201 results about "Camptotheca" patented technology

Disclosed are compositions and corresponding methods of their use that include extracts from Loropetalum chinensis, Camptotheca acuminate, Lonicera maackii, Washingtonia filifera, Artemisia parviflora, Glochidion lanceolaris, Polygonum hydropiper, Populus davidiana, Tsoongiodendron odorum, Trema angustifolia, Ficus tikoua, Pistacia chinensis, Zizyphus mauritiana, Garuga forrestii, Michelia figo, Sabina chinensis, Cuphea balsamona, Setaria palmifolia, Polygonum lapathifolium, Machilus longipedicellata, Geranium nepalense, Ipomoea obscura, Cedrus deodara, Quercus aliena, Carqueja, Condurango, Catuaba, Carex baccans, Chrysalidocarpus lutescens, Gnaphalium pensylvanicum, Celtis sinensis, Cassia siamea, Catalpa yunnanesis, Potamogenton perforliatus, Cinnamomum japonicum, or any combination thereof.

The invention discloses a botanical pesticide and a preparation method thereof. The botanical pesticide contains effective ingredients extracted from tripterygium wilfordii hook f, henbane, aleurites fordii and camptotheca acuminate. The botanical pesticide has the advantages of simple components, convenient use, no pollution and residual on grain crops, low toxicity on human and animals, difficult generation of pesticide resistance, good and broad-spectrum insecticidal effects, good control and killing effects on multiple insects which can damage respectively multiple grain crops, fast generation of effectiveness and long effective duration. The preparation method has the advantages of simple process, easy control on conditions, good yield of active ingredients of raw materials, and goodproduct performances of stable ingredients and easy preservation and use. The invention also provides a use method of the botanical pesticide. The use method comprises diluting the botanical pesticide and spraying the diluted botanical pesticide on a leaf surface. The use method is convenient for implementation.

The invention provides a biological plant source miticide prepared from urban greening wastes, and preparation and application of the biological plant source miticide. The biological plant source miticide is composed of 40%-80% of bulk drugs and 20%-60% of conventional auxiliary materials. The bulk drugs comprise the following components in percenrage by weight: 2%-20% of ligustrum lucidum extract, 5%-30% of ailanthus altissima extract, 5%-30% of liriodendron chinensis extract, 5%-10% of camptotheca acuminate extract, 5%-25% of magnolia grandiflora linn extract, 5%-30% of koelreuteria paniculata extract, 5%-20% of cinnamomum camphora extract, 5%-15% of sapium sebiferum extract and 4%-10% of berberis thunbergii DC. extract. The plant extracts are all obtained by drying, powdering, leaching and filtering branches, stems, leaves, flowers and fruits or mixtures of these parts, which are cut from plants, or shed and wasted naturally or wasted in the process of processing other products. The biological plant source miticide is efficient, free from residuals, low in production cost, high in combination property as well as economical, practical and environment-friendly.

The invention relates to a preparation method of an enzymatic cross-linking medicine carrying nano micelle. The preparation method comprises the following steps: carrying out self-assembling to prepare a polymer micelle system by using a thermosensitive amphiphilic block copolymer, introducing a phenol group capable of generating cross-linking reaction into a thermosensitive block, catalyzing peroxidized hydroxide phenol group by Peroxidase to generate cross-linking reaction to obtain a nuclear crosslinking polymeric micelle, wherein the lowest critical micelle concentration is remarkably lower than that of nano micelle which is not cross-linked; when the micelle is prepared, adding adriamycin, taxol or camptotheca acuminate to prepare the enzymatic cross-linking medicine carrying nano micelle. The preparation method provided by the invention has the advantages that compared with conventional cross-linking methods, the preparation method has the advantages of safety, no toxicity, quick cross linking and the like, and is simple and convenient to operate, and has a good application prospect; the enzymatic cross-linking medicine carrying nano micelle has lower critical micelle concentration, so that the stability is improved, the circulating time of the micelle in blood can be prolonged and the efficiency that the micelle is endocytosed by tumor cells is increased, thereby further improving the bioavailability and the therapeutic effect of the medicine.

