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Culture method for improving golden algae biomass and grease content by using nitrogen source

A technology of oil content and cultivation method, which is applied in the field of marine biology to achieve the effects of strong light adaptability, high value of by-products and good application prospects

Inactive Publication Date: 2012-05-16
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

It is reported that the total fat in Chrysophytes, especially Isochrysis globosa, can account for 20-40% of its dry weight, and is rich in polyunsaturated fatty acids, but so far there has been no report on Isochrysis zhanjiang

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  • Culture method for improving golden algae biomass and grease content by using nitrogen source

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Embodiment 1

[0033] Firstly, the Zhanjiang Isochrysis algae cells were pre-cultured in a 3L Erlenmeyer flask for shaking flask culture, the culture volume was 2L, and they were grown to the exponential phase on the light culture rack, and the algae cell density was 600-700×10 4 cell.ml -1 , the algae fluid was centrifuged to collect the algae cells.

[0034] Centrifuge the algae liquid in the exponential phase and inoculate it in sterilized f / 2 medium, cultivate it in a 600ml columnar photobioreactor with a diameter of 5cm, the culture volume is 500ml, and the final inoculation concentration is 8×10 6 cell ml -1 . Sodium nitrate was used as the nitrogen source, and no nitrogen source was added during the cultivation process. Phosphorus sources, metal elements, and vitamins were supplemented once every 72 hours. ℃, light intensity 200μmol·m -2 the s -1 , the light-to-dark ratio is 14h:10h under the condition of passing CO 2 Cultivate, CO 2 The concentration was 2%, the aeration rate ...

Embodiment 2

[0037] Firstly, the Zhanjiang Isochrysis algae cells were pre-cultured in a 3L Erlenmeyer flask for shaking flask culture, the culture volume was 2L, and they were grown to the exponential phase on the light culture rack, and the algae cell density was 600-700×104 cell ml -1 , the algae fluid was centrifuged to collect the algae cells.

[0038] Centrifuge the algae liquid in the exponential phase and inoculate it in sterilized f / 2 medium, cultivate it in a 600ml columnar photobioreactor with a diameter of 5cm, the culture volume is 500ml, and the final inoculation concentration is 8×10 6 cell ml -1 . The nitrogen source was urea, and 0.03 g / L urea was added to the culture system every 24 hours, and the phosphorus source, metal elements, and vitamins were supplemented once every 72 hours. ±1℃, light intensity 200μmol.m -2 the s -1 , the light-to-dark ratio is 14h:10h under the condition of passing CO 2 Cultivate, CO 2 The concentration was 2%, the aeration rate was 0.8vvm...

Embodiment 3

[0041] Firstly, the Zhanjiang Isochrysis algae cells were pre-cultured in a 3L Erlenmeyer flask for shaking flask culture, the culture volume was 2L, and they were grown to the exponential phase on the light culture rack, and the algae cell density was 600-700×10 4 cell.ml -1 , the algae fluid was centrifuged to collect the algae cells.

[0042] Centrifuge the algae liquid in the exponential phase and inoculate it in sterilized f / 2 medium, cultivate it in a 600ml columnar photobioreactor with a diameter of 5cm, the culture volume is 500ml, and the final inoculation concentration is 8×10 6 cell.ml -1 . Sodium nitrate was selected as nitrogen source, 4.5g / L sodium nitrate was added to the culture system every 24 hours, and phosphorus source, metal elements and vitamins were supplemented once every 72 hours. Temperature 25±1℃, light intensity 200μmol.m -2 the s -1 , the light-to-dark ratio is 14h:10h under the condition of passing CO 2 Cultivate, CO 2 The concentration was...

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Abstract

The invention relates to a culture method for improving golden algae biomass and grease content, and discloses a culture regulation and control method for improving the golden algae biomass and the grease content simultaneously by using a nitrogen source. The method comprises the following steps of: inoculating algae strains into the conventional f / 2 sterilization culture medium at high density; adding a nitrogen source into a culture system every 12 to 72 hours; introducing CO2 for culturing at the concentration of 0.2 to 100mmol / L, the culture temperature of between 25 and 30 DEG C, the light intensity of 100 to 300mu mol.m<-2>s<-1> and the air introduction speed of 0.6 to 1.2vvm in the light-dark ratio of 14h to 10h; and harvesting within 8 to 10 days, wherein the highest density of algae cells can reach 1.3*10<8> cell / ml, the maximum dry weight can reach 3.12g / L, and the maximum grease content can reach 53 percent. By the method, high biomass is acquired, high grease content of the algae cells is realized, and the defects of nitrogen limitation on improvement of the grease content of the algae cells and reduction in the biomass at present are overcome.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to the growth and cultivation of marine single-cell microalgae Chrysophylla and oil regulation. Background technique [0002] At present, energy issues have become the main issues leading the country's economic development and strategic security. According to the current proven reserves and consumption rate, the world's oil reserves can only last for 40 years. In addition, the consumption of traditional fossil energy mainly based on petroleum has brought serious pollution to the environment. 70% of CO2 and suspended particles in the air come from the combustion of various fossil fuels. Therefore, the decrease of fossil energy and the environmental pollution caused by it make researchers focus on the development and utilization of renewable energy. Among various renewable energy sources, biomass energy is the most common renewable energy resource with extensive...

Claims

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Application Information

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IPC IPC(8): C12N1/12
Inventor 薛松冯迪娜陈兆安张卫陆洪斌
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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