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Tissue culture propagation method for anoectochilus roxburghii

A clematis and tissue culture technology, applied in the field of plant tissue culture, can solve problems such as deterioration of natural conditions, human behavior damage, slow growth rate, etc., and achieve high proliferation rate of adventitious buds, high number of roots, and net increase in fresh weight high volume effect

Active Publication Date: 2013-02-06
厦门加晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, clematis has a low reproductive rate and a very slow growth rate under natural conditions, coupled with the deterioration of natural conditions and the destruction of human behavior, it has caused it to be on the verge of extinction, causing the supply of clematis to be in short supply.

Method used

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  • Tissue culture propagation method for anoectochilus roxburghii
  • Tissue culture propagation method for anoectochilus roxburghii

Examples

Experimental program
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Embodiment 1

[0027] The wild clematis clematis stems after removing the leaves of clematis were selected as explants, and the tissue culture was carried out in the following four steps in this embodiment.

[0028] 1) Take the stem section of wild clematis as explant material, and sterilize the explant.

[0029] 2) Induction of adventitious buds in stem segments.

[0030] 3) Cultivation of tissue culture seedlings.

[0031] 4) Transplanting tissue culture seedlings.

[0032] Equipment and items that need to be prepared during the explant disinfection process: sterilized beakers, glass rods, 70% alcohol, 0.1% HgCl solution, sterile water, stopwatch, etc. The specific disinfection process is:

[0033] In the first step, the explants are placed in flowing tap water and rinsed for about 2 hours;

[0034] The second step is to infiltrate and sterilize the rinsed explants. The sterilization process was completed in a horizontal ultra-clean workbench, and the explants were placed in a sterili...

Embodiment 2

[0048] 1) Take the stem section of clematis as the explant material, and sterilize the explant; the clematis is preferably wild clematis; the explant is the wild clematis after removing the leaves of clematis Lotus stem section; the concrete method of described explant disinfection can be:

[0049] (1) Washing the explants in running water; the washing time may be 1 hour.

[0050] (2) Infiltrate and sterilize the washed explants; the sterilization can be completed in a horizontal ultra-clean workbench, and the explants are placed in a sterilized container and soaked in alcohol, and the soaking time can be 20s .

[0051] (3) Put the explants after infiltration sterilization in step (2) into HgCl solution for sterilization, the mass fraction of the HgCl solution may be 0.1%, and the sterilization time may be 10 min.

[0052] (4) Rinse the explants treated in step (3) with sterile water, the conditions for the rinsing may be 8 times, and the time for each rinsing may be 1 min. ...

Embodiment 3

[0057] 1) Take the stem section of clematis as the explant material, and sterilize the explant; the clematis is preferably wild clematis; the explant is the wild clematis after removing the leaves of clematis Lotus stem section; the concrete method of described explant disinfection can be:

[0058] (1) Washing the explants in running water; the washing time may be 2 hours.

[0059] (2) Infiltrate and sterilize the washed explants; the sterilization can be completed in a horizontal ultra-clean workbench, and the explants are placed in a sterilized container and soaked in alcohol, and the soaking time can be 10s .

[0060] (3) Put the explants after infiltration sterilization in step (2) into HgCl solution for sterilization, the mass fraction of the HgCl solution may be 0.1%, and the sterilization time may be 20 minutes.

[0061] (4) Rinse the explants treated in step (3) with sterile water, the conditions for the rinsing may be 5 times, and the time for each rinsing may be 5 ...

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Abstract

The invention discloses a tissue culture propagation method for anoectochilus roxburghii, and relates to a tissue culture method for plants. The invention provides a tissue culture propagation method for anoectochilus roxburghii, through which the rapid propagation and growth of stem sections of anoectochilus roxburghii can be promoted quickly. The method comprises the following steps of: taking a stem section of anoectochilus roxburghii as an explant material, and carrying out disinfection on the explant; carrying out induction on adventitious buds of the stem section; cultivating tissue culture seedlings; and transplanting the tissue culture seedlings. Through selecting a middle stem section without containing terminal buds and nasociliary roots as a cultivating object, the survival rate is high, and the rate of adventitious bud multiplication is high. An operation of adding bananas into a culture medium is highly beneficial to promoting the growth of seedlings, and the net increase of leaves, the rooting number and the net increase of fresh weight are high. Through tissue culture experiments, the proliferation rate can be up to5.7 times.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture rapid propagation method of Aurea clematis. Background technique [0002] Because clematis has significant medicinal effects, such as clearing heat, cooling blood, lowering blood pressure, antibacterial, and anticancer, the medicinal value of clematis has been recognized by the world and is widely used. However, clematis has a low reproductive rate and a very slow growth rate under natural conditions. In addition, the deterioration of natural conditions and the destruction of human behavior have caused it to be on the verge of extinction, causing the supply of clematis to fall short of demand. [0003] In order to meet the needs of the market, artificial propagation and cultivation techniques of Clematis clematis have been extensively studied and applied. In seed propagation, cutting propagation and tissue culture propagation, tissue culture propagation is the mo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 方金镇郑志红方加强
Owner 厦门加晟生物科技有限公司
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