98 results about "Hirudo" patented technology

The invention relates to obstinate arthralgia wine used for treating rheumatic and rheumatoid arthritis arthralgia and a preparation method thereof and belongs to the field of medicine. The obstinate arthralgia wine is prepared with, by weight, nidus vespae, bee larva, eel blood, garter snake, long-noded pit viper, little multibanded krait, earthworm, centipede, Chinese azalea flower, red fennel root, four-leaf clover, prepared aconite root, prepared kusnezoff monkshood root, siegesbeckia herb, obscured homalomena rhizome, pepper stem, Chinese starjasmine stem, himalayan coralbean bark, scarlet kadsura root, clematis root, pawpaw, Wu Chia Pee, teasel root, cibotii rhizome, mulberry twig, pine node, wooly datchmanspipe herb, cassia twig, notopterygium root, pubescent angelica root, white peony root, geranium herb, silkworm excrement, beautiful sweetgum fruit, Chinese taxillus twig, drynaria rhizome, red-rooted salvia root, common peony root, logwood, ground beeltle, peach kernel, safflower, myrrh, zedoary, bidentate achyranthes root, frankincense, suberect spathololus stem, hirudo, Szechuan lovage rhizome, Chinese angelica root, hairy asiabell root, astragalus, glossy privet fruit, prepared rehmannia rhizome, sealwort, Chinese wolfberry, licorice and encommia bark. The obstinate arthralgia wine is rich in nutrition and excellent in taste, has efficacy of reinforcing qi and blood, tonifying liver and kidney, strengthening tendons and bones, dispelling wind and cold and removing dampness, meets drinking habits of people and can treat rheumatic arthralgia.

The invention relates to a hirudo culturing system which comprises a greenhouse 1 and a culture pond 2 arranged in the greenhouse 1. The greenhouse 1 is composed of a sun-resistant net 11 and supports 12; the culture pond 2 is formed by arranging multiple independent small ponds 3 in the longitudinal direction, and each small pond 3 is provided with a water inlet and a water outlet; a drainage ditch is formed in the side wall of the culture pond 2 in the longitudinal side; the top of the side wall of each small pond 3 is provided with a blocking net. According to the hirudo culturing system, the temperature can be effectively reduced and controlled at the temperature suitable for the hirudo growth, the hirudo culturing time is prolonged, it can be guaranteed that hirudo is cultured at least in 10 months each year, and the economic benefit of artificial hirudo culturing is increased.

The present invention discloses a hemiplegia coronary circulation promoting Chinese medicine preparation which is used for preparing apoplexy, which is prepared by the following components by weight: 100 parts of astragalus, 60-80 parts of campanumaea pilosula, 50-70 parts of angelica, 30-50 parts of chuanxiong rhizome, 30-50 parts of red paeonia, 40-60 parts of salvia root, 25-40 parts of safflower, 30-50 parts of dried tangerine fibres, 30-50 parts of earthworm, 20-30 parts of pangolin, 20-30 parts of buthus martensi karsch, 20-30 parts of giant typhonium tuber, 30-50 parts of batryticated silkworm, 40-60 parts of gastrodia elata, 30-50 parts of scutellaria, 30-60 parts of felwort, 0.6-0.8 parts of muskiness, 30-50 parts of medicinal cyathula root, 40-50 parts of loranthus mulberry mistletoe, 30-50 parts of acorus gramineus soland, 30-50 parts of arisaema cum bile, 30-40 parts of alum-processed pinellia tuber, 40-50 parts of dipsacus asper, 30-40 parts of prepared hirudo, 20-30 parts of rhubarb, 40-60 parts of hooked uncaria and 30-50 parts of abalone shell. The curative effect of Chinese medicine preparation is remarkable. The observation to 678 apoplexy patients shows that 583 patients are basically cured and the basic cure rate is not lower than 86%. The number of patients which are clearly better is 92 and the rate is not lower than 12%.