The invention discloses a method for extracting betulinic acid, hydroxycamptothecin and camptothecin from a camplotheca acumiate fruit in a clean way, which takes the camplotheca acumiate fruit as raw materials and extracts active ingredients by using the methods of polyamide column chromatography and ethanol elution. The device required by the method is simple and the whole extracting and separating process is environment friendly by using ethanol to elute, the whole process needs only 60 percent of the ethanol, the consumption of a desorption agent is few, the materials are obvious to segment, the method is simple and easy, the ethanol can be recycled, thus greatly lowering the cost and being applicable to relatively larger scale preparation, having relatively higher application value and excellent social benefits and values, and being significant in making full use of the camplotheca acumiate fruit of China.

The invention provides a compound plant pesticide capable of controlling plant aphid, and preparation and application thereof. The pesticide is composed of 40 to 80% of bulk drugs and 20 to 60% of conventional accessory materials. The bulk drugs are composed of, by weight, 2 to 20% of tomato extract, 5 to 30% of garlic extract, 5 to 30% of extract of euphorbia humifusa, 5 to 10% of ginger extract, 5 to 25% of potato extract, 5 to 30% of extract of cinnamomum camphora, 5 to 20% of extract of Chinese photinia, 5 to 15% of tulip tree extract and 4 to 10% of extract of camptotheca acuminate. The pesticide is originated from natural plant extract, easily degradable under natural conditions, safe to the environment and surrounding ecological systems and free of environmental pollution; and the pesticide is a nuisance-free plant-derived biopesticide.

The invention discloses a method for increasing content of camptothecin in camptotheca hairy root in the technical field of biology. The method comprises the following steps of: cloning coding frame sequences of CrORCA3 and CrG10H genes from catharanthus roseus; constructing a plant bivalent high-efficiency expression vector including the genes; and genetically transforming the camptotheca into camptotheca hairy root genetically modified by CrORCA3 and CrG10H genes, by using the hair root agrobacterium-mediated technique. The camptothecin content in the obtained genetically-modified camptotheca hairy root is increased obviously. The content of the camptothecin is 5.4 times of the contrast. The content of hydroxycamptothecin is 2.3 times of the contract. The content of the hairy root camptothecin independently genetically-modified by CrORCA3 is increased by 1.5 times of the contract. The content of the hairy root camptothecin independently genetically-modified by CrG10H gene is not increased obviously. The invention provides a method for increasing content of the camptothecin in the camptotheca hairy root, thereby providing a novel method for producing the camptothecin and the hydroxycamptothecin as anti-cancer drugs of important clinical requirements.

The invention provides a pest-repelling and bacterium-inhibiting fertilizer prepared from Chinese herbal medicine extract. The fertilizer is characterized in that the fertilizer comprises, by weight,50-230 parts of urea, 50-130 parts of monoammonium phosphate, 50-400 parts of potassium nitrate, 10-200 parts of mineral source potassium fulvate and 10-100 parts of the Chinese herbal medicine extract, the Chinese herbal medicine extract is prepared by mixing Chinese herbal medicines according to a certain ratio and extracting the obtained mixture, and the Chinese herbal medicines comprise tartary buckwheat, licorice root, Chinese Gall, kelp, Tamarix chinensis, seaweed, Figwortflower Picrorhiza Rhizome, Rhizoma Cibotii, fructus cnidii, neem seeds, anise, Areca catechu, pomegranate rind, RadixAucklandiae, Radix Asparagi, Sophora flavescens, Vietnamese Sophora Root, Chinese prickly ash, Kaempferia galanga L., Camptotheca acuminate, Radix Euphorbiae Ebractealatae, Rhizoma curcumae longae, Rhizoma Arisaematis, Cynanchum paniculatum, oysters, seashells, crab shells, shrimp shells, oyster shells, tangerine peel, orange peel and Herba Houttuyniae. The provided pest-repelling and bacterium-inhibiting fertilizer prepared from Chinese herbal medicine extract has no damages to crops, can supplement nutrients required for plant growth and efficiently repel pests and inhibit bacteria, and caneffectively solve the problems in the background art.

