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Culture method of golden algae and application thereof in controlling water-bloom algae aspect

A technology of blooming algae and golden algae, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of destroying the aquatic ecological environment, increasing the content of harmful substances in lake sediments, etc. The effect of good ecological safety, easy cultivation and easy utilization

Inactive Publication Date: 2011-11-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this type of algicide has a significant algicidal effect, it will also kill other aquatic plants and animals in the water body, destroy the aquatic ecological environment, and will produce bioaccumulation effects in the aquatic ecosystem, or increase the amount of water in the lake sediment. content of harmful substances

Method used

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  • Culture method of golden algae and application thereof in controlling water-bloom algae aspect
  • Culture method of golden algae and application thereof in controlling water-bloom algae aspect
  • Culture method of golden algae and application thereof in controlling water-bloom algae aspect

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A kind of gold algae, its culture method is, cultivate in artificial climate box, light-dark ratio is 14h:10h, temperature is 25 ℃, relative humidity is 75%, adopts the following steps under the condition of light intensity 800~1300lux:

[0021] (1) prepare A5 solution, with H 3 BO 3 , MnCl 2 .4H 2 O, ZnSO 4 ·7H 2 O, CuSO 4 ·5H 2 O, Na 2 MoO 4 2H 2 O and distilled water as raw materials, according to H 3 BO 3 : MnCl 2 .4H 2 O: ZnSO 4 ·7H 2 O: CuSO 4 ·5H 2 O: Na 2 MoO 4 2H 2 O: Distilled water = 286mg: 181mg: 22mg: 7.9mg: 3.9mg: 100ml;

[0022] (2) Prepare BG11 medium, the formula of BG11 medium is that every 1L of medium contains NaNO 3 : 150mg, MgSO 4 ·7H 2 O: 7.5mg, NaSiO 3 9H 2 O: 5.8 mg, K 2 HPO 4 : 4mg, CaCl 2 2H 2 O: 3.6 mg, Na 2 CO 3 : 2mg, citric acid: 0.6mg, ferric ammonium citrate: 0.6mg, A5 solution: 0.1ml, the rest is distilled water;

[0023] (3) In the BG11 medium, add the initial density of 10 4 ~10 5 pcs mL -1 Chrysophy...

Embodiment 2

[0028] Centrifuge the golden algae liquid, and add the harvested golden algae to the algae liquids of Microcystis aeruginosa, Sagittarius terrestriale, Chlamydomonas reinhardtii, Chlorella pyrenoidosa and Crescent algae. The initial density of algal cells of each bloom algae is 2~9×10 5 pcs mL -1 , the initial investment density of golden algae is 5×10 4 pcs mL -1 , after culturing for 5 days, the removal rates of algal cells were 100%, 99%, 99%, 89% and 25%. It can be seen that Chrysogenum phagocytosis has phagocytosis on the above five kinds of bloom algae, and the phagocytosis effect on Microcystis aeruginosa, Sagittarius terrestris and Chlamydomonas reinhardtii is particularly obvious.

Embodiment 3

[0030] Mix 10mL golden algae solution (the initial density of golden algae is 2×10 4 pcs mL -1 ) was directly added to the culture solution of Microcystis aeruginosa with different initial densities, the density of Microcystis aeruginosa decreased rapidly, and the change curve of the removal rate was as follows: figure 1 As shown, the removal rate of Microcystis aeruginosa reached more than 99% in 40 hours. At the same time, it was observed that the lower the initial density of Microcystis aeruginosa, the faster the removal rate of the golden algae, and the shorter the time required for the culture medium to become clear. It can be seen that the effect of preventing algae blooms is more obvious when applying golden algae to the initial stage of algae blooms.

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Abstract

The present invention discloses a method for culturing golden algea and an application thereof in controlling blooming algae, which belong to the field of water pollution control technology. The method for culturing golden algea is as follows: golden algea and microcystic aeruginosa are added into the BG11 culture medium; the golden algea lives on the microcystic aeruginosa to grow heterotrophically; after seven to ten days of culturing, the microcystic aeruginosa is gradually eaten up; the golden algea gets into the stable growing period; high-density golden algea liquid is obtained; and thegolden algea liquid is centrifugated in order to harvest the golden algea. The golden algea harvested by centrifugating or the golden algea liquid is directly added into the water body, and the golden algea swallows the blooming algae, so that the overgrowth of the blooming algae can be controlled and the blooming algae can be eliminated. The method which utilizes the golden algea to swallow the blooming algae to prevent the outbreak of the blooming algae and control the growth of the blooming algae is an algae-inhibiting method which is characterized by good ecological safety, cheap materials, economy, high efficiency and easy utilization, and has the advantages of easy golden algea culturing, good algae control effect, etc.

Description

technical field [0001] The invention belongs to the technical field of water pollution control, in particular to a method for cultivating golden algae and its application in controlling algal blooms. Background technique [0002] The excessive nitrogen and phosphorus content in the water body leads to eutrophication of the water body, which in turn leads to the excessive reproduction of algae to form algae blooms, and makes the water body lose its functions such as industrial and agricultural use, landscape, and breeding. The most widely used method of algae inhibition is copper ion (Cu 2+ ) algicides. Although this type of algicide has a significant algicidal effect, it will also kill other aquatic plants and animals in the water body, destroy the aquatic ecological environment, and will produce bioaccumulation effects in the aquatic ecosystem, or increase the amount of water in the lake sediment. content of harmful substances. Therefore, chemical algicides represented b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89
Inventor 胡洪营张薛
Owner TSINGHUA UNIV
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