48 results about "Magnolia grandiflora" patented technology

A grafting cultivation method of magnolia denudata and magnolia grandiflora relates to the technical field of sapling cultivation. The method comprises breeding two-year or more lignified magnolia denudata branches into scion seedlings in the first year; after the scion seedlings germinates, selecting the scion seedlings which have stocky stems and roots as scions; taking magnolia grandiflora as stocks for grafting through routine methods in March and April in the second year; transplanting the grafted plants in fixed pits to in north China, and after the plants grow two to three years, performing plant transplantation. The grafting cultivation method of the magnolia denudata and the magnolia grandiflora is simple, shortens the defoliation cycle of the magnolia denudata and improves the survival rate of the magnolia denudata; the magnolia denudate cultivated through the method is good in disease-resistant and drought-resistant capacities and particularly applicable to urban afforestation.

The invention provides a biological plant source miticide prepared from urban greening wastes, and preparation and application of the biological plant source miticide. The biological plant source miticide is composed of 40%-80% of bulk drugs and 20%-60% of conventional auxiliary materials. The bulk drugs comprise the following components in percenrage by weight: 2%-20% of ligustrum lucidum extract, 5%-30% of ailanthus altissima extract, 5%-30% of liriodendron chinensis extract, 5%-10% of camptotheca acuminate extract, 5%-25% of magnolia grandiflora linn extract, 5%-30% of koelreuteria paniculata extract, 5%-20% of cinnamomum camphora extract, 5%-15% of sapium sebiferum extract and 4%-10% of berberis thunbergii DC. extract. The plant extracts are all obtained by drying, powdering, leaching and filtering branches, stems, leaves, flowers and fruits or mixtures of these parts, which are cut from plants, or shed and wasted naturally or wasted in the process of processing other products. The biological plant source miticide is efficient, free from residuals, low in production cost, high in combination property as well as economical, practical and environment-friendly.

The invention relates to a rapid culture method for magnolia grandiflora and belongs to the technical field of plant grafting. The method comprises the following steps: carrying out rootstock culture: culturing Magnolia denudata as rootstock at the last ten days of March, and carrying out disinfecting and fertilizing treatment on culture seedling beds; carrying out grafting preparation: carrying out grafting at the second or third 10 days of March; carrying out graft selecting: selecting annual magnolia grandiflora branches as grafts; carrying out a grafting process: smearing rootstock incisions and graft faces with a disinfecting and growth-promoting solution during grafting; carrying out grafting management: shading grafted seedlings with shading nets after grafting is completed, removing binding films when the length of each grafted graft bud reaches 20cm, and carrying out top application with a compound fertilizer; and carrying out transplanting management: transplanting the grafted seedlings out of gardens after the grafted seedlings are cultured for 2 years. The rapid culture method for the magnolia grandiflora, provided by the invention, has the advantages that the survival rate of the grafted seedlings is high, and the growth speed is high.

The invention relates to an extraction and preparation method of parthenolide, and particulary relates to an extraction method of parthenolide from Magnolia grandiflora root bark. The extraction and preparation method comprises: crushing Magnolia grandiflora root bark as a raw material, extracting with an appropriate polar organic solvent to obtain total lactones, separating the total lactones by column chromatography, collecting the required ingredients, and then crystallizing the collected ingredients with an appropriate solvent to obtain parthenolide. The extraction method of parthenolide from Magnolia grandiflora root bark provided by the invention is suitable for industrial production.

The invention discloses an efficient magnolia grandiflora cultivation method. The method includes the following steps of land selection and preparation, field planting, transplanting, pruning and later period management. By the efficient magnolia grandiflora cultivation method, growth rate of the magnolia grandiflora is increased greatly, survival rate is increased, growth density of magnolia grandiflora plantlets is increased for providing larger growth space for the magnolia grandiflora, applied fertilizer is matched for providing nutritional guarantee to the magnolia grandiflora, pruning and later period management are matched to allow the magnolia grandiflora to obtain enough sunlight and enable full-grown magnolia grandiflora to be more attractive in appearance, and ornamental value and economic value of the magnolia grandiflora are heightened.

