353results about How to "High proliferation rate" patented technology

The invention provides methods for predicting an increased risk or probability of developing endometriosis in a patient based upon the patient's KRAS variant status.

Toll Like Receptor 3 (TLR3) antagonists, polynucleotides encoding TLR3 antagonists or fragments thereof, and methods of making and using the foregoing are disclosed.

A biological tissue sheet which is expected as exerting a favorable therapeutic effect and a high safety in transplantation. The biological tissue sheet formed by (a) preparing in vivo-derived cells; (b) sowing the in vivo-derived cells on amniotic membrane; and (c) culturing and proliferating the in vivo-derived cells in the absence of any xenogeneic animal cells. As the cells of a biological origin, for example, cells originating in corneal epithelium, conjunctival epithelium, skin epidermis, hair follicle epithelium, oral mucosa, respiratory tract mucosa, or intestinal tract mucosa.

The invention relates to a ginseng adventitious root induced proliferation method, which comprises the following steps: cutting ginseng tissue culture seedlings into small tissue blocks and then inoculating into a solid induced culture medium for inducing to form adventitious roots, cutting the adventitious roots into small adventitious root blocks and then inoculating into a liquid proliferation culture medium for performing proliferation culture of the adventitious roots, wherein the solid induced culture medium and the liquid proliferation culture medium take 1 / 2 MS(-N) culture medium as basic culture mediums and contain 1-10mg / L indolebutyric acid. By utilizing the method provided by the invention, the quantity of the adventitious roots obtained through induction can be obviously increased, the adventitious roots obtained through induction are subjected to proliferation culture, and compared with the method for performing adventitious root induction by utilizing callus tissues, the proliferation rate of the adventitious roots is higher, and the proliferation effect is better.

The current invention provides monocytic cells transfected with chimeric antigen receptor (CAR) to selectively home to tumors and upon homing differentiate into dendritic cells capable of activating immunity which is inhibitory to said tumor. In one embodiment of the invention, monocytic cells are transfected with a construct encoding an antigen binding domain, a transcellular or structural domain, and an intracellular signaling domain. In one specific aspect of the invention, the antigen binding domain interacts with sufficient affinity to a tumor antigen, capable of triggering said intracellular domain to induce an activation signal to induce monocyte differentiation into DC.

The invention discloses a tissue culture propagation method for anoectochilus roxburghii, and relates to a tissue culture method for plants. The invention provides a tissue culture propagation method for anoectochilus roxburghii, through which the rapid propagation and growth of stem sections of anoectochilus roxburghii can be promoted quickly. The method comprises the following steps of: taking a stem section of anoectochilus roxburghii as an explant material, and carrying out disinfection on the explant; carrying out induction on adventitious buds of the stem section; cultivating tissue culture seedlings; and transplanting the tissue culture seedlings. Through selecting a middle stem section without containing terminal buds and nasociliary roots as a cultivating object, the survival rate is high, and the rate of adventitious bud multiplication is high. An operation of adding bananas into a culture medium is highly beneficial to promoting the growth of seedlings, and the net increase of leaves, the rooting number and the net increase of fresh weight are high. Through tissue culture experiments, the proliferation rate can be up to5.7 times.

The present invention is directed to bioactive botanical cosmetic compositions derived from membrane and cell serum fractions of plant cell juice. The present invention also relates to the methods for preparing these bioactive botanical cosmetic compositions and the uses of these compositions in various cosmetic formulations and as topical skin cosmetic applications.

The present invention relates to nucleic acid molecules and in particular to vectors, comprising at least one gene of interest, at least one scaffold / matrix attached region (S / MAR), at least one origin of replication, and at least one replication initiation factor, cells comprising these, processes for their propagation, and their use, in particular for the high level expression of proteins which can be used as medicaments.

Human adipose tissue-derived multipotent adult stem cells are provided, which are characterized by the ability to be maintained in an undifferentiated state for a long period of time by forming spheres and which have high proliferation rates. Also provided are methods for isolating and maintaining the adult stem cells, and methods for differentiating the multipotent adult stem cells into nerve cells, fat cells, cartilage cells, osteogenic cells, muscle cells, endothelial cells, hepatic cells and insulin-releasing pancreatic β-cells. Also provided are cellular therapeutic agents for treating osteoarthritis, osteoporosis, nerve disease, diabetes and for forming breast tissue, which contain differentiated cells or the adult stem cells.

