241results about How to "Inhibit enzyme activity" patented technology

A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.

The present invention provides a compound having a superior acid secretion inhibitory action, an antiulcer activity and the like.A proton pump inhibitor containing a compound represented by the formula (I)wherein ring A is a saturated or unsaturated 5- or 6-membered ring group optionally having, as a ring-constituting atom besides carbon atom, 1 to 4 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom, ring-constituting atoms X1 and X2 are each a carbon atom or a nitrogen atom, a ring-constituting atom X3 is a carbon atom, a nitrogen atom, an oxygen atom or a sulfur atom, R1 is an optionally substituted aryl group or an optionally substituted heteroaryl group, R2 is an optionally substituted alkyl group, an optionally substituted aryl group or an optionally substituted heteroaryl group, R3 is an aminomethyl group optionally substituted by 1 or 2 lower alkyl groups, which is a substituent on a ring-constituting atom other than X1, X2 and X3, and ring A optionally further has substituent(s) selected from a lower alkyl group, a halogen atom, a cyano group and an oxo group, or a salt thereof or a prodrug thereof.

Provided is a compound having a superior acid secretion suppressive action, which shows an antiulcer activity and the like.A compound represented by the formula (I) or a salt thereof:wherein ring A is a saturated or unsaturated 5-membered heterocycle containing, as a ring-constituting atom besides carbon atoms, at least one heteroatom selected from a nitrogen atom, an oxygen atom and a sulfur atom, the ring-constituting atoms X1 and X2 are the same or different and each is C or N, the ring-constituting atoms X3 and X4 are the same or different and each is C, N, 0 or S (provided that a pyrrole ring wherein X1 is N is excluded from ring A), and when the ring-constituting atom X3 or X4 is C or N, each ring-constituting atom optionally has substituent(s) selected from an optionally substituted alkyl, an acyl, an optionally substituted hydroxy, an optionally substituted mercapto, an optionally substituted amino, a halogen, a cyano and a nitro;R1 and R2 are each a cyclic group optionally having substituent(s); R3 and R4 are each H or alkyl, or R3 and R4 form, together with the adjacent N, an nitrogen-containing heterocycle; and Y is a spacer.

The present invention discloses an RNA virus inactivation preservation solution. The inactivation preservation solution comprises ammonium salt, 1-ethyl-3-methylimidazolium bisulfate, lithium chloride, ethylenediaminetetraacetic acid, an RNase inhibitor, a surfactant, a reducing agent, a buffer agent and water. The present invention also provides an RNA virus sample inactivation and preservation method and an RNA virus detection method, and the method is conducted by using the inactivation preservation solution. The preservation solution has low cost, inhibits RNase activity, preserves RNA integrity, realizes preservation and transportation of samples at room temperature, can also quickly inactivate pathogens at room temperature, reduces infection risks of medical personnel, and reduces false negatives in detection. At the same time, the inactivation preservation solution can realize a lysis function, does not contain chaotropic reagents and alcohols, is compatible with mainstream nucleic acid extraction kits on the market, greatly saves time of an entire process, and is particularly suitable for 2019 new coronavirus (COVID-19) inactivation and lysis steps after swab sampling.

The present invention relates to novel classes of compounds which are inhibitors of interleukin-1β converting enzyme. The ICE inhibitors of this invention are characterized by specific structural and physicochemical features. This invention also relates to pharmaceutical compositions comprising these compounds. The compounds and pharmaceutical compositions of this invention are particularly well suited for inhibiting ICE activity and consequently, may be advantageously used as agents against IL-1-, apoptosis-, IGIF-, and IFN-γ-mediated diseases, inflammatory diseases, autoimmune diseases, destructive bone disorders, proliferative disorders, infectious diseases, degenerative diseases, and necrotic diseases. This invention also relates to methods for inhibiting ICE activity, for treating interleukin-1-, apoptosis-, IGIF- and IFN-γ-mediated diseases and decreasing IGIF and IFN-γ production using the compounds and compositions of this invention. This invention also relates to methods for preparing N-acylamino compounds.

