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Method for preparing dried chlamydomonas products, its products and use thereof

A technology of single-celled algae and dry products, applied in the direction of single-celled algae, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of toxicity of cultured shellfish larvae, toxicity of cultured biological larvae, and the inability to use shellfish to raise seedlings and other problems, to achieve the effect of complete algae cells, low algae body damage rate, and reduced damage to the water environment

Inactive Publication Date: 2007-08-22
MARICULTURE INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method has many deficiencies: the one, need cryopreservation and volume is bigger; The 2nd, storage time is short, and storage 8 months main nutrient loss reaches 50%; More or less toxic to the larvae of farmed organisms
Such concentrated products are toxic to cultured shellfish larvae and cannot be used for shellfish nursery

Method used

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Experimental program
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preparation example Construction

[0014] The preparation method of the present invention: firstly concentrate the Phytoplankton algae fluid in the growth index phase of conventional, intensive or high-density culture (including autotrophic, heterotrophic and polyculture), which may include primary concentration and final concentration, Among them, the primary concentration can use one of the three methods of natural floating concentration, microfiltration machine concentration or disc separator concentration, and the concentration density can reach 10 to 500 times the original density; then use a tubular centrifuge or a desktop large-capacity low-speed centrifuge Perform final concentration on the concentrated algae liquid after primary concentration (the high-density cultured single-celled algae liquid with a culture density that is more than 5 times higher than the conventional large-scale culture density can also be directly concentrated in the final stage), and concentrated into a paste ; Then carry out low...

example 1

[0025] Take the conventionally cultured flat algal liquid (density ≥ 2 × 10 5 / ml) into an open large pool (tank), the depth of the pool (tank) is 60-100cm, the depth of the algae liquid is 40-80cm, adjust the light to a light intensity of 5000-10000Lux, and manually skim after standing for 1-2 hours Put the algae liquid on the surface into a small container and inflate it for standby (storage time should not exceed 30 hours); to skim off a certain amount (0.8 ~ 1.5M 3 ), use a peristaltic pump to pump the concentrated algae liquid into a tubular centrifuge for centrifugation, the centrifugal speed is 8000-10000rpm, and the continuous centrifugation time is 1-2h each time; 8 / g) Take it out from the separation cylinder and spread it on a polyethylene film, with a thickness of ≤1cm. The polyethylene film is placed in a rectangular shallow pan (height 3cm), and the shallow pans are stacked staggeredly and dried in a drying oven. The drying temperature is 65°C. After complete d...

example 2

[0027] Isochrysis 3011 cultured in high-density photoreactor, density ≥ 1×10 7 / ml, use a peristaltic pump to pump the concentrated algae liquid into a tubular centrifuge (separation) machine for centrifugation, the centrifugal speed is 3500rpm, and the continuous centrifugation time is 0.5-1h each time; after the centrifugation, take out the concentrated algal extract from the separation cylinder and spread it on a polyethylene film On, thickness ≤ 1cm. Put the polyethylene film in a rectangular shallow dish (height 3cm), put it into a vacuum drying oven for drying, the drying temperature is 40°C, and the vacuum pressure is 0.085Mpa; after it is completely dry, peel the dry product from the polyethylene film, put Packed in aluminum foil bags filled with nitrogen.

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Abstract

Disclosed are a preparing method and its product and application. Firstly, condense the pelagic unicellular alga in 1~2 degree, which is made into cream-condensed alga product, the dry it in low temperature or in vacuum, making the products which will not become harmful substance to juvenile of animal culture and can be diffused into alga juvenile of animal culture single cell with complete cell, finally, package it in vacuum or in nitrogen. The invention can be applied in all kinds of pelagic unicellular alga, and the products includes chlorella, nitzschia, chaetocero, can replace live pelagic unicellular alga to be applied in culture of seedling of animal culture in water, cultivation and strengthening of diet animal, especially for feeding diet animal .

Description

technical field [0001] The invention relates to a preparation method of a single-celled algae dry product, a product and an application thereof. Background technique [0002] At present, the supply of single-celled algae in the nursery stage of marine aquaculture animals in my country is mainly the live single-celled algae cultivated by the seedling manufacturers themselves. Because the cultivation of Unicellular algae is often restricted by various factors, there is often a shortage of bait during seedling cultivation, which affects the stability of artificial seedling cultivation. Especially for shellfish larvae that can only feed on the unicellular algae, the situation is particularly serious, and even cause seedling failure. People try to cultivate Unicellular algae in advance in the non-seedling season, and then the algae liquid is concentrated by drug precipitation, stored in a cold storage, and taken out for use during the seedling period. But this method has many d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12A23K1/00C12R1/89A23K10/18A23K50/80
Inventor 杨秀生詹冬梅
Owner MARICULTURE INST OF SHANDONG PROVINCE
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