2603 results about "Preservation methods" patented technology

The invention discloses a blockchain-based evidence preservation method, which is applied to a network system with master nodes and slave nodes, including: each slave node performs identity verification to the master node, and assigns a pair of secret keys to the slave nodes that pass the identity verification. key, and write the registration information of the slave node into the blockchain; after the master node responds to the corresponding slave node with the evidence upload request received, the slave node signs the electronic evidence to be uploaded with the corresponding private key and sends To the master node; according to the registration information of each slave node in the blockchain, verify the legitimacy of the slave node and the authenticity of the signature of the corresponding electronic evidence, store the verified electronic evidence to the cloud storage server and generate the corresponding digital fingerprint, Write the generated digital fingerprint into the blockchain along with the corresponding timestamp. The evidence preservation method of the present invention has strong real-time performance, can save a lot of time, and does not require the participation of a third-party notary organization, thereby saving social resources.

The present invention relates to the storage agent for preservation of an animal cell or organ and preserved process. Ordinary method of cell storage is employed preserving method by freezing at extra low temperature of -196° C. and the survival ratios of cells after thawing and fusion is low, about 10 to 30%. The period of validity is a very short time of 12 to 72 hours. The storage agent can make protein stabilize to protein type storage agent, and prevent, treat and improve organ injury caused on an organ transplant operation by adding the polyphenol.

The present invention concerns the use of oxygen antagonists for inducing stasis in cells. It includes methods and apparatuses for achieving stasis in cells, so as to preserve and / or protect them. In specific embodiments, preservation methods and apparatuses for storing platelets is provided.

The invention provides a cell cryo-preserved liquid, an application, and an immune cell cryo-preservation method. The cell cryo-preserved liquid includes 0.7-0.9 ml / ml of a cell culture medium, 8-26 mg / ml of non-essential amino acids, 0.04-0.1 ml / ml of trehalose, 0.5-10 mg / ml of vitamin C, 10-50 mg / ml of human albumin, 0.04-0.12 ml / ml of propylene glycol, 0.01-0.07 ml / ml of dimethyl sulfoxide and 0.5-3 mg / ml of lentinan. Compared with a cell cryo-preserved liquid in the prior art, the human albumin can replace fetal calf serum to achieve the effects of the fetal calf serum in the cell cryo-preserved liquid, and meanwhile, the cell cryo-preserved liquid is added with the non-essential amino acids, the vitamin C and the lentinan, thereby ensuring cell proliferation vitality after recovery is not influenced. The trehalose and the propylene glycol can replace a protective effect of dimethyl sulfoxide, thereby ensuring moisture in cells to be not crystallized during approach of freezing point. The components are cooperated with each other to achieve cryo-preservation of immune cells.

The invention provides an electric cooker and a heat preservation method thereof. The heat preservation method of the electric cooker comprises the following steps that: a control unit receives a preset heating mode selected by the user from multiple preset heating modes; a temperature detection unit obtains the temperature of a heating area in a liner; and a control unit receives the temperature of the heating area, and controls the heating device according to the preset heating mode selected by the user to preserve heat of the food contained in the liner according to the preset heat preservation curve. According to the invention, the heating device is controlled according to the heat preservation mode selected by the user to preserve heat of the food contained in the liner according to the preset heat preservation curve, the heat preservation needs for different functions can be met, and the heat preservation temperature is dynamically set according to the external input and the conditions in the electric cooker so as to solve the problem that the electric cooker of the prior art cannot dynamically set the heat preservation temperature according to the conditions inside and outside.

The present invention concerns the use of oxygen antagonists for inducing stasis in tissue, including all or part of organs. It includes methods and apparatuses for achieving stasis in tissue, so as to preserve and / or protect them. In specific embodiments, preservation methods and apparatuses for preserving tissue for transplantation purposes is provided.

An antistaling method for the packed food features that a flow control system is coupled to the packing machine to quantitatively spray the antiseptic antistaling liquid onto the surface of said food or in the packing bag.

