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Ultra-low temperature preservation method for oil palm pollen

A technology for ultra-low temperature preservation and pollen, which is applied to the preservation of plants, botanical equipment and methods, applications, etc., can solve the problem of high cost of pollen, and achieve the effects of long-term preservation, low preservation cost and simple process

Inactive Publication Date: 2010-12-29
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its main disadvantage is that the cost of pollen preservation is too high

Method used

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  • Ultra-low temperature preservation method for oil palm pollen
  • Ultra-low temperature preservation method for oil palm pollen
  • Ultra-low temperature preservation method for oil palm pollen

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Embodiment Construction

[0012] The operation process of the present invention will be further described below, and related research results of the present invention will be introduced.

[0013] 1. Operation process: Collect fresh pollen at 9-11 am on a sunny day. According to the transportation distance or length of time, put the pollen in an ice box or a plastic sealed bag and bring it back to the laboratory. The fresh pollen is sieved with an 80-100 mesh sieve. After miscellaneous, use a measuring spoon to measure the sieved pollen and pour it into the sieve of the above specification, shake off the pollen evenly on sulfuric acid paper or a petri dish lined with sulfuric acid paper, and then put it into a constant temperature drying box at 37-40°C Dry the pollen for more than 12 hours, so that the remaining water content of the pollen is 8-9%. Put the above-mentioned oven-dried pollen (about 0.5 g) into a 2ml internal thread freezing tube, tighten the tube cap and put it directly into liquid nitroge...

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Abstract

The invention relates to an ultra-low temperature preservation method for oil palm pollen, which comprises the following steps of: drying the collected fresh oil palm pollen after impurity removal to control the residual moisture content of the oil palm pollen to between 8 and 9 percent; filling the dried oil palm pollen into a freezing pipe, directly putting the freezing pipe into liquid nitrogen to perform pre-freezing treatment, then transferring the freezing pipe to an ultra-low temperature refrigerator for preservation, and flushing the ultra-low temperature preserved oil palm pollen by using normal-temperature tap water during de-freezing. The method has simple process and low cost, effectively realizes long-term preservation of the oil palm pollen by preserving the oil palm pollen at the ultra-low temperature on the premise of controlling the residual moisture content of the pollen, and has the advantages of low preservation cost, long storage time, high pollen germination rateand the like.

Description

technical field [0001] The invention relates to a method for preserving pollen, in particular to an ultra-low temperature preservation method for oil palm pollen. Background technique [0002] Oil palm is a perennial monocotyledonous plant. It is a tropical woody oil crop. Its pulp and kernels are rich in oil. It is known as the "World Oil King". Palm oil is mainly used to make soap, lubricants, cosmetics, etc. Tanning industry, auxiliary agent for tin plating, etc. After palm oil is refined, it is edible oil with high nutritional value, which can be used to make margarine and has high economic value. Oil palm is a cross-pollinated plant, with male and female inflorescences open alternately, usually pollinated by wind and insects. Pollination is one of the important factors affecting the yield of oil palm. It is mainly manifested in: (1) Insufficient pollination leads to a decrease in yield, especially in young oil palm gardens; (2) The number of male flowers and the ratio ...

Claims

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Application Information

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IPC IPC(8): A01N3/00
Inventor 曾宪海尤丽莉邹积鑫林位夫谢贵水安锋陈俊明张希财王军
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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