The invention provides an external application traditional Chinese drug for treating psoriasis vulgaris. The external application traditional Chinese drug is a agent prepared from the following raw materials, by weight, 5-9 parts of densefruit pittany root bark, 5-9 parts of japanese ampelopsis root tuber, 5-9 parts of lightyellow sophora root, 9-15 parts of fructus kochiae, 5-9 parts of pinellia, 2-5 parts of amur corktree bark, 3-9 parts of common cnidium fruit, 1-5 parts of alum, 6-9 parts of stiff silkworm, 2-4 parts of chaulmoogratree seed, 9-15 parts of dried alum, 6-12 parts of mirabilite, 10-30 parts of wild buckwheat rhizome, 1-4 parts of glaber herba sarcandrae inj, 3-9 parts of japanese pogodatree flower, 2-6 parts of kansu euphorbia root, 9-15 parts of sulphur, 6-9 parts of kaempferia galanga, 3-9 parts of pomegranata rind, 6-15 parts of common bletilla tuber, 3-9 parts of podophyllum, 3-9 parts of siberian cocklebur fruit, 6-12 parts of smoked plum, 9-15 parts of flatspine pricklyash, 6-9 parts of psoralea corylifolia, 3-9 parts of common camptotheca fruit, 9-15 parts of Chinese soapberry fruit, and 1-30 parts of Chinese azalea flower. According to the traditional Chinese drug preparation of the present invention, the traditional Chinese drug five element theory is adopted, the medicine is applied aiming at the symptoms according to the psoriasis vulgaris etiopathogenisis, and the external application traditional Chinese drug has effects of heat clearing, detoxification, blood activating, and collateral dredging, and is suitable for more than 99% psoriasis vulgaris patients.

The high-effective production method of 10-hydroxycamptothecin includes the following steps: making seed of camptotheca acuminate undergo the processes of negative pressure cavitation mixed-rotating wall-breaking, disinfecting, removing impurity, water culture and germination, mixing the seed sprout and extraction solvent, homogenization and solid-liquid extraction, filtration, negative pressure cavitation mixed-rotating solid-liquid extraction, filtration, concentration, negative pressure cavitation mixed-rotating liquid-liquid extraction, recrystallization or silicon gel column chromatography so as to obtain the 10-hydroxycamptothecin. Said invention raises its yield, can be substituted for traditional various extraction methods of drying material, pulverizing and heating process.

The Chinese herbal medicine is used to treat various hemopathy, including aplastic anemia, anemia, thrombocytopenia, myelosis type hemocytopenia, leukaemia, etc. It incldues decoction and medicine powder, the decoction is prepared with angelica, astragalus root, lactiflora peony root, Chinese yam, sealwort and other seven kinds of Chinese medicinal materials; and the medicine powder is prepared with notoginseng, cucumber root, common camptotheca leaf and other three kinds of Chinese medicinal materials. The medicine has the functions of strengthening body resistance, eliminating evil, benefiting Yin and Yang, promoting vital energy and blood formation, raising the englobing function of macrophage, etc. and thus high curative rate.

The invention discloses a new strain capable of producing camptothecin and a method for preparing camptothecin by using the same. The new strain capable of producing the camptothecin-Phomopsis sp. B29 is conserved in China General Microbiological Culture Collection Center (CGMCC) on July 14, 2010, and has a conservation number of CGMCC No. 3988. The camptothecin is indole alkaloid with antitumor activity and is extracted from Camptotheca acuminate-nyssaceae plant of China, and the camptothecin has good curative effect on malignant tumors such as colon cancer, ovarian cancer and the like. Direct extraction of the camptothecin from the Camptotheca acuminate causes vegetation deterioration, ecological unbalance and other problems, and a chemical synthesis method has the disadvantages of long route, low yield and high cost; and aiming at the problems, endophytic fungi are separated and purified from the bark and fruit of the Camptotheca acuminate, and the strain capable of producing the camptothecin is screened from the endophytic fungi and then is used for preparing the camptothecin through fermentation. The new strain provided by the invention can be taken as a new medicine source for producing the camptothecin; and the method for preparing the camptothecin by fermenting the new strain is convenient to operate and has the advantage of low cost.

The invention provides 20(S)-camptothecin ramification demonstrated by the figure (1) or its acceptable salt in the medicine. The m in the figure is one integer between 2 and 5; R is OH, OR1 (R1 is the alkyl of C1-5), NH2, NHR1 (R1 is the alkyl of C1-5) or NR1R2 ((R1 and R2 are the alkyl of C1-5 respectively). The invention also provides the preparation method of the ramification and its use in the antineoplastic medicines.

The invention relates to a method for promoting growth of camptotheca acuminate. The method comprises the operating steps of obtaining a methyl alcohol extracting solution concentrate of branches and leaves of taxus chinensis var mairei, and irrigating the camptotheca acuminate with a water suspension of the methyl alcohol extracting solution concentrate. The method for promoting growth of the camptotheca acuminate is simple and high in operability, and by the adoption of the method, the whole plant biomass of the camptotheca acuminate can be improved by more than 18%, and the content of camptothecin in the camptotheca acuminate is increased by more than 12%.