The invention discloses a technical method for breeding hirudo in pond net cages, which relates to the technical field of aquiculture, and comprises the steps: pond conditions and water requirements, making and setting of net cages, stocking of leech species, bait feeding, daily management and disease prevention. The technical method for breeding hirudo in the pond net cages has the beneficial effects that the technical method for breeding hirudo in the pond net cages fully utilize production resources, does not need professional equipment, does not have breeding pollution, is standard and normative in cultivating technology and science and standard in feeding, is suitable for breeding in large area ponds, is also suitable for small area breeding around every houses, is large in extended significance in the condition that the culture benefits are lower, and is excellent in prospect.

The invention relates to Zhuifeng wine used for treating rheumatic and rheumatoid arthritis arthralgia and a preparation method thereof and belongs to the field of medicine. The Zhuifeng wine is prepared with, by weight, 15g of Chinese angelica root, 15g of myrrh, 15g of frankincense, 15g of red-rooted salvia root, 15g of Szechuan lovage rhizome, 15g of white peony root, 15g of prepared rehmannia rhizome, 15g of prepared eucommia bark, 15g of medicinal cyathula root, 15g of cyperi rhizome, 15g of notopterygium root, 15g of Chinese parsnip root, 15g of pubescent angelica root, 15g of wooly datchmanspipe herb, 30g of acanthopanax root, 15g of garden burnet, 15g of pawpaw, 15g of cassia twig, 15g of white atractylodes rhizome, 15g of hairy asiabell root, 15g of long pepper, 15g of earthworm, 15g of poria, 15g of red date, 15g of hirudo, 15g of ground beeltle, 15g of teasel root, 15g of pseudo-ginseng, 15g of safflower, 15g of prepared monkshood, 9g of prepared aconite root, 9g of whole scorpion, 9g of cicada shell, 5g of Chinese wolfberry, 45g of prepared strychni seed, 30g of garter snake, 16g of centipede and 900g of Baijiu. The Zhuifeng wine is rich in nutrition and excellent in taste, has efficacy of chasing wind and activating collaterals and harmonizing blood and relieving pain, meets drinking habits of people, can treat rheumatic arthralgia and is medicinal wine effective in treating rheumatic and rheumatoid arthritis arthralgia.

The invention discloses a hirudin extraction separation technique which comprises the following steps: smashing and homogenizing hirudo to obtain an extracting solution, filtering through a ceramic filter membrane, and cleaning the ceramic filter membrane with distilled water; merging the ceramic filter membrane filtrate and cleaning solution, filtering through an organic filter membrane, and cleaning the organic filter membrane with distilled water; eluting the organic filter membrane filtrate and cleaning solution through a gel chromatographic column, and detecting the eluate by a high pressure liquid chromatograph; collecting the fraction with the hirudin chromatographical purity of 80% above, concentrating the collected eluate through a nanofiltration membrane, purifying the concentrated solution through a reverse-phase c18 semipreparative column, and collecting the target peak value; and carrying out freeze drying to obtain the freeze-dried hirudin pure product. By adopting the filtration and separation mode of combining the ceramic filter membrane and organic film, most impurities in the hirudo homogenate can be removed, thereby enhancing the extraction efficiency. The technique is convenient to operate, avoids the use of the organic solvent, and greatly lowers the extraction preparation cost.

