The invention discloses a high-yield disease-resistant loach breeding feed and a making method thereof. The high-yield disease-resistant loach breeding feed comprises the following raw materials in parts by weight:10-15 parts of peanut meal, 10-15 parts of soybean meal, 8-10 parts of carrot root powder, 8-10 parts of powder of radix rosae multiflorae, 5-7 parts of spirulina powder, 10-12 parts of powder of radix et rhizoma rhei, radix scutellariae and cortex phellodendri, 15-20 parts of corn flour, 13-15 parts of sorghum flour, 13-15 parts of unhulled rice powder, 10-12 parts of peanut powder, 7-9 parts of soybean protein powder, 7-8 parts of rapeseed meal powder, 20-25 parts of fish meal, 18-20 parts of shrimp powder, 15-17 parts of silkworm chrysalis meal, 5-8 parts of pig's blood powder, 8-10 parts of fly larva powder, 5-8 parts of a nutritive mixture, 5-7 parts of composite multi-minerals, 8-10 parts of a Chinese herbal medicine additive, and 3-5 parts of table salt. In a formula of the loach breeding feed, materials are pollution-free substances, and are green and environmental-friendly, so that the loach breeding feed is an ecological environmental-friendly green feed. According to loaches fed with the feed, the disease resistance is obviously improved, the survival rate is greatly increased, the loaches are high in reproduction speed, the yield is also increased, the discharge of hazardous substances is reduced, and stability of water quality is maintained, favorable water body environment of the loaches is guaranteed, and the healthy growth of the loaches is promoted.