The invention discloses an anticancer fragrance-increasing cigarette holder. A cigarette holder tube (2) and a suction nozzle (3) are of an integrated structure; one end of a cigarette insertion opening (1) is inserted into the outer end of the cigarette holder tube (2); both ends of the cigarette holder tube (2) are separated by a filtration net (4) to form a filtration region; and an anticancer fragrance-increasing composition for cigarettes is filled in the filtration region and contains the following components: 2 to 3 parts of taxus chinensis, 0.5 to 1 part of curcuma zedoary, 0.5 to 1 part of tears, 0.5 to 1 part of catharanthus roseus, 0.5 to 1 part of cephalotaxus fortunei, 0.5 to 1 part of disporum leschenaultianum, 0.5 to 1 part of rabdosia rubescens, 0.5 to 1 part of meadow saffron, 0.5 to 1 part of camptotheca acuminate, and 0.5 to 1 part of Java brucea. The anticancer fragrance-increasing cigarette holder facilitates the filling and exchange of the anticancer fragrance-increasing composition for cigarettes, so the harm from carcinogenic substances to human organs can be greatly reduced and the harm from smokes to the human bodies is reduced to the minimum; moreover, instead of damaging the original taste of the cigarette by simply absorbing a large amount of smoke, the anticancer fragrance-increasing cigarette holder makes the taste of the cigarettes more purer and easily accepted by adding a large quantity of plant spices.

The invention discloses a light regulation and control method for tissue culture and multiplication of camptotheca acuminata calluses, which sieves an optimal culture medium formula for establishing a camptotheca acuminate clone. A result shows that an optimal induction culture medium of stems contains MS, 0.1mg / L of KT and 4mg / L of 2,4-D; the optimal multiplication conditions are as follows: B5, 0.5mg / L of KT, 1.0mg / L of NAA (Naphthalene Acetic Acid) and 0.5mg / L of the 2,4-D; leaves represent poor inductivity. The calluses are multiplied under the conditions of red light, green light, blue light, red-blue light and white light; by analyzing the increment of calluses in unit, the red light and the green light have an acceleration effect on the growth of the calluses, the blue light has the inhibition effect on the growth of the calluses, and the effects of the white light and the red-blue light are not obvious; the acceleration effect of the green light is the most significant.

The invention relates to a vagina tightening and caring agent in gyniatrics, belonging to Chinese herbal medicine products. The raw materials for preparing the agent are as follows according to different weight proportion: lightyellow sophora root, phellodendron bark, wild chrysanthemum flower, dandelion, common cnidium fruit, borneol, Szechwan chinaberry fruit, honeysuckle, new Zealand spinach, ground beetle, angelica sinensis, common camptotheca fruit, curcuma, belvedere fruit, black nightshade, Rhizoma Zedoariae, liquorice, garden balsam, pomegranate rind, chebulae fructus, keel, purslane, myrrh, pearl, Chinese yam, rosemary, jade beauty, herba houttuyniae, leonurus japonicus, ligustrum lucidum, and salt teasel root. The gynecological vagina tightening and caring agent has the advantages of multiple effect, convenient use, rapid therapeutic effect, obvious effect, safe use, no poison and side effect, ability to improve immunocompetence and antiviral ability, cure gynecological diseases and prevent aging, and applicability to treatment of gynecological diseases, vagina tightening and ovary caring.

The invention relates to a method for efficiently extracting a camptothecin derivative. The method comprises the following steps of: mixing degreased common camptotheca fruits and dried common camptotheca leaves, homogenating and extracting; and freshly grinding, mixing and homogenating common camptotheca seed buds and fresh common camptotheca leaves according to a stress biology principle so as to improve the output of the camptothecin derivative in the raw materials. On the basis of the method, a new high-efficiency extracting and purifying process of camptothecin and 10-hydroxy camptothecin is obtained through applying serial innovation researches of negative-pressure cavitation mixed rotating high-efficiency strengthened leaching, negative-pressure cavitation liquid-liquid mixed rotating extracting, recrystallizing, preparation type HPLC(High Performance Liquid Chromatography) refining, and the like.