The invention discloses an external traditional Chinese medicine composition for treating pain in neck shoulders low-back and legs and a preparation method and a using method of the external traditional Chinese medicine composition. The composition comprises the following components in parts by weight: 70-90 parts of Radix Notoginseng, 25-35 parts of Radix Aconiti, 25-35 parts of Radix Aconiti Kusnezoffii, 25-35 parts of Borneolum Syntheticum, 30-40 parts of Radix Dipsaci, 30-40 parts of Rhizoma Chuanxiong, 15-25 parts of Herba speranskiae tuberculatae, 15-25 parts of Herba ajugae ciliatae, 15-25 parts of Olibanum, 15-25 parts of Myrrha, 55-65 parts of Semen Sinapis Albae, 65-75 parts of Flos Carthami, 35-45 parts of Radix Angelicae Sinensis, 35-45 parts of Rhizoma Et Radix Notopterygii, 35-45 parts of Radix Angelicae Pubescentis, 35-45 parts of Cortex Eucommiae, 35-45 parts of eupolyphaga, 35-45 parts of Hirudo, 45-55 parts of Herba Lycopodii, 45-55 parts of Caulis Spatholobi and 45-55 parts of Rhizoma Corydalis. Blood circulation of human bodies is promoted, and effects of promoting blood circulation and dispelling blood stasis, and relieving pain are achieved; on the basis of the principle of 'pain caused by obstruction and dredging resulting in no pain', various Chinese herbal medicines cooperate, effects of promoting blood circulation to remove obstruction in the collateral, expelling wind and cold, improving nutrition and regulating equilibrium between yin and yang are achieved, and the health-care effect of preventing pain can be achieved. The using method of the external traditional Chinese medicine composition is simple, and the external traditional Chinese medicine composition takes effect quickly.

The invention provides a traditional Chinese medicine composition for treating lymphoma. The traditional Chinese medicine composition comprises the following active ingredients in parts by weight: 4-20 parts of dried tangerine peels, 3-12 parts of selfheal, 3-15 parts of radix astragali, 2-10 parts of herba corydalis bungeanae, 3-12 parts of atractylodes, 1-5 parts of centipede, 1-5 parts of indigo naturalis, 1-5 parts of bombyx batryticatus, 3-15 parts of fructus hordei germinatus, 3-12 parts of rhizoma paridis, 3-9 parts of angelica sinensis, 3-12 parts of solanum lyratum thumb, 1-8 parts of fructus forsythia, 3-15 parts of rhizoma anemarrhenae, 1-6 parts of hirudo, and 4-10 parts of radix scrophulariae. The traditional Chinese medicine composition integrates the effects of clearing away heat and toxic materials, removing stasis and dispersing swelling, eliminating dampness and eliminating phlegm, strengthening spleen and regulating qi, promoting blood circulation to remove blood stasis, and promoting qi circulation and relieving pain, thus inhibiting or killing cancer cells, eliminating cancer focus, improving the immunity of the organism, preventing the tumor metastasis and relapse, and being clinically used for treating lymphoma. According to the formula, the traditional Chinese medicine composition not only has a good curative effect on the lymphoma through experiment tests, but also is simple in formula and low in cost.

The invention discloses a recombinant bacterium for producing sulfo-recombinant hirudin as well as a preparation method and application of the recombinant bacterium, and belongs to the technical field of genetic engineering. In order to improve the synthesis yield of the sulfated recombinant hirudin and reduce the production cost, the invention provides the recombinant bacterium for producing the sulfo-recombinant hirudin, wherein the recombinant bacterium overexpresses a sulfotransferase gene and a hirudin gene, and an original strain is escherichia coli; and the nucleotide sequence of the hirudin gene is one of SEQ ID No.1 to SEQ ID No.3, and the nucleotide sequence of the sulfotransferase gene is as shown in SEQ ID No.4. According to the method, the activity of the recombinant hirudin is remarkably improved, a one-step purification method of the hirudin is realized, a recombinant hirudin product with relatively high purity can be obtained at one time, and the pressure of subsequent purification is greatly reduced.

The invention relates to a high-density artificial breeding method for poecilobdellamanillensis. The high-density artificial breeding method comprises the steps that 1 a breeding pool is constructed; 2 breeding substrate is placed; 3 seedlings are thrown in the breeding pool; 4 feeding is conducted; 5 leeches are separated into different pools; 6 water quality management is conducted; 7 disease prevention and disease treatment management are conducted. According to the high-density artificial breeding method for poecilobdellamanillensis, the high-density breeding condition of poecilobdellamanillensis can be controlled well, observation and management on the poecilobdellamanillensis are convenient, the disease prevention and disease treatment management of the leeches is strengthened, the high yield of 500-600 kg for per mu can be realized, and the economic benefit for artificial breeding of the poecilobdellamanillensis is increased.

