and application thereof in preparing preparation for the coronary heart disease. The composition based on the stem cells includes the stem cells prepared through an optimizing method and cell preserving liquid serving as a solvent. Firstly, proper stem cells are selected; secondly, the selected stem cells are further processed through the optimizing method; lastly, the stem cells are collected and resuspended in the cell preserving liquid. A various cell implanting method is adopted in the application of the composition based on the stem cells in preparing the preparation for the coronary heart disease, the time nodes before an operation, during the operation and after the operation are combined, and it is ensured that the transplanted stem cells can give the full play. The optimizing method is used for preparing the stem cells and the stem cell composition in the cell preserving liquid, the ventricle function can be extremely obviously enhanced after cell transplantation, and the left ventricle reconstitution is restrained. The optimal cell composition is provided for treating the coronary heart disease, the source is wide, preparation is simple, toxic and side effects are avoided, and the transplanting effect is good.

A combined application of Haemophilus parasuis (HPS) LC strain having a deposit number of CGMCC No. 5257 and HPS LZ-20100109 strain having a deposit number of CGMCC No. 5802 in preparing a bivalent inactivated vaccine is provided, relating to HPS disease vaccines in a field of veterinary biologics. The combined application of the HPS LC strain and the HPS LZ-20100109 strain in preparing the bivalent inactivated vaccine is safe and reliable, providing not only a homologous challenge protection against serotype 1 and serotype 5, but also certain cross protection against heterologous challenges of serotype 2, serotype 4, serotype 10, serotype 12, serotype 13, serotype 14, and serotype 15 of HPS. The combined application of the HPS LC strain and the HPS LZ-20100109 strain has an obviously increased effect. After immunizing swine, a relatively strong immunity is generated; an incidence rate and a mortality rate of inoculated swine decrease obviously.

The invention discloses an active polypeptide with an antagonistic chemotactic factor receptor CCR5, which has an amino acid sequence in SEQ ID NO:1 or an amino acid sequence obtained by carrying out deletion, addition, insertion and substitution of one or more amino acid residues on the amino acid sequence in SEQ ID NO:1. The invention also discloses a preparation method of the polypeptide, which comprises the following steps of: searching related protein sequences in a database; carrying out multiple alignment comparative analysis to screen out a highly conservative region for combination of a vMIP-II receptor; eliminating interference of unrelated residues through analysis simulation means; determining a target peptide sequence; synthetizing the active polypeptide by a solid-phase synthesis method; and finally testing the bioactivity of the polypeptide. The polypeptide can be used as a CCR5 receptor antagonist for the development of an anti-inflammatory drug, can be used as a lead drug for treating AIDS (Acquired Immune Deficiency Syndrome) and can also be used as a medicament with the immune regulation effect or a medicament for improving the functions of an immune system of an organism.

The invention relates to an adipose tissue composite preparation, and a preparation method and an application thereof. The adipose tissue composite preparation includes PDGF, umbilical cord mesenchymal stem cells, SVF and adipose tissues. The preparation method comprises the following steps: preparing a cell suspension from the SVF, the umbilical cord mesenchymal stem cells and the PDGF, and uniformly mixing the adipose tissues with the cell suspension according to a volume ratio of 20:1 in order to prepare the adipose tissue composite preparation. The adipose tissue composite preparation can be applied in plastic surgery or beauty treatment as a filling preparation. The composite biological preparation has the advantages of easiness in revascularization after being injected, and increased survival rate, is safe and reliable, allows the adipose tissues to be pale yellow, soft and bright and to be only wrapped with few fibers after being transplanted for 2-3 months; and the density of capillary tubes in the center position of transplanted fats is normal, and the blood vessel revascularization is obvious.

The invention discloses a method for inducing and differentiating precursor cells of a human skin source into corneal endothelial cells. The precursor cells of the human skin source are used and cultured together with the corneal endothelial cells B4G12, corneal endothelial cells theoretically approaching corneal endothelial cells of a normal person are successfully induced, the obtained corneal endothelial cells are applied to a corneal endothelial decompensation animal model, the corneal endothelium of an animal is successfully repaired, and the method has important clinical application prospects.

The invention discloses a recombinant rat phospholipase Cγ2 adenovirus, which is obtained by inserting the rat phospholipase Cγ2 gene into an adenovirus shuttle plasmid pHBAd‑MCMV‑GFP containing the adenovirus genome, the rat phospholipase Cγ2 gene The nucleotide sequence is shown in SEQ ID NO.1. The present invention also provides the construction method and application of the above-mentioned recombinant rat phospholipase Cγ2 adenovirus. Aiming at the promoting effect of PLCγ2 in cancer cell apoptosis, the present invention constructs a PLCγ2 recombinant adenovirus to replace the gene model, which can regulate the growth of cancer cells in a targeted manner, and the recombinant rat phospholipase Cγ2 adenovirus can stably express rat phospholipids Enzyme Cγ2, with high virus titer and significant pro-apoptotic effect; recombinant adenovirus and the rat phospholipase Cγ2 expressed by it can significantly inhibit cell proliferation and promote apoptosis of rat liver tumor cells, and can be used for the prevention of liver tumors And treatment provides new treatment ideas.

The invention relates to a method for detecting and diagnosing early-stage acute infection of toxoplasmosis by utilizing aptamers which are separately YZ-39 and YZ-56. The action mechanism of the aptamers is that the aptamers are specifically combined with a rhoptry secretory protein ROP of the toxoplasma gondii. The secretory protein ROP is the early-stage secretory protein of a toxoplasma gondii infected host cell, and is of great significance on diagnosing early-stage acute infection of the toxoplasmosis. At present, the report about detecting early-stage acute infection of toxoplasmosis by utilizing aptamers to detect the secretory protein ROP is not available at home and abroad. The discovery and application of the ROP specific aptamers provide a novel method for detecting the early-stage acute infection of the toxoplasmosis.

The invention discloses an extracting method of dog adipose-derived stem cells, and a preparation and application of the dog adipose-derived stem cells. The preparation for treating dog chronic nephrosis is prepared from the dog allogeneic adipose-derived stem cells. The extracting method of the adipose-derived stem cells comprises the steps: the dog abdominal adipose tissue is obtained, digesting, filtering and centrifuging are conducted, red blood cell lysis buffer resuspending is conducted, and the cells of P0-P3 generations are cultured; and the obtained adipose-derived stem cells of the P3 generation are subjected to surface antigen testing, the adipose-derived stem cells which have more than 70% of CD29 and MHC-1 expressions, less than 2% of CD34 and CD45 expressions, and the high differentiative potential, achieve adipogenesis, osteogenesis and chondrogenesis, and have normal chromosomes, feminine endotoxin and nont-detected mycoplasma are screened out and resuspended with the dosage of 1*10<6> cells/kg-5*10<6> cells/kg through normal saline of 0.5-1.0 mL to prepare the application of the dog allogeneic adipose-derived stem cells, and the preparation is used for treating dogchronic nephrosis. The stem cell preparation is weak in immunogenicity and is not involved in argument in the aspects of society, ethic and law; a transplanted person does not need to provide autologous stem cells, and nearly no damage is caused; and a separating method is simple, and the cells are very high in activity and easy to increase massively.