The present invention is directed to bioactive botanical cosmetic compositions derived from membrane and cell serum fractions of plant cell juice. The present invention also relates to the methods for preparing these bioactive botanical cosmetic compositions and the uses of these compositions in various cosmetic formulations and as topical skin cosmetic applications.

The invention disclose a technique for the tissue culturing and breeding of the red-welcome cuckoo as well as the technique for domestication and replanting, which comprises the first generation culture of inducing the clump sprout by applying the germ-free seedling as explant, subculture for enrichment-culture, root-growing inducing for the clump sprout, water-planting for domestication before replanting the tissue culture sprout, the final replanting to flowerpot and getting the red-welcome cuckoo. The invention is suitable for the red-welcome cuckoo from different places, the k-factor of the clump sprout is high, the root is uniform, and the survival rate after the water-culture domestication is high and is suitable for the red-welcome cuckoo commercial production.

The present invention relates to isolated protein sequences that correspond to cell binding peptides, fragments, neo-structures and / or neo-epitopes of a normally occurring serum protein present in human tissue, wherein the peptide, fragment, neo-structure and / or neo-epitope has an immunoregulatory activity and is the result of either an enhanced proteolytic activity and / or conformational changes in a tissue, or a malignant tumour. In the present patent application, a common structure of several of these peptides, fragments, neo-structures and / or neo-epitopes, having immunoregulatory activity by binding to receptors on immune cells, has been identified. The present invention further also relates to monoclonal and / or polyclonal antibodies directed to a cell binding fragment of a normally occurring serum protein present in human tissue, as described above.

This invention relates to composition and methods of employing said composition for treating or preventing microbial associated infections and diseases. More particularly the present invention relates to bacterial proteins, peptides and amino acids which are by-products of bacteria, in particular Lactobacillus and more specifically Lactobacillus strains GR-1 and RC-14, in compositions that can treat and prevent microbial-associated infections and diseases, by altering, for example, down-regulating, virulence properties of pathogenic organisms.

The invention relates to a clonal tissue culture breeding method for a camphor tree and belongs to the technical field of forest tree tissue culture. The clonal tissue culture breeding method comprises the steps of explant material sterilization, bud induction, bud propagation, rooting induction, rooted-seedling hardening, and transplantation and management of tissue-cultured seedling, and particularly comprises the following steps of: removing the leaves of an annual shoot; cutting the shoot into stems which are respectively provided with 1-2 axillary buds; sterilizing the stems; carrying out induced culture by an inducing medium; carrying out enrichment culture and rooting induction on the obtained new bud; hardening and naturalizing the obtained rooted seedling; and finally transplanting the naturalized seedling into a matrix which is sterilized, and managing the transplanted seedling. Seedling raising by the clonal tissue culture breeding method for the camphor tree, disclosed by the invention, is not affected by natural factors such as season and weather, and besides, production cost is low, land resources are saved, and seedling raising efficiency is improved; meanwhile, with adoption of the clonal tissue culture breeding method disclosed by the invention, the phenomenon of browning can be effectively prevented, the effective reproduction rate is high, the rooted seedling is grown regularly, the culture period is short, the survival rate for the transplanting of the tissue-cultured seedling is high, and the seedling stage culture period is short; and the clonal tissue culture breeding method is significant.

The present invention is directed to bioactive botanical cosmetic compositions derived from membrane and cell serum fractions of plant cell juice. The present invention also relates to the methods for preparing these bioactive botanical cosmetic compositions and the uses of these compositions in various cosmetic formulations and as topical skin cosmetic applications.

The invention provides a high active induction culture method for lily bulbil which is characterized in that: through an induction culture of embryoid and tissue enrichment culture of embryoid tissue, a relative high rapid propagation velocity is obtained; then through an differentiation culture of bulb-lets and an intumescence culture of bulb-lets, transplanting bulb-lets of a circumferential diameter bigger than 3cm are obtained. The growth rate of the bulb-lets of the invention can reach more than one million grains per years which is ten times that of conventional methods. With a large number of small bulb-lets obtained, the ratio of bulb-lets with large specification and large grain size is increased. The invention optimizes the culture procedures of preferential lily seedball, shortens the propagation time of preferential lily seedball; furthermore, the field planting survival rate of bulb-lets is high and the production cost is low; the invention is suitable for the scale rapid propagation production of cut lily elite.