The invention belongs to the field of hirudo culture, and particularly relates to an upward flow circulating water culture system for hirudo. The upward flow circulating water culture system comprises a water platform and a culture zone. The water platform comprises a support, a buoyancy tank and pedals, the support is formed in a longitudinal and transverse crossing manner, the buoyancy tank is arranged at the bottom of the support, and the pedals are fixed to the upper surface of the support; the culture zone comprises a drainage channel, a flow pushing pump and a plurality of culture tanks, the culture tanks are arranged on the support, and the drainage channel is arranged among the culture tanks. The upward flow circulating water culture system is characterized in that an inflation hole is formed in the buoyancy tank, and an air release valve is arranged on the buoyancy tank; the culture tanks comprise tank side walls and metal netted tank bottoms; first through holes are formed in the upper portions of the tank side walls; second through holes which correspond to the first through holes are formed in the side walls of the drainage channel, and the first through holes are fixedly connected with the second through holes by water outlet pipes; a drainage pipe is arranged on the lower portion of a side wall of the drainage channel and is connected with the flow pushing pump by a tee joint. The upward flow circulating water culture system has the advantages that water flow in the culture tanks can be effectively circulated and can be in flow states constantly, and accordingly the oxygen content of water can be increased.

A hirudin extracting method belongs to the technical field of hirudin extraction and comprises the following steps: 1), starving: 2), stimulating for excretion: holding ambient temperature to 23-40 DEG C, spraying an attractant to the body surface of a leech under ultraviolet irradiation at 200 nm and 275 nm in wavelength for 30-180 seconds, turning off ultraviolet, irradiating the leech for 3-15 min by using a warm white light in a flashing manner, or irradiating the leech for 3-15 min by using a warm white light and a green light in an alternating flashing manner such that the leech is stimulated to secrete a secretion; 3), sterilizing; 4), concentrating. The hirudin extracting method is high in hirudin extraction quantity and allows repeated extraction, the powdered hirudin finally obtained has light color and high level of active ingredients.

The invention discloses a traditional Chinese medicine composition for reducing blood lipid viscosity, a preparing method and application. The raw material components of the traditional Chinese medicine composition include chrysanthemums, gynostemma pentaphyllum, cistanche, schisandra chinensis, cloves, poria cocos, caulis polygoni multiflori, polygala tenuifolia, pinellia ternata, licorice, astragalus, radix paeoniae rubra, radix angelicae sinensis, flos carthami, lumbricus, salviae miltiorrhizae, hirudo, eucommia ulmoides, raw hawthorns, radix puerariae, scorpions, ginkgoes, erigeron breviscapus, rhizoma alismatis, semen ziziphi spinosae, semen cassiae, medicated leaven massa medicata fermentata, glossy privet fruits, honeysuckles, wild buckwheat rhizomes, yerbadetajo herbs, raspberry, linseed, juncus, reed rhizomes, lophatherum gracile, cortex albiziae and honey. The traditional Chinese medicine composition for reducing blood lipid viscosity, the preparing method and the application have the advantages that materials are easy to get, effects are obvious, no side effect exists, the preparing technology is traditional, the value of traditional Chinese medicine is reserved to the maximum, and the effect of reducing blood lipid viscosity is obvious.

The invention provides a medicine for prevention and treatment of postoperative lower extremity deep venous thrombosis. The medicine is characterized by comprising hirudo, suberect spatholobus stem, Danshen root, radix achyranthis bidentatae, dragon's blood, pseudo-ginseng powder, peach kernel, safflower, prepared rehmannia root, Szechuan lovage rhizome, red peony root, radix angelicae sinensis, radix astragali, roasted rhizoma atractylodis macrocephalae, tuckahoe, pilose asiabell root, coptis root, amur cork tree bark, Chinese atractylodes rhizome, honeysuckle, pawpaw and raw liquorice. Through verification of clinical research and experiment research, the medicine which is a preparation has excellent effects when being used for preventing forming of lower extremity DVT (deep venous thrombosis) after TKA (total knee arthroplasty), and through demonstration, a qi-benefiting blood-activating method is feasible and significant to DVT prevention and treatment, provides theoretical bases and clinical cases for further seeking efficient and safe traditional Chinese medicine therapy and also opens up new vision and develops new ideas for modern application advocating famous prescriptions.