The invention discloses a seabuckthorn fruit juice and a preparing method thereof, and belongs to the field of juice processing. The preparing method includes the following steps of (1) raw seabuckthorn juice preparing; (2) material taking, wherein 40-75 by weight parts of the raw seabuckthorn juice, 10-30 by weight parts of white sugar, 1-5 by weight parts of xanthan gum, 3-8 by weight parts of honey, 0.5-3 by weight parts of gelatin, 1-3 by weight parts of aspartame, 40-100 by weight parts of water and 10-30 by weight parts of raw apple juice are taken; (3) preparing and clarifying; (4) sterilizing, wherein sterile clarified liquid is obtained through high-voltage pulse electric field sterilization; (5) seabuckthorn fruit juice obtaining, wherein the seabuckthorn fruit juice is obtained in a sterile-filling mode. By means of the eabuckthorn fruit juice and the preparing method thereof, nutrient ingredients and flavor substances in seabuckthorn fruits can be effectively reserved.

The present inventions relate to novel skin protectant creams and the use of such creams to cleanse, treat, and protect skin. The skin protectant creams can be topically applied to protect skin from irritants, to block the action of irritants such as enzymes, and to treat skin that has been subject to irritants. The creams can be applied manually or incorporated into disposable applicators, such as wipers or swabs. The skin protectant creams of the present invention are particularly useful in the prevention of severe skin breakdown which can lead to partial thickness skin wounds.

The invention discloses a moisturizing composition with an anti-allergy repairing efficacy and preparation and application to skincare products. The composition is prepared from xanthan gum, pullulan,polyglutamic acid, hyaluronic acid, trehalose, allantoin, urea and chondroitin sulfate by reasonable compounding. The composition has a function of stereo moisturizing of all skin layers including acorium layer, an epidermal layer, a cuticle layer and a skin surface, the skin moisture content can be increased, and skin moisture loss is reduced. The composition is capable of inhibiting hyaluronidase activity, soothing skin allergy and pruritus and repairing skin barrier. The composition has excellent moisturizing effects and an anti-allergy repairing efficacy in vitro and on human skin, effects are remarkably improved as compared with those of single use of each component, and accordingly synergistic effects are achieved by combination of the components in an appropriate ratio. The composition is solid powder and free of any preservatives, and convenience in long-time storage and transport is achieved. The composition can be applied to all types of skincare and cleansing products.

The present invention provides 5-membered heterocycle compounds represented by the following general formula (I):The present compounds have a superior acid secretion inhibitory effect, and shows an antiulcer activity and the like.

The invention provides a whitening composition, whitening skin-brightening cream comprising the same and a preparation method thereof. The whitening skin-brightening cream is prepared from the following components in percent by mass: 0.1-20% of the whitening composition, 1-25% of a wetting agent, 1-5% of an emulsifier, 1-20% of skin-moistening grease, 0.1-5% of a skin conditioner, 0.05-3% of a permeation aid, 0.01-1% of a chelating agent, 0.1-1% of a thickener, 0-1% of essence, 0.05-2% of a preservative and the balance deionized water. The whitening composition is prepared from eight traditional Chinese medicinal material extraction solutions: ginseng, cortex mori radicis, angelica sinensis, glycyrrhiza, tea leaves, ganoderma atrum, rhodiola rosea and folium ginkgo as well as conventionalwhitening agents nicotinamide and tranexamic acid, and has the functions of whitening synergically and reducing irritation. The whitening skin-brightening cream moistens the skin and is not greasy, iseasy to absorb, and is obvious in whitening and skin-brightening effect.

The invention relates to full-natural extruded coprinus slices and a production method thereof, belonging to the technical field of agricultural product deep processing. The production method comprises the following the steps that: after slicing and color protection processing, coprinus is put in an air dry oven until the water content of the corpinus is 65-70% of the water content of fresh unopened coprinus; and coprinus slices are placed in a vacuum microwave drying machine to be processed for 3-4min, and then, are returned to be at one air pressure. In the invention, by adopting ozonated water color protection, vacuum microwave drying extrusion, and other technical research and application, during the production process, no any additive is added, processed extruded coprinus slices have beautiful appearances and crisp taste, and are less in nutrition loss, the quality of the product is good, and the full-natural extruded coprinus slices have a wide market development prospect. According to the invention, the defects of fry extrusion and differential pressure extrusion are overcome, and the quick, low-temperature and non-fried extrusion processing of the coprinus slices are realized.

