Method for breeding fritillary bulb by tissue culture technique

A technology of tissue culture and flat fritillaria, applied in horticultural methods, botanical equipment and methods, applications, etc., to achieve the effects of increasing economic benefits, efficiently inducing explant differentiation and proliferation, and vigorous growth

Inactive Publication Date: 2013-08-14
TAICANG JINZHU AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the existing problems, the present invention provides a method that can quickly and effectively utilize existing resources to propagate flat fritillary

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for propagating flat fritillary by tissue culture technology, characterized in that it comprises the following steps:

[0023] (1) Selection and disinfection of explants: firstly, select fresh Fritillaria fritillaria that grew vigorously in that year without mildew spots, cut off the roots and damaged scales, rinse with running water for 30 minutes, soak in clean water for 15 minutes, and then use 50% Soak in 500-fold solution of carbendazim for 20 minutes or soak in 500-fold solution of 50% thiram powder for 10 minutes, then peel off the scales of the bulbs, irradiate them with ultraviolet light for 10 minutes, transfer them to an ultra-clean workbench, and then disinfect them with 75% alcohol for 50 minutes. s, rinsed twice with sterile water, then disinfected with 6% sodium hypochlorite solution for 10 min, and rinsed three times with sterile water. Finally, they were disinfected with 0.2% mercuric chloride solution for 10 min and rinsed with sterile water 4...

Embodiment 2

[0031] Select the lower part of the outer scales for the material in the material treatment process of step (2), and the other conditions are the same as in Example 1. As a result, the callus formed after 27 days in the primary culture, and differentiated after 9 days in the subculture. Green dots, in the process of the proliferation of bulblets, after 12 days of cultivation, cluster bulblets can be differentiated, and in the process of rooting culture, the bulblets grow to 2.5cm in 11 days, and the root system is relatively developed.

Embodiment 3

[0033] Select the lower part of the outer scales for the material in the material treatment process of step (2), and the other conditions are the same as in Example 1. As a result, the callus formed after 27 days in the primary culture, and differentiated after 8 days in the subculture. Green dots, in the propagation process of bulblets, after 12 days of cultivation, cluster bulblets can be differentiated, and in the process of rooting culture, the bulblets grow to 2.5cm in 10 days, and the root system is relatively developed.

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PUM

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Abstract

The invention discloses a method for breeding fritillary bulb by a tissue culture technique. The method mainly comprises the following steps: selection and disinfection of explants, material treatment, primary culture, subculture, proliferation of bulblet, rooting culture, acclimatization and transplant and the like. In different culture stages, different hormones are used for adjusting, and the formula of culture medium is accurately screened to realize the excellent effect of vigorous growth of test-tube plantlet. The purpose of improving the proliferation rate and reducing mutation is realized by combining effective sterilization technologies. The culture medium is appropriately changed in the bulblet subjected to proliferation process to facilitate quick growth of the bulblet. The optimal selection of the scale breeding material is the lower part of the inner scale by comparing different parts of the scale.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a propagation method of Fritillaria fritillata. Background technique [0002] Flat Fritillaria, also known as Pingbei, Beibei, and Fritillaria, is a perennial herb of the family Liliaceae, up to 1m high. The dried bulb of Fritillaria is a commonly used Chinese medicinal material in my country. It has the functions of clearing away heat and moistening the lungs, resolving phlegm and relieving cough, and dispelling stagnation. treat. In recent years, due to over-excavation, resources have been decreasing year by year, the supply of commodities is in short supply, and prices have continued to rise. [0003] At present, the research on tissue culture of Fritillaria has reached a certain scale, but in view of the technical problems, there are always some problems, and there has been some research on the rapid reproduction technology of Fritillaria, but the demand for Fritillaria is increasi...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 陈勇明
Owner TAICANG JINZHU AGRI DEV
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