The invention discloses a preparation method of cancer analgesic patch, prepared in following steps: (1), mixing and pulverizing, by weight, Sichuan lovage rhizome 30-86 parts, ground beetle 15-35 parts, unprocessed Radix Aconiti 5-78 parts, gamboge 20-89 parts, unprocessed Radix Aconiti Kusnezoffii 15-98 parts, gecko house lizard 13-68 parts, Rhizoma Dioscoreae Bulbiferae 21-78 parts, centipede 15-76 parts, Semen Strychni 15-98 parts, Hirudo 13-98 parts, bulb of edible Tulip 23-88 parts, frankincense 18-78 parts, unprocessed Chinese Stellera root 15-98 parts, myrrh 13-68 parts, unprocessed Rhizoma Arisaematis 17-89 parts, Galla chinensis 18-78 parts, Radix Euphorbiae Pekinensis 15-79 parts, realgar 12-78 parts, dragon's blood 12-78 parts, and borneol 8-65 parts to obtain a medicinal powder; mixing, by weight, the medicinal powder 50 parts, Vaseline 30 parts and bee honey 20 parts to obtain paste, and applying the paste to obtain the patch. The patch with the finely selected twenty Chinese herbs is effective in softening hardness to dissipate stagnation, eliminating toxin to arrest pain, dredging channels and dispersing blood stasis to eliminate symptoms, is free of addiction or independence, has significant, lasting analgesic effect and is free of side effect.

The invention belongs to the technical field of traditional Chinese medicines, which aims at solving the technical problems in the prior art that wind-cold-dampness ostalgia as well as swelling and accumulation of various parts all over the body are difficult to heal and the existing external application agent takes effect slowly. The invention provides a composition for external use for dispelling wind, eliminating dampness, and clearing and activating channels and collaterals and an application method of the composition. The composition is prepared from the following crude drugs in parts by weight: 25 to 50 parts of herb of asarum heterotropoides, 25 to 40 parts of rhizoma seu radix notopterygii, 25 to 50 parts of radix saposhnikoviae, 25 to 48 parts of radix gentianae macrophyllae, 25 to 49 parts of rhizoma sparganii, 25 to 49 parts of rhizoma curcumae, 25 to 45 parts of hirudo, 25 to 35 parts of draconis sanguis, 25 to 30 parts of centipede, 25 to 32 parts of bungarus parvus, 25 to 37 parts of olibanum, 25 to 37 parts of myrrha, 25 to 31 parts of eupolyphaga steleophaga, 25 to 50 parts of Chinese angelica, 25 to 50 parts of rhizoma chuangxiong, 25 to 50 parts of rhizoma atractylodis, 25 to 50 parts of cinnamomi ramulus, 25 to 33 parts of flos carthami, 25 to 50 parts of herb of phryma leptostachya, 25 to 34 parts of peach kernels, 25 to 50 parts of semen coicis, 25 to 36 parts of zaocys dhumnade, 25 to 36 parts of scorpio, 25 to 50 parts of radix angelicae pubescentis, 25 to 50 parts of radix et rhizome rhei and egg white. By utilizing a guiding drug effect of the egg white, active ingredients of various drugs can directly reach an affected part, so that the drugs can take effect faster, and the effect is significant.