The invention relates to a coating preservation method for eggs. The method comprises the following steps: (1) selecting fresh and crack-free eggs, cleaning the eggs and then adding water to soak the eggs; (2) taking 1 to 2 parts of xanthan gum, 1.6 to 2.05 parts of composite plant essential oil, 0.125 to 0.25 part of licorice flavonoids, 30 to 35 parts of an emulsifier and 50 to 100 parts of ganoderma lucidum polysaccharide, mixing the above-mentioned materials and adding the obtained mixture into water under stirring so as to obtain 1000 parts by weight of an essential oil emulsion; and (3) taking the essential oil emulsion and water according to weight parts, mixing the essential oil emulsion and water so as to obtain a spraying liquid, coating the shells of the eggs with the spraying liquid and drying the eggs at 37 DEG C in the air for preservation. According to the invention, the surface of each egg is coated by a layer of a preservative film which inhibits enzymatic activity of the eggs, alleviates decay of life, achieves the purpose of freshness and quality retention, increases the shelf life of the eggs and saves social cost; moreover, the coated preservative on the surfaces of the eggs is colorless and transparent after drying, so the advantages of safety and low cost are obtained.

The present invention provides a compound having a superior acid secretion inhibitory action, an antiulcer activity and the like.A proton pump inhibitor containing a compound represented by the formula (I)wherein ring A is a saturated or unsaturated 5- or 6-membered ring group optionally having, as a ring-constituting atom besides carbon atom, 1 to 4 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom, ring-constituting atoms X1 and X2 are each a carbon atom or a nitrogen atom, a ring-constituting atom X3 is a carbon atom, a nitrogen atom, an oxygen atom or a sulfur atom, R1 is an optionally substituted aryl group or an optionally substituted heteroaryl group, R2 is an optionally substituted alkyl group, an optionally substituted aryl group or an optionally substituted heteroaryl group, R3 is an aminomethyl group optionally substituted by 1 or 2 lower alkyl groups, which is a substituent on a ring-constituting atom other than X1, X2 and X3, and ring A optionally further has substituent(s) selected from a lower alkyl group, a halogen atom, a cyano group and an oxo group, or a salt thereof or a prodrug thereof.

A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.

This invention relates to the use of cyclooxygenase-2 inhibitors or derivatives thereof in preventing cardiovascular disorders.

The invention provides a method to extract dihydroquercetin from roots of a particular tree species Larix olgensis. The method includes the steps of selection of raw materials, enzyme treatment, extraction, concentration, hydrolysis, extraction, recrystallization, and spray drying. Composition of the flavonoid compounds in the extractive of Larix olgensis is confirmed by HPLC-MS, and the extractive mainly comprises dihydroquercetin, and others compounds of aromadendrin, eriodictyol, quercetin and kaempferol. In vitro experiments prove that dihydroquercetin extractive form Larix olgensis has strong inhibitory effects on tyrosinase activity, and the effect of scavenging DPPH free radicals. Animal in vivo experiment demonstrates that the Larix olgensis extractive has good recovery effect on carbon tetrachloride induced liver injury.

The invention relates to a method used for preparing beet red color with high color value from dragon fruit, and belongs to the technical field of natural pigment. The method comprises following steps: a, dragon fruit is collected, ice blend is added, and an obtained mixture is subjected to pulp making and filtration so as to obtain juice; b, active carbon or diatomite is added into the obtained juice, and an obtained mixed material is stirred, centrifuged, and filtered so as to obtain a pigment solution; c, the obtained pigment solution is processed by using a MCI gel chromatographic column, is subjected to elution with water, and an eluent is collected and dried so as to obtain beet red color. Beneficial effects of the method are that: impurity content of the beet red color obtained via the method is low, color value is high, the method is simple and convenient, and production cost is low.

Ortho methoxy phenolic compounds are provided that include methylenedioxyphenyl ferulate and ferulylproline and derivatives thereof. Pharmaceutical compositions comprising the compounds and methods of using the compounds for treating cardiovascular diseases, including hypertension, atherosclerosis, coronary heart disease, angina, stroke, and myocardial infarction, are further provided. The compounds are also useful in reducing low-density lipoprotein oxidation, improving or increasing vasodilation, and reducing plaque destabilization in a subject.

The feed additive is characterized by that said feed additive is a mixture of pyruvate and creatine monohydrate, in which the content of pyruvate is 55-75 wt%. The can raise thin-meat rate in animal body obvious, and has no harmful substance, is safe for human body.

The invention discloses a preparing method of a kind of rose collagen peptide powder belonging to the field of food resource development and processing technology, which is characterized in that the coniferous fruit powder and the rose oil are added on the basis of the collagen peptide. The method has following advantages: combination of ingredients of the rose collagen peptide powder is reasonable; synergistic interaction of all ingredients is significant without toxic, side effects and additives; the whitening and anti-wrinkling effect, moisturizing and smoothing effect, anti-acne and dark spots effect, and anti-coarse effect are provided; drinking and carrying are convenient; the production process is simple and safe, operability is strong, the production cycle is short, thereby suitable for industrial production.

The invention relates to a method of extracting, purifying and inhibiting an alpha-glucosaccharase active ingredient from a chestnut shell. The method comprises the following steps: firstly, cleaning the chestnut shell, drying, crushing, refluxing and extracting by 75% (v / v) alcohol, concentrating under reduced pressure, adding water and statured n-butyl alcohol liquor to extract; and finally, carrying out adsorption elution by using macroporous resin AB-8 to obtain polyphenol extract with effect of inhibiting activity of alpha-glucosaccharase.

The present invention relates to a process for producing cheese, comprising the steps of: providing a first raw material liquid; providing a second raw material liquid; treating the first raw material liquid with a protein crosslinking enzyme to provide an enzyme-treated raw material liquid; mixing the enzyme-treated raw material liquid with the second raw material liquid to provide cheese milk; processing the cheese milk into cheese. The process produces cheese in improved yields while retaining the organoleptic properties of cheese unchanged. The invention further relates to cheese treated with a protein crosslinking enzyme, having the moisture on a fat-free basis of 67% or less and a protein profile of cheese has proteins of molecular weight of less than 66 kDa.

The invention relates to a Streptomyces griseus subsp.griseus trehalose synthetase gene and applications thereof. The gene is cloned from Streptomyces griseus subsp.griseus, and the synthetase gene expressed by the gene is capable of converting maltose into trehalose. The gene has the biggest characteristics of high conversion efficiency and high reaction speed. Under the reaction condition provided by the invention, the content of the trehalose in the reaction product of the trehalose generated by converting the maltose can reach 87 percent, and moreover, only 3.8 percent of glucose is produced, thereby being beneficial to increasing the conversion rate of a substrate and reducing the production cost. Recombinant trehalose synthetase produced by applying the gene can be used for preparing trehalose by using maltose syrup sold in the market or maltose syrup prepared from starch. The trehalose can be applied to the field of food industry, facial cosmetics, pharmaceutical and biological products and the like.

The invention relates to instant natural fruit and vegetable powder. The instant natural fruit and vegetable powder comprises the following components in parts by weight: 95-99 parts of mixed freeze-dried fruit and vegetable powder, 3.5-5.5 parts of konjac powder and 0-1 part of a siraitia grosvenorii extract. The invention aims to provide the instant natural fruit and vegetable powder which is rich in nutrition, unique in flavor, easy to preserve, convenient to carry and simple to brew.

The invention discloses a whitening and moisturizing stock solution and a preparation method thereof. The whitening and moisturizing stock solution is prepared from nonapeptide-1, hexapeptide-2, vitamin C ethyl ether, nicotinamide and the like, the activity of melanoblast is effectively weakened, the output of melanin is reduced, generation of the melanin is inhibited, the melanin conveying meanscan be inhibited, and the effects of skin color equalization and brightening are achieved; the good antioxidant and antibacterial and anti-inflammatory effects are achieved, synthesis with collagen isconducted, the skin cell activity is repaired, and the elasticity and smoothness of the skin are improved. The barrier function of the skin can be improved, the transepidermal water loss is lowered,and the moisturizing effect of the stock solution is improved; facial redness and pigmentation are reduced; the effect of expanding the blood vessel is achieved.

The invention discloses hippophae rhamnoides jam and a preparation method thereof. The method comprises the following steps of (1) preparing hippophae rhamnoides juice; (2) preparing hippophae rhamnoides pericarp paste; (3) preparing concentrated juice: mixing cucumber juice, apple juice and the hippophae rhamnoides juice at the mass ratio of 1:(2-3):(10-15), and freezing and concentrating until the weight percent of a soluble solid is 30-38%; (4) sterilizing: evenly mixing the separated pulp, pectin, fibers and the hippophae rhamnoides pericarp paste, then conducting pasteurization to obtain a pulp material, and conducting high voltage pulsed electric field sterilization on the concentrated juice to obtain sterile concentrated juice; and (5) taking the following raw materials by weight: 50-70 parts of the sterile concentrated juice, 5-15 parts of the pulp material, 1-5 parts of a stabilizer, and 10-25 parts of a sweetening agent, and mixing the raw materials evenly to obtain the hippophae rhamnoides jam. The hippophae rhamnoides jam prepared through the method is low in loss of nutrients and flavor substances, and is tasty and refreshing.