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Method for microalgae breeding and biological oil co-production

A microalgae and parallel technology, applied in the field of microalgae cultivation, can solve the problems of low photoautotrophic growth efficiency and dependence on external addition of organic carbon, and achieve high growth efficiency and fast growth speed

Inactive Publication Date: 2013-07-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] None of the existing technologies can solve the low efficiency of photoautotrophic growth and the dependence of organic carbon in heterotrophs on external additions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Collected 18g of golden algae powder was added with 90ml of 5% (weight) hydrochloric acid solution, heated at normal pressure and temperature at 50°C for 2hr, and filtered to obtain 6.3g of filter cake with a hydrolysis rate of 65%. The filter cake was extracted with n-hexane to obtain 3.3 g of extract, which was slightly black bio-oil. After the filtrate is neutralized with 10% (weight) aqueous NaOH solution, the initial concentration is 5×10 6 In the 10L microalgae culture solution of each cell per milliliter, the photobiological reaction device is a box-type reactor made of hard borosilicate glass, and 2-4 30W fluorescent tubes are evenly distributed around the reactor, and the constant temperature water bath is controlled at 25-30 ℃, with 3wt% CO 2 Air mixture, the gas flow rate is 200ml / min. The microalgae grow rapidly, and the density is 4×10 after 3 days of culture 7 cells per milliliter, the density was 1.77g / L after 7 days, and 17.7g of algae powder was obta...

Embodiment 2

[0032] 18g of the collected chlorella algae powder was added to 90ml of 5% (weight) hydrochloric acid solution, and heated to reflux for 2 hours under normal pressure and temperature of 100°C to obtain 7.3g of filter cake with a hydrolysis rate of 59.4%. The filter cake was extracted with n-hexane, and the n-hexane was rotary evaporated to obtain 1.82 g of product. After the filtrate was neutralized with 10% NaOH aqueous solution, the initial concentration was 5×10 6 In the 10L microalgae culture medium per milliliter, the photobiological reaction device is a pipeline reactor made of hard borosilicate glass, with 2-4 30W fluorescent tubes evenly distributed around the reactor, and the constant temperature water bath is controlled at 25-30°C , fed with 3wt% CO 2 Air mixture, the gas flow rate is 200ml / min. The microalgae grew rapidly, and the density was 1.69g / L after 7 days of culture, and 16.9g of algae powder was obtained after collection and drying.

Embodiment 3

[0034] Add 18 g of Chrysophylla algae powder collected, add 90 ml of 5% sulfuric acid solution, heat at normal pressure and temperature at 80° C. for 2 hr, and filter to obtain 7.7 g of filter cake with a hydrolysis rate of 57%. The filter cake was extracted with n-hexane to obtain 3.0 g of extract, which was slightly black bio-oil. After the filtrate was neutralized, the initial concentration was 5×10 6 In the 10L microalgae culture medium of each cell per milliliter, the photobiological reaction device is made of hard borosilicate glass, and 2-4 30W fluorescent tubes are evenly distributed around the reactor. %CO 2 Air mixture, the gas flow rate is 200ml / min. The microalgae grew rapidly, and the density was 3.4×10 after 3 days of culture 7 cells per milliliter, the density was 1.47g / L after 7 days, and 14.7g of algae powder was obtained after collection and drying.

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Abstract

The present invention relates to a novel method for microalgae circulation breeding and biological oil co-production, wherein additional nutrition substances are not required to be added, and the method can be provided for low cost microalgae culture and biological oil co-production. The method mainly comprises a novel microalgae circulation breeding manner and application of the circulation breeding process to co-produce the biological oil, wherein the microalgae collected from each generation breeding is subjected to hydrolysis to obtain a water phase and an oil phase, the water phase is adopted as a microalgae circulation breeding nutrition liquid to be added to a microalgae breeding system so as to provide a carbon source, a nitrogen source, a phosphorus source and inorganic salts, and achieve a purpose of circulation breeding, and fatty acids contained in the oil phase are the products. The method for microalgae breeding has characteristics of high efficiency, rapid microalgae growth, no requirement of additional nitrogen source, phosphorus source and inorganic salts, biological oil co-production, high economic values and high industrial application prospects.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and in particular relates to a method suitable for cultivating microalgae and other photosynthetic biological cells and co-producing bio-oil. Background technique [0002] In today's world where fossil resources are increasingly depleted, the search for alternative green energy has received widespread attention. Microalgae is a kind of biological resources with great application value. [0003] At present, there are three main nutrition modes of microalgae: photoautotrophy, heterotrophy and mixed nutrition. The photoautotrophic model uses sunlight as energy, absorbs carbon dioxide as an inorganic carbon source, and adds inorganic salts to the nutrient solution for cultivation. Organic matter such as glucose can also be used to make microalgae grow heterotrophically. Mixotrophy adds organic matter to cultivate microalgae in a photobioreactor. The cost of photoautotrophic culture of microa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12R1/89C12N1/12C12P7/6463C12P7/64
Inventor 徐云鹏董兴隆刘中民薛松孙新德白长敏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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