The invention relates to the technical field of hirudo processing, in particular to a tray for processing officinal hirudo. The tray is an uncovered latticed frame body, sliding wheels which enable the tray to slide conveniently and are distributed symmetrically are arranged on the bottom of the outer surface of the tray, and the sliding wheels are movably connected to the bottom of the tray through bearings and bolts. One to five layers of form boards with an identical size are arranged inside the tray from bottom to top, the size of the form boards is matched with the size of the bottom of the tray, and a lime net layer cover plate is arranged on the upper portion of each form board. Bar-shaped grooves are evenly distributed in the bottom of each form board, and liquid discharging ports are formed in the lowest parts of the bottoms of the bar-shaped grooves. Compared with the prior art, the tray for processing the officinal hirudo is simple in structure, the arrangement of the lime net layer cover plates can prevent the hirudo from running away everywhere when the hirudo feels high external temperature, the design of the bar-shaped grooves in the bottoms of the form boards can prevent the hirudo from being killed due to scald or forming a curl state, final hirudo dry products are made to present a tabular fusiform shape, subsequent processing can be carried out conveniently, and the tray for processing the officinal hirudo is suitable for large-scale popularization and application.

The invention discloses a hirudo cultivation device which comprises a bottom and an enclosure wall, wherein the enclosure wall extends upwards along the edge of the bottom and the upper end of the enclosure wall is opened. The inner wall of the enclosure wall is provided with a plurality of steps, and the steps extend to the upper end portion of the enclosure wall from the edge of the bottom in a stair mode. By the adoption of the technical scheme, the hirodo cultivation device has the advantages that hirudo is convenient to take and eat, cultivation density is large, and stocking amount is large. According to the optimized technical scheme, the perpendicular distances D1 between adjacent steps are equal, and the horizontal widths D2 of the steps are equal, so that the interior of the hirudo cultivation device is uniform and in order, and convenient to manufacture and manage by cultivation staff.

The invention discloses a Chinese medicinal composition for preventing heart and cerebral thrombosis diseases, and is combined according to an assistant and guide formula principle, wherein sovereign drugs comprise ginseng and astragalus, minister drugs comprise hedyotis diffusa, bombyx batryticatus, centipede, hirudo, eupolyphaga, earthworm and scorpio, assistant drugs comprise panax notoginseng, rhizoma chuanxiong, salviae miltiorrhizae, flos carthami, peach seeds and puerarin, and conductant drugs comprise radix curcuma, ramulus euonyrni and luffa. The Chinese medicinal composition for preventing heart and cerebral thrombosis diseases skillfully uses taste of meridian tropism, is more scientific than a common formula compatibility, and does not have rare medicinal materials, thereby being economic in cost and also being capable of accurately preventing the heart and cerebral thrombosis diseases, and having excellent promoted application prospect.

The invention discloses a preparation method and application of a biological recognition probe for capturing hirudin polypeptide. Ligands are modified on the surface of a magnetic solid-phase carrier,thrombin is indirectly fixed, the biological activity is reserved to a great extent, and the obtained probe can be combined with multiple detectors to achieve extraction and determination of the hirudin polypeptide, and has the advantages of being high in selectivity, rapid, sensitive, good in stability, wide in applicability, small in interference, high in repeated utilization rate, environmentally friendly and the like.

The invention provides a drug for treating rheumatic arthritis through oral taking and external application and a preparation method of the drug. The drug comprises an orally-taken drug and an externally-applied drug, wherein the orally-taken drug comprises the following raw medicinal materials: radix morindae officinalis, rhizoma drynariae, achyranthes bidentata, radix gentianae macrophyllae, ground beetle, angelica, radix paeoniae alba, radix clematidis, wooly datchmanspipe herb, liquorice, radix angelicae pubescentis, cassia twig, radix saposhnikoviae, radix bupleuri, white mustard seeds, bombyx batryticatus, hirudo and scolopendra; the externally-applied drug comprises the following raw medicinal materials: common achyranthes herb, root and rhizome of ripqrian greenbrier, all grass of serrulate clearweed, Japanese stephania root, rhizome of largeleaf Japanese ginseng, vanilla, herba polygoni orientalis, herb of tuberculate speranskia, caulis erycibes, herba lycopodii and root or all-grass of cairo morningglory. The drug has the beneficial effects that by being orally taken and externally applied, the drug has a definite treating effect to the rheumatic arthritis and is low in toxic and side effects.