The invention provides a preservation method for an electronic file. The preservation method comprises the following steps: step 102) generating an electronic evidence packet for the electronic file, marking a time stamp on the electronic evidence packet and then storing the electronic evidence packet, wherein the electronic file is contained in the electronic evidence packet, and step 104) sending and storing digital fingerprints to an electronic evidence server through a transmission channel, wherein the digital fingerprint of the electronic file is generated by utilizing the electronic file, and the digital fingerprint of the electronic evidence packet is generated by utilizing the electronic evidence packet. Correspondingly, the invention also provides a preservation system and a verification system for the electronic file. By adopting the technical scheme of the invention, relationship between the electronic file and an issuer is enhanced, primitiveness of the electronic file is kept, the electronic file is turned into direct evidence, notarization steps are simplified and the notarization efficiency is promoted.

The present invention discloses an all-natural plant composite preservative and preparation method and preservation method thereof. The preservative comprises, by weight percentages, the following ingredients: 0.02%-4% of star anise extract, 0.01%-3% of thyme extract, 0.01%-2% of origanum vulgare extract, 0.02%-5% of litsea cubeba extract, 0.5%-3% of chitosan, 0.05%-12% of emulsifier, and the balance of distilled water. The preservative prepared by the present invention has excellent effect for inhibiting the rot-causing pathogens appeared during the storage of fruits such as cherry; is easy used; can be used to soak fruits or sprayed directly onto fruits; can reduce the rot rate of cherry by more than 50% after room temperature storage for 7 days, compared with a blank group; can also slowdown the water loss of the cherry, maintain the quality of the cherry, and improve the marketability of the cherry.

A cattle and sheep non-animal-derived frozen semen diluent comprises at least one or the combination of the following ingredients: trehalose, propylene glycol, soybean lecithin, soluble soybean protein, nitrogen-trishydroxymethyl aminoethanesulfonic acid and dodecanoyl sodium sulfate. A production method of a cattle and sheet frozen semen by using the cattle and sheep non-animal-derived frozen semen diluent fully mixes the cattle or sheet semen and the dilutent, lowers the temperature and carries out the preservation. Compared with the prior art, the animal semen dilutent of the invention eliminates the animal-derived ingredients; the preservation method is more practical, the technical effects are better, thus thoroughly eliminating the potential risks of spreading various epidemic diseases between the animals and the people by the animal-derived ingredients in the dilutent. The cattle and sheep non-animal-derived frozen semen diluent can promote the popularization of animal artificial insemination technology to the utmost extent and improve the labor productivity and the economic benefits, thus having great economic and social values.

The invention relates to an aquatic vegetable preservative, which comprises citric acid, calcium chloride, sodium tripolyphosphate and vitamin C solution; the quality percentage concentration of the components are that: citric acid accounts for 0.15 percent to 0.2 percent; calcium chloride accounts for 0.5 percent; sodium tripolyphosphate accounts for 0.3 percent to 0.5 percent ; and vitamin C occupies 0.15 percent to 0.2 percent. The invention can be used in the refreshment of fresh lotus seeds, fresh water bamboo and fresh lotus root. The preservation methods are that: firstly, after the processing of sterilization, the fresh lotus seeds, fresh water bamboo and fresh lotus root are soaked for 15 minutes by the chlorine dioxide in a certain concentration; secondly, the dried fresh lotus seeds, fresh water bamboo and fresh lotus root are soaked and sterilized for 2 hours by the preservative in a certain percentage, and then the free water on which is drained; thirdly, a vacuum packaging or modified atmosphere packaging can be implemented for the materials, and then is arranged in the environment under 0 DEG C to 5 DEG C for storage; the shelf life for the aquatic vegetable is more than or equal to 60 days. After the toxicity test, the preservative is in a relative non-toxic level. The invention has the advantages of safety and high efficiency without sulfite components.

The invention discloses a heat preservation control method of a cooking utensil. The cooking utensil comprises a pot body, an upper cover assembly provided with a steam channel, an opening and closing assembly and a gas pump. The opening and closing assembly is used for opening or closing the steam channel. The gas pump is used for drawing gas in a containing cavity outwards. The heat preservation control method comprises the steps that after the cooking utensil enters a heat preservation stage, the steam channel is closed by the opening and closing assembly, the gas pump is started, the gas pump stops running after running for a first time, the gas pump is started after stopping running for a second time, the process is repeated in this way, and the gas pressure in the containing cavity is maintained within a set range; and after the vacuum state continues for a preset time, the gas pump stops gas drawing, and the steam channel is opened by the opening and closing assembly. According to the heat preservation method of the cooking utensil, heat and water loss in the heat preservation stage is reduced, breeding of germs in a pot is restrained, and the food quality keeping time is prolonged. Running of the gas pump is controlled according to time, the structure is simple, and the cost is low.