The invention belongs to the field of traditional Chinese medicine for treating tumor and provides a traditional Chinese medicine for treating gastric cancer. The aim of the traditional Chinese medicine is to have good effect and to take effect rapidly; the traditional Chinese medicine is prepared from oldenlandia diffusa, panaxnotoginseng, mastic, chilopod, glossy ganoderma, myrrh, goldhair hedyotis herb, borneol, climbing nightshade, dried rhizome of rehmannia, agrimony, angelica, common camptotheca fruit, fumitory and other traditional Chinese medicines; and the traditional Chinese medicine has the effects of strengthening body resistance, cultivating constitution, nourishing qi and blood, activating blood, removing stasis, clearing away heat, detoxicating and resisting cancer, has good effect, takes effect rapidly and has the advantages of remarkable effect and rapider effect taking.

The invention discloses an application of vincoside lactam extracted from common camptotheca fruit in preparation of antibacterial or antiviral or anti-hyperthermia or anti-inflammatory or pain relieving drugs or in preparation of drugs for treating infectious diseases caused by sensitized bacteria and viruses or leptospirosis. The diseases caused by sensitized bacteria and viruses comprise: acute tonsillitis, faucitis, upper respiratory tract infections, bronchitis, otitis media pneumonia, conjunctivitis, hordeolum, periodontal abscess, burn wound infections, urinary tract infections, and infections caused during after-surgery prevention. The invention also discloses a vincoside-lactam preparation with the application.

The invention belongs to the medicine field, particularly relates to a pharmaceutical composite for curing colon cancer and a preparing method thereof. The technical problem solved by the invention is to provide a brand-new pharmaceutical composite for curing colon cancer and a preparation method thereof. The pharmaceutical composition comprises the following components: 5-11 parts of rucea javanica, 5-11 parts of common camptotheca fruit, 8-16 parts of semen coicis and / or sargentgloryvinestem, 8-16 parts of curcumaaromaticaSalisb, and 5-16 parts of gingseng and / or campanumaea pilosula. The pharmaceutical composition has efficacies of clearing away heat and toxic material, activating blood circulation, removing masses, benefiting vital energy and strengthening healthy energy and the like.Long-term clinic observation and series of experimental researches show that the pharmaceutical composition has good anti-cancer effect, especially has obvious curative effect. The parasitical composition mainly cures patients of colon cancer suffering from toxin and phlegm internal resistance and qi deficiency, has the symptoms of abdominal pain, fixed pain spot, unpalpable pain, abdominalmass, abdominal distention, acratia, anorexia, dark-purple tongue or tongue with blockage points or ecchymosises, white and greasy tongue coat, and pulse fine tart, and is used by patients of colon cancer or colon cancer postoperation dialectical species suffering from toxin and phlegm internal resistance and qi deficiency.

The invention relates to the natural active ingredient extraction field from plants, in particular to a method for separating and extracting camptothecin. The technological line of the invention is as follows: common camptotheca fruits and common camptotheca leaves are taken as materials, and the technological steps are as follows: crushing, leaching of dilute sodium hydroxide solution, acid precipitation and impurity removal of the leaching solution, micro-filtration, ultrafiltration, nanofiltration, column chromatography by macropore adsorbent resins, desorption by ethanol and condensation of a stripping liquid to an extractive, extraction by ethyl acetate and crystallization of a extraction liquid to obtain a coarse product of camptothecin,recrystallization of a mixing liquid of the ethyl acetate and the acetone to obtain refined products of camptothecin. The method for separating and extracting camptothecin of the invention can fully leach out active ingredients of materials by multiple leaching, and after the processes of micro-filtration, ultrafiltration, nanofiltration concentration, column chromatography by macropore adsorbent resins and multiple crystallization, the extraction rate of the invention is 70 to 80 percent against the content of active ingredient camptothecin of the materials, and the purity of products extracted is more than 98 percent.

Belonging to the technical field of pest control, the invention provides a repellent for ant control. The repellent is prepared by the method of: 1) in terms of weight part, weighing 30-40 parts of radix aucklandiae, 25-35 parts of lily magnolia, 15-30 parts of common camptotheca fruit, and 5-15 parts of Derris trifoliate for standby use; 2) drying the radix aucklandiae, lily magnolia, common camptotheca fruit and Derris trifoliate, then chopping them into a mixed raw material, conducting smashing extraction on the mixed raw material with hydrous ethanol to obtain a mixed extracted solution, and performing flash concentration to 1 / 2-1 / 3 of the original volume to obtain an extract concentrated solution, i.e. the finished product. The repellent for ant control has natural raw materials, and all active ingredients of the components supplement each other and have a synergistic effect. The repellent can repel ants effectively, can affect mating and oviposition of ants, has good repellent effect on ants, and has the advantages of biodegradability, environmental friendliness, convenient use and safety.

