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Cultivation method of golden algae

A cultivation method and technology of golden algae, applied in the field of algae cultivation, can solve the problems of low economy, unsatisfactory growth of golden algae, low biomass, etc., and achieve the effects of less energy consumption, easy realization and high yield

Inactive Publication Date: 2010-09-29
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the composition of commonly used inorganic salt medium is relatively stable, the growth of this strain of Chrysophylla in several commonly used inorganic salt medium is not ideal, and the obtainable biomass is low, while the organic medium of commonly used protozoa generally uses wheat Extracts of grains, rice, etc., this type of medium can be used to cultivate Chrysophylla ZX1, but the economy is not high

Method used

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  • Cultivation method of golden algae
  • Cultivation method of golden algae
  • Cultivation method of golden algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Select five kinds of aquatic plants of Alisma, water willow, cattail, reed bamboo and reed, weigh the plants with corresponding dry weight, crush or chop them, soak them in water and boil them for 20 minutes according to the plant dry weight concentration of 10g / L to prepare Take the plant extract, centrifuge or filter to remove plant residues, and sterilize at 121°C for 30 minutes before cultivating Chrysophylla ZX1. At the same time, 10 g / L (dry weight concentration) of wheat grain extract was prepared as a common organic medium, and the growth of Chrysophylla ZX1 in the wheat grain extract was used as a control. Chrysophyte species ZX1 (4×10 3 pcs mL -1 ), placed in an artificial climate box for cultivation (artificial climate box cultivation conditions: the light-dark ratio is 14h:10h, the temperature is 25°C, the relative humidity is 75%, and the light intensity is 800-1300lux). The growth curves of golden algae in each culture medium are as follows: figure 1 s...

Embodiment 2

[0024] Respectively with heating extraction method (with embodiment 1) and soaking extraction method, the reed extract solution that preparation dry weight concentration is 10g / L, wherein soaking method step comprises (1) take by weighing 1g dry reed, cut into 1- (2) Immerse it in 100mL tap water for 5 days, filter to remove the residue, and sterilize at 121°C for 30 minutes before use. In the reed extracts produced by the two methods, the initial concentration of inoculation was about 10 4 pcs mL -1 Chrysophylla ZX1 was cultured in an artificial climate chamber (artificial climate chamber cultivation conditions: light-dark ratio 14h:10h, temperature 25°C, relative humidity 75%, light intensity 800-1300lux). The growth curves of Chrysophytes in the two cases are as follows image 3 shown. It can be seen from the figure that in the extracts obtained by the two extraction methods, the growth of golden algae is similar, and there is no obvious difference. Therefore, both heat...

Embodiment 3

[0026] Prepare the reed extract solution of different dry weight concentrations with heating extraction method (with embodiment 1), and dry weight concentration is respectively 0.5, 1, 5, 10, 20, 50g / L, and golden algae initial density is about 8.6 * 10 3 pcs mL -1 , placed in an artificial climate chamber for cultivation (artificial climate chamber cultivation conditions: the light-dark ratio is 14h:10h, the temperature is 25°C, the relative humidity is 75%, and the light intensity is about 800-1300lux). The growth of golden algae in each culture medium is as follows: Figure 4 shown. Under the condition that the initial density of Chrysophylla species is constant and the culture time is the same, when the concentration of the reed extract is in the range of 0.5-20g / L, the density of Chrysophylla increases with the increase of the concentration of the culture solution, but when the concentration is too high (50g / L), the color of the culture solution is too dark, which affe...

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Abstract

The invention discloses a culture method of golden algae, which belongs to the water pollution control field. The invention takes a plant leaching liquor as an organic matter culture medium to culture the golden algae under the condition of room temperature and natural illumination or being put into an artificial climate box, and after the collection, the golden algae is used for controlling the water bloom. The phagocytosis experiment of water bloom algae indicates that the golden algae obtained by the method can be applied to the control of the water bloom. The culture medium used by the method of the invention for culturing the golden algae adopts the raw materials which are easy to be obtained, and have low cost, less energy consumption and high output, and no specific equipment is needed in the culture, thus being easy to be realized; and the cultured golden algae satisfies the requirement on application in the control of water bloom algae.

Description

technical field [0001] The invention relates to a method for cultivating algae, in particular to an economical method for cultivating golden algae that can engulf various algae blooms (such as Microcystis aeruginosa, Sagittarius terrestris, and Chlamydomonas reinhardtii). Background technique [0002] The excessive nitrogen and phosphorus content in the water body leads to eutrophication of the water body, which in turn leads to the excessive reproduction of algae to form algae blooms, and makes the water body lose its functions such as industrial and agricultural use, landscape, and breeding. The most widely used method of inhibiting algae is copper ion (Cu 2+ ) algicides. Although this type of algicide has a significant algicidal effect, it will also kill other aquatic plants and animals in the water body, destroy the aquatic ecological environment, and will produce bioaccumulation effects in the aquatic ecosystem, or increase the amount of water in the lake sediment. co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89
CPCY02W10/10
Inventor 胡洪营张薛黄璜
Owner TSINGHUA UNIV
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