The invention discloses an efficient magnolia grandiflora culture method. According to the method, the close planting enhancement is carried out during the seedling culture, when seedlings grow to a state that the trunk diameter is greater than 5cm, the local seedlings are subjected to additional fertilization, short cutting measures are taken for seedlings which are not subjected to additional fertilization, after the trunk diameter of the seedlings subjected to the additional fertilization reaches 10 to 12cm, the seedlings subjected to the additional fertilization are transplanted and are supplied to the market, and the rest seedlings are subjected to additional fertilization. The efficient magnolia grandiflora culture method provided by the invention solves the problems of slow benefit obtaining and low integral benefits in the traditional magnolia grandiflora culture technology. The cultured seedlings are sturdy, the crown shape and the trunk shape are very good, the pest and disease damage is little, the seedling root system is well developed, the seedling yield is high, the transplanting survival rate of greening seedlings is high, and the adaptability is high. The magnolia grandiflora culture mode has the advantages that on the basis of sufficiently utilizing the photosynthesis theoretical knowledge of trees and the forest ecological structure theory, the seedlings are creatively configured, various culture measures are flexibly utilized, the operation is simple, convenient and feasible, in addition, the cost is low, and the popularization and the application are easy.

The present invention relates to a lobule dwarf type magnolia grandiflora tender stem segment isolated culture method, and belongs to the technical field of plant tissue culture. The method comprises: 1) selecting and pre-treating a material to be inoculated, 2) sterilizing an explant, 3) establishing a sterile system, 4) carrying out induction and proliferation culture on clustered shoots, 5) carrying out strong seedling and rooting culture, and 6) carrying out seedling exercising transplantation on test-tube seedlings. The lobule dwarf type magnolia grandiflora tender stem segment isolated culture method has the following beneficial effects that: the clustered shoot induction rate achieves more than 90%, the rooting rate after the strong seedling and rooting culture achieves more than 96%, the survival rate can achieve more than 96%, advantages of rapid reproduction, high reproduction rate, virus removal, purification rejuvenation and the like are provided, the defects of low reproduction coefficient, long period, seasonal restriction and the like in the prior art are overcome, and the high quality seedling can be produced within a short time in a factory manner so as to meet requirements of rural and urban greening. In addition, the one stem segment is adopted as the sample, tens of thousands of the seedlings can be rapidly propagated within a year in the factory manner, and the quality is excellent.

The invention discloses a plantation method capable of preventing magnolia grandiflora from lodging, which comprises the following steps of: field selection; planting; field management; digging a drainage trench in flat magnolia grandiflora fields or farmlands every 5-6m, wherein the drainage trench is 30-40cm in depth and 20-25cm in width. The method disclosed by the invention is a new method firstly adopted in the production for preventing the magnolia grandiflora from lodging, and makes up the blank of a method for preventing the magnolia grandiflora from lodging in the prior art. After being adopted, the method for preventing the magnolia grandiflora from lodging can obviously reduce wind-fall rare of the magnolia grandiflora in the flat field or farmland in a large scale by over 95%; the loss of large seedlings can be obviously reduced; the economic loss of the production operators of seedlings can be greatly relieved; in addition, the seedlings can grow strongly and compared with the prior art, the growth indicator of the seedlings is over 15%; the cultured seedlings are thicker and more strong, the degree of lignification is high, plant diseases and insects pests are very few, the seedling outplanting is high, the planting survival rate of the seedlings provided for greening is high, and the adaptability is strong. The method disclosed by the invention is simple and feasible, low in cost, and easy in population and application.