The invention discloses a method for extracting and separating camptothecin from the tissues of a camptotheca, such as fruits, leaves and the like, which comprises the steps of: A. the extraction of camptothecin crude products; B. the preparation of semi-finished products by the separation and purification of the camptothecin crude products; and C. the preparation of products by the concentration and crystallization of the camptothecin semi-finished products. The invention can improve the content of the camptothecin to more than 99 percent, reduce the contents of single related impurities, such as 10-hydroxycamptothecine, 20-ethenylcamptothecin and 9-anisylcamptothecin and the like, to less than 0.1 percent, and has the advantages of high yield, large production, low cost, small pollution and being suitable for industrial production.

The invention discloses a plant pesticide for preventing vegetable grey mould. The plant pesticide is characterized by comprising, by weight, 20-30 parts of Siberian cocklebur, 20-30 parts of scutellaria roots, 15-20 parts of common camptotheca barks, 15-20 parts of yellow cinnamon leaves, 15-20 parts of clove, 4-8 parts of surface active agents, 6-10 parts of wetting agents and 80-100 parts of fillers. Raw materials of the pesticide are derived from plants and synergistically inhibit bacteria, the prevention effect on the vegetable grey mould reaches 83.6-92.7% and is remarkable, residues and drug resistance are avoided, and the pesticide is green, environmentally friendly and convenient to use.

The invention discloses a traditional Chinese medicine combination that is used for curing tumor, which comprises the following raw medicinal materials: camptotheca acuminate decne, chuanposhi radix cudraniae, pedate pinallia jackinthepulpit rhizome, yellow avens, radix curcumae, Chinese Paris rhizome, tassel flower, trachycarpus fortunei H. wendl, water caltrop, root-bark of spinystem aralia, honeycomb, polygonum cuspidatum, rhizome corydalis, stiff silkworm, yerbadetajo herb eclipate and semen benincasae. The invention has good cure effects on various tumors, in particular to malignancy, especially for cancer.

The invention discloses a Japan snakeroot strictosidine synthase gene, protein which is encoded by the Japan snakeroot strictosidine synthase gene and the use thereof. The Japan snakeroot strictosidine synthase gene which is provided by the invention has a nucleotide sequence or a homologous sequence which adds, replaces, inserts or losses one or a plurality of nucleotides or allele thereof and the nucleotide sequence which is derived from the Japan snakeroot strictosidine synthase gene, which are displayed in the SED ID No.1. The protein which is encoded by the gene has an amino acid sequence or the homologous sequence which adds, replaces, inserts or losses one or a plurality of amino acids, which is displayed in the SEQ ID No.2. The Japan snakeroot strictosidine synthase gene which is provided by the invention can increase the content of camptothecin which is an anticancer component in resource plants such as Japan snakeroot or camptotheca and the like through the genetic engineering technology and can be used in research and industrialization for increasing the content of the camptothecin through utilizing the transgenic technology, which is helpful for accelerating to solve the problem that the drug resources of the camptothecin are seriously scarce and has very good application prospect.

A new and distinct cultivar of Camptotheca lowreyana tree named `Katie`, characterized by its freely and vigorously branching; dense and full plant habit; small leaf size; lanceolate to elliptic leaf shape; leaves from both juvenile and mature trees with entire leaf margins; lower temperature and drought tolerance; and high yield of anti-cancer camptothecin in leaves.

The invention discloses oral cavity washing liquid. The oral cavity washing liquid is prepared from three raw materials including blumea balsamifera herb, scutellaria baicalensis leaves and camptotheca acuminate leaves in a weight ratio of (4-12):(6-15):(2-8). The invention further discloses a preparation method of the oral cavity washing liquid. The washing liquid provided by the invention has arelatively good inhibition effect on common oral pathogenic bacteria such as streptococcus mutans, staphylococcus and candida albicans, can be used for preventing and treating diseases such as oral ulcer, stomatitis, caries and oral lichen, and has the effects of cleaning the oral cavity, inhibiting bacteria, removing odor, refreshing breath and the like.