The invention discloses a growth promotion cultivation method of fritillary bulbs. The growth promotion cultivation method mainly comprises the steps of selection and disinfection of explants, material treatment, primary culture, subculture, proliferation of bulblets, rooting culture, and management of soil for acclimatization and transplanting. Different hormones are used for adjusting at different culture phases; a formula of a culture medium is sieved accurately to realize the good effect of vigorous growth of test-tube seedlings; an effective sterilization technology is combined to achieve the aims of improving the proliferation rate, reducing variation, increasing soil fertility and reducing plant diseases and insect pests. The culture medium is replaced properly in the proliferation process of the bulblets which are subjected to subculture so as to facilitate the rapid growth of the bulblets. By comparing different parts of flakes, a best flake reproduction material is the lower part of inner-layer flakes.

The invention relates to a clonal tissue culture breeding method of Liquidambar formosana Hance, belonging to the technical field of tree tissue culture breeding. The method comprises the following steps of: sterilization of explant materials, induction of sprouts, multiplication of sprouts, rooting induction, hardening of rooted seedlings, and transplanting and management of tissue culture seedlings. Specifically, the method comprises the steps of: removing leaves of a one-year-old shoot, cutting the shoot into sections with 1-2 axillary buds respectively, sterilizing the sections, conducting induced culture to the sections by using induced culture media, conducting multiplication culture and rooting culture to new sprouts, hardening and domesticating the cultured rooted seedlings, finally transplanting the domesticated seedlings on sterilized media, and managing the transplanted seedlings. By adopting the method, the breeding is not limited by seasons, the production cost is low and the land resources are saved; and the situation of browning can be effectively avoided, the effective multiplication rate is high, the rooted seedlings grow tidily, the culture period is short, the survival rate of the transplanted tissue culture seedlings is high, the seedling cultivation period is short, the clonal breeding of the Liquidambar formosana Hance can be pushed forward and the method plays an important role in large-scale propagation and popularization of improved varieties.

The invention relates to the technical field of medical artificial bone transplantation materials. At present, bone transplantation is widely distributed in multiple fields such as orthomorphia, oral cavity, craniofacial region and the like. But all preparation methods of artificial bone transplantation materials have the problems that the manufacture period is long, the yield is low, and prepared artificial bone transplantation materials do not have precise anatomical morphology same to that of natural bone, and the like. The invention aims at providing a nanometer artificial bone scaffold possessing good bioactivity and excellent mechanical properties and a structure similar to that of natural bone. A selective laser sintering technology is employed, poly-epsilon-caprolactone and hydroxylapatite are taken as raw materials, and layer-by-layer sintering is performed according to a designed model and set parameters, and finally the artificial bone scaffold with the structure similar to that of natural bone is obtained. The artificial bone scaffold has outstanding biocompatibility, cells have relatively high propagation rate on the artificial bone scaffold, and the scaffold keeps good biomechanical properties.

The invention provides a method for rapidly breeding seedlings by using bletilla striata tubers. According to the method, rapid breeding is realized by using bletilla striata tubers, and the technical route of synchronization of bud increase and rooting is adopted, complete plants can be formed after about 50 days, and a large number of complete plants can be obtained within 4-6 months, so that large-scale industrial seedling breeding of bletilla striata is realized. The method for rapidly breeding seedlings by using bletilla striata tubers is simple, and the seedling breeding cost is reduced greatly. By adopting the method, quick budding and high reproduction rate are realized, the method is simple, tissue culture equipment is not required to be added, the production cost is low, batch production is realized and the application value is high.

The invention provides a tissue engineering meniscus scaffold and a preparation method thereof. The preparation method comprises the following steps: placing a polycaprolactone material into a nozzle of a fused deposition modeling three-dimensional printer to be heated to 120-140 DEG C, and preparing for printing at the air pressure of 600-1000 kPa; setting the printing speed of the fused deposition modeling three-dimensional printer to be 0.6-0.75 mm / s, the fiber diameter to be 300-320 [mu]m and the fiber interval to be 200-300 [mu]m and printing a tissue engineering meniscus scaffold; using cobalt-60 for irradiation and sterilization treatment. As the fused deposition modeling method is adopted to print the tissue engineering meniscus scaffold with an appropriate pore structure, an appropriate fiber diameter and appropriate biomechanical strength, the survival rate and the reproduction rate of inoculated cells and autologous in-growth cells of implanted regions are favorably increased, the fibrous cartilage differentiation rate can be increased, and newly generated meniscus tissues have favorable functions.