The invention relates to a cion treating and preserving method. Annual shoots or secondary shoots of over 0.3cm thickness that grow well and are free from damage by disease and insect are selected and cut off 0.5cm above sprout; cut-off shoots are sterilized and socked in 50ppm of solution of growth regulating agent for green plants for 2h, taken out and dried by airing, then mixed with commercial wax of 54 degrees, refined lard and colophony, ratio of which is 1:0.5:0.2, and heated to 100-200 DEG C; then the cion acquired is dipped into wax, wrapped by white thin film and put into a refrigerator for 1-6 months at the temperature of 0 DEG C to 4 DEG C; the cion is taken out, heated and transplanted as by a normal method in due time for grafting. Cion preservation: wax-sealed cion is stored at the temperature of 0 DEG C to 4 DEG C for further use; grafting is carried out when tree sap flows. The invention can prolong the preservation time of the cion and improve grafting survival rate; branches qualified for grating are treated in combination with winter pruning, and are taken out and grafted in due time.

The present invention provides methods for providing cartilage-containing tissue for grafting, comprising providing excised cartilage-containing tissue; and treating said excised cartilage-containing and cryogenically preserving the treated cartilage-containing tissue under appropriate cryogenic preservation conditions so as to yield cryogenically preserved cartilage-containing tissue having at least 10% viable chondrocytes throughout the cartilage portion of the cartilage-containing tissue after preservation, as tested in a live / dead ratio assay. Treatment may comprise providing one or a plurality of incisions in said cartilage portion to a predetermined depth therein and / or introducing a cryoprotectant agent at least into said cartilage portion. The invention also provides viable cartilage obtainable by the methods of the invention, methods of grafting such preserved, viable cartilage containing tissue in a recipient, as well as apparatuses, vessels and systems for preparing a cartilage-containing tissue for cryogenic preservation and subsequent grafting in a recipient.

The invention provides a method for storing mesenchymal stem cells and a method for culturing mesenchymal stem cells. The storing method comprises the following steps of: cutting the umbilical cord into blocks, and putting the blocks into a mixed solution of freezing storage solution and base solution in a volume rate of 1:3 for pretreatment; putting the blocks in the mixed solution of the freezing storage solution and the base solution in a volume rate of 1:1 for treatment at the temperature of 0 DEG C; putting the blocks in the freezing storage solution for treatment at the temperature of 0DEG C; and freeze-storing the blocks in liquid nitrogen. The culturing method is that the stored umbilical cord blocks are used for culture. After babies are delivered, the expensive cell culturing operation of the umbilical cords is not required immediately, the umbilical cords can be directly frozen at the deep low temperature, and the cell activity is not affected. One freezing storage tube can be picked up at any time, the umbilical cords are recovered and successfully cultured and amplified to obtain MSCs, so that the culture operation is not required to be made for each portion of umbilical cord, and the operating cost of the umbilical cord MSCs storage warehouse is greatly lowered.

A method and system for improving the data security of cloud computing comprising: users establishing an index information table for physical LUN devices available to cloud computing service instances, and setting mapping rules of virtual LBA address space for virtual LUN devices and physical LBA address space for data storage according to the index information table; according to the mapping rules, users establishing and saving a mapping relationship between virtual LBA address space and physical LBA address space for data storage; according to the mapping relationship, acquiring storage position information of actual data mapping to the virtual LBA address space pointed by read / write requests, and completing I / O redirection. The system includes an establishment module, setting module, establishment and saving module, and redirection module. The invention enables data owners to master metadata generation method, preservation method and position, and LUN devices of user data not to be illegally mounted, thus guaranteeing security of user data.

The invention discloses a cryoprotective agent of peripheral blood mononuclear cells. The cryoprotective agent is prepared from the following ingredients in parts by weight: 43-53 parts of a culture medium, 30-40 parts of auto-plasma, 12-17 parts of dimethyl sulfoxide, 5-7% of human serum albumin, and the balance being 0.17-0.23mol / L trehalose. The invention discloses a preservation method of the cryoprotective agent. The preservation method comprises the steps of collecting fresh anticoagulation blood, preparing peripheral mononuclear cells into cell suspension by using human serum albumin, adding the cryoprotective agent with the same volume as that of the cell suspension, mixing uniformly, preserving for 10-20h at below 90-below-70-below DEG C, and preserving in liquid nitrogen. According to the cryoprotective agent and the preservation method thereof, a cryopreserved cell has the high motility rate and the strong cell activity and can shorten the Cytokine Induced Killer (CIT) cell induced amplification period.

The invention discloses an audio and video evidence preservation method and system. The method comprises the following steps: verifying an audio and video original file uploaded by a mobile terminal;when the verification passes, generating a complete data fingerprint of the audio and video original file, sending a storage address of the audio and video original file, user information corresponding to the audio and video original file and the complete data fingerprint to a block chain network, and updating storage contents in an authoritative certification node. By adoption of the method, theformation time of the audio and video files and the instantaneity, directness, authenticity, reliability and storage security of recorded contents can be proved, therefore the audio and videos recorded by mobile terminals become credible judicial evidence.

The invention relates to an ultra-low temperature preservation method for oil palm pollen, which comprises the following steps of: drying the collected fresh oil palm pollen after impurity removal to control the residual moisture content of the oil palm pollen to between 8 and 9 percent; filling the dried oil palm pollen into a freezing pipe, directly putting the freezing pipe into liquid nitrogen to perform pre-freezing treatment, then transferring the freezing pipe to an ultra-low temperature refrigerator for preservation, and flushing the ultra-low temperature preserved oil palm pollen by using normal-temperature tap water during de-freezing. The method has simple process and low cost, effectively realizes long-term preservation of the oil palm pollen by preserving the oil palm pollen at the ultra-low temperature on the premise of controlling the residual moisture content of the pollen, and has the advantages of low preservation cost, long storage time, high pollen germination rateand the like.

The invention provides a preparation method of clinical application-level placental hematopoietic stem cells. The preparation method comprises the steps of pretreating a fresh delivered placenta immediately and then separating out umbilical arteries and umbilical veins, pouring a cleaning fluid into the chorionic vessels of the placenta and colleting the cleaning fluid after pouring, pouring an enzyme digestion fluid into the chorionic vessels of the placenta and digesting at 37 DEG C for 5-20min, pouring the collected fluid into the placenta and collecting the liquid after pouring, centrifuging the obtained fluid and re-suspending cells after removing the supernatant, and separating the resuspended cell suspension through a two-step process with hydroxyethyl starch and a lymphocyte separation medium to obtain the clinical application-level placental hematopoietic stem cells. The method is capable of increasing the number and the activity of the prepared placental hematopoietic stem cells and the content of CD34 positive cells, and the prepared placental hematopoietic stem cells are at the clinical application level and free of potential pathogenic contamination, and moreover, the method is low in preparation cost.

The invention discloses a heat treatment method for low-loss and medium and high-frequency iron-based nanocrystalline transformer iron cores. The heat treatment method is characterized by winding iron-based nanocrystalline tapes to obtain iron cores with a duty factor of not less than 75%, and performing first heat treatment on the iron cores in a nitrogen or argon protective atmosphere or a vacuum environment, wherein the first heat treatment adopts a three-stage heat preservation method; performing iron core loss testing on the iron cores after the first heat treatment under conditions of 20kHz and 0.5T, wherein the iron cores with the loss of not more than 22W / kg are first-class iron cores and the iron cores with the loss of more than 22W / kg are second-class iron cores; and performing second heat treatment on the two types of iron cores respectively, wherein the iron cores after the second heat treatment are the low-loss and medium and high-frequency iron-based nanocrystalline transformer iron cores. By adopting the heat treatment method, the medium and high-frequency transformer magnetic cores meeting user requirements can also be prepared by using the relatively thick ordinary iron-based nanocrystalline tapes produced in batches and the qualification rate is greatly improved in comparison with that of the existing methods.

A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering the stored biological material from storage. The invention also features a method of culturing a cell in vitro using a hypertonic medium. Carbohydrate sugars such as trehalose, sucrose, fructose, dextran, and raffinose, may be used as bio-protective agents or in the culture medium.

To provide a cell preservation solution that enables cells to be preserved not only in cryopreservation but also in cold storage, and improves the performance of cell preservation, and also provides a simple cell preservation method using the preservation solution. The cell preservation solution is characterized in that it contains at least carbohydrates, sodium ions and potassium ions, and the molar concentration of the sodium ions is equal to or greater than the molar concentration of the potassium ions. In this way, the cells can be preserved not only by cryopreservation but also by cryopreservation, thereby providing a convenient cell preservation method. Furthermore, with the cell preservation solution of the present invention, cells can be suspended in the preservation solution and directly inoculated in a culture medium for cell culture without going through steps such as washing, so it is very convenient.

A method for cryopreservation of cells characterized by the use of a solution containing, as essential components, a sodium salt, a potassium salt, a saccharide, a cryoprotectant, and a hydrogencarbonate salt and / or a carbonate salt, the solution further optionally containing a component selected from the group consisting of proteins, magnesium salts, and calcium salts. According to the present invention, a method for cryopreservation of cells capable of maintaining a high viability rate after thawing, and a cryopreservation solution used in the cryopreservation are provided. In addition, by using the method for cryopreservation, the cells can be preserved in a state of maintaining a high viability rate even after thawing, and also maintaining their physiological functions; further, a cell washing step after freezing and thawing is unnecessary, thereby making it very useful in the fundamental researches of cells and the application studies on the medical treatment.

The invention provides a method of specialized random access resource reservation. In the method, the required time of the terminal random access failure is estimated, and according to the required time of the terminal random access failure, the reserved period of specialized random access resources is determined; when the random access process is triggered, the base station distributes specialized random access resources for the terminal according to the reserved period. The invention simultaneously provides a base station for realizing the specialized random access resource reservation, and the method and the base station can avoid unnecessary specialized random access resource reservation and improves the utilization rate of the specialized random access resources.

The invention relates to a method for artificial culture and propagation of plant root-knot nematodes and for species preservation. The propagation and preservation of the root-knot nematodes in a lab depend on host plants all the time; the invention places a relatively small amount of root-knot nematode ovums or 2nd instar larvas in a particular dual culture medium rather than host roots and performs a upside-down culture under conditions of constant temperature and hermetic closure, the root-knot nematodes ingest, grow and propagate inside the culture medium and a large amount of nematomorphas can be obtained in a short term; additionally, a culture plate is timely transferred to be located under cold conditions, and dish change and subculture are implemented once within a half year so that the propagation and preservation processes of the root-knot nematodes are carried out without the host plants. The inventive advantages lie in: saving the culture program of the root-knot nematodes to host the plants without losing the infectivity of the root-knot nematodes on sensitive host plants; the employed materials have low costs and the experiment method is easy in operation; the invention provides a simple, convenient and fast propagation method for the species preservation of the root-knot nematodes, in particular for a large number of nematomorphas as required in the related researches of the root-knot nematodes.

The invention discloses a color protection preservation method of a poached green leaf vegetable instant product, belonging to the field of fruit / vegetable food processing. The method mainly comprises the following steps of: performing pretreatment, blanching for enzyme deactivation and vacuum infiltration soaking with a color protection and crisp protection agent (sodium alginate, calcium chloride, potato starch and zinc gluconate) of the green leaf vegetable; and performing secondary color protection preservation soaking by use of the color protection preservation liquid consisting of table salt, edible glycerin, sodium carboxymethylcellulose and sucrose ester. The method reduces the color protection preservation cost, and improves the color of the poached green leaf vegetable product stored in normal-temperature condition. The nano ZnO antibacterial liquid (in which a sodium hexametaphosphate dispersion protector with concentration of 0.015% is added) is subjected to vacuum packing and low-frequency (915MHz) microwave uniform sterilization. The storage temperature is normal temperature. Through the invention, good color and fresh degree of the poached green leaf vegetable instant product are kept in the normal-temperature storage period.