The invention mainly relates to the field of planting, and discloses a storage method of magnolia grandiflora seeds. The method comprises the steps: picking, drying, freezing, immersing, ultrasonically stirring the seeds, drying, and storing. The method is simple and easy in operation, the magnolia grandiflora seeds can be stored for 3 years, the germination rate of the seeds after being stored for 3 years is 73 percent, the germination time is 12 to 13 days, the seeds are saved, and the economic income can be increased by 12.6 percent; the seeds are dried after being picked, so that the moisture content is reduced, and the absorption of a treatment solution in later treatment can be accelerated; low-temperature freezing is carried out after an alcohol solution is added, so that icing is prevented, the stress resistance of germs is stimulated at low temperature, the activity of the germs is kept, and the storage period is prolonged; a seed-dressing agent is a pure plant extraction agent and is safe and non-toxic to a human body and the environment, the seeds are ultrasonically stirred in the seed-dressing agent, the immersion of the seed-dressing agent on the seeds is facilitated, nutrition is provided, an antibacterial and insecticidal effect is achieved, and the storage period is prolonged; after the seeds are baked and dried, the activity of the germs in the seeds is kept, the high-temperature stress resistance of the germs is stimulated, and the storage period is prolonged.

The present invention relates to a method useful for topical compositions and the compositions. The topical compositions comprise centella asiatica extract, magnolia grandiflora bark extract or magnolia officinalis bark extract, hespyridine methyl chalcone, pea extract, citrus grandis peel extract, magnolia biondii flower extract, dipeptide-2, palmitoyl tetrapeptide-7, tripeptide-1, and optionally centella asiatica meristematic cell culture. The invention also provides methods thereof for application and production.

The invention mainly relates to the technical field of planting and discloses a treatment method for increasing the germination rate of magnolia grandiflora seeds. The treatment method comprises the following steps: soaking seeds, freezing, mixing seeds, performing germination acceleration, and sowing the seeds. The method is simple, the magnolia grandiflora seed treatment time is shortened to 6-7days, buds can grow out within 5-6 days after sowing, the germination rate is up to 97.6%, the seedling breeding efficiency of the magnolia grandiflora seed is remarkably improved, and a great deal of seedling breeding cost is reduced; the magnolia grandiflora seeds are soaked in a composite enzyme solution, so that seed coat strucruers of the seeds are damaged, seed coat softening can be accelerated, seed dormancy can be primarily broken, and seed germination can be accelerated; after being soaked, the seeds are frozen for a short time in a refrigerator, then seed dormancy is further broken,the germination capability of the seeds is improved, the stress resistance of radicles and germs is improved, and the germination rate of the seeds is increased.

The invention discloses a magnolia grandiflora seed germination accelerating method. The method includes following steps: (1), putting picked seeds in a sodium hypochlorite solution for soaking and disinfecting for 30-40min, and using water to flush sodium hypochlorite left on the surfaces; (2), putting disinfected seeds in a pig intestine, adding seed activation liquid into the pig intestine, and using cotton ropes to seal two ends of the pig intestine; (3), sowing the activated seeds on a seedbed, and using diluted nutritional liquid to sufficiently water in one time. The magnolia grandiflora seed germination accelerating method has remarkable effect, germination rate of the seeds can be increased by about 15%, hereditary stability of the seeds is high, seedlings grow fast and are high in stress resistance after the seeds germinate, and quality of magnolia grandiflora is improved greatly.

The invention discloses a cultivation method of magnolia grandiflora. Before seeds are stored, kerosene is mixed into the seeds or the seeds are subjected to zinc phosphide treatment, so that damage of rats and insects to the seeds can be reduced; before seedling culture, a seedbed is subjected to disinfection treatment, so that the survival rate after seedling culture can be improved; by means of components and proportioning of nutrient soil, stem diameter of the magnolia grandiflora can be thickened, the number of newly-grown branches is increased, the number of dead branches is reduced, and the resistance of a plant body is improved; plant ash and carbofuran are spread on a long restbalk, so that underground insects can be controlled and seedling lifting is facilitated. The magnolia grandiflora cultivated by adopting the cultivation method has the advantages of good stress resistance and excellent growth, as well as has huge market potential and a broad prospect.

