Growth promotion cultivation method of fritillary bulbs

A cultivation method, the technology of flat fritillary, applied in the breeding field of flat fritillary, to achieve the effects of increasing economic benefits, improving quality and survival rate, and shortening the breeding cycle

Inactive Publication Date: 2013-12-18
SUZHOU RENCHENG BIO SCI & TECH
View PDF7 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the existing problems, the present invention provides a method that can quickly and effectively utilize existing resources to propagate flat fritillary

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A growth-promoting cultivation method of Fritillaria fritillaria, comprising the following steps:

[0027] (1) Selection and disinfection of explants: firstly, select fresh Fritillaria fritillaria that grew vigorously in that year without mildew spots, cut off the roots and damaged scales, rinse with running water for 30 minutes, soak in clean water for 15 minutes, and then use 50% Soak in 500-fold solution of carbendazim for 20 minutes or soak in 500-fold solution of 50% thiram powder for 10 minutes, then peel off the scales of the bulbs, irradiate them with ultraviolet light for 10 minutes, transfer them to an ultra-clean workbench, and then disinfect them with 75% alcohol for 50 minutes. s, rinsed twice with sterile water, then disinfected with 6% sodium hypochlorite solution for 10 min, and rinsed three times with sterile water. Finally, they were disinfected with 0.2% mercuric chloride solution for 10 min and rinsed with sterile water 4 times.

[0028] (2) Materia...

Embodiment 2

[0035] Select the lower part of the outer scales for the material in the material treatment process of step (2), and the other conditions are the same as in Example 1. As a result, the callus formed after 27 days in the primary culture, and differentiated after 9 days in the subculture. Green dots, in the process of the proliferation of bulblets, after 12 days of cultivation, cluster bulblets can be differentiated, and in the process of rooting culture, the bulblets grow to 2.5cm in 11 days, and the root system is relatively developed.

Embodiment 3

[0037] Select the lower part of the outer scales for the material in the material treatment process of step (2), and the other conditions are the same as in Example 1. As a result, the callus formed after 27 days in the primary culture, and differentiated after 8 days in the subculture. Green dots, in the propagation process of bulblets, after 12 days of cultivation, cluster bulblets can be differentiated, and in the process of rooting culture, the bulblets grow to 2.5cm in 10 days, and the root system is relatively developed.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a growth promotion cultivation method of fritillary bulbs. The growth promotion cultivation method mainly comprises the steps of selection and disinfection of explants, material treatment, primary culture, subculture, proliferation of bulblets, rooting culture, and management of soil for acclimatization and transplanting. Different hormones are used for adjusting at different culture phases; a formula of a culture medium is sieved accurately to realize the good effect of vigorous growth of test-tube seedlings; an effective sterilization technology is combined to achieve the aims of improving the proliferation rate, reducing variation, increasing soil fertility and reducing plant diseases and insect pests. The culture medium is replaced properly in the proliferation process of the bulblets which are subjected to subculture so as to facilitate the rapid growth of the bulblets. By comparing different parts of flakes, a best flake reproduction material is the lower part of inner-layer flakes.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a propagation method of Fritillaria fritillata. Background technique [0002] Flat Fritillaria, also known as Pingbei, Beibei, and Fritillaria, is a perennial herb of the family Liliaceae, up to 1m high. The dried bulb of Fritillaria is a commonly used Chinese medicinal material in my country. It has the functions of clearing away heat and moistening the lungs, resolving phlegm and relieving cough, and dispelling stagnation. treat. In recent years, due to over-excavation, resources have been decreasing year by year, the supply of commodities is in short supply, and prices have continued to rise. [0003] At present, the research on tissue culture of Fritillaria has reached a certain scale, but in view of the technical problems, there are always some problems, and there has been some research on the rapid reproduction technology of Fritillaria, but the demand for Fritillaria is increasi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 吴志刚
Owner SUZHOU RENCHENG BIO SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap