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Endophytic fungi chaetomium globosum strain, microbial agent and application thereof

A technology of plant endophytic fungi and microbial inoculants, which is applied in the field of agricultural microorganisms, can solve problems such as affecting field biocontrol effects, unstable disease control effects, and easy loss of vitality, and achieves growth promotion, long storage time, and improved adaptation. sexual effect

Inactive Publication Date: 2012-01-11
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application in production has achieved certain effects, the field application often leads to unstable disease control effects due to the influence of various environmental factors
The main reason is that the spore powder preparation of Trichoderma is its asexual conidia, which is easy to lose vitality and has a short shelf life, which seriously affects the biocontrol effect in the field.

Method used

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  • Endophytic fungi chaetomium globosum strain, microbial agent and application thereof
  • Endophytic fungi chaetomium globosum strain, microbial agent and application thereof
  • Endophytic fungi chaetomium globosum strain, microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Example 1: Field test of Chaetomium globosum ND35 strain promoting the growth of walnut and chestnut seedlings

[0118] The experiment was carried out in the nursery of Guanghuasi forest area of ​​Lulaishan Forest Farm in Tai'an City from 2007 to 2010. Firstly, evenly spread organic fertilizer on the surface of the field, turn the ground deeply, level it, ridge the ridge, the width of the border is about 1m, adjust the ditch (depth 15cm) and water, select the walnut seeds with good germination and place them in the ditch. The row spacing is 20cm×30cm, and the completely random arrangement makes the germination status in each treatment roughly the same. The test is divided into two treatments. Treatment ①, the germinated walnut seeds are inoculated with a solid inoculum for cultivating chaetomium ND35 strain, about 5g per seed. Treatment ②, take blank as control. All add appropriate concentration of phoxim to control underground pests. After the walnut seedlings were gr...

Embodiment 2

[0130] Example 2: Inducing disease resistance effect of Chaetomium globosum ND35 strain on poplar cutting seedlings

[0131] The experiment was carried out at the Experimental Station of the Plant Protection College of Shandong Agricultural University South Campus from June to October 2008.

[0132] (1) Field experiment to investigate the antagonistic effect of Chaetomium globosum ND35 on the induction of poplar leaf rust

[0133] Due to environmental factors and poplar species, poplar cuttings were infected with leaf rust in June. In August, the disease on the front side (Dendrum spore pile) and the back side (Umster spore pile) of the naturally susceptible poplars of the inoculated and control groups were investigated at the poplar 1.3, 1.7, 2.1m respectively. In poplars with disease, the grading standard is determined according to the ratio of the area of ​​the diseased spots on the front and back of the leaf to the total leaf area at the same height: 0-20% of the disease is grad...

Embodiment 3

[0146] Example Three, the effect of chaetomium ND35 strain on the growth of ginger

[0147] The experiment was conducted at the Experimental Station of the College of Horticulture Science and Engineering, South Campus of Shandong Agricultural University. The ginger was germinated and sown on May 5, 2010, with a row spacing of 65cm and a plant spacing of 20cm. The test is divided into two treatments: ①: birth control bacteria, ②: blank control. Other field management is carried out as usual [7] . By the time of harvest on October 19, five trees were randomly selected in groups, and the stem thickness, plant height, number of branches, number of leaves, leaf fresh weight, stem fresh weight, and underground fresh weight were determined.

[0148] Growth rate = (average per plant in the treatment group-average per plant in the control group) / average per plant in the control group × 100%

[0149] The test results showed that the plant height, stem thickness, number of branches, number o...

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Abstract

The invention discloses an endophytic fungi chaetomium globosum strain, a microbial agent and an application thereof. The strain is named as chaetomium globosum strain ND 35, the preservation organization is the China General Microbiological Culture Collection Center, the preservation date is 9th,10,2010, the preservation number is CGMCC No. 4136. The strain is a biological beneficial bacteria with a plurality of biological control effect mechanisms, and has the plant pathogen inhibition capability with broad-spectrum and high-efficiency, and also has capability for promoting a plurality of plants growth, and is suitable for industrial production, the shelf life is long which is suitable for preventing and treating the plant diseases, so that the strain is used for producing the biological bactericide products for preventing and treating a plurality of plant diseases and multifunctional biofertilizer products for promoting the growth of plants.

Description

Technical field [0001] The present invention belongs to the field of agricultural microorganisms. The Chaetomium globosum ND35 strain is a plant endophytic fungus strain with multi-mechanism and broad-spectrum inhibition of plant pathogenic bacteria and the ability to promote plant growth. Biological fungicide products for plant diseases and multifunctional biological fertilizer products for promoting plant growth. Background technique [0002] The use of live microbial agents to control plant diseases is one of the traditional biocontrol technologies. Currently, the most commercially available biocontrol fungi are Trichoderma. Although the application in production has achieved certain results, the field application often leads to unstable disease prevention effects due to the influence of various environmental factors. The main reason is that the spore powder preparation of Trichoderma is asexual conidia, which is easy to lose vitality and has a short shelf life, which serious...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12N3/00C12R1/645
Inventor 高克祥刘晓光米士伟毕建杰张修国
Owner SHANDONG AGRICULTURAL UNIVERSITY
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