The invention relates to a high-survival rate magnolia grandiflora grafting propagation method. The high-survival rate magnolia grandiflora grafting propagation method specifically comprises the following steps of (1) scissoring and storage of cions in advance, (2) second cutting of cuts of stocks and (3) grafting propagation. Experiments show that the high-survival rate magnolia grandiflora grafting propagation method can improve the grafting survival rate of magnolia grandiflora to more than 93.6% from 82.4% in the prior art and accordingly achieve significant effects.

The invention belongs to the technical field of vegetable insect prevention and particularly relates to a vegetable insecticide and a preparation method thereof. The vegetable insecticide is prepared from raw materials in parts by weight as follows: 10-15 parts of camellia assamica meal, 10-15 parts of phoebe bournei, 10-15 parts of michelia foveolata, 10-15 parts of magnolia grandiflora, 6-10 parts of sodium nitroprusside, 4-8 parts of a penetrant and 4-8 parts of a modified starch solution. The vegetable insecticide is prepared from natural plants and so has the advantages of being healthy, non-toxic and pollution-free, and the vegetable insecticide has a good insecticidal effect, has the effect of promoting chlorophyll synthesis and has great popularization value.

The invention discloses a Magnolia Grandiflora Linn gardening form directive culturing method, belongs to the arbor form cultivation method field, and aims to provide the Magnolia Grandiflora Linn gardening form directive culturing method capable of improving grafting success rate on the scion and stock grafting portion; the method comprises the following steps: nursery selection, stock cultivation, stock growth, nursery stock grafting, seedling trimming, lateral bud processing, branch selection, modeling trimming, and repeatedly trimming; the method is suitable for Magnolia Grandiflora Linn grafting and form directive cultivation.

The invention provides a curing method for the leaf spot disease of magnolia grandiflora. The method comprises the steps of: (1) removing magnolia grandiflora leaves with a disease area over 40% and performing incineration treatment on the leaves in a centralized manner; (2) spraying pesticide liquid on diseased magnolia grandiflora for 15-20 days, once every 3-5 days; (3) every time pesticide liquid is sprayed, spraying a nutrient solution on magnolia grandiflora. The curing method for the leaf spot disease of magnolia grandiflora causes no harm to environment and workers; the pesticide liquid is environmentally-friendly and can effectively cure the leaf spot disease of magnolia grandiflora and prevent spreading of the leaf spot disease.

The invention relates to a cutting seedling-raising method for reducing magnolia grandiflora anthracnose incidence rate. The cutting seedling-raising method particularly includes the steps: (1) high-quality cutting selection; (2) callus tissue treatment; (3) cutting rooting post-treatment. After experiments, the method is used for cutting seedling-raising of magnolia grandiflora, and the incidence rate of anthracnose is reduced from 35.5% in the prior art to 13.6% or less within 6 months before cutting seedling-raising, so that the method has a remarkable effect on improvement of the anthracnose resistance on the magnolia grandiflora.

The invention discloses an organic fertilizer for improving the survival rate of transplanted magnolia grandiflora. The organic fertilizer is prepared from the following raw materials in parts by weight: 50 to 70 parts of animal manure, 45 to 60 parts of plant waste, 50 to 70 parts of kitchen garbage, 10 to 18 parts of plant ash, 20 to 40 parts of biogas residue, 15 to 22 parts of fresh orange peel, 20 to 30 parts of a manure cake, 3 to 8 parts of organic fertilizer fermentation bacteria, 30 to 50 parts of turf and 18 to 25 parts of an acidic fertilizer. The organic fertilizer for improving the survival rate of the transplanted magnolia grandiflora, disclosed by the invention, has a good soil repairing capability; a soil microorganism ecological system is formed, and soil moisture preservation, fertilizer preservation and self fertilizer making functions can be effectively enhanced; the organic fertilizer is used for transplanting the magnolia grandiflora, the survival rate of transplanted seedlings can be effectively improved and the development of root systems is promoted; the capability of preventing plant diseases and insect pests is improved; a preparation method is simple and the preparation cost is low; an application method of the obtained organic fertilizer is worthy of being popularized.

The invention discloses a fast-growing cultivation method for Magnolia grandiflora Linn, belonging to the field of tree cultivation. The method comprises the following steps of: digging a farmland for 60cm deep to prepare the land in winter, conducting permanent planting cultivation to 1-year-old to 2-year-old Magnolia grandiflora Linn grafted seedlings, conducting spaced-plant transplanting within the last ten days of March to the beginning of April after two to three years, conducting pruning in spring and summer within three years after transplanting, keeping the height of a crown to be one third of the height of a tree, ensuring that a trunk does not have crotches at a position below 1.6m to 2.0m, pinching and removing unwanted sprouts in time and focusing on cultivating the trunk. By conducting scientific and effective cultivation management to the Magnolia grandiflora Linn through field planting, transplanting, water manure management, shaping and pruning, the survival rate of the grafted seedlings at an early stage can be well ensured, the development of the root systems of the grafted seedlings and the growth of the trunks are promoted at the same time and the growth speed and the seedling quality of the Magnolia grandiflora Linn are further improved through pruning. By adopting the method, the diameter of the cross-section of the tree trunk of the Magnolia grandiflora Linn can grow 2cm to 3cm every year and can grow to 12cm to 15cm when the Magnolia grandiflora Linn is 5-year to 6-year old.

The invention provides a Magnolia grandiflora seed oil-containing cosmetic with beauty treatment and anti-allergy efficacies and capable of realizing skin allergy prevention and allergy symptom mitigation. The Magnolia grandiflora seed oil-containing cosmetic contains with anti-allergy efficacy contains Magnolia grandiflora seed oil, the Magnolia grandiflora seed oil contains saturated fatty acids, unsaturated fatty acids and other fat-soluble components, the other fat-soluble components include terpene oxygen-containing derivatives, terpenes and esters, the terpenes contain alpha-copaene, caryophyllene and delta-cadinene, and the other fat-soluble components contain, by mass, 2.0-3.0% of alpha-copaene, 4.0-6.0% of caryophyllene and 13.0-15.0% of delta-cadinene.

The invention discloses a special compound slow-release fertilizer for plantation of Magnolia grandiflora. The special compound slow-release fertilizer is composed of the following raw materials by weight: 80-110 parts of decomposed smashed straw, 30-45 parts of decomposed cattle and sheep feces, 22-34 parts of weedtree chips, 10-16 parts of rice washing water, 15-25 parts of biogas residues, 13-20 parts of a boron fertilizer, 10-13 parts of fulvic acid powder, 12-16 parts of diatomite, 11-15 parts of potassium chloride, 4-10 parts of potassium sulfate, 7-9 parts of magnesium sulfate pentahydrate, 17-30 parts of urea, 4-11 parts of clam shell powder, 3-6 parts of a growth regulator and 2-6 parts of a compound microbial inoculant. The special compound slow-release fertilizer of invention uses easily available raw materials, is scientific in formula, reasonable in nutrient collocation and long-lasting in fertilizer efficiency, provides sufficient nutrients for the growth of Magnolia grandiflora, effectively improves the growth efficiency and survival rate of Magnolia grandiflora, and can effectively adjust soil fertility, improve soil structure and greatly prevent soil hardening.

The invention provides an in-vitro rapid propagation method for North American magnolia. The in-vitro rapid propagation method comprises steps of explant selection, explant disinfection, preparation of an initiation culture medium, initiation culture, differentiation culture, cultivation of tissue culture seedlings and seedling hardening. For explant selection, a single magnolia plant is selectedoutdoors, and an explant is cut off at the temperature of 15 DEG C and the humidity of 46%; the cut-off explant is a stem segment with axillary buds, the stem segment is semi-lignified and has two full axillary buds, and the stem segment is 2.8 cm long. For initiation culture, the culture conditions are controlled as follows: the temperature is controlled at 23 DEG C plus or minus 0.5 DEG C, illumination time is 12 h / d, illumination intensity is 2500 Lx, and humidity is 50%. The survival rate of North American magnolia cultivated with the in-vitro rapid propagation method is as high as 99.5%.

The invention discloses a method for breeding a Magnolia grandiflora-magnolia liliiflora hybrid. According to the method, at first, magnolia liliiflora pollen as a male parent obtained in the magnolia liliiflora flowering phase earlier two months than the Magnolia grandiflora flowering phase is preserved and maintains pollen activity by collecting initially open magnolia liliiflora flowers, taking out stamens, uniformly spreading the stamens on a vegetable parchment, naturally drying at a room temperature, after anthers of the stamens crack and pollen is spilled out, collecting the pollen by an iron screen of 80 meshes, drying it in a dry silicon dish by a filter paper bag, closely wrapping the dried pollen by a silver paper, preserving the wrapped pollen under the freezer condition of a temperature of -70 DEG C, and before use, taking out the pollen and carrying out stepwise heating thawing. The method also comprises determination of the best pollination period of Magnolia grandiflora as a female parent, cross pollination reproductive biology basic research, hybrid fruit and hybrid development situation observation and breeding and preservation methods. The method for hybridization can be operated easily and can effectively produce Magnolia grandiflora-magnolia liliiflora hybrid seeds.

The invention provides a honeysuckle essence which consists of a honeysuckle extract, a jasmine extract, synthetic lotus oil, orchid oil, musk ketone, eugenol, a Salvia extract, synthetic orange flower oil, lilial, loquat essence base, piperonal, panaxynol, Magnolia grandiflora fat, Michelia alcohol, Ganoderma lucidum alcohol and ginnol. According to the scheme provided by the invention, the compatible substances in the formula are all natural plant essences; a precise preparation process is also adopted to prepare the honeysuckle essence; and the honeysuckle essence has rich, stable and long-lasting aroma and full and pleasant flavor. The honeysuckle essence has the functions of calming and improving intellegence, supplementing qi and promoting the production of body fluid, regulating the nervous system, clearing away heart fire and tranquilizing the mind, benefiting marrow, nourishing the liver to improve eyesight, clearing heat and eliminating phlegm, clearing away heat and detoxifying, dispelling wind and calming the liver, relieving bruises and pains, activating blood circulation to dissipate blood stasis, treating prickly heat and relieving itching, refreshing oneself, and removing bad smell, thus the purposes of the invention can be achieved.

The invention provides a method for repairing copper and zinc compound contaminated soil in urban relocation land by using woody plants. One or two or more of poplar, camphor trees, Magnolia grandiflora, Magnolia denudata, ligustrum lucidum Ait, Broussonetia papyrifera, Lagerstroemia indica, Prunus Cerasifera, pomegranate trees, hackberry, mulberry trees, plane trees, Zelkova schneideriana, papayatrees, Cerasus yedoensis, Chinese juniper, cedar and elm are adopted, and are planted in the copper and zinc compound contaminated soil. The invention also provides a fertilizer. Pruned branches andleaves or natural fallen leaves of the trees planted in the copper and zinc compound contaminated soil are collected, air-dried and burned, ash obtained after the burning is dissolved in water, the obtained solution is filtered, and the filtered solution is used as the liquid fertilizer after being diluted. The plants are planted in the copper and zinc combined contaminated soil to purify the soil. The pruned branches and leaves or natural fallen leaves of the planted and survived trees are burned and then dissolved in the water, and the obtained solution is used for supplementing inorganic nutrients in the growth and development process of the plants as the liquid fertilizer, so the method is environmentally friendly and economical.

The invention relates to an extraction and preparation method of parthenolide, and particulary relates to an extraction method of parthenolide from Magnolia grandiflora root bark. The extraction and preparation method comprises: crushing Magnolia grandiflora root bark as a raw material, extracting with an appropriate polar organic solvent to obtain total lactones, separating the total lactones by column chromatography, collecting the required ingredients, and then crystallizing the collected ingredients with an appropriate solvent to obtain parthenolide. The extraction method of parthenolide from Magnolia grandiflora root bark provided by the invention is suitable for industrial production.

The invention discloses an environmentally-friendly special fertilizer for cultivating Magnolia grandiflora. The special fertilizer comprises, by weight, 10-50 parts of ammonium chloride, 6-28 parts of ferrous sulfate, 4-24 parts of EDTA chelated iron, 5-25 parts of cysteine, 10-40 parts of ammonium phosphate, 6-28 parts of nitrilotriacetic acid, 4-28 parts of urea, 6-28 parts of potassium chloride, 3-21 parts of bean cake powder, 3-21 parts of polyaspartic acid, 5-25 parts of Astragalus sinicus straws, 6-28 parts of rice stems, 6-28 parts of indolebutyric acid, 4-28 parts of carbendazim, 5-25 parts of Chinese angelica root, 4-16 parts of medicated leaven, 4-18 parts of sodium carbonate, 6-28 parts of durian shells, 6-22 parts of sodium carbonate, 4-18 parts of Herba Schizonepetae and 3-21 parts of tobacco leaves. The special fertilizer has the advantages of good environmental protection performance, balanced nutrition and promotion of growth of Magnolia grandiflora.

The invention discloses a plantation method capable of preventing magnolia grandiflora from lodging, which comprises the following steps of: field selection; planting; field management; digging a drainage trench in flat magnolia grandiflora fields or farmlands every 5-6m, wherein the drainage trench is 30-40cm in depth and 20-25cm in width. The method disclosed by the invention is a new method firstly adopted in the production for preventing the magnolia grandiflora from lodging, and makes up the blank of a method for preventing the magnolia grandiflora from lodging in the prior art. After being adopted, the method for preventing the magnolia grandiflora from lodging can obviously reduce wind-fall rare of the magnolia grandiflora in the flat field or farmland in a large scale by over 95%; the loss of large seedlings can be obviously reduced; the economic loss of the production operators of seedlings can be greatly relieved; in addition, the seedlings can grow strongly and compared with the prior art, the growth indicator of the seedlings is over 15%; the cultured seedlings are thicker and more strong, the degree of lignification is high, plant diseases and insects pests are very few, the seedling outplanting is high, the planting survival rate of the seedlings provided for greening is high, and the adaptability is strong. The method disclosed by the invention is simple and feasible, low in cost, and easy in population and application.

The present invention relates to a lobule dwarf type magnolia grandiflora tender stem segment isolated culture method, and belongs to the technical field of plant tissue culture. The method comprises: 1) selecting and pre-treating a material to be inoculated, 2) sterilizing an explant, 3) establishing a sterile system, 4) carrying out induction and proliferation culture on clustered shoots, 5) carrying out strong seedling and rooting culture, and 6) carrying out seedling exercising transplantation on test-tube seedlings. The lobule dwarf type magnolia grandiflora tender stem segment isolated culture method has the following beneficial effects that: the clustered shoot induction rate achieves more than 90%, the rooting rate after the strong seedling and rooting culture achieves more than 96%, the survival rate can achieve more than 96%, advantages of rapid reproduction, high reproduction rate, virus removal, purification rejuvenation and the like are provided, the defects of low reproduction coefficient, long period, seasonal restriction and the like in the prior art are overcome, and the high quality seedling can be produced within a short time in a factory manner so as to meet requirements of rural and urban greening. In addition, the one stem segment is adopted as the sample, tens of thousands of the seedlings can be rapidly propagated within a year in the factory manner, and the quality is excellent.