The invention relates to a method for improving tissue culture reproductive speed of Alpinia zerumbet, which relates to the tissue culture technology of Alpinia zerumbet, and comprises the following steps that: A. seed collection is proceeded after 7 to 8 continuous sunny days, clumped Alpinia zerumbet plants which are used as seed are dug out from the soil 2 to 3 days before the seed production, soil is removed, and the Alpinia zerumbet plants are hung upside down to be dried for 2 to 3 days at a cool well-ventilated indoor place; B. lateral buds on tubers or spouts are selected as explant materials; C. adventitious buds are inducted to produce; D. adventitious buds are bred and cultured; E. adventitious buds are rooted to culture; and F. seedlings are transplanted. The rapid formation of adventitious buds is promoted through high temperature and high-concentration hormone, so the induction efficiency is improved, and the culture time is shortened; the formation of variegated leaf solid buds can be realized by changing the culture medium formula, reducing the culture temperature and increasing the light density, whitening variation can be reduced, the rapid breeding rate and seedling formation rate can be effectively improved, the factory-oriented production progress of the tissue cultured seedling can be accelerated, and the production cost can be reduced.

The invention provides a method for inducing adventitious buds by adopting Lycoris radiate rachis as explant in order to obtain regenerated plants. The method comprises the following steps: selection and disinfection of the explant, induction of the adventitious buds, subculture and propagation, root induction, and transplantation of tissue culture plants. The method allows the induction germination rate to reach 96%, 4.9 times or above adventitious buds to be proliferated from a per plant and the rooting rate to reach 94%, and can effectively solve the problem of in vitro rapid propagation of Lycoris radiate.

A tissue culturing method for high-quality papaya sprout features that the adult lateral bud of the disease-resistant female papaya tree is chosen as explant, and the conditions for tissue culture and rooting culture are disclosed for higher effect.

The present invention relates to a culture medium for culturing a mesenchymal stem cell, and more specifically, to a culture medium composition for culturing a mesenchymal stem cell, comprising a basic culture medium, L-ascorbic acid 2-phosphate, fetal bovine serum, basic fibroblast growth factor (b-FGF), non-essential amino acids (NEAA), insulin, N-acetyl-L-cysteine, calcium chloride and hydrocortisone,and a method for culturing a mesenchymal stem cell by using the same. According to the present invention, it is possible to obtain the number of mesenchymal stem cells necessary for stem cell treatment within a short time and the differentiation of mesenchymal stem cells is improved, and is useful for the treatment of cells using a stem cell.

The invention relates to a selenium-rich microbial preparation prepared by fermentation of animal serum. The selenium-rich microbial preparation comprises the following raw materials in parts by weight: 8-15 parts of sodium selenite, 50-65 parts of animal blood hydrolysate, 5-15 parts of composite trace elements, 8-20 parts of potassium dihydrogen phosphate, 10-25 parts of urea, 8-20 parts of peptone, 4-10 parts of yeast powder, 8-20 parts of NaCl, 8-15 parts of NaOH, 4-10 parts of NaNO3, 10-25 parts of NaCl, 4-10 parts of NH4Cl, 8-20 parts of K2HPO4, 4-10 parts of beef extract, 10-25 parts of glucose, 10-25 parts of starch, 8-20 parts of calcium carbonate, 10-25 parts of amino acid, 2-6 parts of potassium nitrate, 2-6 parts of ferrous sulfate, 10-25 parts of magnesium sulfate, 25-40 parts of trisodium citrate, 10-25 parts of an biochemical enzyme and 800-848 parts of a mixed bacterial solution. According to the preparation method of the selenium-rich microbial preparation provided by the invention, the fermentation time is short, and the preservation period of the microbial agent can be prolonged to 2 years by increasing the proliferation speed of microbes in the fermentation process.