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Golden algae and its cultivation method

A cultivation method, the technology of golden algae, which is applied in the field of microalgae industrial application, can solve the problems that it is not suitable for large-scale industrial cultivation, and achieve the effects of reduced cultivation cost, rapid growth and low price

Active Publication Date: 2020-06-16
GUOTOU BIO TECH INVESTMENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, currently known species and strains of golden algae are not suitable for large-scale industrial cultivation

Method used

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  • Golden algae and its cultivation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1. Isolation and identification of Chrysophylla CMBB-01 strain

[0039] 1. Isolation and purification of CMBB-01 strain

[0040] The algae liquid containing the algae strain of the present invention comes from the natural environment water body, and is further separated by a serial dilution method to obtain a pure algae strain, which is numbered and named as CMBB-01 strain.

[0041] 2. Morphological identification of CMBB-01 strain

[0042] The morphological and structural characteristics were observed by optical microscope (OLYPUS, BX53, 400 times) and scanning electron microscope (SEM). Such as Figure 1A and 1B As shown, the cell diameter of CMBB-01 strain is 5-10 μm, and contains two flagella of different sizes. 1-1.5μm, short flagella without villi; cells of CMBB-01 strain contain perichrome, yellow or golden brown.

[0043] The CMBB-01 strain belongs to the mixed trophic type. It can grow autotrophically through photosynthesis in inorganic salt me...

Embodiment 2

[0053] Embodiment 2. The mensuration of CMBB-01 strain growth performance under different nutrient source conditions

[0054] 2.1. Using rice grains without bran as nutrient source

[0055] The golden algae is cultivated by adopting the bran-removed rice grains as the nutrient source of the golden algae strain of the present invention. At the same time, in order to study the effects of different amounts of rice grains on the growth of Chrysophyta, different amounts of rice grains were added to the same culture system, and five groups with different amounts of rice grains were set up: 0.25g / L, 2.5g / L L, 12.5g / L, 25g / L and 50g / L. 4 repetitions were set for each group. In order to ensure that the golden algae did not completely sink to the bottom, the flask was shaken four times a day. Samples were taken regularly every day and counted using a hemocytometer. Each sample was counted twice, and the average value of four repeated samples was taken as the final value. The init...

Embodiment 3

[0072] Embodiment 3. The influence of light on the growth of CMBB-01 strain

[0073] For researching the influence of illumination on the growth of golden algae of the present invention, light group 1 (50 μ molphotons·m -2 ·s -1 ), light group 2 (100μmol photons·m -2 ·s -1 ) and complete darkness group (0μmolphotons·m -2 ·s -1 ). Each group adopts the wheat grains+nutrient salt+vitamins in the part 2.3 of the above-mentioned embodiment as the nutrient source. The remaining experimental conditions and methods are the same as those in Section 2.1 above.

[0074] Such as Figure 7 As shown, even in the absence of light, the Chrysophylla strain of the present invention uses wheat grains + nutrient salts + vitamins as a nutrient source, and its density can reach 10 when it is cultivated for 3 days. 6individual / mL. This suggests that the growth performance of the Chrysophylla strain of the present invention is very excellent. From the 2nd day of culture, under the condit...

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Abstract

The invention relates to a golden algae (Poterioochromonas malhamensis), which is preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee with the preservation number CGMCC No. 11620. The present invention also relates to a method for cultivating Chrysophytes, which includes adding bran-free grains of grains and optionally nutrient salts and vitamins as nutrient sources for the growth of Chrysophytes into an aqueous system for cultivating Chrysophytes. The grain is selected from one or more of the following groups: rice, wheat, millet, black rice, buckwheat, oat, barley and sorghum, preferably rice and wheat. The nutrients and vitamins include NaNO 3 、KH 2 PO 4 、K 2 HPO 4 , Vitamin B 6 and vitamin B 12 . The isolated golden algal strain of the invention is easy to cultivate and grows rapidly. The cultivation method used in the invention is simple, the raw materials used for cultivation are cheap, and the cultivation cost is greatly reduced. At the same time, the method is established under the condition of bacteria and is very suitable for large-scale cultivation application.

Description

technical field [0001] This application relates to the field of industrial application of microalgae, specifically, a newly isolated golden algae, Poterioochromonas malhamensis CMBB-01 strain, and its cultivation method. Background technique [0002] Golden algae, also known as golden brown algae, are mostly distributed in fresh water and also in sea water. The cells of golden algae are rich in nutrients, and golden algae laminarose is the energy storage substance of its cells. It has a wide range of biological activities and has a certain preventive effect on gout, hyperlipidemia, and early and middle stage renal failure. At the same time, golden algae is also a good fish bait and has a wide range of uses in the aquaculture industry. In addition, studies have shown that golden algae can effectively swallow a variety of bloom algae including Microcystis aeruginosa, so it also has potential uses in water eutrophication control. [0003] The traditional method of autotrophic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12C12N1/125C12R2001/89
Inventor 龚迎春马明洋胡强
Owner GUOTOU BIO TECH INVESTMENT CO LTD
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