The invention belongs to the technical field of health-care products, and in particular relates to a life prolonging and beauty maintaining health-care product and a preparation method thereof. The life prolonging and beauty maintaining health-care product is prepared from the following active ingredients: sheep placenta, cordyceps sinensis, rhizoma atractylodis macrocephalae, radix astragali, panax notoginseng, fructus crataegi, radix salviae miltiorrhizae, poria, fructu broussonetiae, red kojic rice, herba cistanche, radix polygoni multiflori, radix angelicae sinensis, radix rehmanniae, folium ginkgo, borneol, fruit of Chinese magnoliavine, ginseng, stigma croci, hirudo powder, radix paeoniae rubra, radix scrophulariae and sugar. The life prolonging and beauty maintaining health-care product is scientific in prescription, simple to prepare, free from toxic and side effects and free from residues, and the life prolonging and beauty maintaining health-care product is capable of implementing internal adjusting and nourishing, delaying aging, boosting body immunity, eliminating sub-health, reducing diseases and improving overall mental state.

The invention relates to a product for patients with aseptic necrosis of femoral head, belonging to the field of traditional Chinese medicine. The product is mainly prepared from the following raw materials in parts by weight: 100-150 parts of Cornu Cervi Pantotrichum, 90-150 parts of Drynaria fortunei, 40-90 parts of Cucumis melo seed, 50-100 parts of Dipsacus asperoides, 60-100 parts of Acanthopanax gracilistylus bark, 50-100 parts of Cortex Eucommiae, 50-90 parts of Radix Astragali, 40-100 parts of Radix Codonopsis, 40-80 parts of processed rhizome corydalis, 30-80 parts of Radix Paeoniae, 30-60 parts of Carthamus tinctorius flower, 30-80 parts of Angelica sinensis, 40-80 parts of Eupolyphaga seu steleophaga, 30-60 parts of Acacia catechu, 30-80 parts of calcined pyrite, 30-70 parts of hirudo, and 10-30 parts of Radix Glycyrrhizae. The product provided by the invention has a unique formula, convenient preparation method, quick action, and is suitable for patients with aseptic necrosis of femoral head caused by traumata, hormone, long-term drinking and the like. Through clinical curative effect observation of more than 1,000 cases in 10 years, the curative ratio is up to 90%, and the effective rate is 95%.

The invention discloses a leech tyrosine sulphonate transferase gene and application thereof, and belongs to the field of biomedicine. The leech tyrosine sulphonate transferase gene is characterized in that on the basis of a hirudo nipponia transcriptome database, a leech tyrosine sulphonate transferase cDNA sequence is excavated and identified through a bioinformatics means, and on this basis, the leech tyrosine sulfotransferase is synthesized through codon optimization, gene synthesis and microbial cell expression, and the recombinant hirudin is catalyzed by the leech tyrosine sulfotransferase to be subjected to sulfonation modification to obtain the natural hirudin. The catalytic ability of the transferase is greatly improved compared with that of acyl sulfonate transferase, human-derived tyrosine sulfonate transferase and bovine-derived tyrosine sulfonate transferase. Compared with other tyrosine sulfonic acid transferase, the leech tyrosine sulfonic acid transferase obtained in the invention has stronger substrate preference for recombinant hirudin, is suitable for large-scale preparation of natural hirudin, and provides a new way for synthesis of natural hirudin.

The invention discloses a traditional Chinese medicine composition for treating diabetes and a preparation method thereof. The composition is composed of 19 traditional Chinese medicines including: fresh porcine pancreas, white muscardine silkworm, hirudo, endothelium corneum gigeriae galli, American ginseng, panax notoginseng, balsam pear, schisandra chinensis, lance asiabell roots, fructus amomi, walnut kernels, fructus lycii, rhizoma atractylodis, black soybean, hawthorn, astragalus membranaceus, wild black fungus, kudzu vine root and Chinese yam. According to the invention, a very critical and essential object in traditional Chinese medicine is really achieved. Not is blood glucose simply reduced but a damaged pancreas is repaired. A sufficient amount of insulin can be secreted; excessive sugar in blood can be neutralized; hypercoagulable state can be improved; microcirculation is unblocked; and complications in a diabetic patient is reduced or alleviated.