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Cell-preservation liquid,and preparing method thereof,cell preservation method and cell culturing method

A preservation method and preservation solution technology, applied in tissue culture, biochemical equipment and methods, microorganisms, etc., can solve problems such as insufficient effect and unstable preparation, and achieve the effect of maintaining cell physiological functions and improving cell survival rate

Inactive Publication Date: 2006-05-17
NIPRO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these solutions have problems such as insufficient maintenance of cell physiological functions and instability in formulations.

Method used

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  • Cell-preservation liquid,and preparing method thereof,cell preservation method and cell culturing method
  • Cell-preservation liquid,and preparing method thereof,cell preservation method and cell culturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] (Example 1) cell preservation solution containing glucose and sodium bicarbonate

[0097] Prepare a cell preservation solution consisting of the following components. The osmotic pressure is in the range of 280-300 mOsm / kg. The osmotic pressure was measured using OSMOMETER OM 802-D (manufactured by VOGEL).

[0098] Glucose (manufactured by Wako Pure Chemical Industries, Ltd.) 5.5mM

[0099] Sodium ion 143.8mM

[0100] Potassium ion 5.4mM

[0101] Bicarbonate ion and / or carbonate ion 23.7mM

[0102] Calcium ion 1.8mM

[0103] Magnesium ion 0.8mM

[0104] Chloride ion 130.8mM

[0105] Phosphate ion 1.0mM

[0106] Sulfate ion 0.8mM

[0107] pure water

[0108] Total 1,000mL

Embodiment 2

[0109] (Example 2) cell preservation solution containing maltose and sodium bicarbonate

[0110] The same cell preservation solution as in Example 1 was prepared except that the glucose in Example 1 was replaced with maltose (manufactured by Wako Pure Chemical Industries, Ltd.).

Embodiment 3

[0111] (Example 3) cell preservation solution containing mannitol and sodium bicarbonate

[0112] A cell preservation solution similar to Example 1 was prepared except that glucose in Example 1 was replaced with mannitol (manufactured by Wako Pure Chemical Industries, Ltd.).

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Abstract

To provide a cell preservation solution that enables cells to be preserved not only in cryopreservation but also in cold storage, and improves the performance of cell preservation, and also provides a simple cell preservation method using the preservation solution. The cell preservation solution is characterized in that it contains at least carbohydrates, sodium ions and potassium ions, and the molar concentration of the sodium ions is equal to or greater than the molar concentration of the potassium ions. In this way, the cells can be preserved not only by cryopreservation but also by cryopreservation, thereby providing a convenient cell preservation method. Furthermore, with the cell preservation solution of the present invention, cells can be suspended in the preservation solution and directly inoculated in a culture medium for cell culture without going through steps such as washing, so it is very convenient.

Description

technical field [0001] The invention relates to a cell preservation solution, which is characterized in that it includes at least carbohydrates, sodium ions and potassium ions, and the molar concentration of the sodium ions contained therein is equal to or greater than the molar concentration of the potassium ions. method of cell preservation. Background technique [0002] Living cells have physiological functions and biological activities, and have application value in many aspects. However, if it is placed under high temperature conditions, it will change over time due to metabolism and modification, and the physiological functions and biological activities of cells will be reduced or lost. Therefore, cells must be cryopreserved. For a long time, cell membrane permeable cryopreservatives such as DMSO (dimethyl sulfoxide), ethylene glycol, and glycerin have been used for cell cryopreservation, and cell membrane impermeable cryopreservatives such as polyvinylpyrrolidone ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
Inventor 田口淳史佐藤威矢部则次
Owner NIPRO CORP
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