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Ginseng adventitious root induced proliferation method

A technology of ginseng and induction medium, which is applied in the field of plant tissue culture, can solve the problems of long experimental period, etc., and achieve the effect of increasing the number of adventitious roots, good proliferation effect, and shortening the time

Active Publication Date: 2015-04-01
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the above method is that the callus needs to be induced first, resulting in a long experimental period.

Method used

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  • Ginseng adventitious root induced proliferation method
  • Ginseng adventitious root induced proliferation method
  • Ginseng adventitious root induced proliferation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This example is used to illustrate the use of ginseng tissue culture seedling roots to induce adventitious roots.

[0052] Get the roots of ginseng tissue culture seedlings, cut them into small sections of about 5 mm × 5 mm × 3 mm with a sterilized knife, and transfer them to the solid induction medium containing 1 / 2MS(-N)+2mg / L IBA (in the solid induction medium Contain 2% sucrose, 7g / L agar, pH value is 5.8), induce the production of adventitious roots under dark conditions at 24±2°C. Each dish was inoculated with 30 explants, subcultured every three weeks during the induction culture process, and the adventitious root induction rate and the number of adventitious roots produced by each explant were counted. The experiment was repeated 3 times, and the average value was taken. The results are listed in Table 1.

[0053] The result shows that after three weeks of cultivation, all roots can induce adventitious roots, and only one adventitious root is induced on each roo...

Embodiment 2

[0055] This example is used to illustrate the proliferation and culture of adventitious roots induced by ginseng tissue culture seedling roots.

[0056] The adventitious roots induced by the roots of ginseng tissue culture seedlings obtained after six weeks of induction in Example 1 were taken out, cut into small pieces of 1 cm size, and inserted into small pieces containing 100ml 1 / 2MS(-N)+2mg / L IBA +20g / L sucrose in a culture bottle of liquid medium, placed in a shaker at 24±2°C under dark conditions and slowly oscillating (60rpm) to carry out proliferation of adventitious roots. Subculture once every three weeks, and count the fresh weight of adventitious roots, the experiment was repeated 3 times, and the average value was taken. The cultured adventitious roots were taken out, the sterilized filter paper absorbed the culture solution on the surface, and placed on a balance for weighing, which was the fresh weight. Calculate the proliferation rate of adventitious roots, th...

Embodiment 3

[0058] This example is used to illustrate the proliferation and culture of adventitious roots induced by ginseng tissue culture seedling roots.

[0059] According to the same method as in Example 2, the adventitious roots induced by the roots of ginseng tissue culture seedlings were proliferated and cultured. The only difference is that the liquid proliferation medium used is 1 / 2MS(-N) liquid proliferation medium containing 5mg / L IBA and 20g / L sucrose. Calculate the proliferation rate of adventitious roots, the results show that ( figure 2 A): After three weeks of proliferation culture, the proliferation rate was 24.4%; after six weeks of proliferation culture, the proliferation rate was 32.27%, and after nine weeks of culture, the proliferation rate was 22.25%.

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Abstract

The invention relates to a ginseng adventitious root induced proliferation method, which comprises the following steps: cutting ginseng tissue culture seedlings into small tissue blocks and then inoculating into a solid induced culture medium for inducing to form adventitious roots, cutting the adventitious roots into small adventitious root blocks and then inoculating into a liquid proliferation culture medium for performing proliferation culture of the adventitious roots, wherein the solid induced culture medium and the liquid proliferation culture medium take 1 / 2 MS(-N) culture medium as basic culture mediums and contain 1-10mg / L indolebutyric acid. By utilizing the method provided by the invention, the quantity of the adventitious roots obtained through induction can be obviously increased, the adventitious roots obtained through induction are subjected to proliferation culture, and compared with the method for performing adventitious root induction by utilizing callus tissues, the proliferation rate of the adventitious roots is higher, and the proliferation effect is better.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing proliferation of ginseng adventitious roots. Background technique [0002] Ginseng (Panax ginseng C.A. Meyer) is a perennial dicotyledonous plant of the genus Panax of Araliaceae. It is an ancient and precious Chinese medicinal material, which has various functions such as regulating human immunity, anti-cancer and anti-diabetes. Ginsenoside is its main active substance. In recent years, the demand for ginseng has grown rapidly at home and abroad. However, due to long-term over-harvesting, the wild resources of ginseng have been basically exhausted, and the ginseng cultivation cycle is long, which is easily affected by climate, pests and diseases and cultivation conditions, making it difficult to meet the growing market demand. Ginseng tissue culture technology has a short cycle, is not limited by seasons, and is easy to carry out large-scale ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 卢善发王梅珍
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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