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Marine microalga and its culture method and application

A technology of marine microalgae and cultivation methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of reducing environmental pollution

Active Publication Date: 2013-08-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most domestic biomass energy research focuses on the screening of biodiesel raw materials, the distribution, selection, cultivation, genetic improvement and processing technology and equipment of oily plants, and does not involve marine microalgae biomass energy.

Method used

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  • Marine microalga and its culture method and application
  • Marine microalga and its culture method and application
  • Marine microalga and its culture method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The marine microalgae is Isochrysis sp.CCMM5001, which was deposited in China Center for Type Culture Collection (CCTCC) on June 7, 2010, with the preservation number CCTCC NO: M2010138.

[0017] Isochrysis sp.CCMM5001 belongs to Chryspohyta, Chrysophyceae, Iochysridales, Isochysidacecae, etc. Ischrysis Parke and Ischrysis globosa are naked motile cells, the cell shape is variable, generally oblong or elliptical, and the young cells have a slightly flat dorsal and ventral surface, so the side view is elongated Oval. Generally, active cells are 5-6 μm long, 2-4 μm wide, 2.2-3 μm thick, cyst diameter 5-6 μm, no cell wall, two flagella are equal in length, starting from the front of the cell, its length is 1-2 times that of the cell. Normally cells move slowly, spinning and whirling at a rate of about one rotation per second. When the cell is at rest, the flagella is rigid and falls down from the body, but when it is in motion, the flagella is directed toward the front o...

Embodiment 2

[0036] Analysis of total fat percentage:

[0037] Take the freeze-dried algal powder of Isoflagellates obtained above, weigh a certain weight (0.5-1 g), and add distilled water. Freeze it at -20°C, then dissolve it in a water bath at 40°C for 5 minutes, repeat freezing and dissolving twice, and observe the broken state under a microscope (see figure 1 ). After dissolving, add 10ml of a mixed solvent of anhydrous ether and petroleum ether with a volume ratio of 1:2 to the algae liquid, shake and mix well, then add 5ml of KOH with a mass fraction of 10% to precipitate the algae cells, and then dissolve the algae cells at 4000r min -1 Centrifuge for 15 minutes under certain conditions, and take the supernatant. Evaporate the excess solvent quickly in a water bath at 60°C to constant weight, weigh the crude fat content, and use the following formula to calculate the percentage of total fat (see Table 1).

[0038] Total fat percentage = (total fat / dry algae weight)×100%

[003...

Embodiment 3

[0043] Analysis of Fatty Acid Components in Chrysophylla

[0044] Add 5mL 0.4mol L to the dried above crude fat sample of Example 2 -1 KOH-CH 3 OH solution, saponified by heating in a water bath at 60°C for 2h until the oil droplets completely disappeared. After cooling, move it into a centrifuge tube, add hydrochloric acid (9:1=commercial concentrated hydrochloric acid:distilled water) to adjust the pH to 1-2, then add 1mL of 5% NaCl solution, and then use 6ml of CHCl with a volume ratio of 1:4 3 -C 6 h 14 Extract the mixed solution and take the organic phase. Add 5 mL of BF with a mass fraction of 14% in the organic phase 3 -CH 3 OH solution was methylated at 60°C for 1h. Blow dry with nitrogen, make up to volume with 2mL of n-hexane, take 1mL of the solution in a sample bottle, and perform GC / MS detection (see Table 2). The experimental instrument is an Agilent 7890N gas chromatograph-975C mass spectrometer (GC / MS). The chromatographic column is: HP-5MS, (30m×0.25m...

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Abstract

The invention relates to a marine microalga and its culture method and application. The marine microalga is isochrysis sp. CCMM5001, is preserved in the china center for type culture collection (CCTCC), and has a preservation number of CCTCC NO:M2010138. The culture method comprises separating a single algae cell of isochrysis sp. CCMM5001, carrying out enlargement culture of the single algae cell under laboratory conditions, carrying out centrifugation in a fourth day after an isochrysis sp. CCMM5001 growth platform stage, and then drying to obtain isochrysis sp. CCMM5001 powder. The isochrysissp. CCMM5001 powder can be utilized as a biodiesel raw material. The culture method of the marine microalga utilizes carbon dioxide in industrial exhaust gas thereby reducing greenhouse gas discharge, and realizes absorption of oxynitrides in industrial exhaust gas thereby reducing environmental pollution.

Description

technical field [0001] The invention relates to marine microalgae, in particular to a marine microalgae and its cultivation method and application. Background technique [0002] Marine microalgae is the main provider of organic matter and energy in the marine ecosystem. Compared with other raw materials, it has high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and is not restricted by climate. It can biochemically synthesize oil itself, and oil High content, easy to achieve large-scale production and other advantages, so marine microalgae show broad application prospects as a new source of biodiesel fuel. Develop marine microalgae resources, using seawater, non-cultivated land and solar energy to combine simple mineral nutrients and CO 2 Converting it into oil has very good application and development prospects, and has become one of the hot issues in international scientific and technological competition. The research began in the 1940...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/13Y02E50/10
Inventor 韩笑天郑立俞志明邹景忠
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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