40 results about "Golden algae" patented technology

The invention discloses a culture method of golden algae, which belongs to the water pollution control field. The invention takes a plant leaching liquor as an organic matter culture medium to culture the golden algae under the condition of room temperature and natural illumination or being put into an artificial climate box, and after the collection, the golden algae is used for controlling the water bloom. The phagocytosis experiment of water bloom algae indicates that the golden algae obtained by the method can be applied to the control of the water bloom. The culture medium used by the method of the invention for culturing the golden algae adopts the raw materials which are easy to be obtained, and have low cost, less energy consumption and high output, and no specific equipment is needed in the culture, thus being easy to be realized; and the cultured golden algae satisfies the requirement on application in the control of water bloom algae.

Disclosed are a preparing method and its product and application. Firstly, condense the pelagic unicellular alga in 1~2 degree, which is made into cream-condensed alga product, the dry it in low temperature or in vacuum, making the products which will not become harmful substance to juvenile of animal culture and can be diffused into alga juvenile of animal culture single cell with complete cell, finally, package it in vacuum or in nitrogen. The invention can be applied in all kinds of pelagic unicellular alga, and the products includes chlorella, nitzschia, chaetocero, can replace live pelagic unicellular alga to be applied in culture of seedling of animal culture in water, cultivation and strengthening of diet animal, especially for feeding diet animal .

The invention relates to a golden algae (Poterioochromonas malhamensis), which is preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee with the preservation number CGMCC No. 11620. The present invention also relates to a method for cultivating Chrysophytes, which includes adding bran-free grains of grains and optionally nutrient salts and vitamins as nutrient sources for the growth of Chrysophytes into an aqueous system for cultivating Chrysophytes. The grain is selected from one or more of the following groups: rice, wheat, millet, black rice, buckwheat, oat, barley and sorghum, preferably rice and wheat. The nutrients and vitamins include NaNO 3 、KH 2 PO 4 、K 2 HPO 4 , Vitamin B 6 and vitamin B 12 . The isolated golden algal strain of the invention is easy to cultivate and grows rapidly. The cultivation method used in the invention is simple, the raw materials used for cultivation are cheap, and the cultivation cost is greatly reduced. At the same time, the method is established under the condition of bacteria and is very suitable for large-scale cultivation application.

The invention relates to the field of microalgae biotechnology, in particular to a method for concentrating and harvesting Isoflagellates by using ethanol and realizing semi-continuous culture, adding ethanol to the algae liquid of Isoflagellates waiting to be harvested after cultivation After standing still for 18-24 hours, the algae cells of Isochrysis globosa that settled at the bottom of the container were harvested by siphon method, and at the same time, new culture medium was added to the remaining algae liquid for re-inflated culture, so as to realize the semi- Continuous culture. Using extremely low concentration of ethanol to treat dinoflagellate algae to make them lose their ability to move, the majority of dinoflagellate cells in the algae liquid can be harvested by precipitation method, the recovery rate is as high as 70%, and the added Low ethanol concentration is very volatile, the harvested microalgae cells are safe and non-toxic, good resuspension performance, low cost of ethanol, sedimented algae cells siphon the residual dinoflagellate cells in the algae liquid after harvesting under aerated conditions Supplementing with new culture medium can still continue to proliferate, realizing the semi-continuous culture of Isoflagellates.

The invention provides a mixing fermentation method of chrysophyceae and photosynthetic bacteria, which belongs to the field of biotechnology. The invention concretely provides the mixing fermentation method of isochrysis galbana and ectothiorhodospira shaposhnikovii, isochrysis galbana and ectothiorhodospira shaposhnikovii are respectively subjected to seed activation, culture, and then mixing fermentation in an illuminating fermentation tank, so that isochrysis galbana content is greatly increased which is 5-10 times of that through normal culture.

The invention provides a method for preserving Isochrysis globosa, which belongs to the technical field of microalgae cultivation. The method comprises the following steps: 1) Cultivating Isochrysis globosa with an f / 2 medium containing a mixed antifreeze agent. Obtaining Isochrysis globosa culture medium in the logarithmic growth phase; 2) Adding ferric chloride to the Isochrysis globosa culture fluid described in step 1) for flocculation to obtain flocculated Isochrysis globosa cells; 3) The flocculated Isochrysis globosa cells are collected, freeze-dried, and sealed for storage; the mixed antifreeze agent in step 1) includes dimethyl sulfoxide and methanol. Using the method of the present invention to preserve Isochrysis globosa, after one year of storage at room temperature, the survival rate of the Isochrysis globosa cells can reach 92%, which is far greater than the cell survival rate of conventional preservation methods.

The embodiment of the invention discloses intelligentized illumination equipment and a spectra modulation method for golden alga culture. The intelligentized illumination equipment comprises a storage module, a judgment module, a duty ratio acquisition module and a light source module, wherein the storage module records the growth parameters of golden algae; the judgment module judges the current growth state of the golden algae; the duty ratio acquisition module acquires the growth characteristic parameters corresponding to the current growth state of the golden algae according to the current growth state of the golden algae and acquires blue-green light duty ratio corresponding to blue-green light currently needed by the golden algae and red light duty ratio corresponding to red light according to the growth characteristic parameters; and the light source module generates a corresponding blue-green light PWM (Pulse Width Modulation) signal according to the blue-green light duty ratio, generates the blue-green light with corresponding illumination intensity according to the blue-green light PWM signal, generates a corresponding red light PWM signal according to the red light duty ratio and generates the red light with corresponding illumination intensity according to the red light PWM signal. The intelligentized illumination equipment and the spectra modulation method which are provided by the embodiment of the invention can be used for increasing the light energy utilization efficiency of the golden algae on a light source, thereby increasing the growth speed of the golden algae.

The invention belongs to the technical field of bioengineering, and particularly relates to a method for improving the culture density of isochrysis galbana. The method comprises the steps: activatinga marinobacter aquaeolei strain; culturing a marinobacter aquaeolei solution; carrying out sterile treatment on isochrysis galbana species; and producing an isochrysis galbana seed culture solution,wherein the density of isochrysis galbana is more than or equal to 10*10<5> / mL, and according to formal culture production of isochrysis galbana, the density of isochrysis galbana is 1.5*10<7> cell / mLor above. The isochrysis galbana and marinobacter aquaeolei co-culture system can generate a synergistic effect, so that the components of a culture medium can be fully utilized, and the isochrysis galbana and the marinobacter aquaeolei simultaneously benefit from each other and promote co-growth due to the physiological and ecological characteristics of the isochrysis galbana and the marinobacter aquaeolei. Under the condition of mixed culture of the isochrysis galbana and the marinobacter aquaeolei, the isochrysis galbana can continuously and quickly grow, the biomass higher than that of aconventional method is finally obtained, and effective technical support can be provided for related aquaculture baits and DHA-obtaining algae.